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Reviewed, UniProtKB/Swiss-Prot Q9SN86 (MDHP_ARATH)

Last modified June 16, 2009. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Malate dehydrogenase, chloroplastic
    EC=1.1.1.37
Alternative name(s):
    pNAD-MDH
Gene names
Ordered Locus Names: At3g47520
ORF Names: F1P2.70
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastid › chloroplast stroma. Ref.1 Ref.4

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 8080Chloroplast Potential
Chain81 – 403323Malate dehydrogenase, chloroplastic
PRO_0000224149

Regions

Nucleotide binding89 – 957NAD By similarity
Nucleotide binding198 – 2003NAD By similarity

Sites

Active site2581Proton acceptor By similarity
Binding site1151NAD By similarity
Binding site1621Substrate By similarity
Binding site1681Substrate By similarity
Binding site1751NAD By similarity
Binding site2001Substrate By similarity
Binding site2341Substrate By similarity
Binding site3091NAD By similarity

Experimental info

Sequence conflict571A → T in CAA74320. Ref.1
Sequence conflict661K → N in CAA74320. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q9SN86-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 65B54DE66392D229

FASTA40342,406
        10         20         30         40         50         60 
MATATSASLF STVSSSYSKA SSIPHSRLQS VKFNSVPSFT GLKSTSLISG SDSSSLAKTL 

        70         80         90        100        110        120 
RGSVTKAQTS DKKPYGFKIN ASYKVAVLGA AGGIGQPLSL LIKMSPLVST LHLYDIANVK 

       130        140        150        160        170        180 
GVAADLSHCN TPSQVRDFTG PSELADCLKD VNVVVIPAGV PRKPGMTRDD LFNINANIVK 

       190        200        210        220        230        240 
TLVEAVAENC PNAFIHIISN PVNSTVPIAA EVLKKKGVYD PKKLFGVTTL DVVRANTFVS 

       250        260        270        280        290        300 
QKKNLKLIDV DVPVIGGHAG ITILPLLSKT KPSVNFTDEE IQELTVRIQN AGTEVVDAKA 

       310        320        330        340        350        360 
GAGSATLSMA YAAARFVESS LRALDGDGDV YECSFVESTL TDLPFFASRV KIGKNGLEAV 

       370        380        390        400 
IESDLQGLTE YEQKALEALK VELKASIDKG VAFANKPAAA AAN

« Hide

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References

« Hide 'large scale' references
[1]"A novel, non-redox-regulated NAD-dependent malate dehydrogenase from chloroplasts of Arabidopsis thaliana L."
Berkemeyer M., Scheibe R., Ocheretina O.
J. Biol. Chem. 273:27927-27933(1998) [PubMed: 9774405] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
Strain: cv. Columbia.
Tissue: Leaf.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
Mol. Cell. Proteomics 2:325-345(2003) [PubMed: 12766230] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.

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Cross-references

Sequence databases

Y13987 mRNA. Translation: CAA74320.1.
AL132955 Genomic DNA. Translation: CAB61978.1.
AY128281 mRNA. Translation: AAM91090.1.
BT000621 mRNA. Translation: AAN18188.1.
IPIIPI00524343.
PIRT45712.
T51862.
RefSeqNP_190336.1.
UniGeneAt.20474

3D structure databases

HSSPHSSP built from PDB template 1MLD based on UniProtKB P00346.
ModBaseSearch...

Proteomic databases

PRIDEQ9SN86.
ProMEXQ9SN86.

Genome annotation databases

GeneID823906.
GenomeReviewsGene locus AT3G47520 in contig BA000014_GR.
KEGGath:AT3G47520.
NMPDRfig|3702.1.peg.15975.

Organism-specific databases

TAIRAt3g47520.

Phylogenomic databases

OMAQ9SN86. VRSEETE.

Enzyme and pathway databases

BRENDA1.1.1.37. 302.

Gene expression databases

GermOnlineAT3G47520. Arabidopsis thaliana.

Family and domain databases

InterProIPR001557. L-lactate/malate_DH.
IPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_NAD-dep_euk_g_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR11540:SF1. MDH_euk_g_bac. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMDHP_ARATH
AccessionPrimary (citable) accession number: Q9SN86
Secondary accession number(s): O81844
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: May 1, 2000
Last modified: June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents