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Protein

Telomerase reverse transcriptase

Gene

TERT

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Required to prevent genome instability induced by breakage-fusion-bridge (BFB) cycles. Can extend completely non-telomeric sequences using RNA template in vitro.4 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi691Magnesium; catalyticPROSITE-ProRule annotation1
Metal bindingi860Magnesium; catalyticPROSITE-ProRule annotation1
Metal bindingi861Magnesium; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • chromosome localization Source: TAIR
  • chromosome organization Source: TAIR
  • meristem maintenance Source: TAIR
  • nucleolus organization Source: TAIR
  • root meristem growth Source: TAIR
  • stem cell population maintenance Source: TAIR
  • telomere maintenance Source: TAIR
  • telomere maintenance via telomerase Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed DNA polymerase, Transferase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-171319. Telomere Extension By Telomerase.

Names & Taxonomyi

Protein namesi
Recommended name:
Telomerase reverse transcriptase (EC:2.7.7.49)
Short name:
AtTERT
Gene namesi
Name:TERT
Ordered Locus Names:At5g16850
ORF Names:F5E19.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G16850.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: UniProtKB-SubCell
  • nucleus Source: TAIR
  • telomerase catalytic core complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

Pathology & Biotechi

Disruption phenotypei

Low rates of telomere shortening at each generation. Null mutant plants can survive up to 10 generations before dying. Accelerated proliferation and cell death in dedifferentiated cells.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004126071 – 1123Telomerase reverse transcriptaseAdd BLAST1123

Proteomic databases

PaxDbiQ9SPU7.

Expressioni

Gene expression databases

GenevisibleiQ9SPU7. AT.

Interactioni

Subunit structurei

Component of the telomerase ribonucleoprotein complex (Probable). Interacts with POT1A.Curated1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
POT1AQ56Y524EBI-1606133,EBI-1606062

Protein-protein interaction databases

BioGridi16824. 3 interactors.
IntActiQ9SPU7. 1 interactor.
STRINGi3702.AT5G16850.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SPU7.
SMRiQ9SPU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini596 – 929Reverse transcriptasePROSITE-ProRule annotationAdd BLAST334

Sequence similaritiesi

Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1005. Eukaryota.
ENOG410XQJH. LUCA.
HOGENOMiHOG000153522.
InParanoidiQ9SPU7.
KOiK11126.
OMAiSSKLCHY.
OrthoDBiEOG09360247.
PhylomeDBiQ9SPU7.

Family and domain databases

InterProiIPR000477. RT_dom.
IPR021891. Telomerase_RBD.
IPR003545. Telomerase_RT.
[Graphical view]
PANTHERiPTHR12066:SF0. PTHR12066:SF0. 1 hit.
PfamiPF12009. Telomerase_RBD. 1 hit.
[Graphical view]
PRINTSiPR01365. TELOMERASERT.
SMARTiSM00975. Telomerase_RBD. 1 hit.
[Graphical view]
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SPU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRKPRHRVP EILWRLFGNR ARNLNDAIVD LIPNRNIQPE QCRCRGQGCL
60 70 80 90 100
GCSSDKPAFL LRSDDPIHYR KLLHRCFVVL HEQTPPLLDF SPTSWWSQRE
110 120 130 140 150
IVERIIEMMQ SGCDCQNVIC ARYDKYDQSS PILELLTSSS WEFLLKRVGH
160 170 180 190 200
DVMVYLLQQT SIFLPLLGKK HQQVSGPPLC IKHKRTLSVH ENKRKRDDNV
210 220 230 240 250
QPPTKRQWLS SAVDDCPKDD SATITPIVGE DVDQHREKKT TKRSRIYLKR
260 270 280 290 300
RRKQRKVNFK KVDCNAPCIT PSTNGKVSTG NDEMNLHIGI NGSLTDFVKQ
310 320 330 340 350
AKQVKRNKNF KFGLSETYSV IPPNHILKTL RPNCSDSKLL MNHIFGEVNV
360 370 380 390 400
WSTTPSHGKG NCPSGSICLY HSLLKSLKNL IGKTKSSHLK MLLDKHCPVL
410 420 430 440 450
LLQEDALKSG TTSQSSRRQK ADKLPHGSSS SQTGKPKCPS VEERKLYCTN
460 470 480 490 500
DQVVSFIWAI CRYIVPESLL GTTHQMRVLR KNIAWFVSRR RNEKCTVNQF
510 520 530 540 550
LHKVKPSDFP FFARKELCCM VNGHELQSES IRSTQQMLCT KWISWLFLEI
560 570 580 590 600
VKKLVHFNFY ATESQGGRLN IYYYRKRSWE RLISKEISKA LDGYVLVDDA
610 620 630 640 650
EAESSRKKLS KFRFLPKANG VRMVLDFSSS SRSQSLRDTH AVLKDIQLKE
660 670 680 690 700
PDVLGSSVFD HDDFYRNLCP YLIHLRSQSG ELPPLYFVVA DVFKAFDSVD
710 720 730 740 750
QGKLLHVIQS FLKDEYILNR CRLVCCGKRS NWVNKILVSS DKNSNFSRFT
760 770 780 790 800
STVPYNALQS IVVDKGENHR VRKKDLMVWI GNMLKNNMLQ LDKSFYVQIA
810 820 830 840 850
GIPQGHRLSS LLCCFYYGHL ERTLIYPFLE EASKDVSSKE CSREEELIIP
860 870 880 890 900
TSYKLLRFID DYLFVSTSRD QASSFYHRLK HGFKDYNCFM NETKFCINFE
910 920 930 940 950
DKEEHRCSSN RMFVGDNGVP FVRWTGLLIN SRTFEVQVDY TRYLSGHISS
960 970 980 990 1000
TFSVAWQNKP VRNLRQKLCY FLVPKCHPIL FDSNINSGEI VRLNIYQIFL
1010 1020 1030 1040 1050
LAAMKFHCYV YEVSRFWKLH PQTLFKFITI SVRYMFRLIN RRVRRINTGS
1060 1070 1080 1090 1100
SFRPVLKLYK EEVIWLGLDA YIQVLKKKNS RYRMLLIYLK SALSKHSLSQ
1110 1120
QLSSELRYAT DRSNSSSLWK LNY
Length:1,123
Mass (Da):130,580
Last modified:May 1, 2000 - v1
Checksum:i5645B8295817B7F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1107R → K in AAD54276 (PubMed:10611295).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135454 mRNA. Translation: AAD54777.1.
AF172097 Genomic DNA. Translation: AAD54276.1.
AL391147 Genomic DNA. Translation: CAC01849.1.
CP002688 Genomic DNA. Translation: AED92349.1.
PIRiT51517.
RefSeqiNP_197187.1. NM_121691.4.
UniGeneiAt.49030.
At.70246.

Genome annotation databases

EnsemblPlantsiAT5G16850.1; AT5G16850.1; AT5G16850.
GeneIDi831548.
GrameneiAT5G16850.1; AT5G16850.1; AT5G16850.
KEGGiath:AT5G16850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135454 mRNA. Translation: AAD54777.1.
AF172097 Genomic DNA. Translation: AAD54276.1.
AL391147 Genomic DNA. Translation: CAC01849.1.
CP002688 Genomic DNA. Translation: AED92349.1.
PIRiT51517.
RefSeqiNP_197187.1. NM_121691.4.
UniGeneiAt.49030.
At.70246.

3D structure databases

ProteinModelPortaliQ9SPU7.
SMRiQ9SPU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16824. 3 interactors.
IntActiQ9SPU7. 1 interactor.
STRINGi3702.AT5G16850.1.

Proteomic databases

PaxDbiQ9SPU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G16850.1; AT5G16850.1; AT5G16850.
GeneIDi831548.
GrameneiAT5G16850.1; AT5G16850.1; AT5G16850.
KEGGiath:AT5G16850.

Organism-specific databases

TAIRiAT5G16850.

Phylogenomic databases

eggNOGiKOG1005. Eukaryota.
ENOG410XQJH. LUCA.
HOGENOMiHOG000153522.
InParanoidiQ9SPU7.
KOiK11126.
OMAiSSKLCHY.
OrthoDBiEOG09360247.
PhylomeDBiQ9SPU7.

Enzyme and pathway databases

ReactomeiR-ATH-171319. Telomere Extension By Telomerase.

Miscellaneous databases

PROiQ9SPU7.

Gene expression databases

GenevisibleiQ9SPU7. AT.

Family and domain databases

InterProiIPR000477. RT_dom.
IPR021891. Telomerase_RBD.
IPR003545. Telomerase_RT.
[Graphical view]
PANTHERiPTHR12066:SF0. PTHR12066:SF0. 1 hit.
PfamiPF12009. Telomerase_RBD. 1 hit.
[Graphical view]
PRINTSiPR01365. TELOMERASERT.
SMARTiSM00975. Telomerase_RBD. 1 hit.
[Graphical view]
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTERT_ARATH
AccessioniPrimary (citable) accession number: Q9SPU7
Secondary accession number(s): Q9SE99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In the absence of telomerase, telomeres decline by approximately 500 bp per generation, a rate 10 times slower than seen in telomerase-deficient mice. This may be due to alternative telomere lengthening, a process which is activated in early embryonic development.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.