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Q9SPK5

- FTHS_ARATH

UniProt

Q9SPK5 - FTHS_ARATH

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Protein
Formate--tetrahydrofolate ligase
Gene
THFS, At1g50480, F11F12.17, F17J6.2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 858ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. copper ion binding Source: TAIR
  3. formate-tetrahydrofolate ligase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. response to cadmium ion Source: TAIR
  3. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G50480-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligase (EC:6.3.4.3)
Alternative name(s):
10-formyletrahydrofolate synthetase
Short name:
FHS
Short name:
FTHFS
Formyltetrahydrofolate synthetase
Gene namesi
Name:THFS
Ordered Locus Names:At1g50480
ORF Names:F11F12.17, F17J6.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G50480.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. chloroplast Source: TAIR
  3. cytosol Source: TAIR
  4. plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 634634Formate--tetrahydrofolate ligaseUniRule annotation
PRO_0000199417Add
BLAST

Proteomic databases

PaxDbiQ9SPK5.
PRIDEiQ9SPK5.

2D gel databases

World-2DPAGE0003:Q9SPK5.

Expressioni

Gene expression databases

GenevestigatoriQ9SPK5.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

IntActiQ9SPK5. 1 interaction.
STRINGi3702.AT1G50480.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SPK5.
SMRiQ9SPK5. Positions 14-633.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
InParanoidiQ9SPK5.
KOiK01938.
OMAiHEYLDEN.
PhylomeDBiQ9SPK5.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SPK5-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSTRKLEV VSPVPADIDI ANSVEPLHIS EIAKDLNINP LHYDLYGKYK    50
AKVLLSAFDE LQGQEDGYYV VVGGITPTPL GEGKSTTTVG LCQALGAYLD 100
KKVVTCLRQP SQGPTFGIKG GAAGGGYSQV IPMDEFNLHL TGDIHAITAS 150
NNLLAAAIDT RIFHETSQSD KALFNRLCPP NKEGKRSFSD IMFRRLTKLG 200
ISKTSPEELT PEEIKKFARL DIDPASITWR RVMDVNDRFL RKITIGQGPE 250
EKGMTRETGF DISVASEIMA VLALTTSLGD MRERLGKMVI GNSKAGDPIT 300
ADDLGVGGAL TVLMKDAINP TLMQTLEGTP VLVHAGPFAN IAHGNSSIVA 350
DKIALKLVGP GGFVVTEAGF GSDIGTEKFM NIKCRYSGLT PQCAIVVATV 400
RALKMHGGGP DVVAGRPLDR AYVSENVSLV EAGCVNLAKH ISNTKAYGVN 450
VIVAVNMFAT DTEAELNAVR KFSMDAGAFD AVVCSHHAHS GKGAVDLGIA 500
VEKACQNITQ PLRFLYPLDI GIKDKIEAIA KSYGASGVEY SDQAEKQIEM 550
YTQQGFSNLP ICMSKTQYSF SHDASKKGAP SGFVLPIRDV RGSIGAGFIY 600
PLVGTMSTMP GLPTRPCFYE IDIDTETGKV RGLS 634
Length:634
Mass (Da):67,802
Last modified:May 1, 2000 - v1
Checksum:i5BD9D10603D2FF9A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF162279 mRNA. Translation: AAD56290.1.
AC012561 Genomic DNA. Translation: AAF87882.1.
AC079279 Genomic DNA. Translation: AAG51185.1.
CP002684 Genomic DNA. Translation: AEE32555.1.
AY054637 mRNA. Translation: AAK96828.1.
AY081549 mRNA. Translation: AAM10111.1.
PIRiC96541.
RefSeqiNP_564571.1. NM_103931.3.
UniGeneiAt.21610.
At.71081.

Genome annotation databases

EnsemblPlantsiAT1G50480.1; AT1G50480.1; AT1G50480.
GeneIDi841470.
KEGGiath:AT1G50480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF162279 mRNA. Translation: AAD56290.1 .
AC012561 Genomic DNA. Translation: AAF87882.1 .
AC079279 Genomic DNA. Translation: AAG51185.1 .
CP002684 Genomic DNA. Translation: AEE32555.1 .
AY054637 mRNA. Translation: AAK96828.1 .
AY081549 mRNA. Translation: AAM10111.1 .
PIRi C96541.
RefSeqi NP_564571.1. NM_103931.3.
UniGenei At.21610.
At.71081.

3D structure databases

ProteinModelPortali Q9SPK5.
SMRi Q9SPK5. Positions 14-633.
ModBasei Search...

Protein-protein interaction databases

IntActi Q9SPK5. 1 interaction.
STRINGi 3702.AT1G50480.1-P.

2D gel databases

World-2DPAGE 0003:Q9SPK5.

Proteomic databases

PaxDbi Q9SPK5.
PRIDEi Q9SPK5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G50480.1 ; AT1G50480.1 ; AT1G50480 .
GeneIDi 841470.
KEGGi ath:AT1G50480.

Organism-specific databases

GeneFarmi 4946.
TAIRi AT1G50480.

Phylogenomic databases

eggNOGi COG2759.
HOGENOMi HOG000040280.
InParanoidi Q9SPK5.
KOi K01938.
OMAi HEYLDEN.
PhylomeDBi Q9SPK5.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci ARA:AT1G50480-MONOMER.

Miscellaneous databases

PROi Q9SPK5.

Gene expression databases

Genevestigatori Q9SPK5.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_01543. FTHFS.
InterProi IPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF01268. FTHFS. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a cDNA encoding 10-formyletrahydrofolate synthetase from Arabidopsis thaliana."
    Skavdahl M., Olson B.J.S.C., Markwell J., Osterman J.C.
    Plant Gene Register PGR99-133
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Hypocotyl.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiFTHS_ARATH
AccessioniPrimary (citable) accession number: Q9SPK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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