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Q9SQG2

- APY1_ARATH

UniProt

Q9SQG2 - APY1_ARATH

Protein

Apyrase 1

Gene

APY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Substrate preference is ATP > ADP. Functions with APY2 to reduce extracellular ATP level which is essential for pollen germination and normal plant development. Plays a role in the regulation of stomatal function by modulating extracellular ATP levels in guard cells.5 Publications

    Catalytic activityi

    A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

    Cofactori

    Calcium.1 Publication

    Kineticsi

    1. KM=30 µM for ATP1 Publication

    Vmax=26 µmol/min/mg enzyme1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei194 – 1941Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 8211ATP-bindingCuratedAdd
    BLAST
    Nucleotide bindingi218 – 22811ATP-bindingCuratedAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. guanosine-diphosphatase activity Source: TAIR
    3. inosine-diphosphatase activity Source: TAIR
    4. nucleoside-diphosphatase activity Source: TAIR
    5. uridine-diphosphatase activity Source: TAIR

    GO - Biological processi

    1. pollen germination Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Calcium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G04080-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apyrase 1 (EC:3.6.1.5)
    Short name:
    AtAPY1
    Alternative name(s):
    ATP-diphosphatase
    ATP-diphosphohydrolase
    Adenosine diphosphatase
    Short name:
    ADPase
    NTPDase
    Nucleoside triphosphate diphosphohydrolase 1
    Gene namesi
    Name:APY1
    Ordered Locus Names:At3g04080
    ORF Names:T6K12.30
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G04080.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication. Membrane 1 Publication; Single-pass type II membrane protein 1 Publication
    Note: As cell membrane protein, the functional domain could be at the extracellular side.

    GO - Cellular componenti

    1. endosome Source: TAIR
    2. Golgi apparatus Source: TAIR
    3. Golgi membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: TAIR
    5. trans-Golgi network Source: TAIR

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions. Apy1 and apy2 double mutant displays developmental defects including the lack of functional root and shoot meristems, and morphogenetic and patterning abnormalities of the cotyledons. Double mutant exhibits a complete inhibition of pollen germination.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 471471Apyrase 1PRO_0000419905Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9SQG2.
    PRIDEiQ9SQG2.

    Expressioni

    Tissue specificityi

    Expressed in roots, root hairs, root cap, leaves, stems, trichomes, phloem throughout the plant, guard cells, filaments of young stamens, stipules, papillae of stigmas, pollen, pollen tubes and the abscission zone of siliques.4 Publications

    Inductioni

    By hypertonic stress.1 Publication

    Gene expression databases

    GenevestigatoriQ9SQG2.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G04080.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SQG2.
    SMRiQ9SQG2. Positions 70-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2121CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini43 – 471429LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei22 – 4221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi350 – 3556Poly-Gly

    Sequence similaritiesi

    Belongs to the GDA1/CD39 NTPase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5371.
    HOGENOMiHOG000220904.
    InParanoidiQ9SQG2.
    KOiK14641.
    OMAiECSAGIS.
    PhylomeDBiQ9SQG2.

    Family and domain databases

    InterProiIPR000407. GDA1_CD39_NTPase.
    [Graphical view]
    PANTHERiPTHR11782. PTHR11782. 1 hit.
    PfamiPF01150. GDA1_CD39. 1 hit.
    [Graphical view]
    PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SQG2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAKRAIGRH ESLADKVHRH RGLLLVISIP IVLIALVLLL MPGTSTSVSV    50
    IEYTMKNHEG GSNSRGPKNY AVIFDAGSSG SRVHVYCFDQ NLDLVPLENE 100
    LELFLQLKPG LSAYPNDPRQ SANSLVTLLD KAEASVPREL RPKTPVRVGA 150
    TAGLRALGHQ ASENILQAVR ELLKGRSRLK TEANAVTVLD GTQEGSYQWV 200
    TINYLLRTLG KPYSDTVGVV DLGGGSVQMA YAIPEEDAAT APKPVEGEDS 250
    YVREMYLKGR KYFLYVHSYL HYGLLAARAE ILKVSEDSNN PCIATGYAGT 300
    YKYGGKAFKA AASPSGASLD ECRRVAINAL KVNNSLCTHM KCTFGGVWNG 350
    GGGGGQKKMF VASFFFDRAA EAGFVDPNQP VAEVRPLDFE KAANKACNMR 400
    MEEGKSKFPR VEEDNLPYLC LDLVYQYTLL VDGFGLKPSQ TITLVKKVKY 450
    GDYAVEAAWP LGSAIEAVSS P 471
    Length:471
    Mass (Da):51,194
    Last modified:May 1, 2000 - v1
    Checksum:i6EF17A73207ECDA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093604 mRNA. Translation: AAF00071.1.
    JQ937231 mRNA. Translation: AFI41199.1.
    AC016829 Genomic DNA. Translation: AAF26805.1.
    CP002686 Genomic DNA. Translation: AEE74035.1.
    BT029157 mRNA. Translation: ABJ17092.1.
    RefSeqiNP_187058.1. NM_111279.4.
    UniGeneiAt.16940.

    Genome annotation databases

    EnsemblPlantsiAT3G04080.1; AT3G04080.1; AT3G04080.
    GeneIDi819563.
    KEGGiath:AT3G04080.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093604 mRNA. Translation: AAF00071.1 .
    JQ937231 mRNA. Translation: AFI41199.1 .
    AC016829 Genomic DNA. Translation: AAF26805.1 .
    CP002686 Genomic DNA. Translation: AEE74035.1 .
    BT029157 mRNA. Translation: ABJ17092.1 .
    RefSeqi NP_187058.1. NM_111279.4.
    UniGenei At.16940.

    3D structure databases

    ProteinModelPortali Q9SQG2.
    SMRi Q9SQG2. Positions 70-462.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G04080.1-P.

    Proteomic databases

    PaxDbi Q9SQG2.
    PRIDEi Q9SQG2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G04080.1 ; AT3G04080.1 ; AT3G04080 .
    GeneIDi 819563.
    KEGGi ath:AT3G04080.

    Organism-specific databases

    TAIRi AT3G04080.

    Phylogenomic databases

    eggNOGi COG5371.
    HOGENOMi HOG000220904.
    InParanoidi Q9SQG2.
    KOi K14641.
    OMAi ECSAGIS.
    PhylomeDBi Q9SQG2.

    Enzyme and pathway databases

    BioCyci ARA:AT3G04080-MONOMER.

    Miscellaneous databases

    PROi Q9SQG2.

    Gene expression databases

    Genevestigatori Q9SQG2.

    Family and domain databases

    InterProi IPR000407. GDA1_CD39_NTPase.
    [Graphical view ]
    PANTHERi PTHR11782. PTHR11782. 1 hit.
    Pfami PF01150. GDA1_CD39. 1 hit.
    [Graphical view ]
    PROSITEi PS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular and biochemical comparison of two different apyrases from Arabidopsis thaliana."
      Steinebrunner I.A., Jeter C.R., Song C., Roux S.J.
      Plant Physiol. Biochem. 38:913-922(2000)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], COFACTOR, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    2. "Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis."
      Parsons H.T., Christiansen K., Knierim B., Carroll A., Ito J., Batth T.S., Smith-Moritz A.M., Morrison S., McInerney P., Hadi M.Z., Auer M., Mukhopadhyay A., Petzold C.J., Scheller H.V., Loque D., Heazlewood J.L.
      Plant Physiol. 159:12-26(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
      Strain: cv. Landsberg erecta.
    3. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Arabidopsis ORF Clone."
      Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Disruption of apyrases inhibits pollen germination in Arabidopsis."
      Steinebrunner I., Wu J., Sun Y., Corbett A., Roux S.J.
      Plant Physiol. 131:1638-1647(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: cv. Wassilewskija.
    7. "Developmental defects and seedling lethality in apyrase AtAPY1 and AtAPY2 double knockout mutants."
      Wolf C., Hennig M., Romanovicz D., Steinebrunner I.
      Plant Mol. Biol. 64:657-672(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: cv. Wassilewskija.
    8. "Apyrases (nucleoside triphosphate-diphosphohydrolases) play a key role in growth control in Arabidopsis."
      Wu J., Steinebrunner I., Sun Y., Butterfield T., Torres J., Arnold D., Gonzalez A., Jacob F., Reichler S., Roux S.J.
      Plant Physiol. 144:961-975(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: cv. Wassilewskija.
    9. "Hypertonic stress increased extracellular ATP levels and the expression of stress-responsive genes in Arabidopsis thaliana seedlings."
      Kim S.H., Yang S.H., Kim T.J., Han J.S., Suh J.W.
      Biosci. Biotechnol. Biochem. 73:1252-1256(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    10. "Extracellular nucleotides and apyrases regulate stomatal aperture in Arabidopsis."
      Clark G., Fraley D., Steinebrunner I., Cervantes A., Onyirimba J., Liu A., Torres J., Tang W., Kim J., Roux S.J.
      Plant Physiol. 156:1740-1753(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: cv. Wassilewskija.

    Entry informationi

    Entry nameiAPY1_ARATH
    AccessioniPrimary (citable) accession number: Q9SQG2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2012
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3