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Q9SPK5

- FTHS_ARATH

UniProt

Q9SPK5 - FTHS_ARATH

Protein

Formate--tetrahydrofolate ligase

Gene

THFS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi78 – 858ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. copper ion binding Source: TAIR
    3. formate-tetrahydrofolate ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. folic acid-containing compound biosynthetic process Source: InterPro
    2. response to cadmium ion Source: TAIR
    3. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G50480-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Formate--tetrahydrofolate ligase (EC:6.3.4.3)
    Alternative name(s):
    10-formyletrahydrofolate synthetase
    Short name:
    FHS
    Short name:
    FTHFS
    Formyltetrahydrofolate synthetase
    Gene namesi
    Name:THFS
    Ordered Locus Names:At1g50480
    ORF Names:F11F12.17, F17J6.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G50480.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. chloroplast Source: TAIR
    3. cytosol Source: TAIR
    4. plasma membrane Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 634634Formate--tetrahydrofolate ligasePRO_0000199417Add
    BLAST

    Proteomic databases

    PaxDbiQ9SPK5.
    PRIDEiQ9SPK5.

    2D gel databases

    World-2DPAGE0003:Q9SPK5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9SPK5.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiQ9SPK5. 1 interaction.
    STRINGi3702.AT1G50480.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SPK5.
    SMRiQ9SPK5. Positions 14-633.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2759.
    HOGENOMiHOG000040280.
    InParanoidiQ9SPK5.
    KOiK01938.
    OMAiHEYLDEN.
    PhylomeDBiQ9SPK5.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    HAMAPiMF_01543. FTHFS.
    InterProiIPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01268. FTHFS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SPK5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSTRKLEV VSPVPADIDI ANSVEPLHIS EIAKDLNINP LHYDLYGKYK    50
    AKVLLSAFDE LQGQEDGYYV VVGGITPTPL GEGKSTTTVG LCQALGAYLD 100
    KKVVTCLRQP SQGPTFGIKG GAAGGGYSQV IPMDEFNLHL TGDIHAITAS 150
    NNLLAAAIDT RIFHETSQSD KALFNRLCPP NKEGKRSFSD IMFRRLTKLG 200
    ISKTSPEELT PEEIKKFARL DIDPASITWR RVMDVNDRFL RKITIGQGPE 250
    EKGMTRETGF DISVASEIMA VLALTTSLGD MRERLGKMVI GNSKAGDPIT 300
    ADDLGVGGAL TVLMKDAINP TLMQTLEGTP VLVHAGPFAN IAHGNSSIVA 350
    DKIALKLVGP GGFVVTEAGF GSDIGTEKFM NIKCRYSGLT PQCAIVVATV 400
    RALKMHGGGP DVVAGRPLDR AYVSENVSLV EAGCVNLAKH ISNTKAYGVN 450
    VIVAVNMFAT DTEAELNAVR KFSMDAGAFD AVVCSHHAHS GKGAVDLGIA 500
    VEKACQNITQ PLRFLYPLDI GIKDKIEAIA KSYGASGVEY SDQAEKQIEM 550
    YTQQGFSNLP ICMSKTQYSF SHDASKKGAP SGFVLPIRDV RGSIGAGFIY 600
    PLVGTMSTMP GLPTRPCFYE IDIDTETGKV RGLS 634
    Length:634
    Mass (Da):67,802
    Last modified:May 1, 2000 - v1
    Checksum:i5BD9D10603D2FF9A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF162279 mRNA. Translation: AAD56290.1.
    AC012561 Genomic DNA. Translation: AAF87882.1.
    AC079279 Genomic DNA. Translation: AAG51185.1.
    CP002684 Genomic DNA. Translation: AEE32555.1.
    AY054637 mRNA. Translation: AAK96828.1.
    AY081549 mRNA. Translation: AAM10111.1.
    PIRiC96541.
    RefSeqiNP_564571.1. NM_103931.3.
    UniGeneiAt.21610.
    At.71081.

    Genome annotation databases

    EnsemblPlantsiAT1G50480.1; AT1G50480.1; AT1G50480.
    GeneIDi841470.
    KEGGiath:AT1G50480.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF162279 mRNA. Translation: AAD56290.1 .
    AC012561 Genomic DNA. Translation: AAF87882.1 .
    AC079279 Genomic DNA. Translation: AAG51185.1 .
    CP002684 Genomic DNA. Translation: AEE32555.1 .
    AY054637 mRNA. Translation: AAK96828.1 .
    AY081549 mRNA. Translation: AAM10111.1 .
    PIRi C96541.
    RefSeqi NP_564571.1. NM_103931.3.
    UniGenei At.21610.
    At.71081.

    3D structure databases

    ProteinModelPortali Q9SPK5.
    SMRi Q9SPK5. Positions 14-633.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9SPK5. 1 interaction.
    STRINGi 3702.AT1G50480.1-P.

    2D gel databases

    World-2DPAGE 0003:Q9SPK5.

    Proteomic databases

    PaxDbi Q9SPK5.
    PRIDEi Q9SPK5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G50480.1 ; AT1G50480.1 ; AT1G50480 .
    GeneIDi 841470.
    KEGGi ath:AT1G50480.

    Organism-specific databases

    GeneFarmi 4946.
    TAIRi AT1G50480.

    Phylogenomic databases

    eggNOGi COG2759.
    HOGENOMi HOG000040280.
    InParanoidi Q9SPK5.
    KOi K01938.
    OMAi HEYLDEN.
    PhylomeDBi Q9SPK5.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci ARA:AT1G50480-MONOMER.

    Miscellaneous databases

    PROi Q9SPK5.

    Gene expression databases

    Genevestigatori Q9SPK5.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    HAMAPi MF_01543. FTHFS.
    InterProi IPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF01268. FTHFS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a cDNA encoding 10-formyletrahydrofolate synthetase from Arabidopsis thaliana."
      Skavdahl M., Olson B.J.S.C., Markwell J., Osterman J.C.
      Plant Gene Register PGR99-133
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
      Tissue: Hypocotyl.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiFTHS_ARATH
    AccessioniPrimary (citable) accession number: Q9SPK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3