Reviewed, UniProtKB/Swiss-Prot Q9SR43 (PFBS_ARATH)

Names and origin

Protein names

Recommended name:
    Phytochromobilin:ferredoxin oxidoreductase, chloroplastic
    EC=1.3.7.4
Alternative name(s):
    Phytochromobilin synthase
    PFB synthase
    PPhiB synthase

Gene names

Name:	HY2
Ordered Locus Names:	At3g09150
ORF Names:	F3L24.1, MZB10.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the two-electron reduction of biliverdin IX-alpha to the tetrapyrrole chromophore phytochromobilin (PPhiB).
Catalytic activity	(3Z)-phytochromobilin + oxidized ferredoxin = biliverdin IX-alpha + reduced ferredoxin.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the HY2 family.
Sequence caution	The sequence AAD56331.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chloroplast-nucleus signaling pathway Inferred from mutant phenotype. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW phytochromobilin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Ref.1 Inferred from direct assay. Source: TAIR
Molecular function	cobalt ion binding Inferred from electronic annotation. Source: InterPro phytochromobilin:ferredoxin oxidoreductase activity Ref.1 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9SR43-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9SR43-2)

The sequence of this isoform differs from the canonical sequence as follows:
     75-76: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 329		Phytochromobilin:ferredoxin oxidoreductase, chloroplastic		PRO_0000013609
Natural variations
	Alternative sequence	75 – 76	2	Missing in isoform 2.		VSP_008970
Experimental info
	Sequence conflict	39	1	K → N in AAM67180. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 98727CD1778D973C	FASTA	329	38,130
	10 20 30 40 50 60 MALSMEFGFS IGSCFKAPNP PVLISASPNK INFTLRRRKK RFLLRVSAVS YKEFAESALE 70 80 90 100 110 120 ETRKRIVLEP SHLQEKYSSM TGLDGKTELQ MLAFKSSKIR LLRSMAIENE TMQVFDFAGF 130 140 150 160 170 180 MEPEYDTPIF CANFFTSTNV NIVVLDLNPL HQLTDQTDYQ DKYYNKIMSI YHKYAETFPW 190 200 210 220 230 240 GGKLTGESIK FFSPLVMWTR FSSSKEKHKA LFSAFLEYYQ AWLEMTIQVR EEMEPSHVRA 250 260 270 280 290 300 NCEAQHKYLT WRAQKDPGHG LLKRLVGEAK AKELLRDFLF NGVDELGTKT FIDYFPEYQT 310 320 EDGTVSDKRS IIGKSYETRP WDLTGQFIG
	Isoform 2. Checksum: B37969DD116D647E	FASTA	327	37,872
	10 20 30 40 50 60 MALSMEFGFS IGSCFKAPNP PVLISASPNK INFTLRRRKK RFLLRVSAVS YKEFAESALE 70 80 90 100 110 120 ETRKRIVLEP SHLQYSSMTG LDGKTELQML AFKSSKIRLL RSMAIENETM QVFDFAGFME 130 140 150 160 170 180 PEYDTPIFCA NFFTSTNVNI VVLDLNPLHQ LTDQTDYQDK YYNKIMSIYH KYAETFPWGG 190 200 210 220 230 240 KLTGESIKFF SPLVMWTRFS SSKEKHKALF SAFLEYYQAW LEMTIQVREE MEPSHVRANC 250 260 270 280 290 300 EAQHKYLTWR AQKDPGHGLL KRLVGEAKAK ELLRDFLFNG VDELGTKTFI DYFPEYQTED 310 320 GTVSDKRSII GKSYETRPWD LTGQFIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis HY2 gene encodes phytochromobilin synthase, a ferredoxin-dependent biliverdin reductase." Kohchi T., Mukougawa K., Frankenberg N., Masuda M., Yokota A., Lagarias J.C. Plant Cell 13:425-436(2001) [PubMed: 11226195] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB045112 mRNA. Translation: BAB33374.1. AC011436 Genomic DNA. Translation: AAF14017.1. AC009326 Genomic DNA. Translation: AAD56331.1. Sequence problems. AY099706 mRNA. Translation: AAM20557.1. AY128900 mRNA. Translation: AAM91300.1. AY088874 mRNA. Translation: AAM67180.1. Different initiation.
IPI	IPI00518705. IPI00531995.
RefSeq	NP_566344.2. NP_850996.1.
UniGene	At.10486
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9SR43.
Genome annotation databases
GeneID	820069.
GenomeReviews	Gene locus AT3G09150 in contig BA000014_GR.
KEGG	ath:AT3G09150.
NMPDR	fig|3702.1.peg.12927.
Organism-specific databases
TAIR	At3g09150.
Phylogenomic databases
OMA	Q9SR43. FCANFFT.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13949.
BRENDA	1.3.7.4. 302.
Gene expression databases
ArrayExpress	Q9SR43.
Family and domain databases
InterPro	IPR009249. Ferredoxin-dep_bilin_Rdtase. [Graphical view]
Pfam	PF05996. Fe_bilin_red. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PFBS_ARATH

Accession

Primary (citable) accession number: Q9SR43
Secondary accession number(s): Q8L8Q6, Q9SS72

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)