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Protein

Malate dehydrogenase, chloroplastic

Gene

At3g47520

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei115 – 1151NADBy similarity
Binding sitei162 – 1621SubstratePROSITE-ProRule annotation
Binding sitei168 – 1681SubstratePROSITE-ProRule annotation
Binding sitei175 – 1751NADBy similarity
Binding sitei200 – 2001SubstratePROSITE-ProRule annotation
Binding sitei234 – 2341SubstratePROSITE-ProRule annotation
Active sitei258 – 2581Proton acceptorBy similarity
Binding sitei309 – 3091NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi89 – 957NADBy similarity
Nucleotide bindingi198 – 2003NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: TAIR
  2. NAD(P)+ transhydrogenase activity Source: TAIR

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. embryo development ending in seed dormancy Source: TAIR
  3. malate metabolic process Source: InterPro
  4. response to cold Source: TAIR
  5. response to cytokinin Source: TAIR
  6. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT3G47520-MONOMER.
MetaCyc:AT3G47520-MONOMER.
ReactomeiREACT_239253. Gluconeogenesis.
REACT_242439. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, chloroplastic (EC:1.1.1.37)
Alternative name(s):
pNAD-MDH
Gene namesi
Ordered Locus Names:At3g47520
ORF Names:F1P2.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G47520.

Subcellular locationi

Plastidchloroplast stroma 2 Publications

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. chloroplast Source: TAIR
  3. chloroplast envelope Source: TAIR
  4. chloroplast stroma Source: TAIR
  5. membrane Source: TAIR
  6. mitochondrion Source: TAIR
  7. plastid Source: TAIR
  8. plastid stroma Source: TAIR
  9. stromule Source: TAIR
  10. vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8080ChloroplastSequence AnalysisAdd
BLAST
Chaini81 – 403323Malate dehydrogenase, chloroplasticPRO_0000224149Add
BLAST

Proteomic databases

PaxDbiQ9SN86.
PRIDEiQ9SN86.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SN86. baseline and differential.
GenevestigatoriQ9SN86.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi9226. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9SN86.
SMRiQ9SN86. Positions 83-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
InParanoidiQ9SN86.
KOiK00026.
OMAiLSHINSK.
PhylomeDBiQ9SN86.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SN86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATATSASLF STVSSSYSKA SSIPHSRLQS VKFNSVPSFT GLKSTSLISG
60 70 80 90 100
SDSSSLAKTL RGSVTKAQTS DKKPYGFKIN ASYKVAVLGA AGGIGQPLSL
110 120 130 140 150
LIKMSPLVST LHLYDIANVK GVAADLSHCN TPSQVRDFTG PSELADCLKD
160 170 180 190 200
VNVVVIPAGV PRKPGMTRDD LFNINANIVK TLVEAVAENC PNAFIHIISN
210 220 230 240 250
PVNSTVPIAA EVLKKKGVYD PKKLFGVTTL DVVRANTFVS QKKNLKLIDV
260 270 280 290 300
DVPVIGGHAG ITILPLLSKT KPSVNFTDEE IQELTVRIQN AGTEVVDAKA
310 320 330 340 350
GAGSATLSMA YAAARFVESS LRALDGDGDV YECSFVESTL TDLPFFASRV
360 370 380 390 400
KIGKNGLEAV IESDLQGLTE YEQKALEALK VELKASIDKG VAFANKPAAA

AAN
Length:403
Mass (Da):42,406
Last modified:May 1, 2000 - v1
Checksum:i65B54DE66392D229
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571A → T in CAA74320 (PubMed:9774405).Curated
Sequence conflicti66 – 661K → N in CAA74320 (PubMed:9774405).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13987 mRNA. Translation: CAA74320.1.
AL132955 Genomic DNA. Translation: CAB61978.1.
CP002686 Genomic DNA. Translation: AEE78292.1.
AY128281 mRNA. Translation: AAM91090.1.
BT000621 mRNA. Translation: AAN18188.1.
PIRiT45712.
T51862.
RefSeqiNP_190336.1. NM_114620.2.
UniGeneiAt.20474.

Genome annotation databases

EnsemblPlantsiAT3G47520.1; AT3G47520.1; AT3G47520.
GeneIDi823906.
KEGGiath:AT3G47520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13987 mRNA. Translation: CAA74320.1.
AL132955 Genomic DNA. Translation: CAB61978.1.
CP002686 Genomic DNA. Translation: AEE78292.1.
AY128281 mRNA. Translation: AAM91090.1.
BT000621 mRNA. Translation: AAN18188.1.
PIRiT45712.
T51862.
RefSeqiNP_190336.1. NM_114620.2.
UniGeneiAt.20474.

3D structure databases

ProteinModelPortaliQ9SN86.
SMRiQ9SN86. Positions 83-393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9226. 1 interaction.

Proteomic databases

PaxDbiQ9SN86.
PRIDEiQ9SN86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G47520.1; AT3G47520.1; AT3G47520.
GeneIDi823906.
KEGGiath:AT3G47520.

Organism-specific databases

TAIRiAT3G47520.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
InParanoidiQ9SN86.
KOiK00026.
OMAiLSHINSK.
PhylomeDBiQ9SN86.

Enzyme and pathway databases

BioCyciARA:AT3G47520-MONOMER.
MetaCyc:AT3G47520-MONOMER.
ReactomeiREACT_239253. Gluconeogenesis.
REACT_242439. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiQ9SN86.

Gene expression databases

ExpressionAtlasiQ9SN86. baseline and differential.
GenevestigatoriQ9SN86.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel, non-redox-regulated NAD-dependent malate dehydrogenase from chloroplasts of Arabidopsis thaliana L."
    Berkemeyer M., Scheibe R., Ocheretina O.
    J. Biol. Chem. 273:27927-27933(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
    Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
    Mol. Cell. Proteomics 2:325-345(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: cv. Wassilewskija.

Entry informationi

Entry nameiMDHP_ARATH
AccessioniPrimary (citable) accession number: Q9SN86
Secondary accession number(s): O81844
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: May 1, 2000
Last modified: February 4, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.