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Q9SPM5

- APY2_ARATH

UniProt

Q9SPM5 - APY2_ARATH

Protein

Apyrase 2

Gene

APY2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Substrate preference is ATP > ADP. Functions with APY1 to reduce extracellular ATP level which is essential for pollen germination and normal plant development. Plays a role in the regulation of stomatal function by modulating extracellular ATP levels in guard cells.3 Publications

    Catalytic activityi

    A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

    Cofactori

    Calcium.By similarity

    Kineticsi

    1. KM=30 µM for ATP1 Publication

    Vmax=26 µmol/min/mg enzyme1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei195 – 1951Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi73 – 8311ATP-bindingCuratedAdd
    BLAST
    Nucleotide bindingi219 – 22911ATP-bindingCuratedAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrolase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Calcium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G18280-MONOMER.
    ARA:GQT-2442-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apyrase 2 (EC:3.6.1.5)
    Short name:
    AtAPY2
    Alternative name(s):
    ATP-diphosphatase
    ATP-diphosphohydrolase
    Adenosine diphosphatase
    Short name:
    ADPase
    NTPDase
    Nucleoside triphosphate diphosphohydrolase 2
    Gene namesi
    Name:APY2
    Ordered Locus Names:At5g18280
    ORF Names:MRG7.24
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G18280.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication. Membrane 1 Publication; Single-pass type II membrane protein 1 Publication
    Note: As cell membrane protein, the functional domain could be at the extracellular side.

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions. Apy1 and apy2 double mutant displays developmental defects including the lack of functional root and shoot meristems, and morphogenetic and patterning abnormalities of the cotyledons. Double mutant exhibits a complete inhibition of pollen germination.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini48 – 472425Apyrase 2PRO_0000419906Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ9SPM5.

    Expressioni

    Tissue specificityi

    Expressed in roots, root hairs, root cap, leaves, stems, trichomes, phloem throughout the plant, guard cells, filaments of young stamens, stipules, papillae of stigmas, pollen, pollen tubes and the abscission zone of siliques.3 Publications

    Inductioni

    By hypertonic stress.

    Gene expression databases

    GenevestigatoriQ9SPM5.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SPM5.
    SMRiQ9SPM5. Positions 71-463.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2121CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini43 – 472430LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei22 – 4221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GDA1/CD39 NTPase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    InParanoidiQ9SPM5.
    KOiK14641.
    PhylomeDBiQ9SPM5.

    Family and domain databases

    InterProiIPR000407. GDA1_CD39_NTPase.
    [Graphical view]
    PANTHERiPTHR11782. PTHR11782. 1 hit.
    PfamiPF01150. GDA1_CD39. 1 hit.
    [Graphical view]
    PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9SPM5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTAKRGIGRH ESLADKIQRH RGIILVISVP IVLIGLVLLL MPGRSISDSV    50
    VEEYSVHNRK GGPNSRGPKN YAVIFDAGSS GSRVHVYCFD QNLDLIPLGN 100
    ELELFLQLKP GLSAYPTDPR QAANSLVSLL DKAEASVPRE LRPKTHVRVG 150
    ATAGLRTLGH DASENILQAV RELLRDRSML KTEANAVTVL DGTQEGSYQW 200
    VTINYLLRNL GKPYSDTVGV VDLGGGSVQM AYAISEEDAA SAPKPLEGED 250
    SYVREMYLKG RKYFLYVHSY LHYGLLAARA EILKVSEDSE NPCIVAGYDG 300
    MYKYGGKEFK APASQSGASL DECRRITINA LKVNDTLCTH MKCTFGGVWN 350
    GGRGGGQKNM FVASFFFDRA AEAGFVDPKQ PVATVRPMDF EKAAKKACSM 400
    KLEEGKSTFP LVEEENLPYL CMDLVYQYTL LIDGFGLEPS QTITLVKKVK 450
    YGDQAVEAAW PLGSAIEAVS SP 472
    Length:472
    Mass (Da):51,599
    Last modified:May 1, 2000 - v1
    Checksum:iFA0C280456D51224
    GO

    Sequence cautioni

    The sequence AED92532.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 201H → N in AFI41206. (PubMed:22430844)Curated
    Sequence conflicti151 – 1511A → V in AAF66599. 1 PublicationCurated
    Sequence conflicti261 – 2611R → Q in AAM98186. (PubMed:14593172)Curated
    Sequence conflicti301 – 3011M → T in AAF66599. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF156783 mRNA. Translation: AAF00612.1.
    AF141671 mRNA. Translation: AAF66599.1.
    JQ937238 mRNA. Translation: AFI41206.1.
    AB012246 Genomic DNA. Translation: BAB09486.1.
    CP002688 Genomic DNA. Translation: AED92532.1. Sequence problems.
    AY099866 mRNA. Translation: AAM20717.1.
    AY140045 mRNA. Translation: AAM98186.1.
    BT000329 mRNA. Translation: AAN15648.1.
    RefSeqiNP_197329.4. NM_121833.6.
    UniGeneiAt.19874.

    Genome annotation databases

    EnsemblPlantsiAT5G18280.1; AT5G18280.1; AT5G18280.
    GeneIDi831946.
    KEGGiath:AT5G18280.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF156783 mRNA. Translation: AAF00612.1 .
    AF141671 mRNA. Translation: AAF66599.1 .
    JQ937238 mRNA. Translation: AFI41206.1 .
    AB012246 Genomic DNA. Translation: BAB09486.1 .
    CP002688 Genomic DNA. Translation: AED92532.1 . Sequence problems.
    AY099866 mRNA. Translation: AAM20717.1 .
    AY140045 mRNA. Translation: AAM98186.1 .
    BT000329 mRNA. Translation: AAN15648.1 .
    RefSeqi NP_197329.4. NM_121833.6.
    UniGenei At.19874.

    3D structure databases

    ProteinModelPortali Q9SPM5.
    SMRi Q9SPM5. Positions 71-463.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9SPM5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G18280.1 ; AT5G18280.1 ; AT5G18280 .
    GeneIDi 831946.
    KEGGi ath:AT5G18280.

    Organism-specific databases

    TAIRi AT5G18280.

    Phylogenomic databases

    InParanoidi Q9SPM5.
    KOi K14641.
    PhylomeDBi Q9SPM5.

    Enzyme and pathway databases

    BioCyci ARA:AT5G18280-MONOMER.
    ARA:GQT-2442-MONOMER.

    Gene expression databases

    Genevestigatori Q9SPM5.

    Family and domain databases

    InterProi IPR000407. GDA1_CD39_NTPase.
    [Graphical view ]
    PANTHERi PTHR11782. PTHR11782. 1 hit.
    Pfami PF01150. GDA1_CD39. 1 hit.
    [Graphical view ]
    PROSITEi PS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Nod factor-binding lectin is a member of a distinct class of apyrases that may be unique to the legumes."
      Roberts N.J., Brigham J., Wu B., Murphy J.B., Volpin H., Phillips D.A., Etzler M.E.
      Mol. Gen. Genet. 262:261-267(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. No-0.
    2. "Molecular and biochemical comparison of two different apyrases from Arabidopsis thaliana."
      Steinebrunner I.A., Jeter C.R., Song C., Roux S.J.
      Plant Physiol. Biochem. 38:913-922(2000)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    3. "Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis."
      Parsons H.T., Christiansen K., Knierim B., Carroll A., Ito J., Batth T.S., Smith-Moritz A.M., Morrison S., McInerney P., Hadi M.Z., Auer M., Mukhopadhyay A., Petzold C.J., Scheller H.V., Loque D., Heazlewood J.L.
      Plant Physiol. 159:12-26(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
      Strain: cv. Landsberg erecta.
    4. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Disruption of apyrases inhibits pollen germination in Arabidopsis."
      Steinebrunner I., Wu J., Sun Y., Corbett A., Roux S.J.
      Plant Physiol. 131:1638-1647(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: cv. Wassilewskija.
    8. "Developmental defects and seedling lethality in apyrase AtAPY1 and AtAPY2 double knockout mutants."
      Wolf C., Hennig M., Romanovicz D., Steinebrunner I.
      Plant Mol. Biol. 64:657-672(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: cv. Wassilewskija.

    Entry informationi

    Entry nameiAPY2_ARATH
    AccessioniPrimary (citable) accession number: Q9SPM5
    Secondary accession number(s): F4JWK7
    , I1VCB1, Q8L704, Q9M7B3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2012
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3