Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9SLY8

- CALR_ORYSJ

UniProt

Q9SLY8 - CALR_ORYSJ

Protein

Calreticulin

Gene

Os07g0246200

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei119 – 1191CarbohydrateBy similarity
    Binding sitei121 – 1211CarbohydrateBy similarity
    Binding sitei138 – 1381CarbohydrateBy similarity
    Binding sitei145 – 1451CarbohydrateBy similarity
    Binding sitei328 – 3281CarbohydrateBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: Gramene
    2. unfolded protein binding Source: Gramene

    GO - Biological processi

    1. multicellular organismal development Source: Gramene
    2. protein folding Source: InterPro

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    Calcium, Lectin, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calreticulin
    Gene namesi
    Ordered Locus Names:Os07g0246200, LOC_Os07g14270
    ORF Names:OJ1058_C08.34-1, OJ1058_C08.34-2, OSJNBa0003K21.18-1, OSJNBa0003K21.18-2
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 7

    Organism-specific databases

    GrameneiQ9SLY8.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: Gramene

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 29291 PublicationAdd
    BLAST
    Chaini30 – 424395CalreticulinPRO_0000004193Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi115 ↔ 147By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9SLY8.
    PRIDEiQ9SLY8.

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os07g14270.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SLY8.
    SMRiQ9SLY8. Positions 216-316.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati201 – 212121-1Add
    BLAST
    Repeati220 – 231121-2Add
    BLAST
    Repeati237 – 248121-3Add
    BLAST
    Repeati255 – 266121-4Add
    BLAST
    Repeati270 – 280112-1Add
    BLAST
    Repeati284 – 294112-2Add
    BLAST
    Repeati298 – 308112-3Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni201 – 266664 X approximate repeatsAdd
    BLAST
    Regioni270 – 308393 X approximate repeatsAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi421 – 4244Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi362 – 42059Asp/Glu/Lys-richAdd
    BLAST

    Domaini

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity.By similarity
    The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
    The zinc binding sites are localized to the N-domain.By similarity

    Sequence similaritiesi

    Belongs to the calreticulin family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG305105.
    HOGENOMiHOG000192435.
    KOiK08057.
    OMAiVKLFPDG.

    Family and domain databases

    Gene3Di2.10.250.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProiIPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009169. Calreticulin.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view]
    PANTHERiPTHR11073. PTHR11073. 1 hit.
    PfamiPF00262. Calreticulin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002356. Calreticulin. 1 hit.
    PRINTSiPR00626. CALRETICULIN.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    SSF63887. SSF63887. 1 hit.
    PROSITEiPS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 2 hits.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9SLY8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAIRARSSSY AAAAVALALA LASVAAVAGE VFFQEKFEDG WESRWVKSEW    50
    KKDENMAGEW NHTSGKWNGD PEDKGIQTSE DYRFYAISAE YPEFSNKDKT 100
    LVLQFSVKHE QKLDCGGGYV KLLGGDVDQK KFGGDTPYSI MFGPDICGYS 150
    TKKVHTIFTK NDKNHLIKKD VPCETDQLSH VYTLIIHPDA TYTILIDNVE 200
    KQSGSIYEHW DILPPKQIKD PEAKKPEDWD DKEYIPDPED KKPEGYDDIP 250
    KEIPDPDAKK PEDWDDEEDG EWTAPTIPNP EYKGPWKQKK IKNPNYQGKW 300
    KAPMIDNPDF KDDPYIYAFD SLKYIGIELW QVKSGTLFDN FLITDDPELA 350
    KTFAEETWGK HKDAEKAAFD EAEKKKEEEE AAKAGEDDDD LDDEDAEDED 400
    KADEKADSDA EDGKDSDDEK HDEL 424
    Length:424
    Mass (Da):48,309
    Last modified:December 20, 2005 - v2
    Checksum:iEFAE5723CB03BAE0
    GO
    Isoform 2 (identifier: Q9SLY8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         422-424: DEL → VCNTPAFS

    Note: No experimental confirmation available.

    Show »
    Length:429
    Mass (Da):48,772
    Checksum:i1BD29FEC132CCBEF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti193 – 1931T → S in BAA88900. (PubMed:10651810)Curated
    Sequence conflicti263 – 2653DWD → AGA in BAA88900. (PubMed:10651810)Curated
    Sequence conflicti278 – 2781P → R in BAA88900. (PubMed:10651810)Curated
    Sequence conflicti302 – 3021A → P in BAA88900. (PubMed:10651810)Curated
    Sequence conflicti306 – 3072DN → AT in BAA88900. (PubMed:10651810)Curated
    Sequence conflicti311 – 3111K → Q in BAA88900. (PubMed:10651810)Curated
    Sequence conflicti324 – 3241Y → S in BAA88900. (PubMed:10651810)Curated
    Sequence conflicti341 – 3411F → I in BAA88900. (PubMed:10651810)Curated
    Sequence conflicti347 – 3482PE → AA in BAA88900. (PubMed:10651810)Curated
    Sequence conflicti359 – 3591G → A in BAA88900. (PubMed:10651810)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei422 – 4243DEL → VCNTPAFS in isoform 2. 1 PublicationVSP_016726

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB021259 mRNA. Translation: BAA88900.1.
    AP003738 Genomic DNA. Translation: BAC82932.1.
    AP003738 Genomic DNA. Translation: BAC82933.1.
    AP006266 Genomic DNA. Translation: BAD31961.1.
    AP006266 Genomic DNA. Translation: BAD31962.1.
    AK065341 mRNA. No translation available.
    AK104328 mRNA. No translation available.
    RefSeqiNP_001059283.1. NM_001065818.1. [Q9SLY8-1]
    UniGeneiOs.60530.

    Genome annotation databases

    EnsemblPlantsiOS07T0246200-01; OS07T0246200-01; OS07G0246200. [Q9SLY8-1]
    OS07T0246200-02; OS07T0246200-02; OS07G0246200. [Q9SLY8-1]
    GeneIDi4342826.
    KEGGiosa:4342826.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB021259 mRNA. Translation: BAA88900.1 .
    AP003738 Genomic DNA. Translation: BAC82932.1 .
    AP003738 Genomic DNA. Translation: BAC82933.1 .
    AP006266 Genomic DNA. Translation: BAD31961.1 .
    AP006266 Genomic DNA. Translation: BAD31962.1 .
    AK065341 mRNA. No translation available.
    AK104328 mRNA. No translation available.
    RefSeqi NP_001059283.1. NM_001065818.1. [Q9SLY8-1 ]
    UniGenei Os.60530.

    3D structure databases

    ProteinModelPortali Q9SLY8.
    SMRi Q9SLY8. Positions 216-316.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os07g14270.1.

    Proteomic databases

    PaxDbi Q9SLY8.
    PRIDEi Q9SLY8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS07T0246200-01 ; OS07T0246200-01 ; OS07G0246200 . [Q9SLY8-1 ]
    OS07T0246200-02 ; OS07T0246200-02 ; OS07G0246200 . [Q9SLY8-1 ]
    GeneIDi 4342826.
    KEGGi osa:4342826.

    Organism-specific databases

    Gramenei Q9SLY8.

    Phylogenomic databases

    eggNOGi NOG305105.
    HOGENOMi HOG000192435.
    KOi K08057.
    OMAi VKLFPDG.

    Family and domain databases

    Gene3Di 2.10.250.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProi IPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009169. Calreticulin.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view ]
    PANTHERi PTHR11073. PTHR11073. 1 hit.
    Pfami PF00262. Calreticulin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002356. Calreticulin. 1 hit.
    PRINTSi PR00626. CALRETICULIN.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    SSF63887. SSF63887. 1 hit.
    PROSITEi PS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 2 hits.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of calreticulin, a calcium-binding protein involved in the regeneration of rice cultured suspension cells."
      Li Z., Komatsu S.
      Eur. J. Biochem. 267:737-745(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: cv. Nipponbare.
    4. "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
      Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
      Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 30-39; 81-90; 94-103; 111-120 AND 245-253.
      Strain: cv. Nipponbare.
      Tissue: Anther, Embryo, Panicle, Sheath and Stem.

    Entry informationi

    Entry nameiCALR_ORYSJ
    AccessioniPrimary (citable) accession number: Q9SLY8
    Secondary accession number(s): Q6ZLM7, Q6ZLM8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3