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Protein

Molybdate transporter 1

Gene

MOT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High affinity molybdate transporter. Unable to transport sulfate.4 Publications

Enzyme regulationi

Not inhibited by sulfate.1 Publication

Kineticsi

  1. KM=21 nM for molybdate1 Publication

    GO - Molecular functioni

    • molybdate ion transmembrane transporter activity Source: TAIR

    GO - Biological processi

    • molybdate ion transport Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    Molybdenum

    Protein family/group databases

    TCDBi2.A.53.5.1. the sulfate permease (sulp) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Molybdate transporter 1
    Alternative name(s):
    Sulfate transporter like protein 5.2
    Gene namesi
    Name:MOT1
    Synonyms:ST5.2, SULTR5.2
    Ordered Locus Names:At2g25680
    ORF Names:F3N11.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    TAIRiAT2G25680.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei67 – 8721HelicalSequence analysisAdd
    BLAST
    Transmembranei110 – 13021HelicalSequence analysisAdd
    BLAST
    Transmembranei133 – 15321HelicalSequence analysisAdd
    BLAST
    Transmembranei177 – 19721HelicalSequence analysisAdd
    BLAST
    Transmembranei225 – 24521HelicalSequence analysisAdd
    BLAST
    Transmembranei309 – 32921HelicalSequence analysisAdd
    BLAST
    Transmembranei354 – 37421HelicalSequence analysisAdd
    BLAST
    Transmembranei377 – 39721HelicalSequence analysisAdd
    BLAST
    Transmembranei417 – 43721HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • mitochondrial membrane Source: UniProtKB-SubCell
    • mitochondrion Source: TAIR
    • vacuole Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane, Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    90% and 80% reduction in molybdate content in shoots and roots respectively. Reduced growth of roots and shoots under conditions of limited molybdate supply. 9-fold reduction in molybdate content in leaf vacuoles. Decreased nitrate reductase activity due to a reduced molybdate uptake.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 456456Molybdate transporter 1PRO_0000417396Add
    BLAST

    Proteomic databases

    PaxDbiQ9SL95.
    PRIDEiQ9SL95.

    Expressioni

    Tissue specificityi

    Strongly expressed in roots. Detected in the vascular tissues of hypocotyls, in petioles and vascular tissues of cotyledons and leaves, in mesophyll cells, stamen, sepals and siliques.2 Publications

    Developmental stagei

    Not regulated during leaf senescence.1 Publication

    Gene expression databases

    GenevisibleiQ9SL95. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G25680.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SL95.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi202 – 2098Poly-Glu
    Compositional biasi323 – 38361Gly-richAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IFG6. Eukaryota.
    ENOG410XTGP. LUCA.
    HOGENOMiHOG000000822.
    InParanoidiQ9SL95.
    OMAiFAIPEIM.
    PhylomeDBiQ9SL95.

    Family and domain databases

    InterProiIPR031563. MOT1/MOT2.
    IPR030161. MOT1_plant.
    [Graphical view]
    PANTHERiPTHR31970:SF3. PTHR31970:SF3. 1 hit.
    PfamiPF16983. MFS_MOT1. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SL95-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MESQSQRGQH ETPKRSRFTG MFHKLKTNLV FRSKLAEING AMGDLGTYIP
    60 70 80 90 100
    IVLALTLAKD LDLGTTLIFT GIYNAITGAV YGVPMPVQPM KSIAAVAISS
    110 120 130 140 150
    TAEDFGIPEI MAAGICTGGI LFVLGISGLM QLVFNIIPLS VVRGIQLSQG
    160 170 180 190 200
    LAFAMSAVKY IRKEQNFSKS KSVGDRPWLG LDGLVLALVC VLFIVLVNGD
    210 220 230 240 250
    GEEEEEEEEG DGSRGRGRWG SVRKVIANVP SALLIFLLGV VLAFIRKPSI
    260 270 280 290 300
    VHDIKFGPSK MKIVRISRKA WRNGFLKGTV PQLPLSVLNS VVAVCKLSYD
    310 320 330 340 350
    LFPEKEFSAA SVSMTVGLMN MVGCWFGAMP TCHGAGGLAG QYKFGGRSGG
    360 370 380 390 400
    CVALLGVAKL VLGLVLGGSL VGILEKFPVG VLGALLLFAG VELAMAARDM
    410 420 430 440 450
    NTKGDAFVML MCTSVSLGSN AAIGFVAGDL LYVVLWMRNY GRAKPSSLPP

    QSGEHA
    Length:456
    Mass (Da):48,391
    Last modified:May 1, 2000 - v1
    Checksum:iA816D7C66B78FDD0
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti429 – 4291D → V in strain: cv. Landsberg erecta.

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC006053 Genomic DNA. Translation: AAD31368.1.
    CP002685 Genomic DNA. Translation: AEC07734.1.
    AK221594 mRNA. Translation: BAD95122.1.
    PIRiD84651.
    RefSeqiNP_180139.1. NM_128127.3.
    UniGeneiAt.49243.

    Genome annotation databases

    EnsemblPlantsiAT2G25680.1; AT2G25680.1; AT2G25680.
    GeneIDi817109.
    GrameneiAT2G25680.1; AT2G25680.1; AT2G25680.
    KEGGiath:AT2G25680.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC006053 Genomic DNA. Translation: AAD31368.1.
    CP002685 Genomic DNA. Translation: AEC07734.1.
    AK221594 mRNA. Translation: BAD95122.1.
    PIRiD84651.
    RefSeqiNP_180139.1. NM_128127.3.
    UniGeneiAt.49243.

    3D structure databases

    ProteinModelPortaliQ9SL95.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT2G25680.1.

    Protein family/group databases

    TCDBi2.A.53.5.1. the sulfate permease (sulp) family.

    Proteomic databases

    PaxDbiQ9SL95.
    PRIDEiQ9SL95.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G25680.1; AT2G25680.1; AT2G25680.
    GeneIDi817109.
    GrameneiAT2G25680.1; AT2G25680.1; AT2G25680.
    KEGGiath:AT2G25680.

    Organism-specific databases

    TAIRiAT2G25680.

    Phylogenomic databases

    eggNOGiENOG410IFG6. Eukaryota.
    ENOG410XTGP. LUCA.
    HOGENOMiHOG000000822.
    InParanoidiQ9SL95.
    OMAiFAIPEIM.
    PhylomeDBiQ9SL95.

    Miscellaneous databases

    PROiQ9SL95.

    Gene expression databases

    GenevisibleiQ9SL95. AT.

    Family and domain databases

    InterProiIPR031563. MOT1/MOT2.
    IPR030161. MOT1_plant.
    [Graphical view]
    PANTHERiPTHR31970:SF3. PTHR31970:SF3. 1 hit.
    PfamiPF16983. MFS_MOT1. 2 hits.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil."
      Tomatsu H., Takano J., Takahashi H., Watanabe-Takahashi A., Shibagaki N., Fujiwara T.
      Proc. Natl. Acad. Sci. U.S.A. 104:18807-18812(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: cv. Columbia and cv. Landsberg erecta.
    5. "Variation in molybdenum content across broadly distributed populations of Arabidopsis thaliana is controlled by a mitochondrial molybdenum transporter (MOT1)."
      Baxter I., Muthukumar B., Park H.C., Buchner P., Lahner B., Danku J., Zhao K., Lee J., Hawkesford M.J., Guerinot M.L., Salt D.E.
      PLoS Genet. 4:E1000004-E1000004(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "Effects of molybdenum deficiency and defects in molybdate transporter MOT1 on transcript accumulation and nitrogen/sulphur metabolism in Arabidopsis thaliana."
      Ide Y., Kusano M., Oikawa A., Fukushima A., Tomatsu H., Saito K., Hirai M.Y., Fujiwara T.
      J. Exp. Bot. 62:1483-1497(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
    7. "Identification of an Arabidopsis solute carrier critical for intracellular transport and inter-organ allocation of molybdate."
      Gasber A., Klaumann S., Trentmann O., Trampczynska A., Clemens S., Schneider S., Sauer N., Feifer I., Bittner F., Mendel R.R., Neuhaus H.E.
      Plant Biol. 13:710-718(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiMOT1_ARATH
    AccessioniPrimary (citable) accession number: Q9SL95
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2012
    Last sequence update: May 1, 2000
    Last modified: February 17, 2016
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The amino-acid substitution Asp-429 to Val observed in cv. Landsberg erecta may decrease the molybdate transport activity. A 54-bp deletion in the promoter of MOT1 is strongly associated with the decreased expression of the gene and the resulting low molybdate content observed in several cultivars.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.