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Q9SMH3

- DYH1A_CHLRE

UniProt

Q9SMH3 - DYH1A_CHLRE

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Protein
Dynein-1-alpha heavy chain, flagellar inner arm I1 complex
Gene
DHC1, IDA1, PF9
Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Force generating protein of eukaryotic cilia and flagella. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for assembly of the I1 inner arm complex and its targeting to the appropriate axoneme location. Also required for phototaxis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi960 – 9678ATP Reviewed prediction
Nucleotide bindingi1958 – 19658ATP Reviewed prediction
Nucleotide bindingi2242 – 22498ATP Reviewed prediction
Nucleotide bindingi2588 – 25958ATP Reviewed prediction
Nucleotide bindingi2945 – 29528ATP Reviewed prediction
Nucleotide bindingi3680 – 36878ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATPase activity Source: InterPro
  3. microtubule motor activity Source: InterPro

GO - Biological processi

  1. cell projection organization Source: UniProtKB-KW
  2. microtubule-based movement Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein-1-alpha heavy chain, flagellar inner arm I1 complex
Alternative name(s):
1-alpha DHC
Dynein-1, subspecies f
Gene namesi
Name:DHC1
Synonyms:IDA1, PF9
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Subcellular locationi

GO - Cellular componenti

  1. cilium Source: UniProtKB-KW
  2. cytoplasm Source: UniProtKB-KW
  3. dynein complex Source: UniProtKB-KW
  4. microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Dynein, Flagellum, Microtubule

Pathology & Biotechi

Disruption phenotypei

Cells swim slowly with near normal beat frequencies but aberrant waveforms, and are unable to phototax.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 46254625Dynein-1-alpha heavy chain, flagellar inner arm I1 complex
PRO_0000114646Add
BLAST

Proteomic databases

PRIDEiQ9SMH3.

Expressioni

Inductioni

By deflagellation.

Interactioni

Subunit structurei

The I1 inner arm complex (also known as the f dynein complex) is a two-headed isoform composed of two heavy chains (1-alpha and 1-beta), three intermediate chains and three light chains. I1 occupies a specific position proximal to the first radial spoke and repeats every 96 nm along the length of the axoneme.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9SMH3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 19191919Stem By similarity
Add
BLAST
Regioni1920 – 2141222AAA 1 By similarity
Add
BLAST
Regioni2201 – 2437237AAA 2 By similarity
Add
BLAST
Regioni2550 – 2800251AAA 3 By similarity
Add
BLAST
Regioni2906 – 3155250AAA 4 By similarity
Add
BLAST
Regioni3192 – 3494303Stalk By similarity
Add
BLAST
Regioni3542 – 3773232AAA 5 By similarity
Add
BLAST
Regioni3998 – 4216219AAA 6 By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1227 – 125933 Reviewed prediction
Add
BLAST
Coiled coili1339 – 140971 Reviewed prediction
Add
BLAST
Coiled coili3192 – 3297106 Reviewed prediction
Add
BLAST
Coiled coili3400 – 349495 Reviewed prediction
Add
BLAST
Coiled coili3701 – 378888 Reviewed prediction
Add
BLAST

Domaini

Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
A construct expressing the first 1249 amino acids but lacking the motor domain is able to assemble I1 complexes and target them to their correct location on the axoneme, although motility is not normal. Phototaxis is also restored.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG5245.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011704. ATPase_dyneun-rel_AAA.
IPR026983. DHC_fam.
IPR024743. Dynein_HC_stalk.
IPR024317. Dynein_heavy_chain_D4_dom.
IPR004273. Dynein_heavy_dom.
IPR013594. Dynein_heavy_dom-1.
IPR013602. Dynein_heavy_dom-2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10676. PTHR10676. 1 hit.
PfamiPF07728. AAA_5. 1 hit.
PF12780. AAA_8. 1 hit.
PF08385. DHC_N1. 1 hit.
PF08393. DHC_N2. 1 hit.
PF03028. Dynein_heavy. 1 hit.
PF12777. MT. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 4 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 6 hits.

Sequencei

Sequence statusi: Complete.

Q9SMH3-1 [UniParc]FASTAAdd to Basket

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MDRRLEWVKE KACLGLGVEP NLFEAAIANP ESRARVTAFL DGTVTSSALL     50
FALEEATIYV EEYQEVLAEE QAPEAEDGEG EEHDGQEPGE AGGEGAEGST 100
APGDSGDGQP EDAPAAAAEA NGANPEDEAA APADGAADGA AGEGGEEGDG 150
AEGDEPPAPP APKYVRRVIS VPKVVSKLNV ALGSLPEELS VFPVFYFILN 200
RSGHVAAEEL DSAVEFGLLS EGPSLRILEQ MLSSVFVPIL VQMSGGDVAS 250
GGVLMQSMTD NSHRELLGNM QKFHSQVTQA LQQLTGDVTL QLPDFPLEDM 300
DRAAADTDLV MQLEQYMAEW SQVLASVLQR ESQKHPTGKG PLAEIEFWRE 350
RNAVLSSLYE QLNLPQVKRM ILVVEKGSDD RNLMAGFKSQ LGELTKLATE 400
ARDNVKFLTT LERHFKNIAT GPLGGILDTL PPMMNALRMV WIISRHYSDD 450
QRMGSLFQRI AREIGDRVEA AVDLRHIFRM TSADAVELLK VCKSVLEHWL 500
QTYMAMREKI ELSGRDARWE FPKQLLFART NYMAEICTDL IEMVEIVDDF 550
FRFLGPELKT VTGDTAGIDR VVHRVVAMYE PIESISFNVF DYGNQHEWKA 600
AKQQFYADNE DIKEATRELI DTSFRKLRSA EGACELLQSF KSIKSKGAIQ 650
KQVMNKFNDI LEQFAREIEQ TADIFERNKD APPVTKNQPP VAGAIKWVRS 700
LLERLKRTMA KLLSTEEEII RTTELGQAVE SKFKSFARSV MLTEKKWFSS 750
WSDSINGVAM QHLKQTIFRR SAATNRVEVN FHPDLVRLIR ETRYLDRMGF 800
PIPEIALNVA LQEDKFLQWL EGLNSMLFKY YESIDQLTPV ERELMERKLE 850
ELESCLQPGF TILNWNSLGI TEFIGTCDKA IATFQQLVKQ VQKNSGIIEQ 900
VVYAIAGAQL VTEPEEGAEV MDLQEFYEDI ERQRLAALES LVKKYRTISP 950
LLGKIEEVVA GTNSGKSPAL SSYYSFWERA IFNALNTMVL CAMTKLQDMI 1000
EQRSKHAEGG RKPPLFKVTV SLQSVDVVVQ PPMTEVNKAL GRLVRSLVES 1050
TKAFVRWMDG TCVETPEQRG ATDDDEPIVF TFYWDVAANP QVIKTMLNLN 1100
QSIQRAITSV NKYAESWRRH QALWKTDKNS VLDKFKARDP SAAQFEDKLS 1150
KYAKMATEIS AQAKDFDQDF IRVSCHALAS SVCDEAQAWV RAIAQTMREL 1200
DAVTESQLRD KIAKYQTALH RPPDTLEELK QVLNTVNTIR GESMVMELRY 1250
ADLEERYRTR LLYATNPEEE SQCAHELASA SQVRALWTEL LNEAEAVDWS 1300
LEETKKKFSE TTRSQVSDFA AITAELWEKF RTTGPGLPTV ELASGLDELH 1350
KYESNLADAL RQREQLVLAE KLFGMEITAY PELAQLESEI RKLAQVYGVY 1400
AEHAEAVRQY GGQLWSELDV GKMMAGTEAI LTKLRKLKSL KLLPVYELVE 1450
KEIQGFYNSL PLMKELKSEA LRKRHWTRLM EVTGQEFDMD PKTFTLGNMF 1500
AMQLHKYAEE IGKITNAAVK ELTIESEIRK LADVWREQRF ELGKYMKGPE 1550
DRGWVLRSTE DILVLLEDMG LNLQSMMASP FVRPFLTEVR AWEQKLSLIG 1600
ECIEVWMHVQ RKWMYLESIF VGSDDIRHQL PAEAKRFDNI DRQWQKIMND 1650
TAKNTVVLDA CMADGRLDLL KSLSEQLEVC QKSLSEYLDT KRCAFPRFYF 1700
ISDDELLSIL GTSDPTSVQE HMLKLFDNCA ALVFGRGNKT ITGMVSSEKE 1750
GFEFRNVVPI EGAVELWMTN VEAEMRKTLY QITKEGIFFY AKTPRTKWIS 1800
ENLGMVTLVG SQIWWTWETE DVFRRVRDGN KHSMKEFAAK LTGQLSELTS 1850
MVRSDLSNEV RKKVNTLIII DVHARDIIDT YVRDSIVDAR EFAWESQLRF 1900
YWDRQQDDIL IRQCTGLFKY GYEYMGLNGR LVITALTDRC YMTLTTALTY 1950
RLGGAPAGPA GTGKTETTKD LAKSMALLCV VFNCGEGLDY KAMGSIFSGL 2000
VQCGAWGCFD EFNRIEAEVL SVVSSQIKNI QEALKNDLTR FQFEGKEISI 2050
DPRTGIFITM NPGYAGRTEL PDNLKALFRP VTMVVPDLEQ ICEIMLFSEG 2100
FDSAKVLAKK MTVLYKLSRE QLSKQHHYDF GLRALKSVLV MAGSLKRGAP 2150
DMSEQLVLMR ALRDMNLPKF IFDDVPLFLG LINDLFPGMD CPRVRYPQFN 2200
DVVEADLADQ GFKVLTEPSE QVDKVVQLYE VMMTRHTTMV VGQTGGGKTV 2250
ILNTLARAQT KLGKKTHLYT INPKAISVAE LYGVLDKDTR DWTDGLLSNI 2300
FREMNKPLPA ERDEARYLVF DGDVDAVWVE NMNSVMDDNK LLTLPNGERI 2350
RLQNHCKLLF EVFDLQYASP ATISRCGMVY VDSRNLGYKP YIYTWLNSRA 2400
KQAEVDILRG LFEKYAVPSV DWILEGIDGE ELVRRPKQAV PVTNLNMITQ 2450
LCNLLNATIT DHPRMSDPQI LEAIFIFCTI WSLGAAIVQR PESPDRDRFD 2500
AFVKHIASMG LVDGERVAAT QLPARSLYEY CFDTNEGVWK SWRSYLQPYE 2550
PPADGAFAKI LVPTVDVVRS TWLLNTVVAA GKPCLFVGES GTAKSVTIAN 2600
YLAHLDSTIN IVLNVNFSSR TSSLDVQRAI EDSTEKRTKD TYGPPMGKRL 2650
LMFIDDLNMP RVDTYGTQQP IALLKLFIER KGLYDRGKEL SWKNMKDVQV 2700
VGAMGPPGGA RNPVDPRFIS LFSVFEIQFP SNENLRTIYQ AILSRHLAKL 2750
PTDEIRDQLG ERLTDVTLEL YNFIIDKLPP TPSRFHYIFN LRDLSRIYEG 2800
LLLTVGDVFK TPEQFLRLWR NECLRVLHDR LISTDDKRVM TERLEALVQQ 2850
KFPNLAAHTL ASPVLFGDFK NVINELQGEG EVAPRMYDDL GDYNSIKPLF 2900
EDVMTNFYNR KRKPMNLVFF EDALEHLTRI HRTLRLPQGN CLLVGVGGSG 2950
KQSLSKLAAF TAGCEVFEIT LTRGYDELAF REDLKRLYAM LGSDNKRVMF 3000
LFTDAHVADE GFLELINNML TSGMVPALYD GAEKDGLIGS VRAEVEKKGL 3050
LATKESCWSY YVDKCRNNLH VVLAMSPVGE TLRSRCRNFP GMVNNTVIDW 3100
FEPWPEQALT SVASVFLAEE ALPEALRPQI VEHMVTVHQS VRTFSTRFLE 3150
ELRRYNYVTP KNYLDFINNY KRALATNRRT IEDTVTRLSG GLEKLIQAAV 3200
EVDAMQKELS QAQVVVAQAT KECNELLEVI STNTVDVETK AKAAAIKEAQ 3250
LKVDSEQIAI EKAEAEAALE EAIPALEEAA AALQDLSKDH ITEIRSYAKP 3300
PEQVQKVCEC VVILRNIKDV SWLGAKSMMA DGNFLRSLVE FDKDSLTDKQ 3350
VKKVKEYFKD PKAPLTYDSL RAISTAGAGL LKWVLAMVNY NNVARTVEPK 3400
RKKVAESEKN LRIAQKDLAS TKLELQSLND QLGKLRTQFE EKTAEQQDLK 3450
AKADLMERRL IAASKLIAGL GSERERWTRD IADLESRRDR LIGDCLLTSS 3500
FLSYTGAFTA TYRHAMVYEM WQDDVKARGV PVTQPFRLEA LLTSDVETTG 3550
WASEGLPSDE LSIQNGILTV RANRWPLCID PQMQAVNWIK SREGKMLEGK 3600
VKTFNDSDFL KQLELSIQYG FPFLFENLDE YIDPVIDPVL EKNLVPGDGK 3650
FVIKLGDKEV EWDSNFRLYM TSKLSNPHYG PEISGKTMII NYGVTQQGLT 3700
EQLLNVTLRH ERSDLEEARE ALIKQMSENK ATLQALEDTL LRELSNAQGN 3750
ILDNSELIAT LESAKLKAVE IAEKLEASKV TAAEIEETRV RYSPAAKRGA 3800
ILFFVIAGLS AITNMYEYSL ASFLVVFNGS LHSSRRDASI EGRLRNIIDT 3850
LTYDVYAYTC LGLFERHKLM FSFQMTCKIL EGDTPLDPQL LDFFLKGNLS 3900
LEKAARRKPF DWFPDAGWQD LMRLVELGQK KIGADGRMHA LGSLANDVES 3950
DEAAWRTWYD LEAPEEAELP CGYQSFLSDF EKLCLMRCLR MDRVTVGITR 4000
FVIGVMGEKY VQPPVLEYRS IYKQSTETTP IVFVLSPGAD PAFDVFKLGE 4050
EMGFRPGAKL KYMALGQGMG PKAQELIETG ATRGLWIMLQ NCHLLPTWLK 4100
TLEKILEKIT KPHADFRLWL TTELTDRFPL GVLQRSLKVV TEPPNGLKLN 4150
MRQSYSKITE EVLADCPHQA FRPLVYVLGF FHAVVQERRK YGKLGWNVPY 4200
DFNETDFRIS MALISTYLTK AWDAQDDLIP WGTLRYLIGE AMYGGRVSDS 4250
YDRRILTTYL DEYLGDFLFD TFQPFRFYAC KDYEIAIPQT GSRDTYLKAV 4300
EALPLVQSPE AFGLNANADI SYYTSATKAI WTDLVDLQPR TGGGGGGVAR 4350
EEFIGGVARD IAAKIPEPFD LPQLRKELGT PSPTQVVLLQ ELERWNSVLG 4400
VMVSSLRDLQ RALSGEIGFS SRLEELASSL YNGKLPAMWA RLNPATEKAL 4450
GAWMLWFGRR YRQYKDWTEH GEPKVIWLSG LHIPETYIAA LVQAACRDKG 4500
WPLDKSTLYT KVTKFTDPYQ VSERPKYGCY MSGLYLEGAA WDLEASQLRK 4550
QDPKVLVNEL PILQVIPIEA NKLKLANTFR APVYVTQARR NAMGVGLVFD 4600
ADLASAEHSS HWVLQGVALV LNIDQ 4625
Length:4,625
Mass (Da):522,843
Last modified:May 1, 2000 - v1
Checksum:i875F51048AF99340
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ243806 Genomic DNA. Translation: CAB56598.1.
U61364 Genomic DNA. Translation: AAC49514.1.
PIRiS72239.

Cross-referencesi

Web resourcesi

Protein Spotlight

The kink behind the wriggle - Issue 34 of May 2003

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ243806 Genomic DNA. Translation: CAB56598.1 .
U61364 Genomic DNA. Translation: AAC49514.1 .
PIRi S72239.

3D structure databases

ProteinModelPortali Q9SMH3.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9SMH3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG5245.

Family and domain databases

Gene3Di 3.40.50.300. 4 hits.
InterProi IPR003593. AAA+_ATPase.
IPR011704. ATPase_dyneun-rel_AAA.
IPR026983. DHC_fam.
IPR024743. Dynein_HC_stalk.
IPR024317. Dynein_heavy_chain_D4_dom.
IPR004273. Dynein_heavy_dom.
IPR013594. Dynein_heavy_dom-1.
IPR013602. Dynein_heavy_dom-2.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10676. PTHR10676. 1 hit.
Pfami PF07728. AAA_5. 1 hit.
PF12780. AAA_8. 1 hit.
PF08385. DHC_N1. 1 hit.
PF08393. DHC_N2. 1 hit.
PF03028. Dynein_heavy. 1 hit.
PF12777. MT. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 4 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 6 hits.
ProtoNeti Search...

Publicationsi

  1. "Domains in the 1-alpha dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas."
    Myster S.H., Knott J.A., Wysocki K.M., O'Toole E.T., Porter M.E.
    J. Cell Biol. 146:801-818(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, MUTAGENESIS.
    Strain: 21gr / CC-1690.
  2. "The dynein gene family in Chlamydomonas reinhardtii."
    Porter M.E., Knott J.A., Myster S.H., Farlow S.J.
    Genetics 144:569-585(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1914-2064.
    Strain: 137c / CC-125.
  3. "High-pressure liquid chromatography fractionation of Chlamydomonas dynein extracts and characterization of inner-arm dynein subunits."
    Goodenough U.W., Gebhart B., Mermall V., Mitchell D.R., Heuser J.E.
    J. Mol. Biol. 194:481-494(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: DYNEIN COMPLEX ELECTRON MICROSCOPY.
    Strain: CC-620.
  4. "Three distinct inner dynein arms in Chlamydomonas flagella: molecular composition and location in the axoneme."
    Piperno G., Ramanis Z., Smith E.F., Sale W.S.
    J. Cell Biol. 110:379-389(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  5. "Phosphoregulation of an inner dynein arm complex in Chlamydomonas reinhardtii is altered in phototactic mutant strains."
    King S.J., Dutcher S.K.
    J. Cell Biol. 136:177-191(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: REQUIREMENT OF I1 DYNEIN COMPLEX FOR PHOTOTAXIS.
  6. "The Chlamydomonas Dhc1 gene encodes a dynein heavy chain subunit required for assembly of the I1 inner arm complex."
    Myster S.H., Knott J.A., O'Toole E.T., Porter M.E.
    Mol. Biol. Cell 8:607-620(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOLATION, DISRUPTION PHENOTYPE.
    Strain: 21gr / CC-1690.

Entry informationi

Entry nameiDYH1A_CHLRE
AccessioniPrimary (citable) accession number: Q9SMH3
Secondary accession number(s): Q96388
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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