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Protein

Monogalactosyldiacylglycerol synthase, chloroplastic

Gene

MGD A

Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the major structural component of photosynthetic membranes. The 1,2-diacylglycerol substrate preference is 18:2/18:2 > 18:0/18:1 > 18:1/18:1 > 18:1/16:0 > 16:0/18:2 > 18:3/18:3 > 16:0/18:1 > 16:0/16:0 > 18:0/18:0.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

Cofactori

Zn2+Curated

Enzyme regulationi

Inhibited by ortho-phenanthroline and UDP (competitive inhibitor relatively to UDP-Gal only) and inactivated by citraconic anhydride, tert-butoxycarbonyl-L-methionine hydrosuccinimidyl ester (SLR) and N-ethylmaleimide (NEM).2 Publications

Kineticsi

  1. KM=0.65 mM for UDP-Gal1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei422Substrate specificity for galactose1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.46. 5812.

    Protein family/group databases

    CAZyiGT28. Glycosyltransferase Family 28.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monogalactosyldiacylglycerol synthase, chloroplastic (EC:2.4.1.46)
    Short name:
    SoMGD1
    Alternative name(s):
    MGDG synthase type A
    Gene namesi
    Name:MGD A
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Plastid inner membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi171W → A: 90% reduction of activity. 1 Publication1
    Mutagenesisi173E → N: 90% reduction of activity. 1 Publication1
    Mutagenesisi177W → A: 90% reduction of activity. 1 Publication1
    Mutagenesisi240H → A: Total loss of activity. 1 Publication1
    Mutagenesisi245H → A: 90% reduction of activity. 1 Publication1
    Mutagenesisi284C → A: No effect. 1 Publication1
    Mutagenesisi286C → A: No effect. 1 Publication1
    Mutagenesisi343 – 347Missing : Total loss of activity. 1 Publication5
    Mutagenesisi346E → A: 90% reduction of activity. 1 Publication1
    Mutagenesisi378C → A: 25% reduction of activity. 1 Publication1
    Mutagenesisi380R → E: 90% reduction of activity. 1 Publication1
    Mutagenesisi402F → A: 90% reduction of activity. 1 Publication1
    Mutagenesisi415C → A: No effect. 1 Publication1
    Mutagenesisi419K → A: Total loss of activity. 1 Publication1
    Mutagenesisi427E → A: Total loss of activity. 1 Publication1
    Mutagenesisi445E → A: Total loss of activity. 1 Publication1
    Mutagenesisi448N → A: 90% reduction of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 98ChloroplastSequence analysisAdd BLAST98
    ChainiPRO_500006567499 – 522Monogalactosyldiacylglycerol synthase, chloroplasticAdd BLAST424

    Interactioni

    Subunit structurei

    Homodimer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SM44.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The C-terminal domain (308-483) is involved in UDP-Gal binding while the N-terminal domains (131-307) is involved in dyacylglycerol binding.

    Sequence similaritiesi

    Belongs to the glycosyltransferase 28 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03715.

    Family and domain databases

    InterProiIPR009695. Diacylglyc_glucosyltr_N.
    IPR007235. Glyco_trans_28_C.
    [Graphical view]
    PfamiPF04101. Glyco_tran_28_C. 1 hit.
    PF06925. MGDG_synth. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SM44-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSHPSTVTSE PSNLLDFVPK LGNFVLNSSL HGNNSNGYSS FSSNSVHFGG
    60 70 80 90 100
    LATQNRYKFV NSLSFSKEGS NLKRILSDFN RVIRLHCDRI PLGFSSIGLN
    110 120 130 140 150
    SGESNGVSDN GHGVLEDVRV PVNAVEPESP KRVLILMSDT GGGHRASAEA
    160 170 180 190 200
    IKAAFNEEFG DDYQVFVTDL WSEHTPWPFN QLPRSYNFLV KHGPLWKMMY
    210 220 230 240 250
    YGTSPRVIHQ SNFAATSVFI AREVARGLMK YQPDIIISVH PLMQHVPLRI
    260 270 280 290 300
    LRGRGLLEKI VFTTVVTDLS TCHPTWFHKL VTRCYCPSNE VAKRATKAGL
    310 320 330 340 350
    QPSQIKVYGL PVRPSFVRSV RPKNELRKEL GMDEHLPAVL LMGGGEGMGP
    360 370 380 390 400
    IEATARALGN ALYDANLGEP TGQLLVICGR NKKLAGKLSS IDWKIPVQVK
    410 420 430 440 450
    GFVTKIEECM GACDCIITKA GPGTIAEAMI RGLPIILNDY IAGQEAGNVP
    460 470 480 490 500
    YVIENGIGKY LKSPKEIAKT VSQWFGPKAN ELQIMSQNAL KHARPDAVFK
    510 520
    IVHDLDELVR QKIFVRQYSC AA
    Length:522
    Mass (Da):57,511
    Last modified:May 1, 2000 - v1
    Checksum:i02E2B929732551A7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ249607 mRNA. Translation: CAB56218.1.

    Genome annotation databases

    KEGGiag:CAB56218.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ249607 mRNA. Translation: CAB56218.1.

    3D structure databases

    ProteinModelPortaliQ9SM44.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiGT28. Glycosyltransferase Family 28.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:CAB56218.

    Phylogenomic databases

    KOiK03715.

    Enzyme and pathway databases

    BRENDAi2.4.1.46. 5812.

    Family and domain databases

    InterProiIPR009695. Diacylglyc_glucosyltr_N.
    IPR007235. Glyco_trans_28_C.
    [Graphical view]
    PfamiPF04101. Glyco_tran_28_C. 1 hit.
    PF06925. MGDG_synth. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMGDG_SPIOL
    AccessioniPrimary (citable) accession number: Q9SM44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: May 1, 2000
    Last modified: July 6, 2016
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.