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Protein

Apyrase 2

Gene

APY2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Substrate preference is ATP > ADP. Functions with APY1 to reduce extracellular ATP level which is essential for pollen germination and normal plant development. Plays a role in the regulation of stomatal function by modulating extracellular ATP levels in guard cells.3 Publications

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Ca2+By similarity

Kineticsi

  1. KM=30 µM for ATP1 Publication
  1. Vmax=26 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei195Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi73 – 83ATP-bindingCuratedAdd BLAST11
Nucleotide bindingi219 – 229ATP-bindingCuratedAdd BLAST11

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G18280-MONOMER.
ReactomeiR-ATH-8850843. Phosphate bond hydrolysis by NTPDase proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Apyrase 2 (EC:3.6.1.5)
Short name:
AtAPY2
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name:
ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 2
Gene namesi
Name:APY2
Ordered Locus Names:At5g18280
ORF Names:MRG7.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 42Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini43 – 472LumenalSequence analysisAdd BLAST430

GO - Cellular componenti

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions. Apy1 and apy2 double mutant displays developmental defects including the lack of functional root and shoot meristems, and morphogenetic and patterning abnormalities of the cotyledons. Double mutant exhibits a complete inhibition of pollen germination.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_000041990648 – 472Apyrase 2Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi334N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in roots, root hairs, root cap, leaves, stems, trichomes, phloem throughout the plant, guard cells, filaments of young stamens, stipules, papillae of stigmas, pollen, pollen tubes and the abscission zone of siliques.3 Publications

Inductioni

By hypertonic stress.

Gene expression databases

GenevisibleiQ9SPM5. AT.

Structurei

3D structure databases

ProteinModelPortaliQ9SPM5.
SMRiQ9SPM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000220904.
InParanoidiQ9SPM5.
KOiK14641.
PhylomeDBiQ9SPM5.

Family and domain databases

InterProiView protein in InterPro
IPR000407. GDA1_CD39_NTPase.
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiView protein in Pfam
PF01150. GDA1_CD39. 1 hit.
PROSITEiView protein in PROSITE
PS01238. GDA1_CD39_NTPASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SPM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAKRGIGRH ESLADKIQRH RGIILVISVP IVLIGLVLLL MPGRSISDSV
60 70 80 90 100
VEEYSVHNRK GGPNSRGPKN YAVIFDAGSS GSRVHVYCFD QNLDLIPLGN
110 120 130 140 150
ELELFLQLKP GLSAYPTDPR QAANSLVSLL DKAEASVPRE LRPKTHVRVG
160 170 180 190 200
ATAGLRTLGH DASENILQAV RELLRDRSML KTEANAVTVL DGTQEGSYQW
210 220 230 240 250
VTINYLLRNL GKPYSDTVGV VDLGGGSVQM AYAISEEDAA SAPKPLEGED
260 270 280 290 300
SYVREMYLKG RKYFLYVHSY LHYGLLAARA EILKVSEDSE NPCIVAGYDG
310 320 330 340 350
MYKYGGKEFK APASQSGASL DECRRITINA LKVNDTLCTH MKCTFGGVWN
360 370 380 390 400
GGRGGGQKNM FVASFFFDRA AEAGFVDPKQ PVATVRPMDF EKAAKKACSM
410 420 430 440 450
KLEEGKSTFP LVEEENLPYL CMDLVYQYTL LIDGFGLEPS QTITLVKKVK
460 470
YGDQAVEAAW PLGSAIEAVS SP
Length:472
Mass (Da):51,599
Last modified:May 1, 2000 - v1
Checksum:iFA0C280456D51224
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20H → N in AFI41206 (PubMed:22430844).Curated1
Sequence conflicti151A → V in AAF66599 (Ref. 2) Curated1
Sequence conflicti261R → Q in AAM98186 (PubMed:14593172).Curated1
Sequence conflicti301M → T in AAF66599 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156783 mRNA. Translation: AAF00612.1.
AF141671 mRNA. Translation: AAF66599.1.
JQ937238 mRNA. Translation: AFI41206.1.
AB012246 Genomic DNA. Translation: BAB09486.1.
CP002688 Genomic DNA. Translation: AED92532.2.
AY099866 mRNA. Translation: AAM20717.1.
AY140045 mRNA. Translation: AAM98186.1.
BT000329 mRNA. Translation: AAN15648.1.
RefSeqiNP_197329.5. NM_121833.7.
UniGeneiAt.19874.

Genome annotation databases

EnsemblPlantsiAT5G18280.1; AT5G18280.1; AT5G18280.
GeneIDi831946.
GrameneiAT5G18280.1; AT5G18280.1; AT5G18280.
KEGGiath:AT5G18280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156783 mRNA. Translation: AAF00612.1.
AF141671 mRNA. Translation: AAF66599.1.
JQ937238 mRNA. Translation: AFI41206.1.
AB012246 Genomic DNA. Translation: BAB09486.1.
CP002688 Genomic DNA. Translation: AED92532.2.
AY099866 mRNA. Translation: AAM20717.1.
AY140045 mRNA. Translation: AAM98186.1.
BT000329 mRNA. Translation: AAN15648.1.
RefSeqiNP_197329.5. NM_121833.7.
UniGeneiAt.19874.

3D structure databases

ProteinModelPortaliQ9SPM5.
SMRiQ9SPM5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G18280.1; AT5G18280.1; AT5G18280.
GeneIDi831946.
GrameneiAT5G18280.1; AT5G18280.1; AT5G18280.
KEGGiath:AT5G18280.

Organism-specific databases

AraportiAT5G18280.

Phylogenomic databases

HOGENOMiHOG000220904.
InParanoidiQ9SPM5.
KOiK14641.
PhylomeDBiQ9SPM5.

Enzyme and pathway databases

BioCyciARA:AT5G18280-MONOMER.
ReactomeiR-ATH-8850843. Phosphate bond hydrolysis by NTPDase proteins.

Miscellaneous databases

PROiPR:Q9SPM5.

Gene expression databases

GenevisibleiQ9SPM5. AT.

Family and domain databases

InterProiView protein in InterPro
IPR000407. GDA1_CD39_NTPase.
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiView protein in Pfam
PF01150. GDA1_CD39. 1 hit.
PROSITEiView protein in PROSITE
PS01238. GDA1_CD39_NTPASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPY2_ARATH
AccessioniPrimary (citable) accession number: Q9SPM5
Secondary accession number(s): F4JWK7
, I1VCB1, Q8L704, Q9M7B3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: May 1, 2000
Last modified: June 7, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.