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Protein

Nuclear transcription factor Y subunit B-1

Gene

NFYB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the NF-Y/HAP transcription factor complex. The NF-Y complex stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi26 – 327By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-2426168. Activation of gene expression by SREBF (SREBP).

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transcription factor Y subunit B-1
Short name:
AtNF-YB-1
Alternative name(s):
Transcriptional activator HAP3A
Gene namesi
Name:NFYB1
Synonyms:HAP3A
Ordered Locus Names:At2g38880
ORF Names:T7F6.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G38880.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 141140Nuclear transcription factor Y subunit B-1PRO_0000204615Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ9SLG0.

PTM databases

iPTMnetiQ9SLG0.

Expressioni

Tissue specificityi

Ubiquitous. Predominantly expressed in leaves, flowers and siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ9SLG0. baseline and differential.
GenevisibleiQ9SLG0. AT.

Interactioni

Subunit structurei

Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
COQ390576EBI-2126009,EBI-1639724

Protein-protein interaction databases

BioGridi3812. 15 interactions.
IntActiQ9SLG0. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9SLG0.
SMRiQ9SLG0. Positions 20-110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 6412Subunit association domain (SAD)By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the NFYB/HAP3 subunit family.Curated

Phylogenomic databases

HOGENOMiHOG000211832.
InParanoidiQ9SLG0.
PhylomeDBiQ9SLG0.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
IPR003956. Transcrpt_fac_NFYB/HAP3_CS.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00685. NFYB_HAP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SLG0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADTPSSPAG DGGESGGSVR EQDRYLPIAN ISRIMKKALP PNGKIGKDAK
60 70 80 90 100
DTVQECVSEF ISFITSEASD KCQKEKRKTV NGDDLLWAMA TLGFEDYLEP
110 120 130 140
LKIYLARYRE LEGDNKGSGK SGDGSNRDAG GGVSGEEMPS W
Length:141
Mass (Da):15,181
Last modified:June 1, 2002 - v2
Checksum:iD33060B5AD21D9E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13723 mRNA. Translation: CAA74051.1.
AC005770 Genomic DNA. Translation: AAC79602.2.
CP002685 Genomic DNA. Translation: AEC09598.1.
CP002685 Genomic DNA. Translation: AEC09600.1.
CP002685 Genomic DNA. Translation: AEC09602.1.
BT004266 mRNA. Translation: AAO42268.1.
BT005536 mRNA. Translation: AAO63956.1.
AY088554 mRNA. Translation: AAM66086.1.
PIRiE84810.
RefSeqiNP_001031511.1. NM_001036434.1. [Q9SLG0-1]
NP_030436.1. NM_129445.2. [Q9SLG0-1]
NP_850304.2. NM_179973.3. [Q9SLG0-1]
UniGeneiAt.24820.

Genome annotation databases

EnsemblPlantsiAT2G38880.1; AT2G38880.1; AT2G38880. [Q9SLG0-1]
AT2G38880.2; AT2G38880.2; AT2G38880. [Q9SLG0-1]
AT2G38880.5; AT2G38880.5; AT2G38880. [Q9SLG0-1]
GeneIDi818472.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13723 mRNA. Translation: CAA74051.1.
AC005770 Genomic DNA. Translation: AAC79602.2.
CP002685 Genomic DNA. Translation: AEC09598.1.
CP002685 Genomic DNA. Translation: AEC09600.1.
CP002685 Genomic DNA. Translation: AEC09602.1.
BT004266 mRNA. Translation: AAO42268.1.
BT005536 mRNA. Translation: AAO63956.1.
AY088554 mRNA. Translation: AAM66086.1.
PIRiE84810.
RefSeqiNP_001031511.1. NM_001036434.1. [Q9SLG0-1]
NP_030436.1. NM_129445.2. [Q9SLG0-1]
NP_850304.2. NM_179973.3. [Q9SLG0-1]
UniGeneiAt.24820.

3D structure databases

ProteinModelPortaliQ9SLG0.
SMRiQ9SLG0. Positions 20-110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3812. 15 interactions.
IntActiQ9SLG0. 4 interactions.

PTM databases

iPTMnetiQ9SLG0.

Proteomic databases

PRIDEiQ9SLG0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G38880.1; AT2G38880.1; AT2G38880. [Q9SLG0-1]
AT2G38880.2; AT2G38880.2; AT2G38880. [Q9SLG0-1]
AT2G38880.5; AT2G38880.5; AT2G38880. [Q9SLG0-1]
GeneIDi818472.

Organism-specific databases

TAIRiAT2G38880.

Phylogenomic databases

HOGENOMiHOG000211832.
InParanoidiQ9SLG0.
PhylomeDBiQ9SLG0.

Enzyme and pathway databases

ReactomeiR-ATH-2426168. Activation of gene expression by SREBF (SREBP).

Miscellaneous databases

PROiQ9SLG0.

Gene expression databases

ExpressionAtlasiQ9SLG0. baseline and differential.
GenevisibleiQ9SLG0. AT.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
IPR003956. Transcrpt_fac_NFYB/HAP3_CS.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00685. NFYB_HAP3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple genes encoding the conserved CCAAT-box transcription factor complex are expressed in Arabidopsis."
    Edwards D., Murray J.A.H., Smith A.G.
    Plant Physiol. 117:1015-1022(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Regulation of the CCAAT-binding NF-Y subunits in Arabidopsis thaliana."
    Gusmaroli G., Tonelli C., Mantovani R.
    Gene 264:173-185(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Regulation of novel members of the Arabidopsis thaliana CCAAT-binding nuclear factor Y subunits."
    Gusmaroli G., Tonelli C., Mantovani R.
    Gene 283:41-48(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNFYB1_ARATH
AccessioniPrimary (citable) accession number: Q9SLG0
Secondary accession number(s): O23633
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: June 1, 2002
Last modified: February 17, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.