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Protein

Ras-related protein RABA4b

Gene

RABA4B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of membrane trafficking. May be required for secretion of cell wall components in cells.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTPBy similarity
Nucleotide bindingi72 – 765GTPBy similarity
Nucleotide bindingi130 – 1334GTPBy similarity
Nucleotide bindingi160 – 1612GTPBy similarity

GO - Molecular functioni

  1. GDP binding Source: GO_Central
  2. GTPase activity Source: GO_Central
  3. GTP binding Source: GO_Central
  4. kinase binding Source: UniProtKB

GO - Biological processi

  1. endocytic recycling Source: TAIR
  2. GTP catabolic process Source: GO_Central
  3. intracellular protein transport Source: GO_Central
  4. Rab protein signal transduction Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_259156. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein RABA4b
Short name:
AtRABA4b
Alternative name(s):
Ras-related protein GB3
Short name:
AtGB3
Ras-related protein Rab11G
Short name:
AtRab11G
Gene namesi
Name:RABA4B
Synonyms:GB3, RAB11G
Ordered Locus Names:At4g39990
ORF Names:T5J17.160
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G39990.

Subcellular locationi

Early endosome membrane 1 Publication. Golgi apparatustrans-Golgi network membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  1. early endosome membrane Source: UniProtKB
  2. plasma membrane Source: TAIR
  3. trans-Golgi network membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 224223Ras-related protein RABA4bPRO_0000407345Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Lipidationi220 – 2201S-geranylgeranyl cysteineBy similarity
Lipidationi221 – 2211S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Proteomic databases

PaxDbiQ9SMQ6.
PRIDEiQ9SMQ6.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves and flowers. Expressed in tips of growing root hair cells.1 Publication

Gene expression databases

GenevestigatoriQ9SMQ6.

Interactioni

Protein-protein interaction databases

BioGridi15440. 1 interaction.
IntActiQ9SMQ6. 1 interaction.
STRINGi3702.AT4G39990.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SMQ6.
SMRiQ9SMQ6. Positions 15-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 549Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
InParanoidiQ9SMQ6.
OMAiFEHIPRW.
PhylomeDBiQ9SMQ6.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SMQ6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGGGGYGGA SGKVDYVFKV VLIGDSAVGK SQLLARFARD EFSMDSKATI
60 70 80 90 100
GVEFQTRTLS IEQKSIKAQI WDTAGQERYR AVTSAYYRGA VGAMLVYDMT
110 120 130 140 150
KRETFEHIPR WLEELRAHAD KNIVIILIGN KSDLEDQRAV PTEDAKEFAE
160 170 180 190 200
KEGLFFLETS ALNATNVENS FNTLMTQIYN TVNKKNLASE GDSNNPGSLA
210 220
GKKILIPGSG QEIPAKTSTC CTSS
Length:224
Mass (Da):24,407
Last modified:May 1, 2000 - v1
Checksum:iFA2C5E5A0DC2025B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti191 – 1911G → C in AAA87884. (PubMed:8843944)Curated
Sequence conflicti208 – 2081G → A in AAM66946. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46926 mRNA. Translation: AAA87884.1.
AL035708 Genomic DNA. Translation: CAB38912.1.
AL161596 Genomic DNA. Translation: CAB80662.1.
CP002687 Genomic DNA. Translation: AEE87150.1.
AY072448 mRNA. Translation: AAL62440.1.
AY128914 mRNA. Translation: AAM91314.1.
AY088624 mRNA. Translation: AAM66946.1.
PIRiT06105.
RefSeqiNP_195709.1. NM_120163.4.
UniGeneiAt.1649.
At.48966.

Genome annotation databases

EnsemblPlantsiAT4G39990.1; AT4G39990.1; AT4G39990.
GeneIDi830160.
KEGGiath:AT4G39990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46926 mRNA. Translation: AAA87884.1.
AL035708 Genomic DNA. Translation: CAB38912.1.
AL161596 Genomic DNA. Translation: CAB80662.1.
CP002687 Genomic DNA. Translation: AEE87150.1.
AY072448 mRNA. Translation: AAL62440.1.
AY128914 mRNA. Translation: AAM91314.1.
AY088624 mRNA. Translation: AAM66946.1.
PIRiT06105.
RefSeqiNP_195709.1. NM_120163.4.
UniGeneiAt.1649.
At.48966.

3D structure databases

ProteinModelPortaliQ9SMQ6.
SMRiQ9SMQ6. Positions 15-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15440. 1 interaction.
IntActiQ9SMQ6. 1 interaction.
STRINGi3702.AT4G39990.1-P.

Proteomic databases

PaxDbiQ9SMQ6.
PRIDEiQ9SMQ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G39990.1; AT4G39990.1; AT4G39990.
GeneIDi830160.
KEGGiath:AT4G39990.

Organism-specific databases

TAIRiAT4G39990.

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
InParanoidiQ9SMQ6.
OMAiFEHIPRW.
PhylomeDBiQ9SMQ6.

Enzyme and pathway databases

ReactomeiREACT_259156. Translocation of GLUT4 to the plasma membrane.

Gene expression databases

GenevestigatoriQ9SMQ6.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and isoprenylation of plant GTP-binding proteins."
    Biermann B.J., Randall S.K., Crowell D.N.
    Plant Mol. Biol. 31:1021-1028(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Analysis of the small GTPase gene superfamily of Arabidopsis."
    Vernoud V., Horton A.C., Yang Z., Nielsen E.
    Plant Physiol. 131:1191-1208(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "The Arabidopsis Rab GTPase RabA4b localizes to the tips of growing root hair cells."
    Preuss M.L., Serna J., Falbel T.G., Bednarek S.Y., Nielsen E.
    Plant Cell 16:1589-1603(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Electron tomography of RabA4b- and PI-4K?1-labeled trans Golgi network compartments in Arabidopsis."
    Kang B.H., Nielsen E., Preuss M.L., Mastronarde D., Staehelin L.A.
    Traffic 12:313-329(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAA4B_ARATH
AccessioniPrimary (citable) accession number: Q9SMQ6
Secondary accession number(s): Q38923, Q8L958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 1, 2000
Last modified: February 4, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.