Reviewed, UniProtKB/Swiss-Prot Q9SPK5 (FTHS_ARATH)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase
    10-formyletrahydrofolate synthetase
      Short name=FHS
      Short name=FTHFS

Gene names

Name:	THFS
Ordered Locus Names:	At1g50480
ORF Names:	F11F12.17, F17J6.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from direct assay. Source: TAIR formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 634	634	Formate--tetrahydrofolate ligase		PRO_0000199417
Regions
	Nucleotide binding	78 – 85	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SPK5-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 5BD9D10603D2FF9A	FASTA	634	67,802
	10 20 30 40 50 60 MSSSTRKLEV VSPVPADIDI ANSVEPLHIS EIAKDLNINP LHYDLYGKYK AKVLLSAFDE 70 80 90 100 110 120 LQGQEDGYYV VVGGITPTPL GEGKSTTTVG LCQALGAYLD KKVVTCLRQP SQGPTFGIKG 130 140 150 160 170 180 GAAGGGYSQV IPMDEFNLHL TGDIHAITAS NNLLAAAIDT RIFHETSQSD KALFNRLCPP 190 200 210 220 230 240 NKEGKRSFSD IMFRRLTKLG ISKTSPEELT PEEIKKFARL DIDPASITWR RVMDVNDRFL 250 260 270 280 290 300 RKITIGQGPE EKGMTRETGF DISVASEIMA VLALTTSLGD MRERLGKMVI GNSKAGDPIT 310 320 330 340 350 360 ADDLGVGGAL TVLMKDAINP TLMQTLEGTP VLVHAGPFAN IAHGNSSIVA DKIALKLVGP 370 380 390 400 410 420 GGFVVTEAGF GSDIGTEKFM NIKCRYSGLT PQCAIVVATV RALKMHGGGP DVVAGRPLDR 430 440 450 460 470 480 AYVSENVSLV EAGCVNLAKH ISNTKAYGVN VIVAVNMFAT DTEAELNAVR KFSMDAGAFD 490 500 510 520 530 540 AVVCSHHAHS GKGAVDLGIA VEKACQNITQ PLRFLYPLDI GIKDKIEAIA KSYGASGVEY 550 560 570 580 590 600 SDQAEKQIEM YTQQGFSNLP ICMSKTQYSF SHDASKKGAP SGFVLPIRDV RGSIGAGFIY 610 620 630 PLVGTMSTMP GLPTRPCFYE IDIDTETGKV RGLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a cDNA encoding 10-formyletrahydrofolate synthetase from Arabidopsis thaliana." Skavdahl M., Olson B.J.S.C., Markwell J., Osterman J.C. Plant Gene Register PGR99-133 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Hypocotyl.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF162279 mRNA. Translation: AAD56290.1. AC012561 Genomic DNA. Translation: AAF87882.1. AC079279 Genomic DNA. Translation: AAG51185.1. AY054637 mRNA. Translation: AAK96828.1. AY081549 mRNA. Translation: AAM10111.1.
IPI	IPI00540904.
PIR	C96541.
RefSeq	NP_564571.1.
UniGene	At.21610
3D structure databases
HSSP	HSSP built from PDB template 1EG7 based on UniProtKB P21164.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SPK5.
Proteomic databases
PRIDE	Q9SPK5.
Genome annotation databases
GeneID	841470.
GenomeReviews	Gene locus AT1G50480 in contig CT485782_GR.
KEGG	ath:AT1G50480.
NMPDR	fig|3702.1.peg.4522.
Organism-specific databases
GeneFarm	4946.
TAIR	At1g50480.
Phylogenomic databases
OMA	KAEVYTK.
Enzyme and pathway databases
BRENDA	6.3.4.3. 302.
Gene expression databases
ArrayExpress	Q9SPK5.
Genevestigator	Q9SPK5.
GermOnline	AT1G50480. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000559. For_THF_ligase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_ARATH

Accession

Primary (citable) accession number: Q9SPK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)