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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic

Gene

MOD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Catalyzes the last reduction step in the de novo synthesis cycle of fatty acids. Involved in the elongation cycle of fatty acids which are used in lipid metabolism. Required for normal plant growth.2 Publications

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Enzyme regulationi

Inhibited by the phytotoxin cyperin and the synthetic antimicrobial compound triclosan.1 Publication

Kineticsi

  1. KM=288 µM for crotonyl-CoA1 Publication

    Pathwayi: fatty acid biosynthesis

    This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
    View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei101NAD; via carbonyl oxygenBy similarity1
    Binding sitei108NADBy similarity1
    Binding sitei262NAD; via carbonyl oxygenBy similarity1
    Active sitei264Proton acceptorBy similarity1
    Active sitei274Proton acceptorBy similarity1
    Binding sitei282NADBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi165 – 166NADBy similarity2
    Nucleotide bindingi212 – 213NADBy similarity2
    Nucleotide bindingi312 – 316NADBy similarity5

    GO - Molecular functioni

    • copper ion binding Source: TAIR
    • enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB-EC
    • enoyl-[acyl-carrier-protein] reductase activity Source: TAIR

    GO - Biological processi

    • fatty acid biosynthetic process Source: TAIR

    Keywordsi

    Molecular functionOxidoreductase
    Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
    LigandNAD

    Enzyme and pathway databases

    BioCyciARA:AT2G05990-MONOMER
    UniPathwayiUPA00094

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic (EC:1.3.1.9)
    Short name:
    ENR
    Alternative name(s):
    NADH-dependent enoyl-ACP reductase 1
    Protein MOSAIC DEATH 1
    Gene namesi
    Name:MOD1
    Synonyms:ENR-A, ENR1
    Ordered Locus Names:At2g05990
    ORF Names:T6P5.19
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    AraportiAT2G05990
    TAIRilocus:2064681 AT2G05990

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Premature cell death in several organs, chlorotic and curly leaves, semidwarfism, distorted siliques, premature senescence of primary inflorescences, reduced fertility and decrease in total lipid content.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi226T → I: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 74ChloroplastSequence analysisAdd BLAST74
    ChainiPRO_000042027875 – 390Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplasticAdd BLAST316

    Proteomic databases

    PaxDbiQ9SLA8
    PRIDEiQ9SLA8
    ProMEXiQ9SLA8

    PTM databases

    iPTMnetiQ9SLA8

    Expressioni

    Tissue specificityi

    Expressed in flowers and siliques and at lower levels in roots and leaves (at protein level).2 Publications

    Gene expression databases

    ExpressionAtlasiQ9SLA8 baseline and differential
    GenevisibleiQ9SLA8 AT

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    BioGridi551, 1 interactor
    IntActiQ9SLA8, 1 interactor
    STRINGi3702.AT2G05990.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SLA8
    SMRiQ9SLA8
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiENOG410IQSQ Eukaryota
    COG0623 LUCA
    InParanoidiQ9SLA8
    KOiK00208
    OMAiSVATAMH
    OrthoDBiEOG09360GOD
    PhylomeDBiQ9SLA8

    Family and domain databases

    InterProiView protein in InterPro
    IPR036291 NAD(P)-bd_dom_sf
    IPR002347 SDR_fam
    PRINTSiPR00081 GDHRDH
    SUPFAMiSSF51735 SSF51735, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SLA8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAATAASSLQ IATRRPSMSS PSKILKAGTY IVGANPGNAS WDKLSCTRQL
    60 70 80 90 100
    SNLGCLRNHS AVPTCKRPFS FSTRAMSESS ENKAPSGLPI DLRGKRAFIA
    110 120 130 140 150
    GIADDNGYGW AIAKSLAAAG AEILVGTWVP ALNIFETSLR RGKFDQSRVL
    160 170 180 190 200
    PDGSLMEIKK VYALDAVFDN PEDVPEDVKT NKRYAGSSNW TVQEAAECVK
    210 220 230 240 250
    KDFGSIDILV HSLANGPEVS KPLLETSRKG YLAAISASSY SFVSLLRHFL
    260 270 280 290 300
    PIMNPGGASI SLTYIASERI IPGYGGGMSS AKAALESDTR VLAYEAGRKS
    310 320 330 340 350
    NIRVNTISAG PLGSRAAKAI GFIDTMIEYS YNNGPIQKTL TADEVGNAAA
    360 370 380 390
    FLASPLASAI TGATIYVDNG LNAMGVALDS PVFKDLNSKN
    Length:390
    Mass (Da):41,214
    Last modified:May 1, 2000 - v1
    Checksum:i95E549A6E64BE5BC
    GO

    Sequence cautioni

    The sequence AAG40070 differs from that shown. Reason: Frameshift at position 150.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti24I → V in CAA74175 (PubMed:10380806).Curated1
    Sequence conflicti35N → Y in CAA74175 (PubMed:10380806).Curated1
    Sequence conflicti59 – 60HS → NT in CAA74175 (PubMed:10380806).Curated2
    Sequence conflicti234A → V in CAA74175 (PubMed:10380806).Curated1
    Sequence conflicti376V → R in AAF37208 (PubMed:10380806).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y13860 Genomic DNA Translation: CAA74175.1
    AF207593 mRNA Translation: AAF37208.1
    AC005970 Genomic DNA Translation: AAC95176.1
    CP002685 Genomic DNA Translation: AEC05988.1
    CP002685 Genomic DNA Translation: AEC05989.1
    AF324719 mRNA Translation: AAG40070.1 Frameshift.
    AY056192 mRNA Translation: AAL07041.1
    AY113962 mRNA Translation: AAM45010.1
    PIRiH84473
    RefSeqiNP_565331.1, NM_126612.3
    NP_849940.1, NM_179609.1
    UniGeneiAt.23842

    Genome annotation databases

    EnsemblPlantsiAT2G05990.1; AT2G05990.1; AT2G05990
    AT2G05990.2; AT2G05990.2; AT2G05990
    GeneIDi815152
    GrameneiAT2G05990.1; AT2G05990.1; AT2G05990
    AT2G05990.2; AT2G05990.2; AT2G05990
    KEGGiath:AT2G05990

    Similar proteinsi

    Entry informationi

    Entry nameiFABI_ARATH
    AccessioniPrimary (citable) accession number: Q9SLA8
    Secondary accession number(s): O04942, Q9FEF2, Q9M672
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: May 1, 2000
    Last modified: April 25, 2018
    This is version 122 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health