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Reviewed, UniProtKB/Swiss-Prot Q9SRH4 (PEL7_ARATH)

Last modified November 3, 2009. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable pectate lyase 7
    EC=4.2.2.2
Gene names
Ordered Locus Names: At3g01270
ORF Names: T22N4.10, T4P13.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion. Required for its activity By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Sequence similarities

Belongs to the polysaccharide lyase 1 family.

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Ontologies

Keywords
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pectate lyase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 475451Probable pectate lyase 7
PRO_0000024871

Sites

Active site3511 Potential
Metal binding2711Calcium By similarity
Metal binding2951Calcium By similarity
Metal binding2991Calcium By similarity

Amino acid modifications

Glycosylation671N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict281D → E in AAM60924. Ref.2
Sequence conflict801V → I in AAM60924. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q9SRH4-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: DE1550EA09B8161D

FASTA47553,855
        10         20         30         40         50         60 
METARLFKLV CVICIASLIP TIRANVADET DEYWVNKANE ARKHTLMAYH PDPYEIVDHF 

        70         80         90        100        110        120 
HERHYDNSTD VEGTEEEKAV ASEEEDVIEM ISSPTNSTRR SLTGRGKGKG KGKWSKLTGP 

       130        140        150        160        170        180 
CTASNPIDKC WRCQPDWARR RKKLVHCVRG FGYRTTGGKR GRIYVVTSPR DDDMVNPRPG 

       190        200        210        220        230        240 
TLRHAVIQKE PLWIVFKHDM SIRLSQELMI TSDKTIDARG ANVHIAYGAG ITMQYVHNII 

       250        260        270        280        290        300 
IHGLHVHHIV KSSGGLIRDS INHFGHRGEA DGDGISIFGA TNIWLDHISM SKCQDGLIDA 

       310        320        330        340        350        360 
IMGSTAITIS NSHFTHHNDV MLLGAQNNNM DDKKMQVTVA YNHFGKGLVQ RMPRVRWGFV 

       370        380        390        400        410        420 
HVVNNDYTHW ELYAIGGSQG PTILSHGNRF IAPPHKQHYR EVTKRDYASE SEWKNWNWRS 

       430        440        450        460        470 
EKDVFMNNAY FRQSGNPHFK CSHSRQQMIK PKNGMAVSKL TKYAGALDCR VGKAC

« Hide

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References

[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

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Cross-references

Sequence databases

AC010676 Genomic DNA. Translation: AAF03499.1.
AC008261 Genomic DNA. Translation: AAF26147.1.
AY084341 mRNA. Translation: AAM60924.1.
IPIIPI00532794.
RefSeqNP_186776.1.
UniGeneAt.18310

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyPL1. Polysaccharide Lyase Family 1.

Genome annotation databases

GeneID821142.
GenomeReviewsGene locus AT3G01270 in contig BA000014_GR.
KEGGath:AT3G01270.
NMPDRfig|3702.1.peg.12043.

Organism-specific databases

TAIRAt3g01270.

Phylogenomic databases

OMAVTISNCH.

Enzyme and pathway databases

BRENDA4.2.2.2. 302.

Gene expression databases

ArrayExpressQ9SRH4.
GenevestigatorQ9SRH4.
GermOnlineAT3G01270. Arabidopsis thaliana.

Family and domain databases

InterProIPR002022. Amb_allergen.
IPR018082. AmbAllergen.
IPR007524. Pec_lyase_N.
IPR012334. Pectin_lyas_fold.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00544. Pec_lyase_C. 1 hit.
PF04431. Pec_lyase_N. 1 hit.
[Graphical view]
PRINTSPR00807. AMBALLERGEN.
SMARTSM00656. Amb_all. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePEL7_ARATH
AccessionPrimary (citable) accession number: Q9SRH4
Secondary accession number(s): Q8LGC8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: May 1, 2000
Last modified: November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents