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Protein

Phospholipid-transporting ATPase 6

Gene

ALA6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transport of phospholipids and in regulation of pollen plasma membrane lipid asymmetry.1 Publication

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei426 – 42614-aspartylphosphate intermediateBy similarity
Metal bindingi888 – 8881MagnesiumBy similarity
Metal bindingi892 – 8921MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • Golgi vesicle budding Source: GO_Central
  • pollen tube growth Source: TAIR
  • regulation of membrane lipid metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G54280-MONOMER.
ReactomeiR-ATH-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase 61 Publication (EC:3.6.3.11 Publication)
Short name:
AtALA61 Publication
Alternative name(s):
Aminophospholipid flippase 61 Publication
Gene namesi
Name:ALA61 Publication
Ordered Locus Names:At1g54280Imported
ORF Names:F20D21.10Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G54280.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7575CytoplasmicSequence analysisAdd
BLAST
Transmembranei76 – 9722HelicalSequence analysisAdd
BLAST
Topological domaini98 – 1014ExtracellularSequence analysis
Transmembranei102 – 12423HelicalSequence analysisAdd
BLAST
Topological domaini125 – 306182CytoplasmicSequence analysisAdd
BLAST
Transmembranei307 – 32822HelicalSequence analysisAdd
BLAST
Topological domaini329 – 36032ExtracellularSequence analysisAdd
BLAST
Transmembranei361 – 37818HelicalSequence analysisAdd
BLAST
Topological domaini379 – 943565CytoplasmicSequence analysisAdd
BLAST
Transmembranei944 – 96320HelicalSequence analysisAdd
BLAST
Topological domaini964 – 97714ExtracellularSequence analysisAdd
BLAST
Transmembranei978 – 99720HelicalSequence analysisAdd
BLAST
Topological domaini998 – 102730CytoplasmicSequence analysisAdd
BLAST
Transmembranei1028 – 105023HelicalSequence analysisAdd
BLAST
Topological domaini1051 – 106313ExtracellularSequence analysisAdd
BLAST
Transmembranei1064 – 108623HelicalSequence analysisAdd
BLAST
Topological domaini1087 – 10926CytoplasmicSequence analysis
Transmembranei1093 – 111321HelicalSequence analysisAdd
BLAST
Topological domaini1114 – 113017ExtracellularSequence analysisAdd
BLAST
Transmembranei1131 – 115525HelicalSequence analysisAdd
BLAST
Topological domaini1156 – 124085CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endomembrane system Source: TAIR
  • Golgi membrane Source: GOC
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • trans-Golgi network Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Ala6 and ala7 double mutants are hypersensitive to temperature stress and are impaired in pollen fitness with an altered lipid composition and short and slow pollen tubes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12401240Phospholipid-transporting ATPase 6PRO_0000046390Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki625 – 625Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki717 – 717Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9SLK6.
PRIDEiQ9SLK6.

Expressioni

Gene expression databases

GenevisibleiQ9SLK6. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G54280.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SLK6.
SMRiQ9SLK6. Positions 611-954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ITKD. Eukaryota.
ENOG410XPYK. LUCA.
HOGENOMiHOG000202528.
InParanoidiQ9SLK6.
KOiK01530.
OMAiPSDVCLQ.
PhylomeDBiQ9SLK6.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PfamiPF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SLK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRRIRSRI RKSHFYTFRC LRPKTLDDQG PHVINGPGYT RIVHCNQPHL
60 70 80 90 100
HLATKLIRYR SNYVSTTRYN LLTFLPKCLY EQFHRVANFY FLVAAILSVF
110 120 130 140 150
PLSPFNKWSM IAPLVFVVGL SMGKEALEDW RRFMQDVEVN SRKASVHKGS
160 170 180 190 200
GDFGRRTWKR IRVGDIVRVE KDEFFPADLL LLSSSYEDGI CYVETMNLDG
210 220 230 240 250
ETNLKVKRCL DATLALEKDE SFQNFSGTIK CEDPNPNLYT FVGNLECDGQ
260 270 280 290 300
VYPLDPNQIL LRDSKLRNTA YVYGVVVFTG HDTKVMQNST KSPSKRSRIE
310 320 330 340 350
KRMDYIIYTL FALLLTVSFI SSLGFAVMTK LLMAEWWYLR PDKPESLTNP
360 370 380 390 400
TNPLYAWVVH LITALLLYGY LIPISLYVSI EVVKVLQAHF INQDLQLYDS
410 420 430 440 450
ESGTPAQART SNLNEELGQV DTILSDKTGT LTCNQMDFLK CSIAGTSYGV
460 470 480 490 500
RASEVELAAA KQMAMDLEEK GEEVANLSMN KGRTQRYAKL ASKTSSDFEL
510 520 530 540 550
ETVVTASDEK DQKQNTGVKG FSFEDNRLMN ENWLNEPNSD DILMFFRILA
560 570 580 590 600
VCHTAIPEVD EDTGMCTYEA ESPDEVAFLV ASREFGFEFT KRTQSSVFIA
610 620 630 640 650
ERFSSSGQPV DREYKILNLL DFTSKRKRMS AIVRDEEGQI LLLCKGADSI
660 670 680 690 700
IFERLSKSGK EYLGATSKHL NVYGEAGLRT LALGYRKLDE TEYAAWNSEF
710 720 730 740 750
HKAKTSVGAD RDEMLEKVSD MMEKELILVG ATAVEDKLQK GVPQCIDNLA
760 770 780 790 800
QAGLKIWVLT GDKMETAINI GYACSLLRQG MKQISISLTN VEESSQNSEA
810 820 830 840 850
AAKESILMQI TNASQMIKIE KDPHAAFALI IDGKTLTYAL KDDVKYQFLA
860 870 880 890 900
LAVDCASVIC CRVSPKQKAL VTRLAKEGTG KTTLAIGDGA NDVGMIQEAD
910 920 930 940 950
IGVGISGVEG MQAVMASDFS IAQFRFLERL LVVHGHWCYK RIAQMICYFF
960 970 980 990 1000
YKNITFGLTL FYFECFTGFS GQSIYNDSYL LLFNVVLTSL PVISLGVFEQ
1010 1020 1030 1040 1050
DVPSDVCLQF PALYQQGPKN LFFDWYRILG WMGNGVYASI VIFTLNLGIF
1060 1070 1080 1090 1100
HVQSFRSDGQ TADMNAMGTA MFTCIIWAVN VQIALTMSHF TWIQHVMIWG
1110 1120 1130 1140 1150
SIGAWYVFLA LYGMLPVKLS GNIFHMLVEI LAPAPIFWLT SLLVIAATTL
1160 1170 1180 1190 1200
PYLFHISYQR SVNPLDHHII QEIKHFRIDV EDERMWKREK SKAREKTKIG
1210 1220 1230 1240
FTARVDAKIR QLRGRLQRKH SVLSVMSGTS SNDTPSSNSQ
Length:1,240
Mass (Da):140,102
Last modified:September 22, 2009 - v2
Checksum:i63866920588AFB9C
GO

Sequence cautioni

The sequence AAD25608.2 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005287 Genomic DNA. Translation: AAD25608.2. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33075.1.
PIRiC96584.
RefSeqiNP_175830.1. NM_104306.2.
UniGeneiAt.52194.

Genome annotation databases

EnsemblPlantsiAT1G54280.1; AT1G54280.1; AT1G54280.
GeneIDi841869.
GrameneiAT1G54280.1; AT1G54280.1; AT1G54280.
KEGGiath:AT1G54280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005287 Genomic DNA. Translation: AAD25608.2. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33075.1.
PIRiC96584.
RefSeqiNP_175830.1. NM_104306.2.
UniGeneiAt.52194.

3D structure databases

ProteinModelPortaliQ9SLK6.
SMRiQ9SLK6. Positions 611-954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G54280.1.

Proteomic databases

PaxDbiQ9SLK6.
PRIDEiQ9SLK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G54280.1; AT1G54280.1; AT1G54280.
GeneIDi841869.
GrameneiAT1G54280.1; AT1G54280.1; AT1G54280.
KEGGiath:AT1G54280.

Organism-specific databases

TAIRiAT1G54280.

Phylogenomic databases

eggNOGiENOG410ITKD. Eukaryota.
ENOG410XPYK. LUCA.
HOGENOMiHOG000202528.
InParanoidiQ9SLK6.
KOiK01530.
OMAiPSDVCLQ.
PhylomeDBiQ9SLK6.

Enzyme and pathway databases

BioCyciARA:AT1G54280-MONOMER.
ReactomeiR-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ9SLK6.

Gene expression databases

GenevisibleiQ9SLK6. AT.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PfamiPF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Inventory of the superfamily of P-type ion pumps in Arabidopsis."
    Axelsen K.B., Palmgren M.G.
    Plant Physiol. 126:696-706(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  4. "Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants."
    Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.
    Mol. Cell. Proteomics 6:601-610(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-625 AND LYS-717.
    Strain: cv. Landsberg erecta.
  5. "Loss of the Arabidopsis thaliana P4-ATPases ALA6 and ALA7 impairs pollen fitness and alters the pollen tube plasma membrane."
    McDowell S.C., Lopez-Marques R.L., Cohen T., Brown E., Rosenberg A., Palmgren M.G., Harper J.F.
    Front. Plant Sci. 6:197-197(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiALA6_ARATH
AccessioniPrimary (citable) accession number: Q9SLK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: September 22, 2009
Last modified: July 6, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.