Q9SPE5 (SNAG_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-soluble NSF attachment protein
Short name=Gamma-SNAP

Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein gamma

Gene names

Name:	GSNAP
Ordered Locus Names:	At4g20410
ORF Names:	F9F13.60

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. Binds to SNARE complex and then recruits NSF to disassemble it By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the SNAP family.
Sequence caution	The sequence CAB45807.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79041.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Gamma-soluble NSF attachment protein		PRO_0000219070
Experimental info
	Sequence conflict	45 – 46	2	NK → KN in AAM63730. Ref.4
	Sequence conflict	129	1	A → S in AAM63730. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9SPE5 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 3EECE3B64D038BAD	FASTA	291	32,360
	10 20 30 40 50 60 MSSDPDKMMS KADKMTKLTL TRWSADWRGA TELYEQAANG FRASNKYEKA KVALEKASKG 70 80 90 100 110 120 QEMQASPWDA AKHMESAAAL AQKLSIWNEV ADFYRKASEL YVECGRAQPA SDALGKAARA 130 140 150 160 170 180 LEDVKPDDAI QLYTDACEIL EEDGRDQMAF DLYRACANVY IKLEKFTDAA TFFLRLGVAA 190 200 210 220 230 240 DKCDATNSQC KAYLSAIILY LYAHDLQQAE KCYNDCSQID AFLKSDQSRS ASRLLTAYNE 250 260 270 280 290 GDIEEIKKVA SASTVSNLDH MIIKLARKLP TGDVTAIQMN TNDDLDEDDL T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional and molecular identification of novel members of the ubiquitous membrane fusion proteins alpha- and gamma-SNAP (soluble N-ethylmaleimide-sensitive factor-attachment proteins) families in Dictyostelium discoideum." Weidenhaupt M., Bruckert F., Louwagie M., Garin J., Satre M. Eur. J. Biochem. 267:2062-2070(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF177990 mRNA. Translation: AAF01285.1. AL080253 Genomic DNA. Translation: CAB45807.1. Sequence problems. AL161553 Genomic DNA. Translation: CAB79041.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE84326.1. AY086673 mRNA. Translation: AAM63730.1.
IPI	IPI00548391.
RefSeq	NP_567600.1. NM_118160.4.
UniGene	At.22040.
3D structure databases
ProteinModelPortal	Q9SPE5.
SMR	Q9SPE5. Positions 3-269.
ModBase	Search...
Proteomic databases
PaxDb	Q9SPE5.
PRIDE	Q9SPE5.
Protocols and materials databases
DNASU	827789.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G20410.1; AT4G20410.1; AT4G20410.
GeneID	827789.
KEGG	ath:AT4G20410.
Organism-specific databases
TAIR	At4g20410.
Phylogenomic databases
eggNOG	NOG296973.
HOGENOM	HOG000006275.
InParanoid	Q9SPE5.
OMA	RAQPASD.
PhylomeDB	Q9SPE5.
ProtClustDB	CLSN2689525.
Gene expression databases
ArrayExpress	Q9SPE5.
Genevestigator	Q9SPE5.
GermOnline	AT4G20410. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR000744. NSF_attach. IPR011990. TPR-like_helical. [Graphical view]
PANTHER	PTHR13768. PTHR13768. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SNAG_ARATH

Accession

Primary (citable) accession number: Q9SPE5
Secondary accession number(s): Q9SUN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program