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Q9SPK5 (FTHS_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
10-formyletrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Formyltetrahydrofolate synthetase
Gene names
Name:THFS
Ordered Locus Names:At1g50480
ORF Names:F11F12.17, F17J6.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length634 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 634634Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000199417

Regions

Nucleotide binding78 – 858ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9SPK5 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 5BD9D10603D2FF9A

FASTA63467,802
        10         20         30         40         50         60 
MSSSTRKLEV VSPVPADIDI ANSVEPLHIS EIAKDLNINP LHYDLYGKYK AKVLLSAFDE 

        70         80         90        100        110        120 
LQGQEDGYYV VVGGITPTPL GEGKSTTTVG LCQALGAYLD KKVVTCLRQP SQGPTFGIKG 

       130        140        150        160        170        180 
GAAGGGYSQV IPMDEFNLHL TGDIHAITAS NNLLAAAIDT RIFHETSQSD KALFNRLCPP 

       190        200        210        220        230        240 
NKEGKRSFSD IMFRRLTKLG ISKTSPEELT PEEIKKFARL DIDPASITWR RVMDVNDRFL 

       250        260        270        280        290        300 
RKITIGQGPE EKGMTRETGF DISVASEIMA VLALTTSLGD MRERLGKMVI GNSKAGDPIT 

       310        320        330        340        350        360 
ADDLGVGGAL TVLMKDAINP TLMQTLEGTP VLVHAGPFAN IAHGNSSIVA DKIALKLVGP 

       370        380        390        400        410        420 
GGFVVTEAGF GSDIGTEKFM NIKCRYSGLT PQCAIVVATV RALKMHGGGP DVVAGRPLDR 

       430        440        450        460        470        480 
AYVSENVSLV EAGCVNLAKH ISNTKAYGVN VIVAVNMFAT DTEAELNAVR KFSMDAGAFD 

       490        500        510        520        530        540 
AVVCSHHAHS GKGAVDLGIA VEKACQNITQ PLRFLYPLDI GIKDKIEAIA KSYGASGVEY 

       550        560        570        580        590        600 
SDQAEKQIEM YTQQGFSNLP ICMSKTQYSF SHDASKKGAP SGFVLPIRDV RGSIGAGFIY 

       610        620        630 
PLVGTMSTMP GLPTRPCFYE IDIDTETGKV RGLS 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a cDNA encoding 10-formyletrahydrofolate synthetase from Arabidopsis thaliana."
Skavdahl M., Olson B.J.S.C., Markwell J., Osterman J.C.
Plant Gene Register PGR99-133
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Hypocotyl.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF162279 mRNA. Translation: AAD56290.1.
AC012561 Genomic DNA. Translation: AAF87882.1.
AC079279 Genomic DNA. Translation: AAG51185.1.
CP002684 Genomic DNA. Translation: AEE32555.1.
AY054637 mRNA. Translation: AAK96828.1.
AY081549 mRNA. Translation: AAM10111.1.
PIRC96541.
RefSeqNP_564571.1. NM_103931.3.
UniGeneAt.21610.
At.71081.

3D structure databases

ProteinModelPortalQ9SPK5.
SMRQ9SPK5. Positions 17-633.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G50480.1-P.

2D gel databases

World-2DPAGE0003:Q9SPK5.

Proteomic databases

PaxDbQ9SPK5.
PRIDEQ9SPK5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G50480.1; AT1G50480.1; AT1G50480.
GeneID841470.
KEGGath:AT1G50480.

Organism-specific databases

GeneFarm4946.
TAIRAT1G50480.

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
InParanoidQ9SPK5.
KOK01938.
OMAHEYLDEN.
PhylomeDBQ9SPK5.
ProtClustDBPLN02759.

Enzyme and pathway databases

BioCycARA:AT1G50480-MONOMER.
UniPathwayUPA00193.

Gene expression databases

GenevestigatorQ9SPK5.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9SPK5.

Entry information

Entry nameFTHS_ARATH
AccessionPrimary (citable) accession number: Q9SPK5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names