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Protein

Ras-related protein RABF2b

Gene

RABF2B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endosomal protein that may be involved in endocytosis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 248GTP
Nucleotide bindingi65 – 695GTPBy similarity
Nucleotide bindingi123 – 1264GTP
Nucleotide bindingi153 – 1542GTP

GO - Molecular functioni

  • GTP binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein RABF2b1 Publication
Short name:
AtRABF2b1 Publication
Alternative name(s):
Ras-related protein Ara-71 Publication
Ras-related protein Rab5B1 Publication
Short name:
AtRab5B1 Publication
Gene namesi
Name:RABF2B1 Publication
Synonyms:ARA-71 Publication, RAB5B1 Publication
Ordered Locus Names:At4g19640Imported
ORF Names:F24J7.190Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G19640.

Subcellular locationi

GO - Cellular componenti

  • early endosome Source: UniProtKB
  • early endosome membrane Source: UniProtKB-SubCell
  • endocytic vesicle Source: GO_Central
  • endoplasmic reticulum Source: TAIR
  • endosome Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 191V → T: Loss of interaction with VPS9A. 1 Publication
Mutagenesisi24 – 241S → N: Dominant negative (GDP-bound form); no effect on the interaction with VPS9A. 1 Publication
Mutagenesisi36 – 361V → P: No effect on the interaction with VPS9A. 1 Publication
Mutagenesisi42 – 421T → A: No effect on the interaction with VPS9A. 1 Publication
Mutagenesisi44 – 441G → P: No effect on the interaction with VPS9A. 1 Publication
Mutagenesisi46 – 461A → D: Loss of interaction with VPS9A. 1 Publication
Mutagenesisi47 – 471F → A: Loss of interaction with VPS9A. 1 Publication
Mutagenesisi64 – 641W → A: Loss of interaction with VPS9A. 1 Publication
Mutagenesisi67 – 671A → G: Loss of interaction with VPS9A. 1 Publication
Mutagenesisi69 – 691Q → E: Loss of interaction with VPS9A. 2 Publications
Mutagenesisi69 – 691Q → L: Constitutively active (GTP-bound form); loss of targeting to plasma membrane and interaction with VPS9A. 2 Publications
Mutagenesisi74 – 741S → A: Loss of interaction with VPS9A. 1 Publication
Mutagenesisi75 – 751L → A: Loss of interaction with VPS9A. 1 Publication
Mutagenesisi78 – 781M → A: Loss of interaction with VPS9A. 1 Publication
Mutagenesisi79 – 791Y → A: Loss of interaction with VPS9A. 1 Publication
Mutagenesisi123 – 1231N → I: Blocks nucleotide binding; no effect on the interaction with VPS9A. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Ras-related protein RABF2bPRO_0000406605Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi198 – 1981S-geranylgeranyl cysteineBy similarity
Lipidationi199 – 1991S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

PaxDbiQ9SN68.
PRIDEiQ9SN68.

Expressioni

Tissue specificityi

Expressed in roots and actively dividing cells.1 Publication

Inductioni

Activated by VPS9A.1 Publication

Gene expression databases

GenevisibleiQ9SN68. AT.

Interactioni

Subunit structurei

Interacts with VPS9A homodimer (PubMed:18055610, PubMed:20833725). Interacts with TCTP1 (PubMed:20736351).3 Publications

Protein-protein interaction databases

BioGridi12999. 1 interaction.
IntActiQ9SN68. 2 interactions.
STRINGi3702.AT4G19640.1.

Structurei

Secondary structure

1
200
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 168Combined sources
Helixi23 – 3210Combined sources
Turni36 – 383Combined sources
Beta strandi41 – 433Combined sources
Beta strandi46 – 549Combined sources
Beta strandi57 – 659Combined sources
Helixi70 – 767Combined sources
Helixi77 – 804Combined sources
Beta strandi84 – 918Combined sources
Helixi95 – 11117Combined sources
Beta strandi117 – 1237Combined sources
Turni128 – 1303Combined sources
Helixi135 – 14410Combined sources
Beta strandi148 – 1514Combined sources
Beta strandi154 – 1563Combined sources
Helixi160 – 16910Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EFCX-ray2.09B/D1-179[»]
2EFDX-ray3.00B/D1-179[»]
2EFEX-ray2.08B/D1-179[»]
2EFHX-ray2.10B/D1-179[»]
4G01X-ray2.20B1-179[»]
ProteinModelPortaliQ9SN68.
SMRiQ9SN68. Positions 8-172.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9SN68.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 479Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0092. Eukaryota.
ENOG410YCCP. LUCA.
HOGENOMiHOG000233968.
InParanoidiQ9SN68.
OMAiPTSRTCC.
OrthoDBiEOG09360MTI.
PhylomeDBiQ9SN68.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SN68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAGNKSIN AKLVLLGDVG AGKSSLVLRF VKDQFVEFQE STIGAAFFSQ
60 70 80 90 100
TLAVNDATVK FEIWDTAGQE RYHSLAPMYY RGAAAAIIVF DVTNQASFER
110 120 130 140 150
AKKWVQELQA QGNPNMVMAL AGNKSDLLDA RKVTAEDAQT YAQENGLFFM
160 170 180 190 200
ETSAKTATNV KEIFYEIARR LPRVQPTENP TGMVLPDRAM DRAVSSSCCA
Length:200
Mass (Da):21,873
Last modified:May 1, 2000 - v1
Checksum:i769F75CFC708C6D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038491 mRNA. Translation: BAB32669.1.
AL021768 Genomic DNA. Translation: CAA16940.1.
AL161551 Genomic DNA. Translation: CAB78966.1.
CP002687 Genomic DNA. Translation: AEE84207.1.
AF370309 mRNA. Translation: AAK44124.1.
AY052670 mRNA. Translation: AAK96574.1.
AY063095 mRNA. Translation: AAL34269.1.
PIRiT06157.
RefSeqiNP_193699.1. NM_118084.4.
UniGeneiAt.2030.

Genome annotation databases

EnsemblPlantsiAT4G19640.1; AT4G19640.1; AT4G19640.
GeneIDi827706.
GrameneiAT4G19640.1; AT4G19640.1; AT4G19640.
KEGGiath:AT4G19640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038491 mRNA. Translation: BAB32669.1.
AL021768 Genomic DNA. Translation: CAA16940.1.
AL161551 Genomic DNA. Translation: CAB78966.1.
CP002687 Genomic DNA. Translation: AEE84207.1.
AF370309 mRNA. Translation: AAK44124.1.
AY052670 mRNA. Translation: AAK96574.1.
AY063095 mRNA. Translation: AAL34269.1.
PIRiT06157.
RefSeqiNP_193699.1. NM_118084.4.
UniGeneiAt.2030.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EFCX-ray2.09B/D1-179[»]
2EFDX-ray3.00B/D1-179[»]
2EFEX-ray2.08B/D1-179[»]
2EFHX-ray2.10B/D1-179[»]
4G01X-ray2.20B1-179[»]
ProteinModelPortaliQ9SN68.
SMRiQ9SN68. Positions 8-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12999. 1 interaction.
IntActiQ9SN68. 2 interactions.
STRINGi3702.AT4G19640.1.

Proteomic databases

PaxDbiQ9SN68.
PRIDEiQ9SN68.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G19640.1; AT4G19640.1; AT4G19640.
GeneIDi827706.
GrameneiAT4G19640.1; AT4G19640.1; AT4G19640.
KEGGiath:AT4G19640.

Organism-specific databases

TAIRiAT4G19640.

Phylogenomic databases

eggNOGiKOG0092. Eukaryota.
ENOG410YCCP. LUCA.
HOGENOMiHOG000233968.
InParanoidiQ9SN68.
OMAiPTSRTCC.
OrthoDBiEOG09360MTI.
PhylomeDBiQ9SN68.

Miscellaneous databases

EvolutionaryTraceiQ9SN68.
PROiQ9SN68.

Gene expression databases

GenevisibleiQ9SN68. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAF2B_ARATH
AccessioniPrimary (citable) accession number: Q9SN68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.