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Protein

Urease

Gene

URE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism.1 Publication

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.

Cofactori

Ni cationBy similarityNote: Binds 2 nickel ions per subunit.By similarity

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).
Proteins known to be involved in this subpathway in this organism are:
  1. Urease (URE)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi405 – 4051Nickel 1; via tele nitrogenBy similarity
Metal bindingi407 – 4071Nickel 1; via tele nitrogenBy similarity
Metal bindingi488 – 4881Nickel 1; via carbamate groupBy similarity
Metal bindingi488 – 4881Nickel 2; via carbamate groupBy similarity
Binding sitei490 – 4901SubstrateBy similarity
Metal bindingi517 – 5171Nickel 2; via pros nitrogenBy similarity
Metal bindingi543 – 5431Nickel 2; via tele nitrogenBy similarity
Active sitei591 – 5911Proton donorBy similarity
Metal bindingi631 – 6311Nickel 1By similarity

GO - Molecular functioni

  • nickel cation binding Source: InterPro
  • urease activity Source: TAIR

GO - Biological processi

  • nitrogen compound metabolic process Source: TAIR
  • urea catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciARA:AT1G67550-MONOMER.
UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease (EC:3.5.1.5)
Alternative name(s):
Urea amidohydrolase
Gene namesi
Name:URE
Ordered Locus Names:At1g67550
ORF Names:F12B7.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G67550.

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants cannot grow on medium with urea as the sole source of nitrogen.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 838838UreasePRO_0000424246Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei488 – 4881N6-carboxylysineBy similarity

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.By similarity

Proteomic databases

PaxDbiQ9SR52.
PRIDEiQ9SR52.

PTM databases

iPTMnetiQ9SR52.

Expressioni

Gene expression databases

GenevisibleiQ9SR52. AT.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G67550.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SR52.
SMRiQ9SR52. Positions 1-838.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini400 – 838439UreaseAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.Curated
Contains 1 urease domain.Curated

Phylogenomic databases

eggNOGiENOG410IGM6. Eukaryota.
ENOG410JX8F. Eukaryota.
COG0804. LUCA.
COG0831. LUCA.
COG0832. LUCA.
HOGENOMiHOG000075064.
InParanoidiQ9SR52.
KOiK01427.
OMAiLPINMGF.
OrthoDBiEOG093601ZL.
PhylomeDBiQ9SR52.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
cd00407. Urease_beta. 1 hit.
cd00390. Urease_gamma. 1 hit.
Gene3Di2.10.150.10. 1 hit.
2.30.40.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
MF_01954. Urease_beta. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR008221. Urease.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR002019. Urease_beta.
IPR002026. Urease_gamma/gamma-beta_su.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
PF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001222. Urease. 1 hit.
PRINTSiPR01752. UREASE.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51278. SSF51278. 1 hit.
SSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
SSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
TIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SR52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLPREIEK LELHQAGFLA QKRLARGIRL NYTEAVALIA TQILEFIRDG
60 70 80 90 100
DKSVAELMDI GRQLLGRRQV LPAVLHLLYT VQVEGTFRDG TKLVTVHEPI
110 120 130 140 150
SLENGNLELA LHGSFLPVPS LDKFPEVHEG VIIPGDMKYG DGSIIINHGR
160 170 180 190 200
KAVVLKVVNT GDRPVQVGSH YHFIEVNPLL VFDRRKALGM RLNIPAGTAV
210 220 230 240 250
RFEPGERKSV VLVNIGGNKV IRGGNGIVDG LVDDVNWTVL METMERRGFK
260 270 280 290 300
HLEDIDASEG IAGEDPRFTT MISREKYANM YGPTTGDKLR LGDTNLYARI
310 320 330 340 350
EKDYTVYGDE CVFGGGKVLR EGMGQGIEQA EALSLDTVIT NSVIIDYSGI
360 370 380 390 400
YKADIGIKNG HIVGIGKAGN PDTMHGVQNN MLIGNKTEVI AGEGMIVTAG
410 420 430 440 450
AIDCHVHFIC PQLVYEAVSS GITTMVGGGT GPAYGTRATT CTPSPFDMKL
460 470 480 490 500
MLQSTDSLPL NFGFTGKGNT AKPLELRHIV EAGAMGLKLH EDWGTTPAAI
510 520 530 540 550
DNCLAVAEEY DIQVNIHTDT LNESGFVEHT INAFRGRTIH TYHSEGAGGG
560 570 580 590 600
HAPDIIRVCG VKNVLPSSTN PTRPYTKNTV DEHLDMLMVC HHLDKNIPED
610 620 630 640 650
VAFAESRIRA ETIAAEDILH DMGAISIISS DSQAMGRIGE VISRTWQTAD
660 670 680 690 700
KMKAQRGAID PNMADDDNSR IKRYIAKYTI NPAIANGFAD LIGSVEVKKL
710 720 730 740 750
ADLVIWQPAF FGAKPEMIIK GGNIAWANMG DANASIPTPE PVISRPMFGA
760 770 780 790 800
FGKAGSENSV AFVSKAALRK GVKELYGLKK RVVAVSNVRQ LTKLDMKLND
810 820 830
ALPEITVDPE TYVVTANGEV LTCAPADSVP LSRNYFLF
Length:838
Mass (Da):91,024
Last modified:May 1, 2000 - v1
Checksum:iE60C9DD1B07FE754
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011020 Genomic DNA. Translation: AAG52306.1.
CP002684 Genomic DNA. Translation: AEE34663.1.
PIRiA96699.
RefSeqiNP_176922.1. NM_105422.3.
UniGeneiAt.35634.

Genome annotation databases

EnsemblPlantsiAT1G67550.1; AT1G67550.1; AT1G67550.
GeneIDi843076.
GrameneiAT1G67550.1; AT1G67550.1; AT1G67550.
KEGGiath:AT1G67550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011020 Genomic DNA. Translation: AAG52306.1.
CP002684 Genomic DNA. Translation: AEE34663.1.
PIRiA96699.
RefSeqiNP_176922.1. NM_105422.3.
UniGeneiAt.35634.

3D structure databases

ProteinModelPortaliQ9SR52.
SMRiQ9SR52. Positions 1-838.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G67550.1.

Protein family/group databases

MEROPSiM38.982.

PTM databases

iPTMnetiQ9SR52.

Proteomic databases

PaxDbiQ9SR52.
PRIDEiQ9SR52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G67550.1; AT1G67550.1; AT1G67550.
GeneIDi843076.
GrameneiAT1G67550.1; AT1G67550.1; AT1G67550.
KEGGiath:AT1G67550.

Organism-specific databases

TAIRiAT1G67550.

Phylogenomic databases

eggNOGiENOG410IGM6. Eukaryota.
ENOG410JX8F. Eukaryota.
COG0804. LUCA.
COG0831. LUCA.
COG0832. LUCA.
HOGENOMiHOG000075064.
InParanoidiQ9SR52.
KOiK01427.
OMAiLPINMGF.
OrthoDBiEOG093601ZL.
PhylomeDBiQ9SR52.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciARA:AT1G67550-MONOMER.

Miscellaneous databases

PROiQ9SR52.

Gene expression databases

GenevisibleiQ9SR52. AT.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
cd00407. Urease_beta. 1 hit.
cd00390. Urease_gamma. 1 hit.
Gene3Di2.10.150.10. 1 hit.
2.30.40.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
MF_01954. Urease_beta. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR008221. Urease.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR002019. Urease_beta.
IPR002026. Urease_gamma/gamma-beta_su.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
PF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001222. Urease. 1 hit.
PRINTSiPR01752. UREASE.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51278. SSF51278. 1 hit.
SSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
SSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
TIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUREA_ARATH
AccessioniPrimary (citable) accession number: Q9SR52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.