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Q9SLF3 (TC132_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Translocase of chloroplast 132, chloroplastic

Short name=AtToc132
EC=3.6.5.-
Alternative name(s):
132 kDa chloroplast outer envelope protein
Gene names
Name:TOC132
Ordered Locus Names:At2g16640
ORF Names:T24I21.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1206 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Probably specialized in the import of nuclear encoded non-photosynthetic preproteins from the cytoplasm to the chloroplast. Ref.6 Ref.7

Cofactor

Binds 1 magnesium ion by subunit By similarity.

Subunit structure

Homodimer By similarity. Part of the TOC core complex that includes 1 protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursor such as prSS (precursor of the RuBisCO small subunit) interacts with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). Ref.5

Subcellular location

Plastidchloroplast outer membrane; Single-pass membrane protein By similarity. Cytoplasm By similarity. Note: Cycles between the cytoplasm and chloroplast, probably as a soluble preprotein receptor. The anchoring to the chloroplast outer membrane required the GTPase activity and GDP. May contains beta barrel transmembrane regions By similarity.

Tissue specificity

Expressed in seedlings, leaves, flowers, and roots. Ref.6

Induction

By light conditions. Ref.5

Post-translational modification

Phosphorylated By similarity.

Sequence similarities

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. TOC159 subfamily.

Contains 1 AIG1-type G (guanine nucleotide-binding) domain.

Sequence caution

The sequence AAM20511.1 differs from that shown. Reason: Erroneous termination at position 1203. Translated as Gln.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 12061205Translocase of chloroplast 132, chloroplastic
PRO_0000352658

Regions

Transmembrane1182 – 119918Helical; Potential
Domain572 – 801230AIG1-type G
Nucleotide binding584 – 5896GTP By similarity
Nucleotide binding603 – 6086GTP By similarity
Nucleotide binding749 – 7502GTP By similarity
Region603 – 6064Homodimerization By similarity
Region666 – 6716Homodimerization By similarity
Coiled coil13 – 3321 Potential
Compositional bias44 – 305262Glu-rich

Sites

Metal binding5881Magnesium By similarity
Binding site7011GTP; via amide nitrogen By similarity

Amino acid modifications

Modified residue21N-acetylglycine Ref.9
Modified residue1951Phosphoserine Ref.8
Modified residue3371Phosphoserine By similarity
Modified residue3631Phosphoserine By similarity
Modified residue3981Phosphoserine By similarity

Experimental info

Sequence conflict101R → G in AAM20511. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9SLF3 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: FEFB80CFC83655B5

FASTA1,206132,277
        10         20         30         40         50         60 
MGDGTEFVVR SDREDKKLAE DRISDEQVVK NELVRSDEVR DDNEDEVFEE AIGSENDEQE 

        70         80         90        100        110        120 
EEEDPKRELF ESDDLPLVET LKSSMVEHEV EDFEEAVGDL DETSSNEGGV KDFTAVGESH 

       130        140        150        160        170        180 
GAGEAEFDVL ATKMNGDKGE GGGGGSYDKV ESSLDVVDTT ENATSTNTNG SNLAAEHVGI 

       190        200        210        220        230        240 
ENGKTHSFLG NGIASPKNKE VVAEVIPKDD GIEEPWNDGI EVDNWEERVD GIQTEQEVEE 

       250        260        270        280        290        300 
GEGTTENQFE KRTEEEVVEG EGTSKNLFEK QTEQDVVEGE GTSKDLFENG SVCMDSESEA 

       310        320        330        340        350        360 
ERNGETGAAY TSNIVTNASG DNEVSSAVTS SPLEESSSGE KGETEGDSTC LKPEQHLASS 

       370        380        390        400        410        420 
PHSYPESTEV HSNSGSPGVT SREHKPVQSA NGGHDVQSPQ PNKELEKQQS SRVHVDPEIT 

       430        440        450        460        470        480 
ENSHVETEPE VVSSVSPTES RSNPAALPPA RPAGLGRASP LLEPASRAPQ QSRVNGNGSH 

       490        500        510        520        530        540 
NQFQQAEDST TTEADEHDET REKLQLIRVK FLRLAHRLGQ TPHNVVVAQV LYRLGLAEQL 

       550        560        570        580        590        600 
RGRNGSRVGA FSFDRASAMA EQLEAAGQDP LDFSCTIMVL GKSGVGKSAT INSIFDEVKF 

       610        620        630        640        650        660 
CTDAFQMGTK RVQDVEGLVQ GIKVRVIDTP GLLPSWSDQA KNEKILNSVK AFIKKNPPDI 

       670        680        690        700        710        720 
VLYLDRLDMQ SRDSGDMPLL RTISDVFGPS IWFNAIVGLT HAASVPPDGP NGTASSYDMF 

       730        740        750        760        770        780 
VTQRSHVIQQ AIRQAAGDMR LMNPVSLVEN HSACRTNRAG QRVLPNGQVW KPHLLLLSFA 

       790        800        810        820        830        840 
SKILAEANAL LKLQDNIPGR PFAARSKAPP LPFLLSSLLQ SRPQPKLPEQ QYGDEEDEDD 

       850        860        870        880        890        900 
LEESSDSDEE SEYDQLPPFK SLTKAQMATL SKSQKKQYLD EMEYREKLLM KKQMKEERKR 

       910        920        930        940        950        960 
RKMFKKFAAE IKDLPDGYSE NVEEESGGPA SVPVPMPDLS LPASFDSDNP THRYRYLDSS 

       970        980        990       1000       1010       1020 
NQWLVRPVLE THGWDHDIGY EGVNAERLFV VKEKIPISVS GQVTKDKKDA NVQLEMASSV 

      1030       1040       1050       1060       1070       1080 
KHGEGKSTSL GFDMQTVGKE LAYTLRSETR FNNFRRNKAA AGLSVTHLGD SVSAGLKVED 

      1090       1100       1110       1120       1130       1140 
KFIASKWFRI VMSGGAMTSR GDFAYGGTLE AQLRDKDYPL GRFLTTLGLS VMDWHGDLAI 

      1150       1160       1170       1180       1190       1200 
GGNIQSQVPI GRSSNLIARA NLNNRGAGQV SVRVNSSEQL QLAMVAIVPL FKKLLSYYYP 


QTQYGQ 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 728-1206.
Strain: cv. Columbia.
[5]"The major protein import receptor of plastids is essential for chloroplast biogenesis."
Bauer J., Chen K., Hiltbunner A., Wehrli E., Eugster M., Schnell D., Kessler F.
Nature 403:203-207(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH THE TOC COMPLEX, INDUCTION BY LIGHT.
[6]"Functional specialization amongst the Arabidopsis Toc159 family of chloroplast protein import receptors."
Kubis S., Patel R., Combe J., Bedard J., Kovacheva S., Lilley K., Biehl A., Leister D., Rios G., Koncz C., Jarvis P.
Plant Cell 16:2059-2077(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]"Deletion of core components of the plastid protein import machinery causes differential arrest of embryo development in Arabidopsis thaliana."
Hust B., Gutensohn M.
Plant Biol. 8:18-30(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
[9]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT GLY-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC005825 Genomic DNA. Translation: AAD24598.1.
CP002685 Genomic DNA. Translation: AEC06521.1.
AY099660 mRNA. Translation: AAM20511.1. Sequence problems.
AK222043 mRNA. Translation: BAD94786.1.
PIRD84542.
RefSeqNP_179255.1. NM_127216.4.
UniGeneAt.21953.
At.67099.

3D structure databases

DisProtDP00610.
ProteinModelPortalQ9SLF3.
SMRQ9SLF3. Positions 543-775.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid1522. 3 interactions.
IntActQ9SLF3. 5 interactions.

Proteomic databases

PaxDbQ9SLF3.
PRIDEQ9SLF3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G16640.1; AT2G16640.1; AT2G16640.
GeneID816165.
KEGGath:AT2G16640.

Organism-specific databases

TAIRAT2G16640.

Phylogenomic databases

eggNOGNOG244338.
HOGENOMHOG000243570.
InParanoidQ9SLF3.
OMAIENGKTH.
PhylomeDBQ9SLF3.

Gene expression databases

GenevestigatorQ9SLF3.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR006703. AIG1.
IPR024283. DUF3406_chlpt_translocase.
IPR027417. P-loop_NTPase.
IPR005690. Toc86_159.
[Graphical view]
PfamPF04548. AIG1. 1 hit.
PF11886. DUF3406. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00993. 3a0901s04IAP86. 1 hit.
PROSITEPS51720. G_AIG1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTC132_ARATH
AccessionPrimary (citable) accession number: Q9SLF3
Secondary accession number(s): Q56WJ7, Q8LPK1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names