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Protein

U-box domain-containing protein 13

Gene

PUB13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as an E3 ubiquitin ligase.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • transmembrane receptor protein serine/threonine kinase binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • negative regulation of apoptotic process Source: CACAO
  • protein ubiquitination Source: TAIR
  • regulation of photoperiodism, flowering Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:GQT-1580-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
U-box domain-containing protein 13 (EC:6.3.2.-)
Alternative name(s):
Plant U-box protein 13
Gene namesi
Name:PUB13
Ordered Locus Names:At3g46510
ORF Names:F12A12.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G46510.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: TAIR
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 660660U-box domain-containing protein 13PRO_0000322158Add
BLAST

Post-translational modificationi

Phosphorylated by SD1-6 and SD1-7.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SNC6.
PRIDEiQ9SNC6.

PTM databases

iPTMnetiQ9SNC6.

Expressioni

Gene expression databases

GenevisibleiQ9SNC6. AT.

Interactioni

Subunit structurei

Binds to SD11, SD16, SD17, SD18, SD113, SD129 and SD25.

GO - Molecular functioni

  • transmembrane receptor protein serine/threonine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi9124. 7 interactions.
STRINGi3702.AT3G46510.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SNC6.
SMRiQ9SNC6. Positions 254-329, 350-627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini255 – 32975U-boxAdd
BLAST
Repeati384 – 42340ARM 1Add
BLAST
Repeati425 – 46440ARM 2Add
BLAST
Repeati466 – 50540ARM 3Add
BLAST
Repeati507 – 54640ARM 4Add
BLAST
Repeati548 – 58740ARM 5Add
BLAST

Sequence similaritiesi

Contains 5 ARM repeats.PROSITE-ProRule annotation
Contains 1 U-box domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITMM. Eukaryota.
ENOG4110352. LUCA.
HOGENOMiHOG000238412.
InParanoidiQ9SNC6.
OMAiCIEKWIE.
PhylomeDBiQ9SNC6.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00514. Arm. 2 hits.
PF04564. U-box. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 6 hits.
SM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 2 hits.
PS51698. U_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SNC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEEKASAAQ SLIDVVNEIA AISDYRITVK KLCYNLARRL KLLVPMFEEI
60 70 80 90 100
RESNEPISED TLKTLMNLKE AMCSAKDYLK FCSQGSKIYL VMEREQVTSK
110 120 130 140 150
LMEVSVKLEQ SLSQIPYEEL DISDEVREQV ELVLSQFRRA KGRVDVSDDE
160 170 180 190 200
LYEDLQSLCN KSSDVDAYQP VLERVAKKLH LMEIPDLAQE SVALHEMVAS
210 220 230 240 250
SGGDVGENIE EMAMVLKMIK DFVQTEDDNG EEQKVGVNSR SNGQTSTAAS
260 270 280 290 300
QKIPVIPDDF RCPISLEMMR DPVIVSSGQT YERTCIEKWI EGGHSTCPKT
310 320 330 340 350
QQALTSTTLT PNYVLRSLIA QWCEANDIEP PKPPSSLRPR KVSSFSSPAE
360 370 380 390 400
ANKIEDLMWR LAYGNPEDQR SAAGEIRLLA KRNADNRVAI AEAGAIPLLV
410 420 430 440 450
GLLSTPDSRI QEHSVTALLN LSICENNKGA IVSAGAIPGI VQVLKKGSME
460 470 480 490 500
ARENAAATLF SLSVIDENKV TIGALGAIPP LVVLLNEGTQ RGKKDAATAL
510 520 530 540 550
FNLCIYQGNK GKAIRAGVIP TLTRLLTEPG SGMVDEALAI LAILSSHPEG
560 570 580 590 600
KAIIGSSDAV PSLVEFIRTG SPRNRENAAA VLVHLCSGDP QHLVEAQKLG
610 620 630 640 650
LMGPLIDLAG NGTDRGKRKA AQLLERISRL AEQQKETAVS QPEEEAEPTH
660
PESTTEAADT
Length:660
Mass (Da):71,966
Last modified:May 1, 2000 - v1
Checksum:i2742A7D472B73BD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133314 Genomic DNA. Translation: CAB62321.1.
CP002686 Genomic DNA. Translation: AEE78167.1.
AY042791 mRNA. Translation: AAK68731.1.
AY128813 mRNA. Translation: AAM91213.1.
PIRiT45588.
RefSeqiNP_190235.1. NM_114518.3.
UniGeneiAt.23070.

Genome annotation databases

EnsemblPlantsiAT3G46510.1; AT3G46510.1; AT3G46510.
GeneIDi823804.
GrameneiAT3G46510.1; AT3G46510.1; AT3G46510.
KEGGiath:AT3G46510.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133314 Genomic DNA. Translation: CAB62321.1.
CP002686 Genomic DNA. Translation: AEE78167.1.
AY042791 mRNA. Translation: AAK68731.1.
AY128813 mRNA. Translation: AAM91213.1.
PIRiT45588.
RefSeqiNP_190235.1. NM_114518.3.
UniGeneiAt.23070.

3D structure databases

ProteinModelPortaliQ9SNC6.
SMRiQ9SNC6. Positions 254-329, 350-627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9124. 7 interactions.
STRINGi3702.AT3G46510.1.

PTM databases

iPTMnetiQ9SNC6.

Proteomic databases

PaxDbiQ9SNC6.
PRIDEiQ9SNC6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G46510.1; AT3G46510.1; AT3G46510.
GeneIDi823804.
GrameneiAT3G46510.1; AT3G46510.1; AT3G46510.
KEGGiath:AT3G46510.

Organism-specific databases

TAIRiAT3G46510.

Phylogenomic databases

eggNOGiENOG410ITMM. Eukaryota.
ENOG4110352. LUCA.
HOGENOMiHOG000238412.
InParanoidiQ9SNC6.
OMAiCIEKWIE.
PhylomeDBiQ9SNC6.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:GQT-1580-MONOMER.

Miscellaneous databases

PROiQ9SNC6.

Gene expression databases

GenevisibleiQ9SNC6. AT.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00514. Arm. 2 hits.
PF04564. U-box. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 6 hits.
SM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 2 hits.
PS51698. U_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
  5. "A large complement of the predicted Arabidopsis ARM repeat proteins are members of the U-box E3 ubiquitin ligase family."
    Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.
    Plant Physiol. 134:59-66(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION.
  6. "Interactions between the S-domain receptor kinases and AtPUB-ARM E3 ubiquitin ligases suggest a conserved signaling pathway in Arabidopsis."
    Samuel M.A., Mudgil Y., Salt J.N., Delmas F., Ramachandran S., Chilelli A., Goring D.R.
    Plant Physiol. 147:2084-2095(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SD11; SD16; SD17; SD18; SD113; SD129 AND SD25, PHOSPHORYLATION, SUBCELLULAR LOCATION.
  7. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUB13_ARATH
AccessioniPrimary (citable) accession number: Q9SNC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 1, 2000
Last modified: February 17, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.