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Q9SLY8 (CALR_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calreticulin
Gene names
Ordered Locus Names:Os07g0246200, LOC_Os07g14270
ORF Names:OJ1058_C08.34-1, OJ1058_C08.34-2, OSJNBa0003K21.18-1, OSJNBa0003K21.18-2
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length424 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.

Subcellular location

Endoplasmic reticulum lumen By similarity.

Domain

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity.

The interaction with glycans occurs through a binding site in the globular lectin domain By similarity.

The zinc binding sites are localized to the N-domain By similarity.

Sequence similarities

Belongs to the calreticulin family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9SLY8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9SLY8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     422-424: DEL → VCNTPAFS
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Ref.4
Chain30 – 424395Calreticulin
PRO_0000004193

Regions

Repeat201 – 212121-1
Repeat220 – 231121-2
Repeat237 – 248121-3
Repeat255 – 266121-4
Repeat270 – 280112-1
Repeat284 – 294112-2
Repeat298 – 308112-3
Region201 – 266664 X approximate repeats
Region270 – 308393 X approximate repeats
Motif421 – 4244Prevents secretion from ER Potential
Compositional bias362 – 42059Asp/Glu/Lys-rich

Sites

Binding site1191Carbohydrate By similarity
Binding site1211Carbohydrate By similarity
Binding site1381Carbohydrate By similarity
Binding site1451Carbohydrate By similarity
Binding site3281Carbohydrate By similarity

Amino acid modifications

Glycosylation611N-linked (GlcNAc...) Potential
Disulfide bond115 ↔ 147 By similarity

Natural variations

Alternative sequence422 – 4243DEL → VCNTPAFS in isoform 2.
VSP_016726

Experimental info

Sequence conflict1931T → S in BAA88900. Ref.1
Sequence conflict263 – 2653DWD → AGA in BAA88900. Ref.1
Sequence conflict2781P → R in BAA88900. Ref.1
Sequence conflict3021A → P in BAA88900. Ref.1
Sequence conflict306 – 3072DN → AT in BAA88900. Ref.1
Sequence conflict3111K → Q in BAA88900. Ref.1
Sequence conflict3241Y → S in BAA88900. Ref.1
Sequence conflict3411F → I in BAA88900. Ref.1
Sequence conflict347 – 3482PE → AA in BAA88900. Ref.1
Sequence conflict3591G → A in BAA88900. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 20, 2005. Version 2.
Checksum: EFAE5723CB03BAE0

FASTA42448,309
        10         20         30         40         50         60 
MAIRARSSSY AAAAVALALA LASVAAVAGE VFFQEKFEDG WESRWVKSEW KKDENMAGEW 

        70         80         90        100        110        120 
NHTSGKWNGD PEDKGIQTSE DYRFYAISAE YPEFSNKDKT LVLQFSVKHE QKLDCGGGYV 

       130        140        150        160        170        180 
KLLGGDVDQK KFGGDTPYSI MFGPDICGYS TKKVHTIFTK NDKNHLIKKD VPCETDQLSH 

       190        200        210        220        230        240 
VYTLIIHPDA TYTILIDNVE KQSGSIYEHW DILPPKQIKD PEAKKPEDWD DKEYIPDPED 

       250        260        270        280        290        300 
KKPEGYDDIP KEIPDPDAKK PEDWDDEEDG EWTAPTIPNP EYKGPWKQKK IKNPNYQGKW 

       310        320        330        340        350        360 
KAPMIDNPDF KDDPYIYAFD SLKYIGIELW QVKSGTLFDN FLITDDPELA KTFAEETWGK 

       370        380        390        400        410        420 
HKDAEKAAFD EAEKKKEEEE AAKAGEDDDD LDDEDAEDED KADEKADSDA EDGKDSDDEK 


HDEL

« Hide

Isoform 2 [UniParc].

Checksum: 1BD29FEC132CCBEF
Show »

FASTA42948,772

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of calreticulin, a calcium-binding protein involved in the regeneration of rice cultured suspension cells."
Li Z., Komatsu S.
Eur. J. Biochem. 267:737-745(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Nipponbare.
[4]"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 30-39; 81-90; 94-103; 111-120 AND 245-253.
Strain: cv. Nipponbare.
Tissue: Anther, Embryo, Panicle, Sheath and Stem.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB021259 mRNA. Translation: BAA88900.1.
AP003738 Genomic DNA. Translation: BAC82932.1.
AP003738 Genomic DNA. Translation: BAC82933.1.
AP006266 Genomic DNA. Translation: BAD31961.1.
AP006266 Genomic DNA. Translation: BAD31962.1.
AK065341 mRNA. No translation available.
AK104328 mRNA. No translation available.
RefSeqNP_001059283.1. NM_001065818.1.
UniGeneOs.60530.

3D structure databases

ProteinModelPortalQ9SLY8.
SMRQ9SLY8. Positions 216-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os07g14270.1.

Proteomic databases

PaxDbQ9SLY8.
PRIDEQ9SLY8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS07T0246200-01; OS07T0246200-01; OS07G0246200.
OS07T0246200-02; OS07T0246200-02; OS07G0246200.
GeneID4342826.
KEGGosa:4342826.

Organism-specific databases

GrameneQ9SLY8.

Phylogenomic databases

eggNOGNOG305105.
HOGENOMHOG000192435.
KOK08057.
OMAVKLFPDG.
ProtClustDBCLSN2919439.

Gene expression databases

ArrayExpressQ9SLY8.

Family and domain databases

Gene3D2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view]
PANTHERPTHR11073. PTHR11073. 1 hit.
PfamPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFPIRSF002356. Calreticulin. 1 hit.
PRINTSPR00626. CALRETICULIN.
SUPFAMSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCALR_ORYSJ
AccessionPrimary (citable) accession number: Q9SLY8
Secondary accession number(s): Q6ZLM7, Q6ZLM8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 20, 2005
Last modified: November 13, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents