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Protein

NAC domain containing protein 50

Gene

NAC050

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor that binds to the motif 5'-(C/T)A(C/A)G-3' in the promoter of target genes (PubMed:25578968). Binds also to the 5'-CTTGNNNNNCAAG-3' consensus sequence in chromatin (PubMed:26617990). Can bind to the mitochondrial dysfunction motif (MDM) present in the upstream regions of mitochondrial dysfunction stimulon (MDS) genes involved in mitochondrial retrograde regulation (MRR) (PubMed:24045019). Together with NAC051/NAC052 and JMJ14, regulates gene expression and flowering time by associating with the histone demethylase JMJ14, probably by the promotion of RNA-mediated gene silencing (PubMed:25578968, PubMed:26617990).3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi126 – 184PROSITE-ProRule annotationAdd BLAST59

GO - Molecular functioni

  • sequence-specific DNA binding Source: UniProtKB
  • transcription regulatory region sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • flower development Source: UniProtKB-KW
  • histone H3-K4 demethylation Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • photoperiodism, flowering Source: UniProtKB
  • regulation of gene silencing by RNA Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processFlowering, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NAC domain containing protein 501 Publication
Short name:
ANAC0501 Publication
Gene namesi
Name:NAC0501 Publication
Synonyms:NAC501 Publication
Ordered Locus Names:At3g10480Imported
ORF Names:F13M14.24Imported, F18K10.5Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G10480

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

The double mutant nac050 nac052 exhibits early flowering and increased H3K4 methylation on specific genes, thus leading to their derepression (PubMed:25578968). Impaired RNA-mediated gene silencing (PubMed:26617990).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004421941 – 447NAC domain containing protein 50Add BLAST447

PTM databases

iPTMnetiQ9SQX9

Expressioni

Tissue specificityi

Mostly expressed in floral organs, and, at low levels, in other organs.1 Publication

Gene expression databases

ExpressionAtlasiQ9SQX9 baseline and differential

Interactioni

Subunit structurei

Interacts with JMJ14 and NAC052.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiQ9SQX9, 17 interactors

Structurei

3D structure databases

SMRiQ9SQX9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 178NACPROSITE-ProRule annotationAdd BLAST152

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili392 – 447Sequence analysisAdd BLAST56

Domaini

The NAC domain includes a DNA binding domain and a dimerization domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000029101

Family and domain databases

Gene3Di3.30.310.150, 1 hit
InterProiView protein in InterPro
IPR003441 NAC-dom
IPR036093 NAC_dom_sf
PfamiView protein in Pfam
PF02365 NAM, 1 hit
SUPFAMiSSF101941 SSF101941, 1 hit
PROSITEiView protein in PROSITE
PS51005 NAC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SQX9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRESLAVVS SPPSATAPST AVSATSLAPG FRFHPTDEEL VSYYLKRKVL
60 70 80 90 100
GKPVRFDAIG EVDIYKHEPW DLAVFSKLKT RDQEWYFFSA LDKKYGNGAR
110 120 130 140 150
MNRATNKGYW KATGKDREIR RDIQLLGMKK TLVFHSGRAP DGLRTNWVMH
160 170 180 190 200
EYRLVEYETE TNGSLLQDAY VLCRVFHKNN IGPPSGNRYA PFMEEEWADG
210 220 230 240 250
GGALIPGIDV RVRVEALPQA NGNNQMDQEM HSASKDLINI NELPRDATPM
260 270 280 290 300
DIEPNQQNHH ESAFKPQESN NHSGYEEDED TLKREHAEED ERPPSLCILN
310 320 330 340 350
KEAPLPLLQY KRRRQNESNN NSSRNTQDHC SSTITTVDNT TTLISSSAAA
360 370 380 390 400
ATNTAISALL EFSLMGISDK KENQQKEETS PPSPIASPEE KVNDLQKEVH
410 420 430 440
QMSVERETFK LEMMSAEAMI SILQSRIDAL RQENEELKKK NASGQAS
Length:447
Mass (Da):50,355
Last modified:May 1, 2000 - v1
Checksum:i9D0527D228CAD3B1
GO
Isoform 2 (identifier: Q9SQX9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     167-167: Missing.

Note: Derived from EST data. No experimental confirmation available.Imported
Show »
Length:446
Mass (Da):50,227
Checksum:i979A83C2536CCCFD
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_059198167Missing in isoform 2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011560 Genomic DNA Translation: AAG51394.1
AC013428 Genomic DNA Translation: AAF76349.1
CP002686 Genomic DNA Translation: AEE74912.1
CP002686 Genomic DNA Translation: AEE74913.1
AY059795 mRNA Translation: AAL24143.1
AY091262 mRNA Translation: AAM14201.1
RefSeqiNP_566374.1, NM_111883.5 [Q9SQX9-1]
NP_974272.1, NM_202543.2 [Q9SQX9-2]
UniGeneiAt.20114

Genome annotation databases

EnsemblPlantsiAT3G10480.1; AT3G10480.1; AT3G10480 [Q9SQX9-1]
AT3G10480.2; AT3G10480.2; AT3G10480 [Q9SQX9-2]
GeneIDi820212
GrameneiAT3G10480.1; AT3G10480.1; AT3G10480 [Q9SQX9-1]
AT3G10480.2; AT3G10480.2; AT3G10480 [Q9SQX9-2]
KEGGiath:AT3G10480

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNAC50_ARATH
AccessioniPrimary (citable) accession number: Q9SQX9
Secondary accession number(s): F4J3S8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2017
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health