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Protein

Biotin--protein ligase 1, chloroplastic

Gene

HCS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a major role in biotin-dependent carboxylase biotinylation. Catalyzes the addition of biotin to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase and can also biotinylate methylcrotonyl-CoA carboxylase. Is responsible for most, if not all, biotin--protein ligase activity in Arabidopsis. Is essential for plant viability and required for ovule development.3 Publications

Miscellaneous

The alternative splicing of the 5'UTR of HCS1 mRNA controls the dual targeting of HCS1 protein through alternative use of distinct initiation codons. A small ORF (uORF24) located in the HCS1 mRNA 5'UTR is essential for the AUG choice. The presence of uORF24 favors the synthesis of a short protein form initiated at the second AUG, which consequently localizes in the cytosol. In the absence of uORF24, the translation initiation begins at the first AUG, allowing the production of a HCS1 protein headed by a transit peptide (PubMed:18156294).1 Publication

Catalytic activityi

ATP + biotin + apo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei145BiotinBy similarity1
Binding sitei220BiotinBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • biotin-[acetyl-CoA-carboxylase] ligase activity Source: TAIR
  • biotin-[methylcrotonoyl-CoA-carboxylase] ligase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G25710-MONOMER
MetaCyc:AT2G25710-MONOMER
ReactomeiR-ATH-196780 Biotin transport and metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin--protein ligase 1, chloroplastic (EC:6.3.4.-)
Alternative name(s):
Holocarboxylase synthetase 1
Including the following 2 domains:
Biotin--[methylcrotonoyl-CoA-carboxylase] ligase (EC:6.3.4.11)
Biotin--[acetyl-CoA-carboxylase] ligase (EC:6.3.4.15)
Gene namesi
Name:HCS1
Ordered Locus Names:At2g25710
ORF Names:F3N11.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G25710
TAIRilocus:2050291 AT2G25710

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Cytoplasm, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous due to aborted ovules that had not been fertilized.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37ChloroplastSequence analysisAdd BLAST37
ChainiPRO_000042597238 – 367Biotin--protein ligase 1, chloroplasticAdd BLAST330

Proteomic databases

PaxDbiQ9SL92

PTM databases

iPTMnetiQ9SL92

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, flowers, siliques and seeds.2 Publications

Gene expression databases

ExpressionAtlasiQ9SL92 baseline and differential
GenevisibleiQ9SL92 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G25710.1

Structurei

3D structure databases

ProteinModelPortaliQ9SL92
SMRiQ9SL92
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini105 – 289BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST185

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 123Biotin bindingBy similarity2
Regioni149 – 151Biotin bindingBy similarity3

Sequence similaritiesi

Belongs to the biotin--protein ligase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1536 Eukaryota
COG0340 LUCA
HOGENOMiHOG000264536
InParanoidiQ9SL92
KOiK01942
OMAiCAKGLSE
OrthoDBiEOG09360GI8
PhylomeDBiQ9SL92

Family and domain databases

CDDicd16442 BPL, 1 hit
InterProiView protein in InterPro
IPR004408 Biotin_CoA_COase_ligase
IPR003142 BPL_C
IPR004143 BPL_LPL_catalytic
PANTHERiPTHR12835:SF5 PTHR12835:SF5, 1 hit
PfamiView protein in Pfam
PF02237 BPL_C, 1 hit
PF03099 BPL_LplA_LipB, 1 hit
TIGRFAMsiTIGR00121 birA_ligase, 1 hit
PROSITEiView protein in PROSITE
PS51733 BPL_LPL_CATALYTIC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform HCS-ATG1 (identifier: Q9SL92-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAVRSTTTL SNFHLLNILV LRSLKPLHRL SFSFSASAME SDASCSLVLC
60 70 80 90 100
GKSSVETEVA KGLKNKNSLK LPDNTKVSLI LESEAKNLVK DDDNSFNLSL
110 120 130 140 150
FMNSIITHRF GRFLIWSPRL SSTHDVVSHN FSELPVGSVC VTDIQFKGRG
160 170 180 190 200
RTKNVWESPK GCLMYSFTLE MEDGRVVPLI QYVVSLAVTE AVKDVCDKKG
210 220 230 240 250
LPYIDVKIKW PNDLYVNGLK VGGILCTSTY RSKKFNVSVG VGLNVDNGQP
260 270 280 290 300
TTCLNAVLKG MAPESNLLKR EEILGAFFHK FEKFFDLFMD QGFKSLEELY
310 320 330 340 350
YRTWLHSEQR VIVEDKVEDQ VVQNVVTIQG LTSSGYLLAV GDDNQMYELH
360
PDGNSFDFFK GLVRRKI
Length:367
Mass (Da):41,219
Last modified:June 1, 2002 - v2
Checksum:iBB0192B4CD2EB5CF
GO
Isoform HCS-ATG2 (identifier: Q9SL92-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:329
Mass (Da):36,978
Checksum:i223BA9BA8E794A43
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28H → R in AAL93108 (PubMed:11784724).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0539161 – 38Missing in isoform HCS-ATG2. CuratedAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41369 mRNA Translation: AAC49706.1
AF414937 Genomic DNA Translation: AAL93108.1
AC006053 Genomic DNA Translation: AAD31371.2
CP002685 Genomic DNA Translation: AEC07740.1
CP002685 Genomic DNA Translation: AEC07741.1
CP002685 Genomic DNA Translation: ANM62688.1
CP002685 Genomic DNA Translation: ANM62689.1
BT024748 mRNA Translation: ABD59086.1
PIRiG84651
RefSeqiNP_001324829.1, NM_001336012.1 [Q9SL92-2]
NP_001324830.1, NM_001336013.1 [Q9SL92-2]
NP_565605.1, NM_128130.3 [Q9SL92-1]
NP_850067.1, NM_179736.1 [Q9SL92-1]
UniGeneiAt.19972

Genome annotation databases

EnsemblPlantsiAT2G25710.1; AT2G25710.1; AT2G25710 [Q9SL92-1]
AT2G25710.2; AT2G25710.2; AT2G25710 [Q9SL92-1]
AT2G25710.4; AT2G25710.4; AT2G25710 [Q9SL92-2]
AT2G25710.5; AT2G25710.5; AT2G25710 [Q9SL92-2]
GeneIDi817112
GrameneiAT2G25710.1; AT2G25710.1; AT2G25710 [Q9SL92-1]
AT2G25710.2; AT2G25710.2; AT2G25710 [Q9SL92-1]
AT2G25710.4; AT2G25710.4; AT2G25710 [Q9SL92-2]
AT2G25710.5; AT2G25710.5; AT2G25710 [Q9SL92-2]
KEGGiath:AT2G25710

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiHCS1_ARATH
AccessioniPrimary (citable) accession number: Q9SL92
Secondary accession number(s): P92975, Q8S4V8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health