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Protein

Raucaffricine-O-beta-D-glucosidase

Gene

RG

Organism
Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glucosidase specifically involved in alkaloid biosynthesis leading to the accumulation of several alkaloids, including ajmaline, an important plant-derived pharmaceutical used in the treatment of heart disorders.1 Publication

Catalytic activityi

Raucaffricine + H2O = D-glucose + vomilenine.1 Publication
UDP-glucose + vomilenine = UDP + raucaffricine.1 Publication

Kineticsi

  1. KM=1.3 mM for raucaffricine (at pH 5 and 28 degrees Celsius, PubMed:22004291, PubMed:10975500)2 Publications
  2. KM=1.8 mM for strictosidine (at pH 5 and 28 degrees Celsius, PubMed:22004291, PubMed:10975500)2 Publications
  1. Vmax=0.5 nmol/sec/µg enzyme with raucaffricine as substrate (at pH 5 and 28 degrees Celsius, PubMed:22004291, PubMed:10975500)2 Publications
  2. Vmax=2.6 pmol/sec/µg enzyme with strictosidine as substrate (at pH 5 and 28 degrees Celsius, PubMed:22004291, PubMed:10975500)2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36Substrate1 Publication1
Binding sitei140SubstrateBy similarity1
Active sitei186Proton donor1
Binding sitei193Substrate1 Publication1
Binding sitei347SubstrateBy similarity1
Sitei390Directs the conformation of W-3921
Binding sitei392Substrate1 Publication1
Sitei392Controls the gate shape and acceptance of substrates1
Active sitei420Nucleophile1
Binding sitei420Substrate1 Publication1
Binding sitei469Substrate1 Publication1

GO - Molecular functioni

  • raucaffricine beta-glucosidase activity Source: UniProtKB
  • vomilenine glucosyltransferase activity Source: UniProtKB

GO - Biological processi

  • alkaloid biosynthetic process Source: UniProtKB
  • carbohydrate metabolic process Source: InterPro
  • metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Alkaloid metabolism

Enzyme and pathway databases

BRENDAi3.2.1.125. 5309.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Raucaffricine-O-beta-D-glucosidase (EC:3.2.1.125)
Short name:
Raucaffricine beta-glucosidase
Short name:
RsRG
Alternative name(s):
Vomilenine glucosyltransferase (EC:2.4.1.219)
Short name:
RsVGT
Gene namesi
Name:RG
Synonyms:VGT
OrganismiRauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum)
Taxonomic identifieri4060 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeRauvolfiinaeRauvolfia

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi186E → D or Q: Loss of activity. 1 Publication1
Mutagenesisi189T → A: Reduced activity. 1 Publication1
Mutagenesisi193H → A: Reduced activity. 1 Publication1
Mutagenesisi200Y → A: Loss of activity. 1 Publication1
Mutagenesisi390S → G: Reduced activity. 1 Publication1
Mutagenesisi392W → A: Loss of activity. 1 Publication1
Mutagenesisi420E → Q: Loss of activity. 1 Publication1
Mutagenesisi476E → A or L: Loss of activity. 1 Publication1
Mutagenesisi485F → Y: Reduced activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004184001 – 540Raucaffricine-O-beta-D-glucosidaseAdd BLAST540

Structurei

Secondary structure

1540
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 16Combined sources3
Helixi19 – 21Combined sources3
Beta strandi27 – 31Combined sources5
Helixi34 – 37Combined sources4
Helixi50 – 57Combined sources8
Helixi59 – 61Combined sources3
Helixi63 – 65Combined sources3
Turni68 – 72Combined sources5
Helixi74 – 88Combined sources15
Beta strandi91 – 96Combined sources6
Helixi99 – 102Combined sources4
Helixi108 – 110Combined sources3
Helixi114 – 129Combined sources16
Beta strandi133 – 141Combined sources9
Helixi145 – 151Combined sources7
Helixi153 – 155Combined sources3
Helixi159 – 174Combined sources16
Turni175 – 177Combined sources3
Beta strandi180 – 185Combined sources6
Helixi187 – 195Combined sources9
Turni235 – 237Combined sources3
Helixi238 – 260Combined sources23
Helixi262 – 265Combined sources4
Beta strandi268 – 282Combined sources15
Helixi286 – 299Combined sources14
Helixi301 – 304Combined sources4
Helixi306 – 309Combined sources4
Helixi314 – 320Combined sources7
Helixi321 – 323Combined sources3
Helixi329 – 335Combined sources7
Beta strandi340 – 354Combined sources15
Helixi367 – 370Combined sources4
Beta strandi373 – 376Combined sources4
Beta strandi378 – 381Combined sources4
Beta strandi382 – 385Combined sources4
Helixi398 – 411Combined sources14
Beta strandi414 – 421Combined sources8
Helixi433 – 436Combined sources4
Helixi440 – 458Combined sources19
Beta strandi463 – 469Combined sources7
Helixi477 – 479Combined sources3
Beta strandi482 – 484Combined sources3
Beta strandi487 – 490Combined sources4
Turni492 – 496Combined sources5
Beta strandi498 – 500Combined sources3
Helixi502 – 511Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3U57X-ray2.43A/B1-513[»]
3U5UX-ray2.20A/B1-513[»]
3U5YX-ray2.30A/B1-513[»]
3ZJ6X-ray2.40A/B1-540[»]
4A3YX-ray2.15A/B1-540[»]
4ATDX-ray2.10A/B1-513[»]
4ATLX-ray2.52A/B1-513[»]
4EK7X-ray2.30A/B1-513[»]
ProteinModelPortaliQ9SPP9.
SMRiQ9SPP9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 186Substrate binding2
Regioni476 – 477Substrate binding2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Phylogenomic databases

KOiK12695.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 2 hits.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SPP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATQSSAVID SNDATRISRS DFPADFIMGT GSSAYQIEGG ARDGGRGPSI
60 70 80 90 100
WDTFTHRRPD MIRGGTNGDV AVDSYHLYKE DVNILKNLGL DAYRFSISWS
110 120 130 140 150
RVLPGGRLSG GVNKEGINYY NNLIDGLLAN GIKPFVTLFH WDVPQALEDE
160 170 180 190 200
YGGFLSPRIV DDFCEYAELC FWEFGDRVKH WMTLNEPWTF SVHGYATGLY
210 220 230 240 250
APGRGRTSPE HVNHPTVQHR CSTVAPQCIC STGNPGTEPY WVTHHLLLAH
260 270 280 290 300
AAAVELYKNK FQRGQEGQIG ISHATQWMEP WDENSASDVE AAARALDFML
310 320 330 340 350
GWFMEPITSG DYPKSMKKFV GSRLPKFSPE QSKMLKGSYD FVGLNYYTAS
360 370 380 390 400
YVTNASTNSS GSNNFSYNTD IHVTYETDRN GVPIGPQSGS DWLLIYPEGI
410 420 430 440 450
RKILVYTKKT YNVPLIYVTE NGVDDVKNTN LTLSEARKDS MRLKYLQDHI
460 470 480 490 500
FNVRQAMNDG VNVKGYFAWS LLDNFEWGEG YGVRFGIIHI DYNDNFARYP
510 520 530 540
KDSAVWLMNS FHKNISKLPA VKRSIREDDE EQVSSKRLRK
Length:540
Mass (Da):60,934
Last modified:May 1, 2000 - v1
Checksum:i543A24654A4D5E7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149311 mRNA. Translation: AAF03675.1.

Genome annotation databases

KEGGiag:AAF03675.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149311 mRNA. Translation: AAF03675.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3U57X-ray2.43A/B1-513[»]
3U5UX-ray2.20A/B1-513[»]
3U5YX-ray2.30A/B1-513[»]
3ZJ6X-ray2.40A/B1-540[»]
4A3YX-ray2.15A/B1-540[»]
4ATDX-ray2.10A/B1-513[»]
4ATLX-ray2.52A/B1-513[»]
4EK7X-ray2.30A/B1-513[»]
ProteinModelPortaliQ9SPP9.
SMRiQ9SPP9.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAF03675.

Phylogenomic databases

KOiK12695.

Enzyme and pathway databases

BRENDAi3.2.1.125. 5309.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 2 hits.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRG1_RAUSE
AccessioniPrimary (citable) accession number: Q9SPP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 11, 2013
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.