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Q9SRG3

- PDI12_ARATH

UniProt

Q9SRG3 - PDI12_ARATH

Protein

Protein disulfide isomerase-like 1-2

Gene

PDIL1-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.By similarity

    Catalytic activityi

    Catalyzes the rearrangement of -S-S- bonds in proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei58 – 581NucleophileBy similarity
    Sitei59 – 591Contributes to redox potential valueBy similarity
    Sitei60 – 601Contributes to redox potential valueBy similarity
    Active sitei61 – 611NucleophileBy similarity
    Sitei126 – 1261Lowers pKa of C-terminal Cys of first active siteBy similarity
    Active sitei402 – 4021NucleophileBy similarity
    Sitei403 – 4031Contributes to redox potential valueBy similarity
    Sitei404 – 4041Contributes to redox potential valueBy similarity
    Active sitei405 – 4051NucleophileBy similarity
    Sitei466 – 4661Lowers pKa of C-terminal Cys of second active siteBy similarity

    GO - Molecular functioni

    1. protein disulfide isomerase activity Source: TAIR

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. response to cadmium ion Source: TAIR
    3. response to endoplasmic reticulum stress Source: TAIR
    4. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciARA:AT1G77510-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein disulfide isomerase-like 1-2 (EC:5.3.4.1)
    Short name:
    AtPDIL1-2
    Alternative name(s):
    Protein disulfide-isomerase 2
    Short name:
    PDI 2
    Protein disulfide-isomerase 6
    Short name:
    AtPDI6
    Gene namesi
    Name:PDIL1-2
    Synonyms:PDI6
    Ordered Locus Names:At1g77510
    ORF Names:T5M16.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G77510.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. endoplasmic reticulum Source: TAIR
    3. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    4. Golgi apparatus Source: TAIR
    5. plasma membrane Source: TAIR
    6. vacuolar membrane Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 508484Protein disulfide isomerase-like 1-2PRO_0000034206Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi58 ↔ 61Redox-activePROSITE-ProRule annotation
    Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi402 ↔ 405Redox-activePROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9SRG3.
    PRIDEiQ9SRG3.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Inductioni

    By chemically-induced ER stress response.1 Publication

    Gene expression databases

    ArrayExpressiQ9SRG3.
    GenevestigatoriQ9SRG3.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SRG3.
    SMRiQ9SRG3. Positions 29-483.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 140116Thioredoxin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini336 – 480145Thioredoxin 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi505 – 5084Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the protein disulfide isomerase family.Curated
    Contains 2 thioredoxin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG0526.
    HOGENOMiHOG000162459.
    InParanoidiQ9SRG3.
    KOiK09580.
    OMAiSHGEEST.
    PhylomeDBiQ9SRG3.

    Family and domain databases

    Gene3Di3.40.30.10. 3 hits.
    InterProiIPR005788. Disulphide_isomerase.
    IPR005792. Prot_disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view]
    PfamiPF00085. Thioredoxin. 2 hits.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 4 hits.
    TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
    TIGR01126. pdi_dom. 2 hits.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SRG3-1 [UniParc]FASTAAdd to Basket

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    MAFKGFACFS ILLLLSLFVS SIRSEETKEF VLTLDHSNFT ETISKHDFIV    50
    VEFYAPWCGH CQKLAPEYEK AASELSSHNP PLALAKIDAS EEANKEFANE 100
    YKIQGFPTLK ILRNGGKSVQ DYNGPREAEG IVTYLKKQSG PASVEIKSAD 150
    SATEVVGEKN VVAVGVFPKL SGDEFDSFMA LAEKLRADYD FAHTLDAKFL 200
    PRGESVEGPA VRLFKPFDEL FVDSKDFNGE ALEKFVKESS IPLVTVFDSD 250
    PNNHPYVAKF FESPATKAMM FVNFTGATAE ALKSKYREVA TSNKDQSLAF 300
    LVGDAESSQG AFQYFGLEES QVPLIIIQTP DNKKYLKVNV EVDQIESWFK 350
    DFQDGKVAVH KKSQPIPAEN NEPVKVVVAE SLDDIVFKSG KNVLIEFYAP 400
    WCGHCQKLAP ILDEVALSFQ NDPSVIIAKL DATANDIPSD TFDVKGFPTI 450
    YFRSASGNVV VYEGDRTKED FINFVEKNSE KKPTSHGEES TKSEEPKKTE 500
    ETAAKDEL 508
    Length:508
    Mass (Da):56,365
    Last modified:May 1, 2000 - v1
    Checksum:iB6300A31DFD2AB62
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC010704 Genomic DNA. Translation: AAG51673.1.
    CP002684 Genomic DNA. Translation: AEE35987.1.
    AK226862 mRNA. Translation: BAE98951.1.
    PIRiE96804.
    RefSeqiNP_177875.1. NM_106400.2.
    UniGeneiAt.17801.

    Genome annotation databases

    EnsemblPlantsiAT1G77510.1; AT1G77510.1; AT1G77510.
    GeneIDi844087.
    KEGGiath:AT1G77510.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC010704 Genomic DNA. Translation: AAG51673.1 .
    CP002684 Genomic DNA. Translation: AEE35987.1 .
    AK226862 mRNA. Translation: BAE98951.1 .
    PIRi E96804.
    RefSeqi NP_177875.1. NM_106400.2.
    UniGenei At.17801.

    3D structure databases

    ProteinModelPortali Q9SRG3.
    SMRi Q9SRG3. Positions 29-483.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9SRG3.
    PRIDEi Q9SRG3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G77510.1 ; AT1G77510.1 ; AT1G77510 .
    GeneIDi 844087.
    KEGGi ath:AT1G77510.

    Organism-specific databases

    TAIRi AT1G77510.

    Phylogenomic databases

    eggNOGi COG0526.
    HOGENOMi HOG000162459.
    InParanoidi Q9SRG3.
    KOi K09580.
    OMAi SHGEEST.
    PhylomeDBi Q9SRG3.

    Enzyme and pathway databases

    BioCyci ARA:AT1G77510-MONOMER.

    Miscellaneous databases

    PROi Q9SRG3.

    Gene expression databases

    ArrayExpressi Q9SRG3.
    Genevestigatori Q9SRG3.

    Family and domain databases

    Gene3Di 3.40.30.10. 3 hits.
    InterProi IPR005788. Disulphide_isomerase.
    IPR005792. Prot_disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view ]
    Pfami PF00085. Thioredoxin. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 4 hits.
    TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
    TIGR01126. pdi_dom. 2 hits.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins."
      Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S.
      Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana."
      Lu D.-P., Christopher D.A.
      Mol. Genet. Genomics 280:199-210(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    6. "The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)."
      d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M.
      BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPDI12_ARATH
    AccessioniPrimary (citable) accession number: Q9SRG3
    Secondary accession number(s): Q0WV97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3