Reviewed, UniProtKB/Swiss-Prot Q9SLK6 (ALA6_ARATH)

Names and origin

Protein names

Recommended name:
    Phospholipid-transporting ATPase 6
      Short name=AtALA6
    EC=3.6.3.1
Alternative name(s):
    Aminophospholipid flippase 6

Gene names

Name:	ALA6
Ordered Locus Names:	At1g54280
ORF Names:	F20D21.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1240 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in transport of phospholipids Potential.
Catalytic activity	ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IV subfamily.
Sequence caution	The sequence AAD25608.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro phospholipid transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1240	1240	Phospholipid-transporting ATPase 6		PRO_0000046390
Regions
	Topological domain	1 – 75	75	Cytoplasmic Potential
	Transmembrane	76 – 97	22	Potential
	Topological domain	98 – 101	4	Extracellular Potential
	Transmembrane	102 – 124	23	Potential
	Topological domain	125 – 306	182	Cytoplasmic Potential
	Transmembrane	307 – 328	22	Potential
	Topological domain	329 – 360	32	Extracellular Potential
	Transmembrane	361 – 378	18	Potential
	Topological domain	379 – 943	565	Cytoplasmic Potential
	Transmembrane	944 – 963	20	Potential
	Topological domain	964 – 977	14	Extracellular Potential
	Transmembrane	978 – 997	20	Potential
	Topological domain	998 – 1027	30	Cytoplasmic Potential
	Transmembrane	1028 – 1050	23	Potential
	Topological domain	1051 – 1063	13	Extracellular Potential
	Transmembrane	1064 – 1086	23	Potential
	Topological domain	1087 – 1092	6	Cytoplasmic Potential
	Transmembrane	1093 – 1113	21	Potential
	Topological domain	1114 – 1130	17	Extracellular Potential
	Transmembrane	1131 – 1155	25	Potential
	Topological domain	1156 – 1240	85	Cytoplasmic Potential
Sites
	Active site	426	1	4-aspartylphosphate intermediate By similarity
	Metal binding	888	1	Magnesium By similarity
	Metal binding	892	1	Magnesium By similarity
Amino acid modifications
	Cross-link	625		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.2
	Cross-link	717		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9SLK6-1 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 63866920588AFB9C	FASTA	1,240	140,102
	10 20 30 40 50 60 MARRRIRSRI RKSHFYTFRC LRPKTLDDQG PHVINGPGYT RIVHCNQPHL HLATKLIRYR 70 80 90 100 110 120 SNYVSTTRYN LLTFLPKCLY EQFHRVANFY FLVAAILSVF PLSPFNKWSM IAPLVFVVGL 130 140 150 160 170 180 SMGKEALEDW RRFMQDVEVN SRKASVHKGS GDFGRRTWKR IRVGDIVRVE KDEFFPADLL 190 200 210 220 230 240 LLSSSYEDGI CYVETMNLDG ETNLKVKRCL DATLALEKDE SFQNFSGTIK CEDPNPNLYT 250 260 270 280 290 300 FVGNLECDGQ VYPLDPNQIL LRDSKLRNTA YVYGVVVFTG HDTKVMQNST KSPSKRSRIE 310 320 330 340 350 360 KRMDYIIYTL FALLLTVSFI SSLGFAVMTK LLMAEWWYLR PDKPESLTNP TNPLYAWVVH 370 380 390 400 410 420 LITALLLYGY LIPISLYVSI EVVKVLQAHF INQDLQLYDS ESGTPAQART SNLNEELGQV 430 440 450 460 470 480 DTILSDKTGT LTCNQMDFLK CSIAGTSYGV RASEVELAAA KQMAMDLEEK GEEVANLSMN 490 500 510 520 530 540 KGRTQRYAKL ASKTSSDFEL ETVVTASDEK DQKQNTGVKG FSFEDNRLMN ENWLNEPNSD 550 560 570 580 590 600 DILMFFRILA VCHTAIPEVD EDTGMCTYEA ESPDEVAFLV ASREFGFEFT KRTQSSVFIA 610 620 630 640 650 660 ERFSSSGQPV DREYKILNLL DFTSKRKRMS AIVRDEEGQI LLLCKGADSI IFERLSKSGK 670 680 690 700 710 720 EYLGATSKHL NVYGEAGLRT LALGYRKLDE TEYAAWNSEF HKAKTSVGAD RDEMLEKVSD 730 740 750 760 770 780 MMEKELILVG ATAVEDKLQK GVPQCIDNLA QAGLKIWVLT GDKMETAINI GYACSLLRQG 790 800 810 820 830 840 MKQISISLTN VEESSQNSEA AAKESILMQI TNASQMIKIE KDPHAAFALI IDGKTLTYAL 850 860 870 880 890 900 KDDVKYQFLA LAVDCASVIC CRVSPKQKAL VTRLAKEGTG KTTLAIGDGA NDVGMIQEAD 910 920 930 940 950 960 IGVGISGVEG MQAVMASDFS IAQFRFLERL LVVHGHWCYK RIAQMICYFF YKNITFGLTL 970 980 990 1000 1010 1020 FYFECFTGFS GQSIYNDSYL LLFNVVLTSL PVISLGVFEQ DVPSDVCLQF PALYQQGPKN 1030 1040 1050 1060 1070 1080 LFFDWYRILG WMGNGVYASI VIFTLNLGIF HVQSFRSDGQ TADMNAMGTA MFTCIIWAVN 1090 1100 1110 1120 1130 1140 VQIALTMSHF TWIQHVMIWG SIGAWYVFLA LYGMLPVKLS GNIFHMLVEI LAPAPIFWLT 1150 1160 1170 1180 1190 1200 SLLVIAATTL PYLFHISYQR SVNPLDHHII QEIKHFRIDV EDERMWKREK SKAREKTKIG 1210 1220 1230 1240 FTARVDAKIR QLRGRLQRKH SVLSVMSGTS SNDTPSSNSQ

References

[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants." Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K. Mol. Cell. Proteomics 6:601-610(2007) [PubMed: 17272265] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-625 AND LYS-717, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005287 Genomic DNA. Translation: AAD25608.2. Sequence problems.
IPI	IPI00546745.
PIR	C96584.
RefSeq	NP_175830.1.
UniGene	At.52194 Rsa.20384
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SLK6.
Proteomic databases
PRIDE	Q9SLK6.
Genome annotation databases
GeneID	841869.
GenomeReviews	Gene locus AT1G54280 in contig CT485782_GR.
KEGG	ath:AT1G54280.
Organism-specific databases
TAIR	At1g54280.
Phylogenomic databases
eggNOG	KOG0206.
HOGENOM	HBG745019.
InParanoid	Q9SLK6.
PhylomeDB	Q9SLK6.
Enzyme and pathway databases
BRENDA	3.6.3.1. 302.
Gene expression databases
ArrayExpress	Q9SLK6.
Genevestigator	Q9SLK6.
GermOnline	AT1G54280. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 3 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALA6_ARATH

Accession

Primary (citable) accession number: Q9SLK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	September 22, 2009
Last modified:	February 9, 2010
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)