ID   P2C22_ARATH             Reviewed;         392 AA.
AC   Q9SLA1;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   24-JAN-2024, entry version 145.
DE   RecName: Full=Probable protein phosphatase 2C 22;
DE            Short=AtPP2C22;
DE            EC=3.1.3.16;
GN   OrderedLocusNames=At2g25620; ORFNames=F3N11.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA   Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT   "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT   Arabidopsis.";
RL   BMC Genomics 9:550-550(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:83421; EC=3.1.3.16;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC         COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:61977; EC=3.1.3.16;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC   -!- INTERACTION:
CC       Q9SLA1; Q9FTA2: TCP21; NbExp=3; IntAct=EBI-15193303, EBI-4426168;
CC       Q9SLA1; Q8LPR5: TCP4; NbExp=3; IntAct=EBI-15193303, EBI-15192325;
CC   -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
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DR   EMBL; AC006053; AAD31375.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC07725.1; -; Genomic_DNA.
DR   EMBL; CP002685; ANM61961.1; -; Genomic_DNA.
DR   EMBL; AF326901; AAG41483.1; -; mRNA.
DR   EMBL; AF339719; AAK00401.1; -; mRNA.
DR   EMBL; AF436827; AAL32009.1; -; mRNA.
DR   EMBL; AY039595; AAK62650.1; -; mRNA.
DR   EMBL; AY057740; AAL15370.1; -; mRNA.
DR   EMBL; BT002487; AAO00847.1; -; mRNA.
DR   PIR; F84650; F84650.
DR   RefSeq; NP_001324148.1; NM_001336005.1.
DR   RefSeq; NP_180133.1; NM_128120.2.
DR   AlphaFoldDB; Q9SLA1; -.
DR   SMR; Q9SLA1; -.
DR   BioGRID; 2454; 48.
DR   IntAct; Q9SLA1; 46.
DR   STRING; 3702.Q9SLA1; -.
DR   iPTMnet; Q9SLA1; -.
DR   PaxDb; 3702-AT2G25620-1; -.
DR   ProteomicsDB; 248874; -.
DR   EnsemblPlants; AT2G25620.1; AT2G25620.1; AT2G25620.
DR   EnsemblPlants; AT2G25620.2; AT2G25620.2; AT2G25620.
DR   GeneID; 817102; -.
DR   Gramene; AT2G25620.1; AT2G25620.1; AT2G25620.
DR   Gramene; AT2G25620.2; AT2G25620.2; AT2G25620.
DR   KEGG; ath:AT2G25620; -.
DR   Araport; AT2G25620; -.
DR   TAIR; AT2G25620; DBP1.
DR   eggNOG; KOG0698; Eukaryota.
DR   HOGENOM; CLU_013173_21_2_1; -.
DR   InParanoid; Q9SLA1; -.
DR   OMA; RDHKPIC; -.
DR   OrthoDB; 91820at2759; -.
DR   PhylomeDB; Q9SLA1; -.
DR   PRO; PR:Q9SLA1; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q9SLA1; baseline and differential.
DR   GO; GO:0005829; C:cytosol; IDA:TAIR.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050688; P:regulation of defense response to virus; IMP:TAIR.
DR   CDD; cd00143; PP2Cc; 1.
DR   Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR   InterPro; IPR015655; PP2C.
DR   InterPro; IPR000222; PP2C_BS.
DR   InterPro; IPR036457; PPM-type-like_dom_sf.
DR   InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR   PANTHER; PTHR13832; PROTEIN PHOSPHATASE 2C; 1.
DR   PANTHER; PTHR13832:SF790; PROTEIN PHOSPHATASE 2C 22-RELATED; 1.
DR   Pfam; PF00481; PP2C; 1.
DR   SMART; SM00332; PP2Cc; 1.
DR   SUPFAM; SSF81606; PP2C-like; 1.
DR   PROSITE; PS01032; PPM_1; 1.
DR   PROSITE; PS51746; PPM_2; 1.
DR   Genevisible; Q9SLA1; AT.
PE   1: Evidence at protein level;
KW   Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase;
KW   Reference proteome.
FT   CHAIN           1..392
FT                   /note="Probable protein phosphatase 2C 22"
FT                   /id="PRO_0000367952"
FT   DOMAIN          89..356
FT                   /note="PPM-type phosphatase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        8..26
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         133
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         133
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         134
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         304
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         347
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   392 AA;  42674 MW;  AC09DC922197BE53 CRC64;
     MEETRGISDP ENGSSSYGGK PPNPLSFSSS SAAAAVYRQT FDGERSLAPC NKRSLVRHSS
     LVKTMVSDIS VENEFTIEKN KSEFVPATRS GAWSDIGSRS SMEDAYLCVD NFMDSFGLLN
     SEAGPSAFYG VFDGHGGKHA AEFACHHIPR YIVEDQEFPS EINKVLSSAF LQTDTAFLEA
     CSLDGSLASG TTALAAILFG RSLVVANAGD CRAVLSRQGK AIEMSRDHKP MSSKERRRIE
     ASGGHVFDGY LNGQLNVARA LGDFHMEGMK KKKDGSDCGP LIAEPELMTT KLTEEDEFLI
     IGCDGVWDVF MSQNAVDFAR RRLQEHNDPV MCSKELVEEA LKRKSADNVT AVVVCLQPQP
     PPNLVAPRLR VHRSFSAEGL KDLQSYLDGL GN
//