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Protein

Peroxisomal isocitrate dehydrogenase [NADP]

Gene

ICDH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in response to oxidative stresses.By similarity

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79SubstrateBy similarity1
Binding sitei84NADPBy similarity1
Binding sitei111SubstrateBy similarity1
Binding sitei134SubstrateBy similarity1
Sitei141Critical for catalysisBy similarity1
Sitei213Critical for catalysisBy similarity1
Metal bindingi253Magnesium or manganeseBy similarity1
Binding sitei261NADPBy similarity1
Metal bindingi276Magnesium or manganeseBy similarity1
Binding sitei329NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 79NADPBy similarity3
Nucleotide bindingi311 – 316NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT1G54340-MONOMER.
ReactomeiR-ATH-389542. NADPH regeneration.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-71403. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Gene namesi
Name:ICDH
Ordered Locus Names:At1g54340
ORF Names:F20D21.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G54340.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004219621 – 416Peroxisomal isocitrate dehydrogenase [NADP]Add BLAST416

Proteomic databases

PaxDbiQ9SLK0.
PRIDEiQ9SLK0.

Expressioni

Gene expression databases

GenevisibleiQ9SLK0. AT.

Interactioni

Protein-protein interaction databases

BioGridi27100. 1 interactor.
STRINGi3702.AT1G54340.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SLK0.
SMRiQ9SLK0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 102Substrate bindingBy similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi414 – 416Peroxisomal targeting signalCurated3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
HOGENOMiHOG000019858.
InParanoidiQ9SLK0.
KOiK00031.
OMAiPHWEKPI.
OrthoDBiEOG09360AQU.
PhylomeDBiQ9SLK0.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SLK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFEKIKVIN PVVEMDGDEM TRVIWKFIKD KLIFPFLELD IKYFDLGLPN
60 70 80 90 100
RDFTDDKVTI ETAEATLKYN VAIKCATITP DEARVREFGL KKMWRSPNGT
110 120 130 140 150
IRNILNGTVF REPIICRNIP RLVPGWTKPI CIGRHAFGDQ YRATDLIVNE
160 170 180 190 200
PGKLKLVFEP SGSSQKTEFE VFNFTGGGVA LAMYNTDESI RAFAESSMYT
210 220 230 240 250
AYQKKWPLYL STKNTILKIY DGRFKDIFQE VYEANWRSKY EAAGIWYEHR
260 270 280 290 300
LIDDMVAYAM KSEGGYVWAC KNYDGDVQSD FLAQGYGSLG MMTSVLVCPD
310 320 330 340 350
GKTIEAEAAH GTVTRHYRVH QKGGETSTNS IASIFAWSRG LAHRAKLDSN
360 370 380 390 400
AALLSYTEKL EAACMGTVES GKMTKDLALL IHGAKVRRDQ YVNTEEFIDA
410
VAWELKRRLL GNNSRL
Length:416
Mass (Da):47,234
Last modified:May 1, 2000 - v1
Checksum:iEEAAAC9E5C6B2FA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316501 mRNA. Translation: AAK06592.1.
AC005287 Genomic DNA. Translation: AAD25614.1.
CP002684 Genomic DNA. Translation: AEE33082.1.
BT025983 mRNA. Translation: ABG25072.1.
PIRiA96585.
RefSeqiNP_175836.1. NM_104312.3.
UniGeneiAt.11811.
At.37230.

Genome annotation databases

EnsemblPlantsiAT1G54340.1; AT1G54340.1; AT1G54340.
GeneIDi841875.
GrameneiAT1G54340.1; AT1G54340.1; AT1G54340.
KEGGiath:AT1G54340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316501 mRNA. Translation: AAK06592.1.
AC005287 Genomic DNA. Translation: AAD25614.1.
CP002684 Genomic DNA. Translation: AEE33082.1.
BT025983 mRNA. Translation: ABG25072.1.
PIRiA96585.
RefSeqiNP_175836.1. NM_104312.3.
UniGeneiAt.11811.
At.37230.

3D structure databases

ProteinModelPortaliQ9SLK0.
SMRiQ9SLK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27100. 1 interactor.
STRINGi3702.AT1G54340.1.

Proteomic databases

PaxDbiQ9SLK0.
PRIDEiQ9SLK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G54340.1; AT1G54340.1; AT1G54340.
GeneIDi841875.
GrameneiAT1G54340.1; AT1G54340.1; AT1G54340.
KEGGiath:AT1G54340.

Organism-specific databases

TAIRiAT1G54340.

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
HOGENOMiHOG000019858.
InParanoidiQ9SLK0.
KOiK00031.
OMAiPHWEKPI.
OrthoDBiEOG09360AQU.
PhylomeDBiQ9SLK0.

Enzyme and pathway databases

BioCyciARA:AT1G54340-MONOMER.
ReactomeiR-ATH-389542. NADPH regeneration.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiQ9SLK0.

Gene expression databases

GenevisibleiQ9SLK0. AT.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICDHX_ARATH
AccessioniPrimary (citable) accession number: Q9SLK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.