Q9SLZ0 (PCKA_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phosphoenolpyruvate carboxykinase [ATP] Short name=PEP carboxykinase Short name=PEPCK EC=4.1.1.49 Alternative name(s): Phosphoenolpyruvate carboxylase
Organism	Zea mays (Maize)
Taxonomic identifier	4577 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	666 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Gene Ontology (GO)
Biological_process	cellular response to phosphate starvation Inferred from electronic annotation. Source: EnsemblPlants/Gramene defense response to fungus, incompatible interaction Inferred from electronic annotation. Source: EnsemblPlants/Gramene gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-UniPathway response to cadmium ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	cytosol Inferred from electronic annotation. Source: EnsemblPlants/Gramene membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene nucleolus Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 666	666	Phosphoenolpyruvate carboxykinase [ATP]		PRO_0000203864
Regions
	Nucleotide binding	364 – 371	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9SLZ0 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 813392EF13BF91D3	FASTA	666	73,314
	10 20 30 40 50 60 MATPNGLARI ETTGKKKQDN GVWYDDSSAP VRAQTIDELH SLQRKRSAPT TPNRSAPTTP 70 80 90 100 110 120 IKGGAHSPFA VAISEEERHT QQMQSISASL ASLTRETGPK VVKGDPAAKG EAAAQGAPST 130 140 150 160 170 180 PRAHQQHRHP AAPAIAVSDS SLKFTHVLNN LSPAELYEQA IKYEKGSFIT STGALATLSG 190 200 210 220 230 240 AKTGRSPRDK RVVKDEVTAQ DLWWGKGSPN IEMDEKTFLI NRERAVDYLN SLKVVRQRQL 250 260 270 280 290 300 LNWDPENRIK VRIISARAYH SLFMHNMCIR PTDEELEDFG TPDFTIYNAG QFPCNRYTHY 310 320 330 340 350 360 MTSSTSIDLN LARREMVIMG TQYAGEMKKG LFGVMHYLMP KRGILSLHSG CNMGKDGDVA 370 380 390 400 410 420 LFFGLSGTGK TTLSTDHNGL LIGDDEHCWS DNGVSNIEGG CYAKCIDLAQ EKEPDIWNAI 430 440 450 460 470 480 KFGTVLENVV FDEHTREVDY ADYSVTENTR AAYPIEYIPI AKIPCVGPHP KNVILLACDA 490 500 510 520 530 540 FGVLPPVSKL ILAQTMYHFI SGYTALVAGT EDGIKEPQAT FSACFGAAFI MLHPTKYAAM 550 560 570 580 590 600 LAEKMQKYGA TGWLVNTGWS GGRYGVGKRI RLPYTRKIID AIHSGELLTA NYQKTEVFGL 610 620 630 640 650 660 EIPTEINGVP SEILDPIYTW TDKAAYKENL LRLGGLFKNN FEVFASYKIG DDSSLTDEIL AAGPNF

References

[1]

"cDNA cloning and characterization of maize phosphoenolpyruvate carboxykinase, a bundle sheath cell-specific enzyme." Furumoto T., Hata S., Izui K. Plant Mol. Biol. 41:301-311(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. H84. Tissue: Bundle sheath strand.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB018744 mRNA. Translation: BAA36483.1.
RefSeq	NP_001104847.1. NM_001111377.1.
UniGene	Zm.32453.
3D structure databases
ProteinModelPortal	Q9SLZ0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	541622.
KEGG	zma:541622.
Organism-specific databases
Gramene	Q9SLZ0.
Phylogenomic databases
KO	K01610.
Enzyme and pathway databases
UniPathway	UPA00138.
Family and domain databases
Gene3D	3.40.449.10. 1 hit. 3.90.228.20. 2 hits.
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. IPR015994. PEPCK_ATP_CS. [Graphical view]
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMs	TIGR00224. pckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA_MAIZE

Accession

Primary (citable) accession number: Q9SLZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program