Reviewed, UniProtKB/Swiss-Prot Q9SQ80 (G2OX1_PEA)

Names and origin

Protein names

Recommended name:
    Gibberellin 2-beta-dioxygenase 1
    EC=1.14.11.13
Alternative name(s):
    Gibberellin 2-beta-hydroxylase 1
    Gibberellin 2-oxidase 1
    GA 2-oxidase 1
    Protein SLENDER

Gene names

Name:	GA2OX1
Synonyms:	SLN

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins, leading to the homeostatic regulation of their endogenous level. Catabolism of gibberellins (GAs) plays a central role in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to GA34/GA8. Ref.1 Ref.2
Catalytic activity	Gibberellin 1 + 2-oxoglutarate + O2 = 2-beta-hydroxygibberellin 1 + succinate + CO2.
Cofactor	Iron By similarity.
Pathway	Plant hormone biosynthesis; gibberellin biosynthesis.
Tissue specificity	Predominantly expressed in roots, flowers, young fruits and seeds. Ref.1 Ref.2
Disruption phenotype	Plants display to the slender phenotype, characterized by large amounts of GA20 that cannot be degraded, and is metabolized to excess GA1 on germination, producing plants with a characteristic slender or hyperelongated phenotype. Ref.1
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily. Contains 1 Fe2OG dioxygenase domain.
Biophysicochemical properties	Kinetic parameters: KM=0.024 µM for GA20 Vmax=4.4 pmol/sec/mg enzyme

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	gibberellin 2-beta-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Gibberellin 2-beta-dioxygenase 1		PRO_0000067311
Regions
	Domain	171 – 276	106	Fe2OG dioxygenase
Sites
	Active site	267	1	Potential
	Metal binding	200	1	Iron By similarity
	Metal binding	202	1	Iron By similarity
	Metal binding	257	1	Iron By similarity
Experimental info
	Sequence conflict	116	1	D → E in AAF13734. Ref.1
	Sequence conflict	116	1	D → E in AAD45425. Ref.2
	Sequence conflict	127	1	D → H in AAF08609. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9SQ80-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2728A3B5C871CFB2	FASTA	327	36,811
	10 20 30 40 50 60 MVLLSKPTSE QYTYVRNNMP ITFSSSIPLV DLSKPDAKTL IVKACEDFGF FKVINHGIPL 70 80 90 100 110 120 DAISQLESEA FKFFSLPQTE KEKAGPANPF GYGNKRIGLN GDIGWIEYLL LTTNQDHNFS 130 140 150 160 170 180 LYGEDIDKFR GLLKDYKCAM RNMACEILDL MAEGLKIQPK NVFSKLVMDK QSDCLFRVNH 190 200 210 220 230 240 YPACPELAIN GENLIGFGEH TDPQIISILR SNNTSGFQIS LRDGSWISVP PDHSSFFINV 250 260 270 280 290 300 GDSLQVMTNG RFKSVRHRVL ANGIDPRLSM IYFCGPPLSE KIAPLPSLMK GKESLYKEFT 310 320 WFEYKSSTYG SRLADNRLGN YERIAAT

References

[1]	"The SLENDER gene of pea encodes a gibberellin 2-oxidase." Martin D.N., Proebsting W.M., Hedden P. Plant Physiol. 121:775-781(1999) [PubMed: 10557225] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. Strain: cv. Alaska. Tissue: Seed.
[2]	"Gibberellin 2-oxidation and the SLN gene of Pisum sativum." Lester D.R., Ross J.J., Smith J.J., Elliott R.C., Reid J.B. Plant J. 19:65-73(1999) [PubMed: 10417727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-327, FUNCTION, TISSUE SPECIFICITY. Strain: cv. Torsdag. Tissue: Seed.
[3]	"Purification and kinetic studies of recombinant gibberellin dioxygenases." Lester D.R., Phillips A., Hedden P., Andersson I. BMC Plant Biol. 5:19-19(2005) [PubMed: 16181493] [Abstract] Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF056935 mRNA. Translation: AAF08609.1. AF101382 mRNA. Translation: AAF13734.1. Sequence problems. AF101383 Genomic DNA. Translation: AAF13735.1. AF100955 mRNA. Translation: AAD45425.1.
3D structure databases
SMR	Q9SQ80. Positions 26-313.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11639.
BRENDA	1.14.11.13. 287.
Family and domain databases
InterPro	IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G2OX1_PEA

Accession

Primary (citable) accession number: Q9SQ80
Secondary accession number(s): Q9SQ81, Q9SQJ0, Q9XHM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)