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Q9SPM5

- APY2_ARATH

UniProt

Q9SPM5 - APY2_ARATH

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Protein
Apyrase 2
Gene
APY2, At5g18280, MRG7.24
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Substrate preference is ATP > ADP. Functions with APY1 to reduce extracellular ATP level which is essential for pollen germination and normal plant development. Plays a role in the regulation of stomatal function by modulating extracellular ATP levels in guard cells.3 Publications

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Calcium By similarity.

Kineticsi

  1. KM=30 µM for ATP1 Publication

Vmax=26 µmol/min/mg enzyme

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei195 – 1951Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 8311ATP-binding Inferred
Add
BLAST
Nucleotide bindingi219 – 22911ATP-binding Inferred
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G18280-MONOMER.
ARA:GQT-2442-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Apyrase 2 (EC:3.6.1.5)
Short name:
AtAPY2
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name:
ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 2
Gene namesi
Name:APY2
Ordered Locus Names:At5g18280
ORF Names:MRG7.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G18280.

Subcellular locationi

Golgi apparatus membrane; Single-pass type II membrane protein. Membrane Inferred; Single-pass type II membrane protein Inferred
Note: As cell membrane protein, the functional domain could be at the extracellular side.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei22 – 4221Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini43 – 472430Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions. Apy1 and apy2 double mutant displays developmental defects including the lack of functional root and shoot meristems, and morphogenetic and patterning abnormalities of the cotyledons. Double mutant exhibits a complete inhibition of pollen germination.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini48 – 472425Apyrase 2
PRO_0000419906Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi334 – 3341N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9SPM5.

Expressioni

Tissue specificityi

Expressed in roots, root hairs, root cap, leaves, stems, trichomes, phloem throughout the plant, guard cells, filaments of young stamens, stipules, papillae of stigmas, pollen, pollen tubes and the abscission zone of siliques.3 Publications

Inductioni

By hypertonic stress.

Gene expression databases

GenevestigatoriQ9SPM5.

Structurei

3D structure databases

ProteinModelPortaliQ9SPM5.
SMRiQ9SPM5. Positions 71-463.

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ9SPM5.
KOiK14641.
PhylomeDBiQ9SPM5.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SPM5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTAKRGIGRH ESLADKIQRH RGIILVISVP IVLIGLVLLL MPGRSISDSV    50
VEEYSVHNRK GGPNSRGPKN YAVIFDAGSS GSRVHVYCFD QNLDLIPLGN 100
ELELFLQLKP GLSAYPTDPR QAANSLVSLL DKAEASVPRE LRPKTHVRVG 150
ATAGLRTLGH DASENILQAV RELLRDRSML KTEANAVTVL DGTQEGSYQW 200
VTINYLLRNL GKPYSDTVGV VDLGGGSVQM AYAISEEDAA SAPKPLEGED 250
SYVREMYLKG RKYFLYVHSY LHYGLLAARA EILKVSEDSE NPCIVAGYDG 300
MYKYGGKEFK APASQSGASL DECRRITINA LKVNDTLCTH MKCTFGGVWN 350
GGRGGGQKNM FVASFFFDRA AEAGFVDPKQ PVATVRPMDF EKAAKKACSM 400
KLEEGKSTFP LVEEENLPYL CMDLVYQYTL LIDGFGLEPS QTITLVKKVK 450
YGDQAVEAAW PLGSAIEAVS SP 472
Length:472
Mass (Da):51,599
Last modified:May 1, 2000 - v1
Checksum:iFA0C280456D51224
GO

Sequence cautioni

The sequence AED92532.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201H → N in AFI41206. 1 Publication
Sequence conflicti151 – 1511A → V in AAF66599. 1 Publication
Sequence conflicti261 – 2611R → Q in AAM98186. 1 Publication
Sequence conflicti301 – 3011M → T in AAF66599. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF156783 mRNA. Translation: AAF00612.1.
AF141671 mRNA. Translation: AAF66599.1.
JQ937238 mRNA. Translation: AFI41206.1.
AB012246 Genomic DNA. Translation: BAB09486.1.
CP002688 Genomic DNA. Translation: AED92532.1. Sequence problems.
AY099866 mRNA. Translation: AAM20717.1.
AY140045 mRNA. Translation: AAM98186.1.
BT000329 mRNA. Translation: AAN15648.1.
RefSeqiNP_197329.4. NM_121833.6.
UniGeneiAt.19874.

Genome annotation databases

EnsemblPlantsiAT5G18280.1; AT5G18280.1; AT5G18280.
GeneIDi831946.
KEGGiath:AT5G18280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF156783 mRNA. Translation: AAF00612.1 .
AF141671 mRNA. Translation: AAF66599.1 .
JQ937238 mRNA. Translation: AFI41206.1 .
AB012246 Genomic DNA. Translation: BAB09486.1 .
CP002688 Genomic DNA. Translation: AED92532.1 . Sequence problems.
AY099866 mRNA. Translation: AAM20717.1 .
AY140045 mRNA. Translation: AAM98186.1 .
BT000329 mRNA. Translation: AAN15648.1 .
RefSeqi NP_197329.4. NM_121833.6.
UniGenei At.19874.

3D structure databases

ProteinModelPortali Q9SPM5.
SMRi Q9SPM5. Positions 71-463.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9SPM5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G18280.1 ; AT5G18280.1 ; AT5G18280 .
GeneIDi 831946.
KEGGi ath:AT5G18280.

Organism-specific databases

TAIRi AT5G18280.

Phylogenomic databases

InParanoidi Q9SPM5.
KOi K14641.
PhylomeDBi Q9SPM5.

Enzyme and pathway databases

BioCyci ARA:AT5G18280-MONOMER.
ARA:GQT-2442-MONOMER.

Gene expression databases

Genevestigatori Q9SPM5.

Family and domain databases

InterProi IPR000407. GDA1_CD39_NTPase.
[Graphical view ]
PANTHERi PTHR11782. PTHR11782. 1 hit.
Pfami PF01150. GDA1_CD39. 1 hit.
[Graphical view ]
PROSITEi PS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Nod factor-binding lectin is a member of a distinct class of apyrases that may be unique to the legumes."
    Roberts N.J., Brigham J., Wu B., Murphy J.B., Volpin H., Phillips D.A., Etzler M.E.
    Mol. Gen. Genet. 262:261-267(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. No-0.
  2. "Molecular and biochemical comparison of two different apyrases from Arabidopsis thaliana."
    Steinebrunner I.A., Jeter C.R., Song C., Roux S.J.
    Plant Physiol. Biochem. 38:913-922(2000)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  3. "Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis."
    Parsons H.T., Christiansen K., Knierim B., Carroll A., Ito J., Batth T.S., Smith-Moritz A.M., Morrison S., McInerney P., Hadi M.Z., Auer M., Mukhopadhyay A., Petzold C.J., Scheller H.V., Loque D., Heazlewood J.L.
    Plant Physiol. 159:12-26(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    Strain: cv. Landsberg erecta.
  4. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Disruption of apyrases inhibits pollen germination in Arabidopsis."
    Steinebrunner I., Wu J., Sun Y., Corbett A., Roux S.J.
    Plant Physiol. 131:1638-1647(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    Strain: cv. Wassilewskija.
  8. "Developmental defects and seedling lethality in apyrase AtAPY1 and AtAPY2 double knockout mutants."
    Wolf C., Hennig M., Romanovicz D., Steinebrunner I.
    Plant Mol. Biol. 64:657-672(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    Strain: cv. Wassilewskija.

Entry informationi

Entry nameiAPY2_ARATH
AccessioniPrimary (citable) accession number: Q9SPM5
Secondary accession number(s): F4JWK7
, I1VCB1, Q8L704, Q9M7B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi