Q9SLM6 (GSTF3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase F3
Short name=AtGSTF3
EC=2.5.1.18

Alternative name(s):
GST class-phi member 3
Glutathione S-transferase 16

Gene names

Name:	GSTF3
Synonyms:	GST16
Ordered Locus Names:	At2g02930
ORF Names:	T17M13.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds a series of heterocyclic compounds, including lumichrome, harmane, norharmane and indole-3-aldehyde. May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Probable.
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to fungus Inferred from expression pattern PubMed 21711359. Source: TAIR response to toxin Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.1. Source: TAIR
Cellular_component	cytoplasm Non-traceable author statement Ref.1. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Glutathione S-transferase F3		PRO_0000185853
Regions
	Domain	2 – 83	82	GST N-terminal
	Domain	93 – 212	120	GST C-terminal
	Region	12 – 13	2	Glutathione binding By similarity
	Region	41 – 42	2	Glutathione binding By similarity
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Experimental info
	Sequence conflict	96	1	Q → R in BAF01093. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9SLM6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 73818A85E6D05A59	FASTA	212	24,121
	10 20 30 40 50 60 MAGIKVFGHP ASTSTRRVLI ALHEKNLDFE LVHVELKDGE HKKEPFLSRN PFGQVPAFED 70 80 90 100 110 120 GDLKLFESRA ITQYIAHRYE NQGTNLLPAD SKNIAQYAIM SIGIQVEAHQ FDPVASKLAW 130 140 150 160 170 180 EQVFKFNYGL NTDQAVVAEE EAKLAKVLDV YEARLKEFKY LAGETFTLTD LHHIPVIQYL 190 200 210 LGTPTKKLFT ERPRVNEWVA EITKRPASEK VL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Ecker J.R. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF288181 mRNA. Translation: AAG30130.1. AC004138 Genomic DNA. Translation: AAC32912.1. CP002685 Genomic DNA. Translation: AEC05642.1. BT024921 mRNA. Translation: ABD94077.1. AK229226 mRNA. Translation: BAF01093.1.
IPI	IPI00532945.
PIR	D84442.
RefSeq	NP_178394.1. NM_126346.2.
UniGene	At.22195. At.24972.
3D structure databases
ProteinModelPortal	Q9SLM6.
SMR	Q9SLM6. Positions 3-211.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G02930.1-P.
Proteomic databases
PaxDb	Q9SLM6.
PRIDE	Q9SLM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G02930.1; AT2G02930.1; AT2G02930.
GeneID	814822.
KEGG	ath:AT2G02930.
Organism-specific databases
TAIR	At2g02930.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125746.
InParanoid	Q9SLM6.
KO	K00799.
OMA	SKNIAQY.
PhylomeDB	Q9SLM6.
ProtClustDB	CLSN2679613.
Gene expression databases
ArrayExpress	Q9SLM6.
Genevestigator	Q9SLM6.
GermOnline	AT2G02930. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF3_ARATH

Accession

Primary (citable) accession number: Q9SLM6
Secondary accession number(s): Q0WP56, Q1WWI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program