Q9SNY3 (GMD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GDP-mannose 4,6 dehydratase 1
EC=4.2.1.47

Alternative name(s):
GDP-D-mannose dehydratase 1
Short name=GMD 1

Gene names

Name:	GMD1
Ordered Locus Names:	At5g66280
ORF Names:	K1L20.6

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conversion of GDP-D-mannose to GDP-4-keto-6-D-deoxymannose By similarity.
Catalytic activity	GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O.
Cofactor	NADP By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.
Subunit structure	Homotetramer By similarity.
Tissue specificity	Expressed in roots and pollen.
Sequence similarities	Belongs to the GDP-mannose 4,6-dehydratase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GDP-mannose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GDP-mannose 4,6-dehydratase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	GDP-mannose 4,6 dehydratase 1		PRO_0000201710
Sites
	Active site	150	1	By similarity
	Active site	152	1	Nucleophile By similarity
	Active site	173	1	Nucleophile By similarity
	Binding site	177	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SNY3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 3ADC7AE7394FAD81	FASTA	361	40,850
	10 20 30 40 50 60 MASRSLNGDS DIVKPRKIAL VTGITGQDGS YLTEFLLEKG YEVHGLIRRS SNFNTQRLNH 70 80 90 100 110 120 IYVDPHNVNK ALMKLHYGDL SDASSLRRWL DVIKPDEVYN LAAQSHVAVS FEIPDYTADV 130 140 150 160 170 180 VATGALRLLE AVRSHNIDNG RAIKYYQAGS SEMFGSTPPP QSETTPFHPR SPYAASKCAA 190 200 210 220 230 240 HWYTVNYREA YGLYACNGIL FNHESPRRGE NFVTRKITRA LGRIKVGLQT KLFLGNIQAS 250 260 270 280 290 300 RDWGFAGDYV EAMWLMLQQE KPDDYVVATE ESHTVKEFLD VSFGYVGLNW KDHVEIDKRY 310 320 330 340 350 360 FRPTEVDNLK GDASKAKEML GWKPKVGFEK LVKMMVDEDL ELAKREKVLA DAGYMDAQQQ P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The GMD1 gene of Arabidopsis thaliana encodes an isoform of GDP-D-mannose 4,6-dehydratase that is expressed in roots and pollen." Bonin C.P., Reiter W.-D. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF195140 mRNA. Translation: AAF07199.1. AB022211 Genomic DNA. Translation: BAB10707.1. CP002688 Genomic DNA. Translation: AED98194.1.
IPI	IPI00517362.
RefSeq	NP_201429.1. NM_126026.3.
UniGene	At.46822.
3D structure databases
ProteinModelPortal	Q9SNY3.
SMR	Q9SNY3. Positions 16-356.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SNY3. 1 interaction.
STRING	Q9SNY3.
Proteomic databases
PRIDE	Q9SNY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G66280.1; AT5G66280.1; AT5G66280.
GeneID	836760.
GenomeReviews	Gene locus AT5G66280 in contig BA000015_GR.
KEGG	ath:AT5G66280.
NMPDR	fig|3702.1.peg.28730.
Organism-specific databases
TAIR	At5g66280.
Phylogenomic databases
GeneTree	EPGT00050000006545.
HOGENOM	HBG755066.
InParanoid	Q9SNY3.
OMA	FNHESEL.
PhylomeDB	Q9SNY3.
ProtClustDB	PLN02653.
Gene expression databases
ArrayExpress	Q9SNY3.
Genevestigator	Q9SNY3.
GermOnline	AT5G66280. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR006368. GDP_Man_deHydtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01711.
PANTHER	PTHR10366:SF32. GDP_mann_dehyd. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01472. Gmd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GMD1_ARATH

Accession

Primary (citable) accession number: Q9SNY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program