Q9SPP9 (Q9SPP9_RAUSE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Raucaffricine-O-beta-D-glucosidase EMBL AAF03675.1 EC=3.2.1.125 EMBL AAF03675.1
Organism	Rauvolfia serpentina (Serpentwood) (Devilpepper) EMBL AAF03675.1
Taxonomic identifier	4060 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Rauvolfia

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the glycosyl hydrolase 1 family. RuleBase RU003690

Ontologies

Keywords
Molecular function	Glycosidase EMBL AAF03675.1 Hydrolase
Technical term	3D-structure PDB 3U57 PDB 3U5U PDB 3U5Y PDB 4A3Y PDB 4ATD PDB 4ATL PDB 4EK7
Gene Ontology (GO)
Biological_process	alkaloid biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	raucaffricine beta-glucosidase activity Inferred from direct assay Ref.1. Source: UniProtKB vomilenine glucosyltransferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q9SPP9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 543A24654A4D5E7D	FASTA	540	60,934
	10 20 30 40 50 60 MATQSSAVID SNDATRISRS DFPADFIMGT GSSAYQIEGG ARDGGRGPSI WDTFTHRRPD 70 80 90 100 110 120 MIRGGTNGDV AVDSYHLYKE DVNILKNLGL DAYRFSISWS RVLPGGRLSG GVNKEGINYY 130 140 150 160 170 180 NNLIDGLLAN GIKPFVTLFH WDVPQALEDE YGGFLSPRIV DDFCEYAELC FWEFGDRVKH 190 200 210 220 230 240 WMTLNEPWTF SVHGYATGLY APGRGRTSPE HVNHPTVQHR CSTVAPQCIC STGNPGTEPY 250 260 270 280 290 300 WVTHHLLLAH AAAVELYKNK FQRGQEGQIG ISHATQWMEP WDENSASDVE AAARALDFML 310 320 330 340 350 360 GWFMEPITSG DYPKSMKKFV GSRLPKFSPE QSKMLKGSYD FVGLNYYTAS YVTNASTNSS 370 380 390 400 410 420 GSNNFSYNTD IHVTYETDRN GVPIGPQSGS DWLLIYPEGI RKILVYTKKT YNVPLIYVTE 430 440 450 460 470 480 NGVDDVKNTN LTLSEARKDS MRLKYLQDHI FNVRQAMNDG VNVKGYFAWS LLDNFEWGEG 490 500 510 520 530 540 YGVRFGIIHI DYNDNFARYP KDSAVWLMNS FHKNISKLPA VKRSIREDDE EQVSSKRLRK

References

[1]	"Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis." Warzecha H., Gerasimenko I., Kutchan T.M., Stockigt J. Phytochemistry 54:657-666(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: BENTH. ex KURZ EMBL AAF03675.1.
[2]	"Structures of alkaloid biosynthetic glucosidases decode substrate specificity." Xia L., Ruppert M., Wang M., Panjikar S., Lin H., Rajendran C., Barleben L., Stockigt J. ACS Chem. Biol. 7:226-234(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
[3]	"High speed X-ray analysis of plant enzymes at room temperature." Xia L., Rajendran C., Ruppert M., Panjikar S., Wang M., Stoeckigt J. Phytochemistry 0:0-0(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 1-513.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF149311 mRNA. Translation: AAF03675.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3U57	X-ray	2.43	A/B	1-513	[»]
3U5U	X-ray	2.20	A/B	1-513	[»]
3U5Y	X-ray	2.30	A/B	1-513	[»]
4A3Y	X-ray	2.15	A/B	1-540	[»]
4ATD	X-ray	2.10	A/B	1-513	[»]
4ATL	X-ray	2.52	A/B	1-513	[»]
4EK7	X-ray	2.30	A/B	1-513	[»]

ProteinModelPortal

Q9SPP9.

SMR

Q9SPP9. Positions 17-508.

ModBase

Search...

Protein family/group databases

CAZy

GH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

3.2.1.125. 257570.

Family and domain databases

Gene3D

3.20.20.80. 1 hit.

InterPro

IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

PANTHER

PTHR10353. PTHR10353. 1 hit.

Pfam

PF00232. Glyco_hydro_1. 1 hit.
[Graphical view]

PRINTS

PR00131. GLHYDRLASE1.

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q9SPP9_RAUSE

Accession

Primary (citable) accession number: Q9SPP9

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 2000
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)