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Protein

Formate--tetrahydrofolate ligase

Gene

THFS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi78 – 85ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processOne-carbon metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G50480-MONOMER
ReactomeiR-ATH-196757 Metabolism of folate and pterines
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligase (EC:6.3.4.3)
Alternative name(s):
10-formyletrahydrofolate synthetase
Short name:
FHS
Short name:
FTHFS
Formyltetrahydrofolate synthetase
Gene namesi
Name:THFS
Ordered Locus Names:At1g50480
ORF Names:F11F12.17, F17J6.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G50480
TAIRilocus:2008101 AT1G50480

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001994171 – 634Formate--tetrahydrofolate ligaseAdd BLAST634

Proteomic databases

PaxDbiQ9SPK5
PRIDEiQ9SPK5

2D gel databases

World-2DPAGEi0003:Q9SPK5

PTM databases

iPTMnetiQ9SPK5

Expressioni

Gene expression databases

ExpressionAtlasiQ9SPK5 baseline and differential
GenevisibleiQ9SPK5 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ9SPK5, 1 interactor
STRINGi3702.AT1G50480.1

Structurei

3D structure databases

ProteinModelPortaliQ9SPK5
SMRiQ9SPK5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFVW Eukaryota
COG2759 LUCA
HOGENOMiHOG000040280
InParanoidiQ9SPK5
KOiK01938
OMAiCGEIMTM
OrthoDBiEOG093605CW
PhylomeDBiQ9SPK5

Family and domain databases

CDDicd00477 FTHFS, 1 hit
HAMAPiMF_01543 FTHFS, 1 hit
InterProiView protein in InterPro
IPR000559 Formate_THF_ligase
IPR020628 Formate_THF_ligase_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01268 FTHFS, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00721 FTHFS_1, 1 hit
PS00722 FTHFS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SPK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSTRKLEV VSPVPADIDI ANSVEPLHIS EIAKDLNINP LHYDLYGKYK
60 70 80 90 100
AKVLLSAFDE LQGQEDGYYV VVGGITPTPL GEGKSTTTVG LCQALGAYLD
110 120 130 140 150
KKVVTCLRQP SQGPTFGIKG GAAGGGYSQV IPMDEFNLHL TGDIHAITAS
160 170 180 190 200
NNLLAAAIDT RIFHETSQSD KALFNRLCPP NKEGKRSFSD IMFRRLTKLG
210 220 230 240 250
ISKTSPEELT PEEIKKFARL DIDPASITWR RVMDVNDRFL RKITIGQGPE
260 270 280 290 300
EKGMTRETGF DISVASEIMA VLALTTSLGD MRERLGKMVI GNSKAGDPIT
310 320 330 340 350
ADDLGVGGAL TVLMKDAINP TLMQTLEGTP VLVHAGPFAN IAHGNSSIVA
360 370 380 390 400
DKIALKLVGP GGFVVTEAGF GSDIGTEKFM NIKCRYSGLT PQCAIVVATV
410 420 430 440 450
RALKMHGGGP DVVAGRPLDR AYVSENVSLV EAGCVNLAKH ISNTKAYGVN
460 470 480 490 500
VIVAVNMFAT DTEAELNAVR KFSMDAGAFD AVVCSHHAHS GKGAVDLGIA
510 520 530 540 550
VEKACQNITQ PLRFLYPLDI GIKDKIEAIA KSYGASGVEY SDQAEKQIEM
560 570 580 590 600
YTQQGFSNLP ICMSKTQYSF SHDASKKGAP SGFVLPIRDV RGSIGAGFIY
610 620 630
PLVGTMSTMP GLPTRPCFYE IDIDTETGKV RGLS
Length:634
Mass (Da):67,802
Last modified:May 1, 2000 - v1
Checksum:i5BD9D10603D2FF9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF162279 mRNA Translation: AAD56290.1
AC012561 Genomic DNA Translation: AAF87882.1
AC079279 Genomic DNA Translation: AAG51185.1
CP002684 Genomic DNA Translation: AEE32555.1
AY054637 mRNA Translation: AAK96828.1
AY081549 mRNA Translation: AAM10111.1
PIRiC96541
RefSeqiNP_564571.1, NM_103931.4
UniGeneiAt.21610
At.71081

Genome annotation databases

EnsemblPlantsiAT1G50480.1; AT1G50480.1; AT1G50480
GeneIDi841470
GrameneiAT1G50480.1; AT1G50480.1; AT1G50480
KEGGiath:AT1G50480

Similar proteinsi

Entry informationi

Entry nameiFTHS_ARATH
AccessioniPrimary (citable) accession number: Q9SPK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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