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Q9SMH3 (DYH1A_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dynein-1-alpha heavy chain, flagellar inner arm I1 complex
Alternative name(s):
1-alpha DHC
Dynein-1, subspecies f
Gene names
Name:DHC1
Synonyms:IDA1, PF9
OrganismChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifier3055 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Protein attributes

Sequence length4625 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Force generating protein of eukaryotic cilia and flagella. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for assembly of the I1 inner arm complex and its targeting to the appropriate axoneme location. Also required for phototaxis.

Subunit structure

The I1 inner arm complex (also known as the f dynein complex) is a two-headed isoform composedof two heavy chains (1-alpha and 1-beta), three intermediate chains and three light chains. I1 occupies a specific position proximal to the first radial spoke and repeats every 96 nm along the length of the axoneme. Ref.4

Subcellular location

Cell projectionciliumflagellum. Cytoplasmcytoskeletonflagellum axoneme.

Induction

By deflagellation.

Domain

Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.

A construct expressing the first 1249 amino acids but lacking the motor domain is able to assemble I1 complexes and target them to their correct location on the axoneme, although motility is not normal. Phototaxis is also restored.

Disruption phenotype

Cells swim slowly with near normal beat frequencies but aberrant waveforms, and are unable to phototax. Ref.6

Sequence similarities

Belongs to the dynein heavy chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 46254625Dynein-1-alpha heavy chain, flagellar inner arm I1 complex
PRO_0000114646

Regions

Nucleotide binding960 – 9678ATP Potential
Nucleotide binding1958 – 19658ATP Potential
Nucleotide binding2242 – 22498ATP Potential
Nucleotide binding2588 – 25958ATP Potential
Nucleotide binding2945 – 29528ATP Potential
Nucleotide binding3680 – 36878ATP Potential
Region1 – 19191919Stem By similarity
Region1920 – 2141222AAA 1 By similarity
Region2201 – 2437237AAA 2 By similarity
Region2550 – 2800251AAA 3 By similarity
Region2906 – 3155250AAA 4 By similarity
Region3192 – 3494303Stalk By similarity
Region3542 – 3773232AAA 5 By similarity
Region3998 – 4216219AAA 6 By similarity
Coiled coil1227 – 125933 Potential
Coiled coil1339 – 140971 Potential
Coiled coil3192 – 3297106 Potential
Coiled coil3400 – 349495 Potential
Coiled coil3701 – 378888 Potential

Sequences

Sequence LengthMass (Da)Tools
Q9SMH3 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 875F51048AF99340

FASTA4,625522,843
        10         20         30         40         50         60 
MDRRLEWVKE KACLGLGVEP NLFEAAIANP ESRARVTAFL DGTVTSSALL FALEEATIYV 

        70         80         90        100        110        120 
EEYQEVLAEE QAPEAEDGEG EEHDGQEPGE AGGEGAEGST APGDSGDGQP EDAPAAAAEA 

       130        140        150        160        170        180 
NGANPEDEAA APADGAADGA AGEGGEEGDG AEGDEPPAPP APKYVRRVIS VPKVVSKLNV 

       190        200        210        220        230        240 
ALGSLPEELS VFPVFYFILN RSGHVAAEEL DSAVEFGLLS EGPSLRILEQ MLSSVFVPIL 

       250        260        270        280        290        300 
VQMSGGDVAS GGVLMQSMTD NSHRELLGNM QKFHSQVTQA LQQLTGDVTL QLPDFPLEDM 

       310        320        330        340        350        360 
DRAAADTDLV MQLEQYMAEW SQVLASVLQR ESQKHPTGKG PLAEIEFWRE RNAVLSSLYE 

       370        380        390        400        410        420 
QLNLPQVKRM ILVVEKGSDD RNLMAGFKSQ LGELTKLATE ARDNVKFLTT LERHFKNIAT 

       430        440        450        460        470        480 
GPLGGILDTL PPMMNALRMV WIISRHYSDD QRMGSLFQRI AREIGDRVEA AVDLRHIFRM 

       490        500        510        520        530        540 
TSADAVELLK VCKSVLEHWL QTYMAMREKI ELSGRDARWE FPKQLLFART NYMAEICTDL 

       550        560        570        580        590        600 
IEMVEIVDDF FRFLGPELKT VTGDTAGIDR VVHRVVAMYE PIESISFNVF DYGNQHEWKA 

       610        620        630        640        650        660 
AKQQFYADNE DIKEATRELI DTSFRKLRSA EGACELLQSF KSIKSKGAIQ KQVMNKFNDI 

       670        680        690        700        710        720 
LEQFAREIEQ TADIFERNKD APPVTKNQPP VAGAIKWVRS LLERLKRTMA KLLSTEEEII 

       730        740        750        760        770        780 
RTTELGQAVE SKFKSFARSV MLTEKKWFSS WSDSINGVAM QHLKQTIFRR SAATNRVEVN 

       790        800        810        820        830        840 
FHPDLVRLIR ETRYLDRMGF PIPEIALNVA LQEDKFLQWL EGLNSMLFKY YESIDQLTPV 

       850        860        870        880        890        900 
ERELMERKLE ELESCLQPGF TILNWNSLGI TEFIGTCDKA IATFQQLVKQ VQKNSGIIEQ 

       910        920        930        940        950        960 
VVYAIAGAQL VTEPEEGAEV MDLQEFYEDI ERQRLAALES LVKKYRTISP LLGKIEEVVA 

       970        980        990       1000       1010       1020 
GTNSGKSPAL SSYYSFWERA IFNALNTMVL CAMTKLQDMI EQRSKHAEGG RKPPLFKVTV 

      1030       1040       1050       1060       1070       1080 
SLQSVDVVVQ PPMTEVNKAL GRLVRSLVES TKAFVRWMDG TCVETPEQRG ATDDDEPIVF 

      1090       1100       1110       1120       1130       1140 
TFYWDVAANP QVIKTMLNLN QSIQRAITSV NKYAESWRRH QALWKTDKNS VLDKFKARDP 

      1150       1160       1170       1180       1190       1200 
SAAQFEDKLS KYAKMATEIS AQAKDFDQDF IRVSCHALAS SVCDEAQAWV RAIAQTMREL 

      1210       1220       1230       1240       1250       1260 
DAVTESQLRD KIAKYQTALH RPPDTLEELK QVLNTVNTIR GESMVMELRY ADLEERYRTR 

      1270       1280       1290       1300       1310       1320 
LLYATNPEEE SQCAHELASA SQVRALWTEL LNEAEAVDWS LEETKKKFSE TTRSQVSDFA 

      1330       1340       1350       1360       1370       1380 
AITAELWEKF RTTGPGLPTV ELASGLDELH KYESNLADAL RQREQLVLAE KLFGMEITAY 

      1390       1400       1410       1420       1430       1440 
PELAQLESEI RKLAQVYGVY AEHAEAVRQY GGQLWSELDV GKMMAGTEAI LTKLRKLKSL 

      1450       1460       1470       1480       1490       1500 
KLLPVYELVE KEIQGFYNSL PLMKELKSEA LRKRHWTRLM EVTGQEFDMD PKTFTLGNMF 

      1510       1520       1530       1540       1550       1560 
AMQLHKYAEE IGKITNAAVK ELTIESEIRK LADVWREQRF ELGKYMKGPE DRGWVLRSTE 

      1570       1580       1590       1600       1610       1620 
DILVLLEDMG LNLQSMMASP FVRPFLTEVR AWEQKLSLIG ECIEVWMHVQ RKWMYLESIF 

      1630       1640       1650       1660       1670       1680 
VGSDDIRHQL PAEAKRFDNI DRQWQKIMND TAKNTVVLDA CMADGRLDLL KSLSEQLEVC 

      1690       1700       1710       1720       1730       1740 
QKSLSEYLDT KRCAFPRFYF ISDDELLSIL GTSDPTSVQE HMLKLFDNCA ALVFGRGNKT 

      1750       1760       1770       1780       1790       1800 
ITGMVSSEKE GFEFRNVVPI EGAVELWMTN VEAEMRKTLY QITKEGIFFY AKTPRTKWIS 

      1810       1820       1830       1840       1850       1860 
ENLGMVTLVG SQIWWTWETE DVFRRVRDGN KHSMKEFAAK LTGQLSELTS MVRSDLSNEV 

      1870       1880       1890       1900       1910       1920 
RKKVNTLIII DVHARDIIDT YVRDSIVDAR EFAWESQLRF YWDRQQDDIL IRQCTGLFKY 

      1930       1940       1950       1960       1970       1980 
GYEYMGLNGR LVITALTDRC YMTLTTALTY RLGGAPAGPA GTGKTETTKD LAKSMALLCV 

      1990       2000       2010       2020       2030       2040 
VFNCGEGLDY KAMGSIFSGL VQCGAWGCFD EFNRIEAEVL SVVSSQIKNI QEALKNDLTR 

      2050       2060       2070       2080       2090       2100 
FQFEGKEISI DPRTGIFITM NPGYAGRTEL PDNLKALFRP VTMVVPDLEQ ICEIMLFSEG 

      2110       2120       2130       2140       2150       2160 
FDSAKVLAKK MTVLYKLSRE QLSKQHHYDF GLRALKSVLV MAGSLKRGAP DMSEQLVLMR 

      2170       2180       2190       2200       2210       2220 
ALRDMNLPKF IFDDVPLFLG LINDLFPGMD CPRVRYPQFN DVVEADLADQ GFKVLTEPSE 

      2230       2240       2250       2260       2270       2280 
QVDKVVQLYE VMMTRHTTMV VGQTGGGKTV ILNTLARAQT KLGKKTHLYT INPKAISVAE 

      2290       2300       2310       2320       2330       2340 
LYGVLDKDTR DWTDGLLSNI FREMNKPLPA ERDEARYLVF DGDVDAVWVE NMNSVMDDNK 

      2350       2360       2370       2380       2390       2400 
LLTLPNGERI RLQNHCKLLF EVFDLQYASP ATISRCGMVY VDSRNLGYKP YIYTWLNSRA 

      2410       2420       2430       2440       2450       2460 
KQAEVDILRG LFEKYAVPSV DWILEGIDGE ELVRRPKQAV PVTNLNMITQ LCNLLNATIT 

      2470       2480       2490       2500       2510       2520 
DHPRMSDPQI LEAIFIFCTI WSLGAAIVQR PESPDRDRFD AFVKHIASMG LVDGERVAAT 

      2530       2540       2550       2560       2570       2580 
QLPARSLYEY CFDTNEGVWK SWRSYLQPYE PPADGAFAKI LVPTVDVVRS TWLLNTVVAA 

      2590       2600       2610       2620       2630       2640 
GKPCLFVGES GTAKSVTIAN YLAHLDSTIN IVLNVNFSSR TSSLDVQRAI EDSTEKRTKD 

      2650       2660       2670       2680       2690       2700 
TYGPPMGKRL LMFIDDLNMP RVDTYGTQQP IALLKLFIER KGLYDRGKEL SWKNMKDVQV 

      2710       2720       2730       2740       2750       2760 
VGAMGPPGGA RNPVDPRFIS LFSVFEIQFP SNENLRTIYQ AILSRHLAKL PTDEIRDQLG 

      2770       2780       2790       2800       2810       2820 
ERLTDVTLEL YNFIIDKLPP TPSRFHYIFN LRDLSRIYEG LLLTVGDVFK TPEQFLRLWR 

      2830       2840       2850       2860       2870       2880 
NECLRVLHDR LISTDDKRVM TERLEALVQQ KFPNLAAHTL ASPVLFGDFK NVINELQGEG 

      2890       2900       2910       2920       2930       2940 
EVAPRMYDDL GDYNSIKPLF EDVMTNFYNR KRKPMNLVFF EDALEHLTRI HRTLRLPQGN 

      2950       2960       2970       2980       2990       3000 
CLLVGVGGSG KQSLSKLAAF TAGCEVFEIT LTRGYDELAF REDLKRLYAM LGSDNKRVMF 

      3010       3020       3030       3040       3050       3060 
LFTDAHVADE GFLELINNML TSGMVPALYD GAEKDGLIGS VRAEVEKKGL LATKESCWSY 

      3070       3080       3090       3100       3110       3120 
YVDKCRNNLH VVLAMSPVGE TLRSRCRNFP GMVNNTVIDW FEPWPEQALT SVASVFLAEE 

      3130       3140       3150       3160       3170       3180 
ALPEALRPQI VEHMVTVHQS VRTFSTRFLE ELRRYNYVTP KNYLDFINNY KRALATNRRT 

      3190       3200       3210       3220       3230       3240 
IEDTVTRLSG GLEKLIQAAV EVDAMQKELS QAQVVVAQAT KECNELLEVI STNTVDVETK 

      3250       3260       3270       3280       3290       3300 
AKAAAIKEAQ LKVDSEQIAI EKAEAEAALE EAIPALEEAA AALQDLSKDH ITEIRSYAKP 

      3310       3320       3330       3340       3350       3360 
PEQVQKVCEC VVILRNIKDV SWLGAKSMMA DGNFLRSLVE FDKDSLTDKQ VKKVKEYFKD 

      3370       3380       3390       3400       3410       3420 
PKAPLTYDSL RAISTAGAGL LKWVLAMVNY NNVARTVEPK RKKVAESEKN LRIAQKDLAS 

      3430       3440       3450       3460       3470       3480 
TKLELQSLND QLGKLRTQFE EKTAEQQDLK AKADLMERRL IAASKLIAGL GSERERWTRD 

      3490       3500       3510       3520       3530       3540 
IADLESRRDR LIGDCLLTSS FLSYTGAFTA TYRHAMVYEM WQDDVKARGV PVTQPFRLEA 

      3550       3560       3570       3580       3590       3600 
LLTSDVETTG WASEGLPSDE LSIQNGILTV RANRWPLCID PQMQAVNWIK SREGKMLEGK 

      3610       3620       3630       3640       3650       3660 
VKTFNDSDFL KQLELSIQYG FPFLFENLDE YIDPVIDPVL EKNLVPGDGK FVIKLGDKEV 

      3670       3680       3690       3700       3710       3720 
EWDSNFRLYM TSKLSNPHYG PEISGKTMII NYGVTQQGLT EQLLNVTLRH ERSDLEEARE 

      3730       3740       3750       3760       3770       3780 
ALIKQMSENK ATLQALEDTL LRELSNAQGN ILDNSELIAT LESAKLKAVE IAEKLEASKV 

      3790       3800       3810       3820       3830       3840 
TAAEIEETRV RYSPAAKRGA ILFFVIAGLS AITNMYEYSL ASFLVVFNGS LHSSRRDASI 

      3850       3860       3870       3880       3890       3900 
EGRLRNIIDT LTYDVYAYTC LGLFERHKLM FSFQMTCKIL EGDTPLDPQL LDFFLKGNLS 

      3910       3920       3930       3940       3950       3960 
LEKAARRKPF DWFPDAGWQD LMRLVELGQK KIGADGRMHA LGSLANDVES DEAAWRTWYD 

      3970       3980       3990       4000       4010       4020 
LEAPEEAELP CGYQSFLSDF EKLCLMRCLR MDRVTVGITR FVIGVMGEKY VQPPVLEYRS 

      4030       4040       4050       4060       4070       4080 
IYKQSTETTP IVFVLSPGAD PAFDVFKLGE EMGFRPGAKL KYMALGQGMG PKAQELIETG 

      4090       4100       4110       4120       4130       4140 
ATRGLWIMLQ NCHLLPTWLK TLEKILEKIT KPHADFRLWL TTELTDRFPL GVLQRSLKVV 

      4150       4160       4170       4180       4190       4200 
TEPPNGLKLN MRQSYSKITE EVLADCPHQA FRPLVYVLGF FHAVVQERRK YGKLGWNVPY 

      4210       4220       4230       4240       4250       4260 
DFNETDFRIS MALISTYLTK AWDAQDDLIP WGTLRYLIGE AMYGGRVSDS YDRRILTTYL 

      4270       4280       4290       4300       4310       4320 
DEYLGDFLFD TFQPFRFYAC KDYEIAIPQT GSRDTYLKAV EALPLVQSPE AFGLNANADI 

      4330       4340       4350       4360       4370       4380 
SYYTSATKAI WTDLVDLQPR TGGGGGGVAR EEFIGGVARD IAAKIPEPFD LPQLRKELGT 

      4390       4400       4410       4420       4430       4440 
PSPTQVVLLQ ELERWNSVLG VMVSSLRDLQ RALSGEIGFS SRLEELASSL YNGKLPAMWA 

      4450       4460       4470       4480       4490       4500 
RLNPATEKAL GAWMLWFGRR YRQYKDWTEH GEPKVIWLSG LHIPETYIAA LVQAACRDKG 

      4510       4520       4530       4540       4550       4560 
WPLDKSTLYT KVTKFTDPYQ VSERPKYGCY MSGLYLEGAA WDLEASQLRK QDPKVLVNEL 

      4570       4580       4590       4600       4610       4620 
PILQVIPIEA NKLKLANTFR APVYVTQARR NAMGVGLVFD ADLASAEHSS HWVLQGVALV 


LNIDQ 

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References

[1]"Domains in the 1-alpha dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas."
Myster S.H., Knott J.A., Wysocki K.M., O'Toole E.T., Porter M.E.
J. Cell Biol. 146:801-818(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, MUTAGENESIS.
Strain: 21gr / CC-1690.
[2]"The dynein gene family in Chlamydomonas reinhardtii."
Porter M.E., Knott J.A., Myster S.H., Farlow S.J.
Genetics 144:569-585(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1914-2064.
Strain: 137c / CC-125.
[3]"High-pressure liquid chromatography fractionation of Chlamydomonas dynein extracts and characterization of inner-arm dynein subunits."
Goodenough U.W., Gebhart B., Mermall V., Mitchell D.R., Heuser J.E.
J. Mol. Biol. 194:481-494(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: DYNEIN COMPLEX ELECTRON MICROSCOPY.
Strain: CC-620.
[4]"Three distinct inner dynein arms in Chlamydomonas flagella: molecular composition and location in the axoneme."
Piperno G., Ramanis Z., Smith E.F., Sale W.S.
J. Cell Biol. 110:379-389(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[5]"Phosphoregulation of an inner dynein arm complex in Chlamydomonas reinhardtii is altered in phototactic mutant strains."
King S.J., Dutcher S.K.
J. Cell Biol. 136:177-191(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: REQUIREMENT OF I1 DYNEIN COMPLEX FOR PHOTOTAXIS.
[6]"The Chlamydomonas Dhc1 gene encodes a dynein heavy chain subunit required for assembly of the I1 inner arm complex."
Myster S.H., Knott J.A., O'Toole E.T., Porter M.E.
Mol. Biol. Cell 8:607-620(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOLATION, DISRUPTION PHENOTYPE.
Strain: 21gr / CC-1690.

Web resources

Protein Spotlight

The kink behind the wriggle - Issue 34 of May 2003

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ243806 Genomic DNA. Translation: CAB56598.1.
U61364 Genomic DNA. Translation: AAC49514.1.
PIRS72239.

3D structure databases

ProteinModelPortalQ9SMH3.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9SMH3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG5245.

Family and domain databases

Gene3D3.40.50.300. 4 hits.
InterProIPR003593. AAA+_ATPase.
IPR011704. ATPase_dyneun-rel_AAA.
IPR026982. DHC1.
IPR026983. DHC_fam.
IPR024743. Dynein_HC_stalk.
IPR024317. Dynein_heavy_chain_D4_dom.
IPR004273. Dynein_heavy_dom.
IPR013594. Dynein_heavy_dom-1.
IPR013602. Dynein_heavy_dom-2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10676. PTHR10676. 1 hit.
PTHR10676:SF135. PTHR10676:SF135. 1 hit.
PfamPF07728. AAA_5. 1 hit.
PF12780. AAA_8. 1 hit.
PF08385. DHC_N1. 1 hit.
PF08393. DHC_N2. 1 hit.
PF03028. Dynein_heavy. 1 hit.
PF12777. MT. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 4 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 6 hits.
ProtoNetSearch...

Entry information

Entry nameDYH1A_CHLRE
AccessionPrimary (citable) accession number: Q9SMH3
Secondary accession number(s): Q96388
Entry history
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries