Reviewed, UniProtKB/Swiss-Prot Q9SPV5 (NEC1_NICPL)

Names and origin

Protein names

Recommended name:
    Nectarin-1
    EC=1.15.1.1
Alternative name(s):
    Superoxide dismutase [Mn]

Gene names

Name:

NEC1

Organism

Nicotiana plumbaginifolia (Leadwort-leaved tobacco)

Taxonomic identifier

4092 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May interact with bacterial adhesins thereby protecting the reproductive tissues from microbial attack. Has no oxalate oxidase activity By similarity.
Catalytic activity	2 superoxide + 2 H+ = O2 + H2O2.
Cofactor	Binds 1 manganese ion per monomer By similarity.
Subunit structure	Monomer. In the absence of manganese, it forms tetrameric and pentameric forms which show superoxide dismutase activity By similarity.
Subcellular location	Secreted › extracellular space › apoplast By similarity. Note: Secreted in the nectar By similarity.
Post-translational modification	Glycosylated By similarity.
Sequence similarities	Belongs to the germin family.
Biophysicochemical properties	Temperature dependence: Highly thermostable.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Ligand	Manganese Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nutrient reservoir activity Inferred from electronic annotation. Source: InterPro superoxide dismutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	By similarity
	Chain	33 – 229	197	Nectarin-1		PRO_0000010841
Sites
	Metal binding	117	1	Manganese By similarity
	Metal binding	119	1	Manganese By similarity
	Metal binding	124	1	Manganese By similarity
	Metal binding	163	1	Manganese By similarity
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 57		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SPV5-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: DBB8AE087F8256DE	FASTA	229	24,765
	10 20 30 40 50 60 MAAFGIKSKI FQIMEMTILF LFAISIDRYC FAADEDMLQD VCVADLHSKV KVNGFPCKTN 70 80 90 100 110 120 FTAADFSSFA ISKPGATNNK FGSKVTTANV EQVPGLNTLG VSLARIDYAP GGINPPHTHP 130 140 150 160 170 180 RASEMVFVME GELDVGFITT ANVLVSKQIT KGEVFVFPRG LVHFQKNNGK IPAAVVSAFN 190 200 210 220 SQLPGTQSIP ITLFGASPTV PDDVLAQTFQ INIEDVQQIK SKFAPAKKF

References

[1]	"Nectarin I is a novel, soluble germin-like protein expressed in the nectar of Nicotiana sp." Carter C., Graham R.A., Thornburg R.W. Plant Mol. Biol. 41:207-216(1999) [PubMed: 10579488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 129-137.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF132671 Genomic DNA. Translation: AAF03355.1.
3D structure databases
SMR	Q9SPV5. Positions 33-225.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.15.1.1. 257040.
Family and domain databases
InterPro	IPR006045. Cupin_1. IPR011051. Cupin_RmlC_type. IPR001929. Germin. IPR019780. Germin_Mn-BS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF00190. Cupin_1. 1 hit. [Graphical view]
PRINTS	PR00325. GERMIN.
SMART	SM00835. Cupin_1. 1 hit. [Graphical view]
PROSITE	PS00725. GERMIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEC1_NICPL

Accession

Primary (citable) accession number: Q9SPV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)