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Q9SMH3

- DYH1A_CHLRE

UniProt

Q9SMH3 - DYH1A_CHLRE

Protein

Dynein-1-alpha heavy chain, flagellar inner arm I1 complex

Gene

DHC1

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Force generating protein of eukaryotic cilia and flagella. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for assembly of the I1 inner arm complex and its targeting to the appropriate axoneme location. Also required for phototaxis.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi960 – 9678ATPSequence Analysis
    Nucleotide bindingi1958 – 19658ATPSequence Analysis
    Nucleotide bindingi2242 – 22498ATPSequence Analysis
    Nucleotide bindingi2588 – 25958ATPSequence Analysis
    Nucleotide bindingi2945 – 29528ATPSequence Analysis
    Nucleotide bindingi3680 – 36878ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. microtubule motor activity Source: InterPro

    GO - Biological processi

    1. cell projection organization Source: UniProtKB-KW
    2. microtubule-based movement Source: InterPro

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Cilium biogenesis/degradation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dynein-1-alpha heavy chain, flagellar inner arm I1 complex
    Alternative name(s):
    1-alpha DHC
    Dynein-1, subspecies f
    Gene namesi
    Name:DHC1
    Synonyms:IDA1, PF9
    OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
    Taxonomic identifieri3055 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

    Subcellular locationi

    GO - Cellular componenti

    1. cilium Source: UniProtKB-KW
    2. cytoplasm Source: UniProtKB-KW
    3. dynein complex Source: UniProtKB-KW
    4. microtubule Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell projection, Cilium, Cytoplasm, Cytoskeleton, Dynein, Flagellum, Microtubule

    Pathology & Biotechi

    Disruption phenotypei

    Cells swim slowly with near normal beat frequencies but aberrant waveforms, and are unable to phototax.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 46254625Dynein-1-alpha heavy chain, flagellar inner arm I1 complexPRO_0000114646Add
    BLAST

    Proteomic databases

    PRIDEiQ9SMH3.

    Expressioni

    Inductioni

    By deflagellation.

    Interactioni

    Subunit structurei

    The I1 inner arm complex (also known as the f dynein complex) is a two-headed isoform composed of two heavy chains (1-alpha and 1-beta), three intermediate chains and three light chains. I1 occupies a specific position proximal to the first radial spoke and repeats every 96 nm along the length of the axoneme.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SMH3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 19191919StemBy similarityAdd
    BLAST
    Regioni1920 – 2141222AAA 1By similarityAdd
    BLAST
    Regioni2201 – 2437237AAA 2By similarityAdd
    BLAST
    Regioni2550 – 2800251AAA 3By similarityAdd
    BLAST
    Regioni2906 – 3155250AAA 4By similarityAdd
    BLAST
    Regioni3192 – 3494303StalkBy similarityAdd
    BLAST
    Regioni3542 – 3773232AAA 5By similarityAdd
    BLAST
    Regioni3998 – 4216219AAA 6By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1227 – 125933Sequence AnalysisAdd
    BLAST
    Coiled coili1339 – 140971Sequence AnalysisAdd
    BLAST
    Coiled coili3192 – 3297106Sequence AnalysisAdd
    BLAST
    Coiled coili3400 – 349495Sequence AnalysisAdd
    BLAST
    Coiled coili3701 – 378888Sequence AnalysisAdd
    BLAST

    Domaini

    Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
    A construct expressing the first 1249 amino acids but lacking the motor domain is able to assemble I1 complexes and target them to their correct location on the axoneme, although motility is not normal. Phototaxis is also restored.

    Sequence similaritiesi

    Belongs to the dynein heavy chain family.Curated

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiCOG5245.

    Family and domain databases

    Gene3Di3.40.50.300. 4 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR011704. ATPase_dyneun-rel_AAA.
    IPR026983. DHC_fam.
    IPR024743. Dynein_HC_stalk.
    IPR024317. Dynein_heavy_chain_D4_dom.
    IPR004273. Dynein_heavy_dom.
    IPR013594. Dynein_heavy_dom-1.
    IPR013602. Dynein_heavy_dom-2.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10676. PTHR10676. 1 hit.
    PfamiPF07728. AAA_5. 1 hit.
    PF12780. AAA_8. 1 hit.
    PF08385. DHC_N1. 1 hit.
    PF08393. DHC_N2. 1 hit.
    PF03028. Dynein_heavy. 1 hit.
    PF12777. MT. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 4 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 6 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9SMH3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRRLEWVKE KACLGLGVEP NLFEAAIANP ESRARVTAFL DGTVTSSALL     50
    FALEEATIYV EEYQEVLAEE QAPEAEDGEG EEHDGQEPGE AGGEGAEGST 100
    APGDSGDGQP EDAPAAAAEA NGANPEDEAA APADGAADGA AGEGGEEGDG 150
    AEGDEPPAPP APKYVRRVIS VPKVVSKLNV ALGSLPEELS VFPVFYFILN 200
    RSGHVAAEEL DSAVEFGLLS EGPSLRILEQ MLSSVFVPIL VQMSGGDVAS 250
    GGVLMQSMTD NSHRELLGNM QKFHSQVTQA LQQLTGDVTL QLPDFPLEDM 300
    DRAAADTDLV MQLEQYMAEW SQVLASVLQR ESQKHPTGKG PLAEIEFWRE 350
    RNAVLSSLYE QLNLPQVKRM ILVVEKGSDD RNLMAGFKSQ LGELTKLATE 400
    ARDNVKFLTT LERHFKNIAT GPLGGILDTL PPMMNALRMV WIISRHYSDD 450
    QRMGSLFQRI AREIGDRVEA AVDLRHIFRM TSADAVELLK VCKSVLEHWL 500
    QTYMAMREKI ELSGRDARWE FPKQLLFART NYMAEICTDL IEMVEIVDDF 550
    FRFLGPELKT VTGDTAGIDR VVHRVVAMYE PIESISFNVF DYGNQHEWKA 600
    AKQQFYADNE DIKEATRELI DTSFRKLRSA EGACELLQSF KSIKSKGAIQ 650
    KQVMNKFNDI LEQFAREIEQ TADIFERNKD APPVTKNQPP VAGAIKWVRS 700
    LLERLKRTMA KLLSTEEEII RTTELGQAVE SKFKSFARSV MLTEKKWFSS 750
    WSDSINGVAM QHLKQTIFRR SAATNRVEVN FHPDLVRLIR ETRYLDRMGF 800
    PIPEIALNVA LQEDKFLQWL EGLNSMLFKY YESIDQLTPV ERELMERKLE 850
    ELESCLQPGF TILNWNSLGI TEFIGTCDKA IATFQQLVKQ VQKNSGIIEQ 900
    VVYAIAGAQL VTEPEEGAEV MDLQEFYEDI ERQRLAALES LVKKYRTISP 950
    LLGKIEEVVA GTNSGKSPAL SSYYSFWERA IFNALNTMVL CAMTKLQDMI 1000
    EQRSKHAEGG RKPPLFKVTV SLQSVDVVVQ PPMTEVNKAL GRLVRSLVES 1050
    TKAFVRWMDG TCVETPEQRG ATDDDEPIVF TFYWDVAANP QVIKTMLNLN 1100
    QSIQRAITSV NKYAESWRRH QALWKTDKNS VLDKFKARDP SAAQFEDKLS 1150
    KYAKMATEIS AQAKDFDQDF IRVSCHALAS SVCDEAQAWV RAIAQTMREL 1200
    DAVTESQLRD KIAKYQTALH RPPDTLEELK QVLNTVNTIR GESMVMELRY 1250
    ADLEERYRTR LLYATNPEEE SQCAHELASA SQVRALWTEL LNEAEAVDWS 1300
    LEETKKKFSE TTRSQVSDFA AITAELWEKF RTTGPGLPTV ELASGLDELH 1350
    KYESNLADAL RQREQLVLAE KLFGMEITAY PELAQLESEI RKLAQVYGVY 1400
    AEHAEAVRQY GGQLWSELDV GKMMAGTEAI LTKLRKLKSL KLLPVYELVE 1450
    KEIQGFYNSL PLMKELKSEA LRKRHWTRLM EVTGQEFDMD PKTFTLGNMF 1500
    AMQLHKYAEE IGKITNAAVK ELTIESEIRK LADVWREQRF ELGKYMKGPE 1550
    DRGWVLRSTE DILVLLEDMG LNLQSMMASP FVRPFLTEVR AWEQKLSLIG 1600
    ECIEVWMHVQ RKWMYLESIF VGSDDIRHQL PAEAKRFDNI DRQWQKIMND 1650
    TAKNTVVLDA CMADGRLDLL KSLSEQLEVC QKSLSEYLDT KRCAFPRFYF 1700
    ISDDELLSIL GTSDPTSVQE HMLKLFDNCA ALVFGRGNKT ITGMVSSEKE 1750
    GFEFRNVVPI EGAVELWMTN VEAEMRKTLY QITKEGIFFY AKTPRTKWIS 1800
    ENLGMVTLVG SQIWWTWETE DVFRRVRDGN KHSMKEFAAK LTGQLSELTS 1850
    MVRSDLSNEV RKKVNTLIII DVHARDIIDT YVRDSIVDAR EFAWESQLRF 1900
    YWDRQQDDIL IRQCTGLFKY GYEYMGLNGR LVITALTDRC YMTLTTALTY 1950
    RLGGAPAGPA GTGKTETTKD LAKSMALLCV VFNCGEGLDY KAMGSIFSGL 2000
    VQCGAWGCFD EFNRIEAEVL SVVSSQIKNI QEALKNDLTR FQFEGKEISI 2050
    DPRTGIFITM NPGYAGRTEL PDNLKALFRP VTMVVPDLEQ ICEIMLFSEG 2100
    FDSAKVLAKK MTVLYKLSRE QLSKQHHYDF GLRALKSVLV MAGSLKRGAP 2150
    DMSEQLVLMR ALRDMNLPKF IFDDVPLFLG LINDLFPGMD CPRVRYPQFN 2200
    DVVEADLADQ GFKVLTEPSE QVDKVVQLYE VMMTRHTTMV VGQTGGGKTV 2250
    ILNTLARAQT KLGKKTHLYT INPKAISVAE LYGVLDKDTR DWTDGLLSNI 2300
    FREMNKPLPA ERDEARYLVF DGDVDAVWVE NMNSVMDDNK LLTLPNGERI 2350
    RLQNHCKLLF EVFDLQYASP ATISRCGMVY VDSRNLGYKP YIYTWLNSRA 2400
    KQAEVDILRG LFEKYAVPSV DWILEGIDGE ELVRRPKQAV PVTNLNMITQ 2450
    LCNLLNATIT DHPRMSDPQI LEAIFIFCTI WSLGAAIVQR PESPDRDRFD 2500
    AFVKHIASMG LVDGERVAAT QLPARSLYEY CFDTNEGVWK SWRSYLQPYE 2550
    PPADGAFAKI LVPTVDVVRS TWLLNTVVAA GKPCLFVGES GTAKSVTIAN 2600
    YLAHLDSTIN IVLNVNFSSR TSSLDVQRAI EDSTEKRTKD TYGPPMGKRL 2650
    LMFIDDLNMP RVDTYGTQQP IALLKLFIER KGLYDRGKEL SWKNMKDVQV 2700
    VGAMGPPGGA RNPVDPRFIS LFSVFEIQFP SNENLRTIYQ AILSRHLAKL 2750
    PTDEIRDQLG ERLTDVTLEL YNFIIDKLPP TPSRFHYIFN LRDLSRIYEG 2800
    LLLTVGDVFK TPEQFLRLWR NECLRVLHDR LISTDDKRVM TERLEALVQQ 2850
    KFPNLAAHTL ASPVLFGDFK NVINELQGEG EVAPRMYDDL GDYNSIKPLF 2900
    EDVMTNFYNR KRKPMNLVFF EDALEHLTRI HRTLRLPQGN CLLVGVGGSG 2950
    KQSLSKLAAF TAGCEVFEIT LTRGYDELAF REDLKRLYAM LGSDNKRVMF 3000
    LFTDAHVADE GFLELINNML TSGMVPALYD GAEKDGLIGS VRAEVEKKGL 3050
    LATKESCWSY YVDKCRNNLH VVLAMSPVGE TLRSRCRNFP GMVNNTVIDW 3100
    FEPWPEQALT SVASVFLAEE ALPEALRPQI VEHMVTVHQS VRTFSTRFLE 3150
    ELRRYNYVTP KNYLDFINNY KRALATNRRT IEDTVTRLSG GLEKLIQAAV 3200
    EVDAMQKELS QAQVVVAQAT KECNELLEVI STNTVDVETK AKAAAIKEAQ 3250
    LKVDSEQIAI EKAEAEAALE EAIPALEEAA AALQDLSKDH ITEIRSYAKP 3300
    PEQVQKVCEC VVILRNIKDV SWLGAKSMMA DGNFLRSLVE FDKDSLTDKQ 3350
    VKKVKEYFKD PKAPLTYDSL RAISTAGAGL LKWVLAMVNY NNVARTVEPK 3400
    RKKVAESEKN LRIAQKDLAS TKLELQSLND QLGKLRTQFE EKTAEQQDLK 3450
    AKADLMERRL IAASKLIAGL GSERERWTRD IADLESRRDR LIGDCLLTSS 3500
    FLSYTGAFTA TYRHAMVYEM WQDDVKARGV PVTQPFRLEA LLTSDVETTG 3550
    WASEGLPSDE LSIQNGILTV RANRWPLCID PQMQAVNWIK SREGKMLEGK 3600
    VKTFNDSDFL KQLELSIQYG FPFLFENLDE YIDPVIDPVL EKNLVPGDGK 3650
    FVIKLGDKEV EWDSNFRLYM TSKLSNPHYG PEISGKTMII NYGVTQQGLT 3700
    EQLLNVTLRH ERSDLEEARE ALIKQMSENK ATLQALEDTL LRELSNAQGN 3750
    ILDNSELIAT LESAKLKAVE IAEKLEASKV TAAEIEETRV RYSPAAKRGA 3800
    ILFFVIAGLS AITNMYEYSL ASFLVVFNGS LHSSRRDASI EGRLRNIIDT 3850
    LTYDVYAYTC LGLFERHKLM FSFQMTCKIL EGDTPLDPQL LDFFLKGNLS 3900
    LEKAARRKPF DWFPDAGWQD LMRLVELGQK KIGADGRMHA LGSLANDVES 3950
    DEAAWRTWYD LEAPEEAELP CGYQSFLSDF EKLCLMRCLR MDRVTVGITR 4000
    FVIGVMGEKY VQPPVLEYRS IYKQSTETTP IVFVLSPGAD PAFDVFKLGE 4050
    EMGFRPGAKL KYMALGQGMG PKAQELIETG ATRGLWIMLQ NCHLLPTWLK 4100
    TLEKILEKIT KPHADFRLWL TTELTDRFPL GVLQRSLKVV TEPPNGLKLN 4150
    MRQSYSKITE EVLADCPHQA FRPLVYVLGF FHAVVQERRK YGKLGWNVPY 4200
    DFNETDFRIS MALISTYLTK AWDAQDDLIP WGTLRYLIGE AMYGGRVSDS 4250
    YDRRILTTYL DEYLGDFLFD TFQPFRFYAC KDYEIAIPQT GSRDTYLKAV 4300
    EALPLVQSPE AFGLNANADI SYYTSATKAI WTDLVDLQPR TGGGGGGVAR 4350
    EEFIGGVARD IAAKIPEPFD LPQLRKELGT PSPTQVVLLQ ELERWNSVLG 4400
    VMVSSLRDLQ RALSGEIGFS SRLEELASSL YNGKLPAMWA RLNPATEKAL 4450
    GAWMLWFGRR YRQYKDWTEH GEPKVIWLSG LHIPETYIAA LVQAACRDKG 4500
    WPLDKSTLYT KVTKFTDPYQ VSERPKYGCY MSGLYLEGAA WDLEASQLRK 4550
    QDPKVLVNEL PILQVIPIEA NKLKLANTFR APVYVTQARR NAMGVGLVFD 4600
    ADLASAEHSS HWVLQGVALV LNIDQ 4625
    Length:4,625
    Mass (Da):522,843
    Last modified:May 1, 2000 - v1
    Checksum:i875F51048AF99340
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ243806 Genomic DNA. Translation: CAB56598.1.
    U61364 Genomic DNA. Translation: AAC49514.1.
    PIRiS72239.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The kink behind the wriggle - Issue 34 of May 2003

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ243806 Genomic DNA. Translation: CAB56598.1 .
    U61364 Genomic DNA. Translation: AAC49514.1 .
    PIRi S72239.

    3D structure databases

    ProteinModelPortali Q9SMH3.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9SMH3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG5245.

    Family and domain databases

    Gene3Di 3.40.50.300. 4 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR011704. ATPase_dyneun-rel_AAA.
    IPR026983. DHC_fam.
    IPR024743. Dynein_HC_stalk.
    IPR024317. Dynein_heavy_chain_D4_dom.
    IPR004273. Dynein_heavy_dom.
    IPR013594. Dynein_heavy_dom-1.
    IPR013602. Dynein_heavy_dom-2.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10676. PTHR10676. 1 hit.
    Pfami PF07728. AAA_5. 1 hit.
    PF12780. AAA_8. 1 hit.
    PF08385. DHC_N1. 1 hit.
    PF08393. DHC_N2. 1 hit.
    PF03028. Dynein_heavy. 1 hit.
    PF12777. MT. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 4 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 6 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Domains in the 1-alpha dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas."
      Myster S.H., Knott J.A., Wysocki K.M., O'Toole E.T., Porter M.E.
      J. Cell Biol. 146:801-818(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, MUTAGENESIS.
      Strain: 21gr / CC-1690.
    2. "The dynein gene family in Chlamydomonas reinhardtii."
      Porter M.E., Knott J.A., Myster S.H., Farlow S.J.
      Genetics 144:569-585(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1914-2064.
      Strain: 137c / CC-125.
    3. "High-pressure liquid chromatography fractionation of Chlamydomonas dynein extracts and characterization of inner-arm dynein subunits."
      Goodenough U.W., Gebhart B., Mermall V., Mitchell D.R., Heuser J.E.
      J. Mol. Biol. 194:481-494(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: DYNEIN COMPLEX ELECTRON MICROSCOPY.
      Strain: CC-620.
    4. "Three distinct inner dynein arms in Chlamydomonas flagella: molecular composition and location in the axoneme."
      Piperno G., Ramanis Z., Smith E.F., Sale W.S.
      J. Cell Biol. 110:379-389(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    5. "Phosphoregulation of an inner dynein arm complex in Chlamydomonas reinhardtii is altered in phototactic mutant strains."
      King S.J., Dutcher S.K.
      J. Cell Biol. 136:177-191(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: REQUIREMENT OF I1 DYNEIN COMPLEX FOR PHOTOTAXIS.
    6. "The Chlamydomonas Dhc1 gene encodes a dynein heavy chain subunit required for assembly of the I1 inner arm complex."
      Myster S.H., Knott J.A., O'Toole E.T., Porter M.E.
      Mol. Biol. Cell 8:607-620(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOLATION, DISRUPTION PHENOTYPE.
      Strain: 21gr / CC-1690.

    Entry informationi

    Entry nameiDYH1A_CHLRE
    AccessioniPrimary (citable) accession number: Q9SMH3
    Secondary accession number(s): Q96388
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 9, 2003
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3