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Q9SLA8

- FABI_ARATH

UniProt

Q9SLA8 - FABI_ARATH

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Protein
Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic
Gene
MOD1, ENR-A, ENR1, At2g05990, T6P5.19
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Catalyzes the last reduction step in the de novo synthesis cycle of fatty acids. Involved in the elongation cycle of fatty acids which are used in lipid metabolism. Required for normal plant growth.2 Publications

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Enzyme regulationi

Inhibited by the phytotoxin cyperin and the synthetic antimicrobial compound triclosan.1 Publication

Kineticsi

  1. KM=288 µM for crotonyl-CoA1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011NAD; via carbonyl oxygen By similarity
Binding sitei108 – 1081NAD By similarity
Binding sitei262 – 2621NAD; via carbonyl oxygen By similarity
Active sitei264 – 2641Proton acceptor By similarity
Active sitei274 – 2741Proton acceptor By similarity
Binding sitei282 – 2821NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi165 – 1662NAD By similarity
Nucleotide bindingi212 – 2132NAD By similarity
Nucleotide bindingi312 – 3165NAD By similarity

GO - Molecular functioni

  1. copper ion binding Source: TAIR
  2. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB-EC
  3. enoyl-[acyl-carrier-protein] reductase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. fatty acid biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT2G05990-MONOMER.
ARA:GQT-2200-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase 1
Protein MOSAIC DEATH 1
Gene namesi
Name:MOD1
Synonyms:ENR-A, ENR1
Ordered Locus Names:At2g05990
ORF Names:T6P5.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G05990.

Subcellular locationi

Plastidchloroplast Reviewed prediction

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast stroma Source: TAIR
  4. fatty acid synthase complex Source: TAIR
  5. thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Premature cell death in several organs, chlorotic and curly leaves, semidwarfism, distorted siliques, premature senescence of primary inflorescences, reduced fertility and decrease in total lipid content.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi226 – 2261T → I: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7474Chloroplast Reviewed prediction
Add
BLAST
Chaini75 – 390316Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic
PRO_0000420278Add
BLAST

Proteomic databases

PRIDEiQ9SLA8.

Expressioni

Tissue specificityi

Expressed in flowers and siliques and at lower levels in roots and leaves (at protein level).2 Publications

Gene expression databases

GenevestigatoriQ9SLA8.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

IntActiQ9SLA8. 1 interaction.
STRINGi3702.AT2G05990.2-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SLA8.
SMRiQ9SLA8. Positions 87-383.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiQ9SLA8.
KOiK00208.
OMAiTGEIHHV.
PhylomeDBiQ9SLA8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SLA8-1 [UniParc]FASTAAdd to Basket

« Hide

MAATAASSLQ IATRRPSMSS PSKILKAGTY IVGANPGNAS WDKLSCTRQL    50
SNLGCLRNHS AVPTCKRPFS FSTRAMSESS ENKAPSGLPI DLRGKRAFIA 100
GIADDNGYGW AIAKSLAAAG AEILVGTWVP ALNIFETSLR RGKFDQSRVL 150
PDGSLMEIKK VYALDAVFDN PEDVPEDVKT NKRYAGSSNW TVQEAAECVK 200
KDFGSIDILV HSLANGPEVS KPLLETSRKG YLAAISASSY SFVSLLRHFL 250
PIMNPGGASI SLTYIASERI IPGYGGGMSS AKAALESDTR VLAYEAGRKS 300
NIRVNTISAG PLGSRAAKAI GFIDTMIEYS YNNGPIQKTL TADEVGNAAA 350
FLASPLASAI TGATIYVDNG LNAMGVALDS PVFKDLNSKN 390
Length:390
Mass (Da):41,214
Last modified:May 1, 2000 - v1
Checksum:i95E549A6E64BE5BC
GO

Sequence cautioni

The sequence AAG40070.1 differs from that shown. Reason: Frameshift at position 150.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241I → V in CAA74175. 1 Publication
Sequence conflicti35 – 351N → Y in CAA74175. 1 Publication
Sequence conflicti59 – 602HS → NT in CAA74175. 1 Publication
Sequence conflicti234 – 2341A → V in CAA74175. 1 Publication
Sequence conflicti376 – 3761V → R in AAF37208. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13860 Genomic DNA. Translation: CAA74175.1.
AF207593 mRNA. Translation: AAF37208.1.
AC005970 Genomic DNA. Translation: AAC95176.1.
CP002685 Genomic DNA. Translation: AEC05988.1.
CP002685 Genomic DNA. Translation: AEC05989.1.
AF324719 mRNA. Translation: AAG40070.1. Frameshift.
AY056192 mRNA. Translation: AAL07041.1.
AY113962 mRNA. Translation: AAM45010.1.
PIRiH84473.
RefSeqiNP_565331.1. NM_126612.2.
NP_849940.1. NM_179609.1.
UniGeneiAt.23842.

Genome annotation databases

EnsemblPlantsiAT2G05990.1; AT2G05990.1; AT2G05990.
AT2G05990.2; AT2G05990.2; AT2G05990.
GeneIDi815152.
KEGGiath:AT2G05990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y13860 Genomic DNA. Translation: CAA74175.1 .
AF207593 mRNA. Translation: AAF37208.1 .
AC005970 Genomic DNA. Translation: AAC95176.1 .
CP002685 Genomic DNA. Translation: AEC05988.1 .
CP002685 Genomic DNA. Translation: AEC05989.1 .
AF324719 mRNA. Translation: AAG40070.1 . Frameshift.
AY056192 mRNA. Translation: AAL07041.1 .
AY113962 mRNA. Translation: AAM45010.1 .
PIRi H84473.
RefSeqi NP_565331.1. NM_126612.2.
NP_849940.1. NM_179609.1.
UniGenei At.23842.

3D structure databases

ProteinModelPortali Q9SLA8.
SMRi Q9SLA8. Positions 87-383.
ModBasei Search...

Protein-protein interaction databases

IntActi Q9SLA8. 1 interaction.
STRINGi 3702.AT2G05990.2-P.

Proteomic databases

PRIDEi Q9SLA8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G05990.1 ; AT2G05990.1 ; AT2G05990 .
AT2G05990.2 ; AT2G05990.2 ; AT2G05990 .
GeneIDi 815152.
KEGGi ath:AT2G05990.

Organism-specific databases

TAIRi AT2G05990.

Phylogenomic databases

InParanoidi Q9SLA8.
KOi K00208.
OMAi TGEIHHV.
PhylomeDBi Q9SLA8.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci ARA:AT2G05990-MONOMER.
ARA:GQT-2200-MONOMER.

Gene expression databases

Genevestigatori Q9SLA8.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequences surrounding the transcription initiation site of the Arabidopsis enoyl-acyl carrier protein reductase gene control seed expression in transgenic tobacco."
    de Boer G.J., Testerink C., Pielage G., Nijkamp H.J., Stuitje A.R.
    Plant Mol. Biol. 39:1197-1207(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
  2. "Deficiency in fatty acid synthase leads to premature cell death and dramatic alterations in plant morphology."
    Mou Z., He Y., Dai Y., Liu X., Li J.
    Plant Cell 12:405-418(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF THR-226.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "A pathogenic fungi diphenyl ether phytotoxin targets plant enoyl (acyl carrier protein) reductase."
    Dayan F.E., Ferreira D., Wang Y.H., Khan I.A., McInroy J.A., Pan Z.
    Plant Physiol. 147:1062-1071(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiFABI_ARATH
AccessioniPrimary (citable) accession number: Q9SLA8
Secondary accession number(s): O04942, Q9FEF2, Q9M672
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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