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UniProtKB - Q9SLY8 (CALR_ORYSJ)

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Protein

Calreticulin

Gene

Os07g0246200

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119CarbohydrateBy similarity1
Binding sitei121CarbohydrateBy similarity1
Binding sitei138CarbohydrateBy similarity1
Binding sitei145CarbohydrateBy similarity1
Binding sitei328CarbohydrateBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: Gramene
  • carbohydrate binding Source: UniProtKB-KW
  • unfolded protein binding Source: Gramene
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • multicellular organism development Source: Gramene
  • protein folding Source: InterPro
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionChaperone
LigandCalcium, Lectin, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-OSA-901042 Calnexin/calreticulin cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin
Gene namesi
Ordered Locus Names:Os07g0246200, LOC_Os07g14270
ORF Names:OJ1058_C08.34-1, OJ1058_C08.34-2, OSJNBa0003K21.18-1, OSJNBa0003K21.18-2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000000419330 – 424CalreticulinAdd BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi61N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi115 ↔ 147By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9SLY8
PRIDEiQ9SLY8

Expressioni

Gene expression databases

ExpressionAtlasiQ9SLY8 differential
GenevisibleiQ9SLY8 OS

Interactioni

GO - Molecular functioni

  • unfolded protein binding Source: Gramene
View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGi39947.LOC_Os07g14270.1

Structurei

3D structure databases

ProteinModelPortaliQ9SLY8
SMRiQ9SLY8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati201 – 2121-1Add BLAST12
Repeati220 – 2311-2Add BLAST12
Repeati237 – 2481-3Add BLAST12
Repeati255 – 2661-4Add BLAST12
Repeati270 – 2802-1Add BLAST11
Repeati284 – 2942-2Add BLAST11
Repeati298 – 3082-3Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni201 – 2664 X approximate repeatsAdd BLAST66
Regioni270 – 3083 X approximate repeatsAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi421 – 424Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi362 – 420Asp/Glu/Lys-richAdd BLAST59

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG0674 Eukaryota
ENOG410XRR7 LUCA
HOGENOMiHOG000192435
InParanoidiQ9SLY8
KOiK08057
OMAiNSKHKSD

Family and domain databases

Gene3Di2.10.250.10, 2 hits
InterProiView protein in InterPro
IPR001580 Calret/calnex
IPR018124 Calret/calnex_CS
IPR009169 Calreticulin
IPR009033 Calreticulin/calnexin_P_dom_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR11073 PTHR11073, 1 hit
PfamiView protein in Pfam
PF00262 Calreticulin, 2 hits
PIRSFiPIRSF002356 Calreticulin, 1 hit
PRINTSiPR00626 CALRETICULIN
SUPFAMiSSF49899 SSF49899, 1 hit
SSF63887 SSF63887, 1 hit
PROSITEiView protein in PROSITE
PS00803 CALRETICULIN_1, 1 hit
PS00804 CALRETICULIN_2, 1 hit
PS00805 CALRETICULIN_REPEAT, 2 hits
PS00014 ER_TARGET, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SLY8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIRARSSSY AAAAVALALA LASVAAVAGE VFFQEKFEDG WESRWVKSEW 
        60         70         80         90        100
KKDENMAGEW NHTSGKWNGD PEDKGIQTSE DYRFYAISAE YPEFSNKDKT 
       110        120        130        140        150
LVLQFSVKHE QKLDCGGGYV KLLGGDVDQK KFGGDTPYSI MFGPDICGYS 
       160        170        180        190        200
TKKVHTIFTK NDKNHLIKKD VPCETDQLSH VYTLIIHPDA TYTILIDNVE 
       210        220        230        240        250
KQSGSIYEHW DILPPKQIKD PEAKKPEDWD DKEYIPDPED KKPEGYDDIP 
       260        270        280        290        300
KEIPDPDAKK PEDWDDEEDG EWTAPTIPNP EYKGPWKQKK IKNPNYQGKW 
       310        320        330        340        350
KAPMIDNPDF KDDPYIYAFD SLKYIGIELW QVKSGTLFDN FLITDDPELA 
       360        370        380        390        400
KTFAEETWGK HKDAEKAAFD EAEKKKEEEE AAKAGEDDDD LDDEDAEDED 
       410        420 
KADEKADSDA EDGKDSDDEK HDEL
Length:424
Mass (Da):48,309
Last modified:December 20, 2005 - v2
Checksum:iEFAE5723CB03BAE0
GO
Isoform 2 (identifier: Q9SLY8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     422-424: DEL → VCNTPAFS

Note: No experimental confirmation available.
Show »
Length:429
Mass (Da):48,772
Checksum:i1BD29FEC132CCBEF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti193T → S in BAA88900 (PubMed:10651810).Curated1
Sequence conflicti263 – 265DWD → AGA in BAA88900 (PubMed:10651810).Curated3
Sequence conflicti278P → R in BAA88900 (PubMed:10651810).Curated1
Sequence conflicti302A → P in BAA88900 (PubMed:10651810).Curated1
Sequence conflicti306 – 307DN → AT in BAA88900 (PubMed:10651810).Curated2
Sequence conflicti311K → Q in BAA88900 (PubMed:10651810).Curated1
Sequence conflicti324Y → S in BAA88900 (PubMed:10651810).Curated1
Sequence conflicti341F → I in BAA88900 (PubMed:10651810).Curated1
Sequence conflicti347 – 348PE → AA in BAA88900 (PubMed:10651810).Curated2
Sequence conflicti359G → A in BAA88900 (PubMed:10651810).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016726422 – 424DEL → VCNTPAFS in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021259 mRNA Translation: BAA88900.1
AP003738 Genomic DNA Translation: BAC82932.1
AP003738 Genomic DNA Translation: BAC82933.1
AP006266 Genomic DNA Translation: BAD31961.1
AP006266 Genomic DNA Translation: BAD31962.1
AP014963 Genomic DNA Translation: BAT00809.1
AP014963 Genomic DNA Translation: BAT00810.1
AK065341 mRNA No translation available.
AK104328 mRNA No translation available.
RefSeqiXP_015647166.1, XM_015791680.1 [Q9SLY8-2]
XP_015647167.1, XM_015791681.1 [Q9SLY8-1]
UniGeneiOs.60530

Genome annotation databases

EnsemblPlantsiOs07t0246200-01; Os07t0246200-01; Os07g0246200 [Q9SLY8-1]
Os07t0246200-02; Os07t0246200-02; Os07g0246200 [Q9SLY8-1]
Os07t0246200-03; Os07t0246200-03; Os07g0246200 [Q9SLY8-2]
GeneIDi4342826
GrameneiOs07t0246200-01; Os07t0246200-01; Os07g0246200 [Q9SLY8-1]
Os07t0246200-02; Os07t0246200-02; Os07g0246200 [Q9SLY8-1]
Os07t0246200-03; Os07t0246200-03; Os07g0246200 [Q9SLY8-2]
KEGGiosa:4342826

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCALR_ORYSJ
AccessioniPrimary (citable) accession number: Q9SLY8
Secondary accession number(s): Q6ZLM7, Q6ZLM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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