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Reviewed, UniProtKB/Swiss-Prot Q9SML8 (MDHC_BETVU)

Last modified June 16, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Malate dehydrogenase, cytoplasmic
    EC=1.1.1.37
Gene names
Name: NR1
OrganismBeta vulgaris (Sugar beet)
Taxonomic identifier161934 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Beta

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Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

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Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: EC

binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332Malate dehydrogenase, cytoplasmic
PRO_0000113414

Regions

Nucleotide binding12 – 187NAD By similarity
Nucleotide binding130 – 1323NAD By similarity

Sites

Active site1881Proton acceptor By similarity
Binding site931Substrate By similarity
Binding site991Substrate By similarity
Binding site1061NAD By similarity
Binding site1131NAD By similarity
Binding site1321Substrate By similarity
Binding site1631Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9SML8-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 2B9E6495EF0CD12A

FASTA33235,434
        10         20         30         40         50         60 
MAVEPLRVLV TGAAGQIGYA LVPMIARGVM LGANQPVILH MLDIPPAAEA LNGVKMELVD 

        70         80         90        100        110        120 
AAFPLLKGVV ATTDVAEACK GVNVAVMVGG FPRKEGMERK DVMPKNVSIY KSQASALEQY 

       130        140        150        160        170        180 
AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNITCLTRLD HNRALGQISE RLNAQVSDVK 

       190        200        210        220        230        240 
NVIIWGNHSS SQYPDVNPCT VKTGSGEKAV RELVADDAWL NGEFITTVQQ RGAAIIKARK 

       250        260        270        280        290        300 
LSSALSAASS ACDHIRDWVL GTPEGTWVSM GVYSDGSYNV PAGIIYSFPV TCKDGEWKIV 

       310        320        330 
QGLPIDEVSR QKMDATGAEL VEEKALAYSC LT

« Hide

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References

[1]"A Beta vulgaris cDNA coding for a putative cytoplasmatic malate dehydrogenase."
Juergensen K., Buck F., Lange S., Kleine M., Grundler F.M.W.
Plant Gene Register PGR00-023
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

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Cross-references

Sequence databases

AJ251083 mRNA. Translation: CAB61618.1.

3D structure databases

HSSPHSSP built from PDB template 4MDH based on UniProtKB P11708.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.1.1.37. 124.

Family and domain databases

InterProIPR001557. L-lactate/malate_DH.
IPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_SF1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR23382. MDH_SF1. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
ProDomPD003052. Mal_dehydrog. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMDHC_BETVU
AccessionPrimary (citable) accession number: Q9SML8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents