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Protein

Protein disulfide isomerase-like 1-2

Gene

PDIL1-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei58NucleophileBy similarity1
Sitei59Contributes to redox potential valueBy similarity1
Sitei60Contributes to redox potential valueBy similarity1
Active sitei61NucleophileBy similarity1
Sitei126Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei402NucleophileBy similarity1
Sitei403Contributes to redox potential valueBy similarity1
Sitei404Contributes to redox potential valueBy similarity1
Active sitei405NucleophileBy similarity1
Sitei466Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

  • protein disulfide isomerase activity Source: TAIR

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • photoinhibition Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to endoplasmic reticulum stress Source: TAIR
  • response to high light intensity Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide isomerase-like 1-2 (EC:5.3.4.1)
Short name:
AtPDIL1-2
Alternative name(s):
Protein disulfide-isomerase 2
Short name:
PDI 2
Protein disulfide-isomerase 6
Short name:
AtPDI6
Gene namesi
Name:PDIL1-2
Synonyms:PDI6
Ordered Locus Names:At1g77510
ORF Names:T5M16.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G77510.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • Golgi apparatus Source: TAIR
  • plasma membrane Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000003420625 – 508Protein disulfide isomerase-like 1-2Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi38N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi58 ↔ 61Redox-activePROSITE-ProRule annotation
Glycosylationi273N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi402 ↔ 405Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9SRG3.
PRIDEiQ9SRG3.

PTM databases

iPTMnetiQ9SRG3.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

By chemically-induced ER stress response.1 Publication

Gene expression databases

GenevisibleiQ9SRG3. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G77510.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SRG3.
SMRiQ9SRG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 140Thioredoxin 1PROSITE-ProRule annotationAdd BLAST116
Domaini336 – 480Thioredoxin 2PROSITE-ProRule annotationAdd BLAST145

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi505 – 508Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000162459.
InParanoidiQ9SRG3.
KOiK09580.
OMAiDNVHDFV.
OrthoDBiEOG09360AJD.
PhylomeDBiQ9SRG3.

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SRG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFKGFACFS ILLLLSLFVS SIRSEETKEF VLTLDHSNFT ETISKHDFIV
60 70 80 90 100
VEFYAPWCGH CQKLAPEYEK AASELSSHNP PLALAKIDAS EEANKEFANE
110 120 130 140 150
YKIQGFPTLK ILRNGGKSVQ DYNGPREAEG IVTYLKKQSG PASVEIKSAD
160 170 180 190 200
SATEVVGEKN VVAVGVFPKL SGDEFDSFMA LAEKLRADYD FAHTLDAKFL
210 220 230 240 250
PRGESVEGPA VRLFKPFDEL FVDSKDFNGE ALEKFVKESS IPLVTVFDSD
260 270 280 290 300
PNNHPYVAKF FESPATKAMM FVNFTGATAE ALKSKYREVA TSNKDQSLAF
310 320 330 340 350
LVGDAESSQG AFQYFGLEES QVPLIIIQTP DNKKYLKVNV EVDQIESWFK
360 370 380 390 400
DFQDGKVAVH KKSQPIPAEN NEPVKVVVAE SLDDIVFKSG KNVLIEFYAP
410 420 430 440 450
WCGHCQKLAP ILDEVALSFQ NDPSVIIAKL DATANDIPSD TFDVKGFPTI
460 470 480 490 500
YFRSASGNVV VYEGDRTKED FINFVEKNSE KKPTSHGEES TKSEEPKKTE

ETAAKDEL
Length:508
Mass (Da):56,365
Last modified:May 1, 2000 - v1
Checksum:iB6300A31DFD2AB62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010704 Genomic DNA. Translation: AAG51673.1.
CP002684 Genomic DNA. Translation: AEE35987.1.
AK226862 mRNA. Translation: BAE98951.1.
PIRiE96804.
RefSeqiNP_177875.1. NM_106400.3.
UniGeneiAt.17801.

Genome annotation databases

EnsemblPlantsiAT1G77510.1; AT1G77510.1; AT1G77510.
GeneIDi844087.
GrameneiAT1G77510.1; AT1G77510.1; AT1G77510.
KEGGiath:AT1G77510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010704 Genomic DNA. Translation: AAG51673.1.
CP002684 Genomic DNA. Translation: AEE35987.1.
AK226862 mRNA. Translation: BAE98951.1.
PIRiE96804.
RefSeqiNP_177875.1. NM_106400.3.
UniGeneiAt.17801.

3D structure databases

ProteinModelPortaliQ9SRG3.
SMRiQ9SRG3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G77510.1.

PTM databases

iPTMnetiQ9SRG3.

Proteomic databases

PaxDbiQ9SRG3.
PRIDEiQ9SRG3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G77510.1; AT1G77510.1; AT1G77510.
GeneIDi844087.
GrameneiAT1G77510.1; AT1G77510.1; AT1G77510.
KEGGiath:AT1G77510.

Organism-specific databases

TAIRiAT1G77510.

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000162459.
InParanoidiQ9SRG3.
KOiK09580.
OMAiDNVHDFV.
OrthoDBiEOG09360AJD.
PhylomeDBiQ9SRG3.

Miscellaneous databases

PROiQ9SRG3.

Gene expression databases

GenevisibleiQ9SRG3. AT.

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDI12_ARATH
AccessioniPrimary (citable) accession number: Q9SRG3
Secondary accession number(s): Q0WV97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.