Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9SLY8

- CALR_ORYSJ

UniProt

Q9SLY8 - CALR_ORYSJ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Calreticulin

Gene

Os07g0246200

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei119 – 1191CarbohydrateBy similarity
Binding sitei121 – 1211CarbohydrateBy similarity
Binding sitei138 – 1381CarbohydrateBy similarity
Binding sitei145 – 1451CarbohydrateBy similarity
Binding sitei328 – 3281CarbohydrateBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: Gramene
  2. carbohydrate binding Source: UniProtKB-KW
  3. unfolded protein binding Source: Gramene

GO - Biological processi

  1. multicellular organismal development Source: Gramene
  2. protein folding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin
Gene namesi
Ordered Locus Names:Os07g0246200, LOC_Os07g14270
ORF Names:OJ1058_C08.34-1, OJ1058_C08.34-2, OSJNBa0003K21.18-1, OSJNBa0003K21.18-2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 7

Organism-specific databases

GrameneiQ9SLY8.

Subcellular locationi

Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: Gramene
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 PublicationAdd
BLAST
Chaini30 – 424395CalreticulinPRO_0000004193Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi115 ↔ 147By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9SLY8.
PRIDEiQ9SLY8.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SLY8. baseline.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os07g14270.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SLY8.
SMRiQ9SLY8. Positions 216-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati201 – 212121-1Add
BLAST
Repeati220 – 231121-2Add
BLAST
Repeati237 – 248121-3Add
BLAST
Repeati255 – 266121-4Add
BLAST
Repeati270 – 280112-1Add
BLAST
Repeati284 – 294112-2Add
BLAST
Repeati298 – 308112-3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni201 – 266664 X approximate repeatsAdd
BLAST
Regioni270 – 308393 X approximate repeatsAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi421 – 4244Prevents secretion from ERPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi362 – 42059Asp/Glu/Lys-richAdd
BLAST

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity.By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG305105.
HOGENOMiHOG000192435.
InParanoidiQ9SLY8.
KOiK08057.
OMAiVKLFPDG.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SLY8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIRARSSSY AAAAVALALA LASVAAVAGE VFFQEKFEDG WESRWVKSEW
60 70 80 90 100
KKDENMAGEW NHTSGKWNGD PEDKGIQTSE DYRFYAISAE YPEFSNKDKT
110 120 130 140 150
LVLQFSVKHE QKLDCGGGYV KLLGGDVDQK KFGGDTPYSI MFGPDICGYS
160 170 180 190 200
TKKVHTIFTK NDKNHLIKKD VPCETDQLSH VYTLIIHPDA TYTILIDNVE
210 220 230 240 250
KQSGSIYEHW DILPPKQIKD PEAKKPEDWD DKEYIPDPED KKPEGYDDIP
260 270 280 290 300
KEIPDPDAKK PEDWDDEEDG EWTAPTIPNP EYKGPWKQKK IKNPNYQGKW
310 320 330 340 350
KAPMIDNPDF KDDPYIYAFD SLKYIGIELW QVKSGTLFDN FLITDDPELA
360 370 380 390 400
KTFAEETWGK HKDAEKAAFD EAEKKKEEEE AAKAGEDDDD LDDEDAEDED
410 420
KADEKADSDA EDGKDSDDEK HDEL
Length:424
Mass (Da):48,309
Last modified:December 20, 2005 - v2
Checksum:iEFAE5723CB03BAE0
GO
Isoform 2 (identifier: Q9SLY8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     422-424: DEL → VCNTPAFS

Note: No experimental confirmation available.

Show »
Length:429
Mass (Da):48,772
Checksum:i1BD29FEC132CCBEF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti193 – 1931T → S in BAA88900. (PubMed:10651810)Curated
Sequence conflicti263 – 2653DWD → AGA in BAA88900. (PubMed:10651810)Curated
Sequence conflicti278 – 2781P → R in BAA88900. (PubMed:10651810)Curated
Sequence conflicti302 – 3021A → P in BAA88900. (PubMed:10651810)Curated
Sequence conflicti306 – 3072DN → AT in BAA88900. (PubMed:10651810)Curated
Sequence conflicti311 – 3111K → Q in BAA88900. (PubMed:10651810)Curated
Sequence conflicti324 – 3241Y → S in BAA88900. (PubMed:10651810)Curated
Sequence conflicti341 – 3411F → I in BAA88900. (PubMed:10651810)Curated
Sequence conflicti347 – 3482PE → AA in BAA88900. (PubMed:10651810)Curated
Sequence conflicti359 – 3591G → A in BAA88900. (PubMed:10651810)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei422 – 4243DEL → VCNTPAFS in isoform 2. 1 PublicationVSP_016726

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB021259 mRNA. Translation: BAA88900.1.
AP003738 Genomic DNA. Translation: BAC82932.1.
AP003738 Genomic DNA. Translation: BAC82933.1.
AP006266 Genomic DNA. Translation: BAD31961.1.
AP006266 Genomic DNA. Translation: BAD31962.1.
AK065341 mRNA. No translation available.
AK104328 mRNA. No translation available.
RefSeqiNP_001059283.1. NM_001065818.1. [Q9SLY8-1]
UniGeneiOs.60530.

Genome annotation databases

EnsemblPlantsiOS07T0246200-01; OS07T0246200-01; OS07G0246200. [Q9SLY8-1]
OS07T0246200-02; OS07T0246200-02; OS07G0246200. [Q9SLY8-1]
GeneIDi4342826.
KEGGiosa:4342826.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB021259 mRNA. Translation: BAA88900.1 .
AP003738 Genomic DNA. Translation: BAC82932.1 .
AP003738 Genomic DNA. Translation: BAC82933.1 .
AP006266 Genomic DNA. Translation: BAD31961.1 .
AP006266 Genomic DNA. Translation: BAD31962.1 .
AK065341 mRNA. No translation available.
AK104328 mRNA. No translation available.
RefSeqi NP_001059283.1. NM_001065818.1. [Q9SLY8-1 ]
UniGenei Os.60530.

3D structure databases

ProteinModelPortali Q9SLY8.
SMRi Q9SLY8. Positions 216-316.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39947.LOC_Os07g14270.1.

Proteomic databases

PaxDbi Q9SLY8.
PRIDEi Q9SLY8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS07T0246200-01 ; OS07T0246200-01 ; OS07G0246200 . [Q9SLY8-1 ]
OS07T0246200-02 ; OS07T0246200-02 ; OS07G0246200 . [Q9SLY8-1 ]
GeneIDi 4342826.
KEGGi osa:4342826.

Organism-specific databases

Gramenei Q9SLY8.

Phylogenomic databases

eggNOGi NOG305105.
HOGENOMi HOG000192435.
InParanoidi Q9SLY8.
KOi K08057.
OMAi VKLFPDG.

Gene expression databases

ExpressionAtlasi Q9SLY8. baseline.

Family and domain databases

Gene3Di 2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProi IPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view ]
PANTHERi PTHR11073. PTHR11073. 1 hit.
Pfami PF00262. Calreticulin. 1 hit.
[Graphical view ]
PIRSFi PIRSF002356. Calreticulin. 1 hit.
PRINTSi PR00626. CALRETICULIN.
SUPFAMi SSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEi PS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of calreticulin, a calcium-binding protein involved in the regeneration of rice cultured suspension cells."
    Li Z., Komatsu S.
    Eur. J. Biochem. 267:737-745(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: cv. Nipponbare.
  4. "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
    Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
    Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-39; 81-90; 94-103; 111-120 AND 245-253.
    Strain: cv. Nipponbare.
    Tissue: Anther, Embryo, Panicle, Sheath and Stem.

Entry informationi

Entry nameiCALR_ORYSJ
AccessioniPrimary (citable) accession number: Q9SLY8
Secondary accession number(s): Q6ZLM7, Q6ZLM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3