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Protein

Urease

Gene

URE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism.1 Publication

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.

Cofactori

Ni cationBy similarityNote: Binds 2 nickel ions per subunit.By similarity

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).
Proteins known to be involved in this subpathway in this organism are:
  1. Urease (URE), Urease (AXX17_At1g61490)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi405Nickel 1; via tele nitrogenBy similarity1
Metal bindingi407Nickel 1; via tele nitrogenBy similarity1
Metal bindingi488Nickel 1; via carbamate groupBy similarity1
Metal bindingi488Nickel 2; via carbamate groupBy similarity1
Binding sitei490SubstrateBy similarity1
Metal bindingi517Nickel 2; via pros nitrogenBy similarity1
Metal bindingi543Nickel 2; via tele nitrogenBy similarity1
Active sitei591Proton donorBy similarity1
Metal bindingi631Nickel 1By similarity1

GO - Molecular functioni

  • nickel cation binding Source: InterPro
  • urease activity Source: TAIR

GO - Biological processi

  • nitrogen compound metabolic process Source: TAIR
  • urea catabolic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Nickel

Enzyme and pathway databases

BioCyciARA:AT1G67550-MONOMER
UniPathwayiUPA00258; UER00370

Protein family/group databases

MEROPSiM38.982

Names & Taxonomyi

Protein namesi
Recommended name:
Urease (EC:3.5.1.5)
Alternative name(s):
Urea amidohydrolase
Gene namesi
Name:URE
Ordered Locus Names:At1g67550
ORF Names:F12B7.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G67550
TAIRilocus:2008798 AT1G67550

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants cannot grow on medium with urea as the sole source of nitrogen.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004242461 – 838UreaseAdd BLAST838

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei488N6-carboxylysineBy similarity1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.By similarity

Proteomic databases

PaxDbiQ9SR52
PRIDEiQ9SR52

PTM databases

iPTMnetiQ9SR52

Expressioni

Gene expression databases

ExpressionAtlasiQ9SR52 baseline and differential
GenevisibleiQ9SR52 AT

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G67550.1

Structurei

3D structure databases

ProteinModelPortaliQ9SR52
SMRiQ9SR52
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini400 – 838UreaseAdd BLAST439

Sequence similaritiesi

In the C-terminal section; belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.Curated

Phylogenomic databases

eggNOGiENOG410IGM6 Eukaryota
ENOG410JX8F Eukaryota
COG0804 LUCA
COG0831 LUCA
COG0832 LUCA
HOGENOMiHOG000075064
InParanoidiQ9SR52
KOiK01427
OMAiGFDSHIH
OrthoDBiEOG093601ZL
PhylomeDBiQ9SR52

Family and domain databases

CDDicd00375 Urease_alpha, 1 hit
cd00407 Urease_beta, 1 hit
cd00390 Urease_gamma, 1 hit
Gene3Di2.10.150.10, 1 hit
2.30.40.10, 2 hits
3.30.280.10, 1 hit
HAMAPiMF_01953 Urease_alpha, 1 hit
MF_01954 Urease_beta, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
IPR008221 Urease
IPR011612 Urease_alpha_N_dom
IPR017950 Urease_AS
IPR005848 Urease_asu
IPR017951 Urease_asu_c
IPR002019 Urease_beta
IPR036461 Urease_betasu_sf
IPR002026 Urease_gamma/gamma-beta_su
IPR036463 Urease_gamma_sf
IPR029754 Urease_Ni-bd
PANTHERiPTHR43440 PTHR43440, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
PF00449 Urease_alpha, 1 hit
PF00699 Urease_beta, 1 hit
PF00547 Urease_gamma, 1 hit
PIRSFiPIRSF001222 Urease, 1 hit
PRINTSiPR01752 UREASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002319 Urease_gamma_reg, 1 hit
SUPFAMiSSF51278 SSF51278, 1 hit
SSF51338 SSF51338, 2 hits
SSF51556 SSF51556, 2 hits
SSF54111 SSF54111, 1 hit
TIGRFAMsiTIGR01792 urease_alph, 1 hit
TIGR00192 urease_beta, 1 hit
TIGR00193 urease_gam, 1 hit
PROSITEiView protein in PROSITE
PS01120 UREASE_1, 1 hit
PS00145 UREASE_2, 1 hit
PS51368 UREASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SR52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLPREIEK LELHQAGFLA QKRLARGIRL NYTEAVALIA TQILEFIRDG
60 70 80 90 100
DKSVAELMDI GRQLLGRRQV LPAVLHLLYT VQVEGTFRDG TKLVTVHEPI
110 120 130 140 150
SLENGNLELA LHGSFLPVPS LDKFPEVHEG VIIPGDMKYG DGSIIINHGR
160 170 180 190 200
KAVVLKVVNT GDRPVQVGSH YHFIEVNPLL VFDRRKALGM RLNIPAGTAV
210 220 230 240 250
RFEPGERKSV VLVNIGGNKV IRGGNGIVDG LVDDVNWTVL METMERRGFK
260 270 280 290 300
HLEDIDASEG IAGEDPRFTT MISREKYANM YGPTTGDKLR LGDTNLYARI
310 320 330 340 350
EKDYTVYGDE CVFGGGKVLR EGMGQGIEQA EALSLDTVIT NSVIIDYSGI
360 370 380 390 400
YKADIGIKNG HIVGIGKAGN PDTMHGVQNN MLIGNKTEVI AGEGMIVTAG
410 420 430 440 450
AIDCHVHFIC PQLVYEAVSS GITTMVGGGT GPAYGTRATT CTPSPFDMKL
460 470 480 490 500
MLQSTDSLPL NFGFTGKGNT AKPLELRHIV EAGAMGLKLH EDWGTTPAAI
510 520 530 540 550
DNCLAVAEEY DIQVNIHTDT LNESGFVEHT INAFRGRTIH TYHSEGAGGG
560 570 580 590 600
HAPDIIRVCG VKNVLPSSTN PTRPYTKNTV DEHLDMLMVC HHLDKNIPED
610 620 630 640 650
VAFAESRIRA ETIAAEDILH DMGAISIISS DSQAMGRIGE VISRTWQTAD
660 670 680 690 700
KMKAQRGAID PNMADDDNSR IKRYIAKYTI NPAIANGFAD LIGSVEVKKL
710 720 730 740 750
ADLVIWQPAF FGAKPEMIIK GGNIAWANMG DANASIPTPE PVISRPMFGA
760 770 780 790 800
FGKAGSENSV AFVSKAALRK GVKELYGLKK RVVAVSNVRQ LTKLDMKLND
810 820 830
ALPEITVDPE TYVVTANGEV LTCAPADSVP LSRNYFLF
Length:838
Mass (Da):91,024
Last modified:May 1, 2000 - v1
Checksum:iE60C9DD1B07FE754
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011020 Genomic DNA Translation: AAG52306.1
CP002684 Genomic DNA Translation: AEE34663.1
PIRiA96699
RefSeqiNP_176922.1, NM_105422.4
UniGeneiAt.35634

Genome annotation databases

EnsemblPlantsiAT1G67550.1; AT1G67550.1; AT1G67550
GeneIDi843076
GrameneiAT1G67550.1; AT1G67550.1; AT1G67550
KEGGiath:AT1G67550

Similar proteinsi

Entry informationi

Entry nameiUREA_ARATH
AccessioniPrimary (citable) accession number: Q9SR52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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