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Protein

DELLA protein RGA

Gene

RGA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression in seeds. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Compared to other DELLA proteins, it is the most sensitive to GA application. No effect of the BOI proteins on its stability. Its activity is probably regulated by other phytohormones such as auxin and ethylene, attenuation of auxin transport delaying its GA-induced degradation.10 Publications

GO - Molecular functioni

  • DNA binding transcription factor activity Source: TAIR
  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, transcription factor binding Source: InterPro
  • transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

  • gibberellic acid mediated signaling pathway Source: TAIR
  • meiotic cytokinesis Source: TAIR
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of developmental vegetative growth Source: CAFA
  • negative regulation of gibberellic acid mediated signaling pathway Source: CAFA
  • negative regulation of leaf development Source: CAFA
  • negative regulation of trichome patterning Source: CAFA
  • positive regulation of gene expression Source: CAFA
  • regulation of protein catabolic process Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • response to far red light Source: TAIR
  • response to gibberellin Source: CAFA
  • salicylic acid mediated signaling pathway Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processGibberellin signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DELLA protein RGA
Alternative name(s):
GAI-related sequence
GRAS family protein 10
Short name:
AtGRAS-10
Repressor on the ga1-3 mutant
Restoration of growth on ammonia protein 1
Gene namesi
Name:RGA
Synonyms:GRS, RGA1
Ordered Locus Names:At2g01570
ORF Names:F2I9.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT2G01570
TAIRilocus:2005516 AT2G01570

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Rga, gai, rgl1, rgl2 and rgl3 pentuple mutant displays constitutive GA responses even in the absence of GA treatment.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44 – 60Missing in rga-delta17; induces resistance to GA-induced degradation but does not affect nuclear localization. 1 PublicationAdd BLAST17
Mutagenesisi341Q → R: Causes a semidwarf phenotype by abolishing the interaction with GID2 leading to prevent its degradation. 1 Publication1
Mutagenesisi478D → N in rga-2; partially suppresses phenotypic defects of GA-mutant ga1-3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001322341 – 587DELLA protein RGAAdd BLAST587

Post-translational modificationi

Phosphorylated. Phosphorylation may increase the interaction with GID2.1 Publication
Gibberellin (GA) induces dephosphorylation of RGA by TOPP4 and subsequent degradation by the proteasomal pathway.1 Publication
Ubiquitinated. Upon GA application it is ubiquitinated by the SCF(GID2) complex, leading to its subsequent degradation.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9SLH3

PTM databases

iPTMnetiQ9SLH3

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in roots, rosette leaves, bolting and mature stems, young and mature siliques, flower buds and influorescences.4 Publications

Gene expression databases

ExpressionAtlasiQ9SLH3 baseline and differential
GenevisibleiQ9SLH3 AT

Interactioni

Subunit structurei

Interacts directly with the GID2/SLY1 component of the SCF(GID2) complex. Interacts (via N-terminus) with GID1A, GID1B and GID1B (via N-terminus). Binds to bHLH transcription factors such as MYC2, PIF1, PIF4, PIF6 and SPT. Interacts with the BOI proteins BOI, BRG1, BRG2 and BRG3. Interacts with NFYC9 (PubMed:25105952). Interacts with TOPP4 (PubMed:25010794).9 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi89, 67 interactors
DIPiDIP-32983N
IntActiQ9SLH3, 24 interactors
MINTiQ9SLH3
STRINGi3702.AT2G01570.1

Structurei

3D structure databases

ProteinModelPortaliQ9SLH3
SMRiQ9SLH3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini212 – 581GRASPROSITE-ProRule annotationAdd BLAST370

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 273Leucine repeat I (LRI)PROSITE-ProRule annotationAdd BLAST55
Regioni292 – 357VHIIDPROSITE-ProRule annotationAdd BLAST66
Regioni371 – 403Leucine repeat II (LRII)PROSITE-ProRule annotationAdd BLAST33
Regioni415 – 502PFYREPROSITE-ProRule annotationAdd BLAST88
Regioni505 – 581SAWPROSITE-ProRule annotationAdd BLAST77

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi44 – 48DELLA motif5
Motifi66 – 70LEXLE motif5
Motifi89 – 93VHYNP motif5
Motifi323 – 327VHIIDPROSITE-ProRule annotation5
Motifi423 – 427LXXLL motifPROSITE-ProRule annotation5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi190 – 200Poly-ThrAdd BLAST11

Domaini

The DELLA motif is required for its GA-induced degradation but not for the interaction with GID2.1 Publication

Sequence similaritiesi

Belongs to the GRAS family. DELLA subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IQYH Eukaryota
ENOG410YBFY LUCA
HOGENOMiHOG000238577
InParanoidiQ9SLH3
KOiK14494
OMAiPPQNQID
OrthoDBiEOG0936075R
PhylomeDBiQ9SLH3

Family and domain databases

Gene3Di1.10.10.1290, 1 hit
InterProiView protein in InterPro
IPR038088 DELLA_N_sf
IPR030006 TF_DELLA
IPR021914 TF_DELLA_N
IPR005202 TF_GRAS
PANTHERiPTHR31636:SF47 PTHR31636:SF47, 1 hit
PfamiView protein in Pfam
PF12041 DELLA, 1 hit
PF03514 GRAS, 1 hit
PROSITEiView protein in PROSITE
PS50985 GRAS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SLH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRDHHQFQG RLSNHGTSSS SSSISKDKMM MVKKEEDGGG NMDDELLAVL
60 70 80 90 100
GYKVRSSEMA EVALKLEQLE TMMSNVQEDG LSHLATDTVH YNPSELYSWL
110 120 130 140 150
DNMLSELNPP PLPASSNGLD PVLPSPEICG FPASDYDLKV IPGNAIYQFP
160 170 180 190 200
AIDSSSSSNN QNKRLKSCSS PDSMVTSTST GTQIGGVIGT TVTTTTTTTT
210 220 230 240 250
AAGESTRSVI LVDSQENGVR LVHALMACAE AIQQNNLTLA EALVKQIGCL
260 270 280 290 300
AVSQAGAMRK VATYFAEALA RRIYRLSPPQ NQIDHCLSDT LQMHFYETCP
310 320 330 340 350
YLKFAHFTAN QAILEAFEGK KRVHVIDFSM NQGLQWPALM QALALREGGP
360 370 380 390 400
PTFRLTGIGP PAPDNSDHLH EVGCKLAQLA EAIHVEFEYR GFVANSLADL
410 420 430 440 450
DASMLELRPS DTEAVAVNSV FELHKLLGRP GGIEKVLGVV KQIKPVIFTV
460 470 480 490 500
VEQESNHNGP VFLDRFTESL HYYSTLFDSL EGVPNSQDKV MSEVYLGKQI
510 520 530 540 550
CNLVACEGPD RVERHETLSQ WGNRFGSSGL APAHLGSNAF KQASMLLSVF
560 570 580
NSGQGYRVEE SNGCLMLGWH TRPLITTSAW KLSTAAY
Length:587
Mass (Da):64,035
Last modified:May 1, 2000 - v1
Checksum:iFC92E7F9408072AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86T → A in CAA75493 (PubMed:9389651).Curated1
Sequence conflicti139K → E in CAA75493 (PubMed:9389651).Curated1
Sequence conflicti203G → A in CAA72177 (PubMed:9237632).Curated1
Sequence conflicti203G → A in CAA75493 (PubMed:9389651).Curated1
Sequence conflicti209V → D in AAK97709 (PubMed:14593172).Curated1
Sequence conflicti383I → V in CAA75493 (PubMed:9389651).Curated1
Sequence conflicti587Y → H in CAA72177 (PubMed:9237632).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11336 mRNA Translation: CAA72177.1
Y15194 mRNA Translation: CAA75493.1
AC005560 Genomic DNA Translation: AAC67333.1
CP002685 Genomic DNA Translation: AEC05469.1
AY052239 mRNA Translation: AAK97709.1
AY054160 mRNA Translation: AAL06821.1
BT010467 mRNA Translation: AAQ65090.1
PIRiD84426
RefSeqiNP_178266.1, NM_126218.3
UniGeneiAt.24012

Genome annotation databases

EnsemblPlantsiAT2G01570.1; AT2G01570.1; AT2G01570
GeneIDi814686
GrameneiAT2G01570.1; AT2G01570.1; AT2G01570
KEGGiath:AT2G01570

Similar proteinsi

Entry informationi

Entry nameiRGA_ARATH
AccessioniPrimary (citable) accession number: Q9SLH3
Secondary accession number(s): O23642, O23725, Q941D4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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