Reviewed, UniProtKB/Swiss-Prot Q9SR59 (MDAR1_ARATH)

Names and origin

Protein names

Recommended name:
    Probable monodehydroascorbate reductase, cytoplasmic isoform 1
      Short name=MDAR 1
    EC=1.6.5.4

Gene names

Ordered Locus Names:	At3g09940
ORF Names:	T22K18.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process By similarity.
Catalytic activity	NADH + 2 monodehydroascorbate = NAD(+) + 2 ascorbate.
Cofactor	FAD By similarity.
Subcellular location	CytoplasmPotential.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Redox-active center
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	monodehydroascorbate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Notes: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SR59-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Probable monodehydroascorbate reductase, cytoplasmic isoform 1		PRO_0000209136

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7552A50B0C14715F	FASTA	441	48,363
	10 20 30 40 50 60 MAEEKSYKYV IIGGGVAGGY AAREFSNQGL KPGELAIISK EPVPPFERPE LTKVYIDLEV 70 80 90 100 110 120 NPTLANIYVC AGTGEAKQYP NWYKEKGIDL IVGTEIVKAD LASKTLVSDD GKIYKYQTLL 130 140 150 160 170 180 IATGSTNIRL SEIGVQEADV KNIFYLREIE DSDELALAME LYVQRGKAVI IGGGFLGLEI 190 200 210 220 230 240 SSALRANNHE VTMVFPEPWL VHRFFTAEIA SFYESYYANK GIKIIKGTVA TGFSTNSDGE 250 260 270 280 290 300 VTEVKLEDGR TLEANIVVAG VGARPATSLF KGQLEEEKGG IKTDGFFKTS VPDVYALGDV 310 320 330 340 350 360 ATFPMKMYGG TRRVEHADNA RKSAAQAVKA IKAGEEGKTI PDYDYLPYFY SRFFKLSWEF 370 380 390 400 410 420 YGENVGESVL FGDNDPKSPK PKFGTYWVKD GKVVGVFLEG GTQEEHKAIA KVARAQPSVE 430 440 SLDVLSEEGL SFATKFYSTS L

References

[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC010927 Genomic DNA. Translation: AAF04429.1. AY093765 mRNA. Translation: AAM10387.1. BT001054 mRNA. Translation: AAN46808.1. AY084556 mRNA. Translation: AAM61123.1.
RefSeq	NP_566361.1.
UniGene	At.40014
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	820155.
GenomeReviews	Gene locus AT3G09940 in contig BA000014_GR.
NMPDR	fig|3702.1.peg.13016.
Organism-specific databases
TAIR	At3g09940.
Gene expression databases
ArrayExpress	Q9SR59.
GermOnline	AT3G09940. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

MDAR1_ARATH

Accession

Primary (citable) accession number: Q9SR59

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	May 1, 2000
Last modified:	November 4, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)