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Q9SQG2 (APY1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apyrase 1

Short name=AtAPY1
EC=3.6.1.5
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name=ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 1
Gene names
Name:APY1
Ordered Locus Names:At3g04080
ORF Names:T6K12.30
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Substrate preference is ATP > ADP. Functions with APY2 to reduce extracellular ATP level which is essential for pollen germination and normal plant development. Plays a role in the regulation of stomatal function by modulating extracellular ATP levels in guard cells. Ref.1 Ref.6 Ref.7 Ref.8 Ref.10

Catalytic activity

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactor

Calcium. Ref.1

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein. Membrane Probable; Single-pass type II membrane protein Probable. Note: As cell membrane protein, the functional domain could be at the extracellular side. Ref.2

Tissue specificity

Expressed in roots, root hairs, root cap, leaves, stems, trichomes, phloem throughout the plant, guard cells, filaments of young stamens, stipules, papillae of stigmas, pollen, pollen tubes and the abscission zone of siliques. Ref.1 Ref.6 Ref.7 Ref.8

Induction

By hypertonic stress. Ref.9

Disruption phenotype

No visible phenotype under normal growth conditions. Apy1 and apy2 double mutant displays developmental defects including the lack of functional root and shoot meristems, and morphogenetic and patterning abnormalities of the cotyledons. Double mutant exhibits a complete inhibition of pollen germination. Ref.6 Ref.7 Ref.8

Sequence similarities

Belongs to the GDA1/CD39 NTPase family.

Biophysicochemical properties

Kinetic parameters:

KM=30 µM for ATP Ref.1

Vmax=26 µmol/min/mg enzyme

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 471471Apyrase 1
PRO_0000419905

Regions

Topological domain1 – 2121Cytoplasmic Potential
Transmembrane22 – 4221Helical; Signal-anchor for type II membrane protein; Potential
Topological domain43 – 471429Lumenal Potential
Nucleotide binding72 – 8211ATP-binding Probable
Nucleotide binding218 – 22811ATP-binding Probable
Compositional bias350 – 3556Poly-Gly

Sites

Active site1941Proton acceptor By similarity

Amino acid modifications

Glycosylation3331N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9SQG2 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 6EF17A73207ECDA2

FASTA47151,194
        10         20         30         40         50         60 
MTAKRAIGRH ESLADKVHRH RGLLLVISIP IVLIALVLLL MPGTSTSVSV IEYTMKNHEG 

        70         80         90        100        110        120 
GSNSRGPKNY AVIFDAGSSG SRVHVYCFDQ NLDLVPLENE LELFLQLKPG LSAYPNDPRQ 

       130        140        150        160        170        180 
SANSLVTLLD KAEASVPREL RPKTPVRVGA TAGLRALGHQ ASENILQAVR ELLKGRSRLK 

       190        200        210        220        230        240 
TEANAVTVLD GTQEGSYQWV TINYLLRTLG KPYSDTVGVV DLGGGSVQMA YAIPEEDAAT 

       250        260        270        280        290        300 
APKPVEGEDS YVREMYLKGR KYFLYVHSYL HYGLLAARAE ILKVSEDSNN PCIATGYAGT 

       310        320        330        340        350        360 
YKYGGKAFKA AASPSGASLD ECRRVAINAL KVNNSLCTHM KCTFGGVWNG GGGGGQKKMF 

       370        380        390        400        410        420 
VASFFFDRAA EAGFVDPNQP VAEVRPLDFE KAANKACNMR MEEGKSKFPR VEEDNLPYLC 

       430        440        450        460        470 
LDLVYQYTLL VDGFGLKPSQ TITLVKKVKY GDYAVEAAWP LGSAIEAVSS P 

« Hide

References

« Hide 'large scale' references
[1]"Molecular and biochemical comparison of two different apyrases from Arabidopsis thaliana."
Steinebrunner I.A., Jeter C.R., Song C., Roux S.J.
Plant Physiol. Biochem. 38:913-922(2000)
Cited for: NUCLEOTIDE SEQUENCE [MRNA], COFACTOR, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[2]"Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis."
Parsons H.T., Christiansen K., Knierim B., Carroll A., Ito J., Batth T.S., Smith-Moritz A.M., Morrison S., McInerney P., Hadi M.Z., Auer M., Mukhopadhyay A., Petzold C.J., Scheller H.V., Loque D., Heazlewood J.L.
Plant Physiol. 159:12-26(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
Strain: cv. Landsberg erecta.
[3]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Arabidopsis ORF Clone."
Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Disruption of apyrases inhibits pollen germination in Arabidopsis."
Steinebrunner I., Wu J., Sun Y., Corbett A., Roux S.J.
Plant Physiol. 131:1638-1647(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
Strain: cv. Wassilewskija.
[7]"Developmental defects and seedling lethality in apyrase AtAPY1 and AtAPY2 double knockout mutants."
Wolf C., Hennig M., Romanovicz D., Steinebrunner I.
Plant Mol. Biol. 64:657-672(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
Strain: cv. Wassilewskija.
[8]"Apyrases (nucleoside triphosphate-diphosphohydrolases) play a key role in growth control in Arabidopsis."
Wu J., Steinebrunner I., Sun Y., Butterfield T., Torres J., Arnold D., Gonzalez A., Jacob F., Reichler S., Roux S.J.
Plant Physiol. 144:961-975(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
Strain: cv. Wassilewskija.
[9]"Hypertonic stress increased extracellular ATP levels and the expression of stress-responsive genes in Arabidopsis thaliana seedlings."
Kim S.H., Yang S.H., Kim T.J., Han J.S., Suh J.W.
Biosci. Biotechnol. Biochem. 73:1252-1256(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[10]"Extracellular nucleotides and apyrases regulate stomatal aperture in Arabidopsis."
Clark G., Fraley D., Steinebrunner I., Cervantes A., Onyirimba J., Liu A., Torres J., Tang W., Kim J., Roux S.J.
Plant Physiol. 156:1740-1753(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: cv. Wassilewskija.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF093604 mRNA. Translation: AAF00071.1.
JQ937231 mRNA. Translation: AFI41199.1.
AC016829 Genomic DNA. Translation: AAF26805.1.
CP002686 Genomic DNA. Translation: AEE74035.1.
BT029157 mRNA. Translation: ABJ17092.1.
RefSeqNP_187058.1. NM_111279.4.
UniGeneAt.16940.

3D structure databases

ProteinModelPortalQ9SQG2.
SMRQ9SQG2. Positions 70-462.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G04080.1-P.

Proteomic databases

PaxDbQ9SQG2.
PRIDEQ9SQG2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G04080.1; AT3G04080.1; AT3G04080.
GeneID819563.
KEGGath:AT3G04080.

Organism-specific databases

TAIRAT3G04080.

Phylogenomic databases

eggNOGCOG5371.
HOGENOMHOG000220904.
InParanoidQ9SQG2.
KOK14641.
OMAFQWVTIN.
PhylomeDBQ9SQG2.
ProtClustDBCLSN2684264.

Enzyme and pathway databases

BioCycARA:AT3G04080-MONOMER.

Gene expression databases

GenevestigatorQ9SQG2.

Family and domain databases

InterProIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERPTHR11782. PTHR11782. 1 hit.
PfamPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9SQG2.

Entry information

Entry nameAPY1_ARATH
AccessionPrimary (citable) accession number: Q9SQG2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names