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Protein

DELLA protein RGA

Gene

RGA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression in seeds. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Compared to other DELLA proteins, it is the most sensitive to GA application. No effect of the BOI proteins on its stability. Its activity is probably regulated by other phytohormones such as auxin and ethylene, attenuation of auxin transport delaying its GA-induced degradation.10 Publications

GO - Molecular functioni

GO - Biological processi

  • gibberellic acid mediated signaling pathway Source: TAIR
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of gibberellic acid mediated signaling pathway Source: TAIR
  • regulation of protein catabolic process Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • response to far red light Source: TAIR
  • salicylic acid mediated signaling pathway Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Gibberellin signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DELLA protein RGA
Alternative name(s):
GAI-related sequence
GRAS family protein 10
Short name:
AtGRAS-10
Repressor on the ga1-3 mutant
Restoration of growth on ammonia protein 1
Gene namesi
Name:RGA
Synonyms:GRS, RGA1
Ordered Locus Names:At2g01570
ORF Names:F2I9.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01570.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Rga, gai, rgl1, rgl2 and rgl3 pentuple mutant displays constitutive GA responses even in the absence of GA treatment.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi44 – 6017Missing in rga-delta17; induces resistance to GA-induced degradation but does not affect nuclear localization. 1 PublicationAdd
BLAST
Mutagenesisi341 – 3411Q → R: Causes a semidwarf phenotype by abolishing the interaction with GID2 leading to prevent its degradation. 1 Publication
Mutagenesisi478 – 4781D → N in rga-2; partially suppresses phenotypic defects of GA-mutant ga1-3. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 587587DELLA protein RGAPRO_0000132234Add
BLAST

Post-translational modificationi

Phosphorylated. Phosphorylation may increase the interaction with GID2.1 Publication
Ubiquitinated. Upon GA application it is ubiquitinated by the SCF(GID2) complex, leading to its subsequent degradation.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9SLH3.
PRIDEiQ9SLH3.

PTM databases

iPTMnetiQ9SLH3.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in roots, rosette leaves, bolting and mature stems, young and mature siliques, flower buds and influorescences.4 Publications

Gene expression databases

GenevisibleiQ9SLH3. AT.

Interactioni

Subunit structurei

Interacts directly with the GID2/SLY1 component of the SCF(GID2) complex. Interacts (via N-terminus) with GID1A, GID1B and GID1B (via N-terminus). Binds to bHLH transcription factors such as MYC2, PIF1, PIF4, PIF6 and SPT. Interacts with the BOI proteins BOI, BRG1, BRG2 and BRG3.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
COQ390573EBI-963624,EBI-1639724
GID1AQ9MAA77EBI-963624,EBI-963597
GID1BQ9LYC16EBI-963624,EBI-963686
GID1CQ940G65EBI-963624,EBI-963794
GID2Q9STX34EBI-963624,EBI-619033

Protein-protein interaction databases

BioGridi89. 67 interactions.
DIPiDIP-32983N.
IntActiQ9SLH3. 14 interactions.
MINTiMINT-8068545.
STRINGi3702.AT2G01570.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SLH3.
SMRiQ9SLH3. Positions 44-108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi44 – 485DELLA motif
Motifi66 – 705LEXLE motif
Motifi89 – 935VHYNP motif
Motifi323 – 3275VHIID
Motifi423 – 4275LXXLL motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi190 – 20011Poly-ThrAdd
BLAST

Domaini

The DELLA motif is required for its GA-induced degradation but not for the interaction with GID2.1 Publication

Sequence similaritiesi

Belongs to the GRAS family. DELLA subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IQYH. Eukaryota.
ENOG410YBFY. LUCA.
HOGENOMiHOG000238577.
InParanoidiQ9SLH3.
KOiK14494.
OMAiDKMMMVK.
PhylomeDBiQ9SLH3.

Family and domain databases

InterProiIPR030006. TF_DELLA.
IPR021914. TF_DELLA_N.
IPR005202. TF_GRAS.
[Graphical view]
PANTHERiPTHR31636:SF50. PTHR31636:SF50. 1 hit.
PfamiPF12041. DELLA. 1 hit.
PF03514. GRAS. 1 hit.
[Graphical view]
PROSITEiPS50985. GRAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SLH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRDHHQFQG RLSNHGTSSS SSSISKDKMM MVKKEEDGGG NMDDELLAVL
60 70 80 90 100
GYKVRSSEMA EVALKLEQLE TMMSNVQEDG LSHLATDTVH YNPSELYSWL
110 120 130 140 150
DNMLSELNPP PLPASSNGLD PVLPSPEICG FPASDYDLKV IPGNAIYQFP
160 170 180 190 200
AIDSSSSSNN QNKRLKSCSS PDSMVTSTST GTQIGGVIGT TVTTTTTTTT
210 220 230 240 250
AAGESTRSVI LVDSQENGVR LVHALMACAE AIQQNNLTLA EALVKQIGCL
260 270 280 290 300
AVSQAGAMRK VATYFAEALA RRIYRLSPPQ NQIDHCLSDT LQMHFYETCP
310 320 330 340 350
YLKFAHFTAN QAILEAFEGK KRVHVIDFSM NQGLQWPALM QALALREGGP
360 370 380 390 400
PTFRLTGIGP PAPDNSDHLH EVGCKLAQLA EAIHVEFEYR GFVANSLADL
410 420 430 440 450
DASMLELRPS DTEAVAVNSV FELHKLLGRP GGIEKVLGVV KQIKPVIFTV
460 470 480 490 500
VEQESNHNGP VFLDRFTESL HYYSTLFDSL EGVPNSQDKV MSEVYLGKQI
510 520 530 540 550
CNLVACEGPD RVERHETLSQ WGNRFGSSGL APAHLGSNAF KQASMLLSVF
560 570 580
NSGQGYRVEE SNGCLMLGWH TRPLITTSAW KLSTAAY
Length:587
Mass (Da):64,035
Last modified:May 1, 2000 - v1
Checksum:iFC92E7F9408072AA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861T → A in CAA75493 (PubMed:9389651).Curated
Sequence conflicti139 – 1391K → E in CAA75493 (PubMed:9389651).Curated
Sequence conflicti203 – 2031G → A in CAA72177 (PubMed:9237632).Curated
Sequence conflicti203 – 2031G → A in CAA75493 (PubMed:9389651).Curated
Sequence conflicti209 – 2091V → D in AAK97709 (PubMed:14593172).Curated
Sequence conflicti383 – 3831I → V in CAA75493 (PubMed:9389651).Curated
Sequence conflicti587 – 5871Y → H in CAA72177 (PubMed:9237632).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11336 mRNA. Translation: CAA72177.1.
Y15194 mRNA. Translation: CAA75493.1.
AC005560 Genomic DNA. Translation: AAC67333.1.
CP002685 Genomic DNA. Translation: AEC05469.1.
AY052239 mRNA. Translation: AAK97709.1.
AY054160 mRNA. Translation: AAL06821.1.
BT010467 mRNA. Translation: AAQ65090.1.
PIRiD84426.
RefSeqiNP_178266.1. NM_126218.2.
UniGeneiAt.24012.

Genome annotation databases

EnsemblPlantsiAT2G01570.1; AT2G01570.1; AT2G01570.
GeneIDi814686.
GrameneiAT2G01570.1; AT2G01570.1; AT2G01570.
KEGGiath:AT2G01570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11336 mRNA. Translation: CAA72177.1.
Y15194 mRNA. Translation: CAA75493.1.
AC005560 Genomic DNA. Translation: AAC67333.1.
CP002685 Genomic DNA. Translation: AEC05469.1.
AY052239 mRNA. Translation: AAK97709.1.
AY054160 mRNA. Translation: AAL06821.1.
BT010467 mRNA. Translation: AAQ65090.1.
PIRiD84426.
RefSeqiNP_178266.1. NM_126218.2.
UniGeneiAt.24012.

3D structure databases

ProteinModelPortaliQ9SLH3.
SMRiQ9SLH3. Positions 44-108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi89. 67 interactions.
DIPiDIP-32983N.
IntActiQ9SLH3. 14 interactions.
MINTiMINT-8068545.
STRINGi3702.AT2G01570.1.

PTM databases

iPTMnetiQ9SLH3.

Proteomic databases

PaxDbiQ9SLH3.
PRIDEiQ9SLH3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01570.1; AT2G01570.1; AT2G01570.
GeneIDi814686.
GrameneiAT2G01570.1; AT2G01570.1; AT2G01570.
KEGGiath:AT2G01570.

Organism-specific databases

TAIRiAT2G01570.

Phylogenomic databases

eggNOGiENOG410IQYH. Eukaryota.
ENOG410YBFY. LUCA.
HOGENOMiHOG000238577.
InParanoidiQ9SLH3.
KOiK14494.
OMAiDKMMMVK.
PhylomeDBiQ9SLH3.

Miscellaneous databases

PROiQ9SLH3.

Gene expression databases

GenevisibleiQ9SLH3. AT.

Family and domain databases

InterProiIPR030006. TF_DELLA.
IPR021914. TF_DELLA_N.
IPR005202. TF_GRAS.
[Graphical view]
PANTHERiPTHR31636:SF50. PTHR31636:SF50. 1 hit.
PfamiPF12041. DELLA. 1 hit.
PF03514. GRAS. 1 hit.
[Graphical view]
PROSITEiPS50985. GRAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and characterization of two Arabidopsis thaliana cDNAs isolated by functional complementation of a yeast gln3 gdh1 mutant."
    Truong H.-N., Caboche M., Daniel-Vedele F.
    FEBS Lett. 410:213-218(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses."
    Peng J., Carol P., Richards D.E., King K.E., Cowling R.J., Murphy G.P., Harberd N.P.
    Genes Dev. 11:3194-3205(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway."
    Silverstone A.L., Ciampaglio C.N., Sun T.-P.
    Plant Cell 10:155-169(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-478.
  7. "Gibberellins are not required for normal stem growth in Arabidopsis thaliana in the absence of GAI and RGA."
    King K.E., Moritz T., Harberd N.P.
    Genetics 159:767-776(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana."
    Dill A., Sun T.-P.
    Genetics 159:777-785(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The DELLA motif is essential for gibberellin-induced degradation of RGA."
    Dill A., Jung H.-S., Sun T.-P.
    Proc. Natl. Acad. Sci. U.S.A. 98:14162-14167(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, MUTAGENESIS OF 44-ASP--ALA-60.
  10. "Gibberellin regulates Arabidopsis seed germination via RGL2, a GAI/RGA-like gene whose expression is up-regulated following imbibition."
    Lee S., Cheng H., King K.E., Wang W., He Y., Hussain A., Lo J., Harberd N.P., Peng J.
    Genes Dev. 16:646-658(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  11. "Auxin promotes Arabidopsis root growth by modulating gibberellin response."
    Fu X., Harberd N.P.
    Nature 421:740-743(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "The Arabidopsis SLEEPY1 gene encodes a putative F-box subunit of an SCF E3 ubiquitin ligase."
    McGinnis K.M., Thomas S.G., Soule J.D., Strader L.C., Zale J.M., Sun T.-P., Steber C.M.
    Plant Cell 15:1120-1130(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEGRADATION.
  13. "Ethylene regulates Arabidopsis development via the modulation of DELLA protein growth repressor function."
    Achard P., Vriezen W.H., Van Der Straeten D., Harberd N.P.
    Plant Cell 15:2816-2825(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function."
    Cheng H., Qin L., Lee S., Fu X., Richards D.E., Cao D., Luo D., Harberd N.P., Peng J.
    Development 131:1055-1064(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "The Arabidopsis F-box protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation."
    Dill A., Thomas S.G., Hu J., Steber C.M., Sun T.-P.
    Plant Cell 16:1392-1405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE UBIQUITINATION, INTERACTION WITH GID2.
  16. "The Arabidopsis mutant sleepy1gar2-1 protein promotes plant growth by increasing the affinity of the SCFSLY1 E3 ubiquitin ligase for DELLA protein substrates."
    Fu X., Richards D.E., Fleck B., Xie D., Burton N., Harberd N.P.
    Plant Cell 16:1406-1418(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GID2, PHOSPHORYLATION.
  17. "Della proteins and gibberellin-regulated seed germination and floral development in Arabidopsis."
    Tyler L., Thomas S.G., Hu J., Dill A., Alonso J.M., Ecker J.R., Sun T.-P.
    Plant Physiol. 135:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GID2.
  18. "Floral homeotic genes are targets of gibberellin signaling in flower development."
    Yu H., Ito T., Zhao Y., Peng J., Kumar P., Meyerowitz E.M.
    Proc. Natl. Acad. Sci. U.S.A. 101:7827-7832(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  19. "Loss of function of four DELLA genes leads to light- and gibberellin-independent seed germination in Arabidopsis."
    Cao D., Hussain A., Cheng H., Peng J.
    Planta 223:105-113(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  20. "A novel dwarfing mutation in a green revolution gene from Brassica rapa."
    Muangprom A., Thomas S.-G., Sun T.-P., Osborn T.C.
    Plant Physiol. 137:931-938(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLN-341.
  21. Cited for: INTERACTION WITH GID1A; GID1B AND GID1C.
  22. "Global analysis of della direct targets in early gibberellin signaling in Arabidopsis."
    Zentella R., Zhang Z.L., Park M., Thomas S.G., Endo A., Murase K., Fleet C.M., Jikumaru Y., Nambara E., Kamiya Y., Sun T.P.
    Plant Cell 19:3037-3057(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  23. "Transcriptional diversification and functional conservation between DELLA proteins in Arabidopsis."
    Gallego-Bartolome J., Minguet E.G., Marin J.A., Prat S., Blazquez M.A., Alabadi D.
    Mol. Biol. Evol. 27:1247-1256(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH PIF1; PIF4; PIF6 AND SPT.
    Strain: cv. Landsberg erecta.
  24. "Arabidopsis MYC2 interacts with DELLA proteins in regulating sesquiterpene synthase gene expression."
    Hong G.J., Xue X.Y., Mao Y.B., Wang L.J., Chen X.Y.
    Plant Cell 24:2635-2648(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MYC2.
  25. "DELLA proteins and their interacting RING Finger proteins repress gibberellin responses by binding to the promoters of a subset of gibberellin-responsive genes in Arabidopsis."
    Park J., Nguyen K.T., Park E., Jeon J.S., Choi G.
    Plant Cell 25:927-943(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BOI; BRG1; BRG2 AND BRG3, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiRGA_ARATH
AccessioniPrimary (citable) accession number: Q9SLH3
Secondary accession number(s): O23642, O23725, Q941D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.