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Q9SL76

- P2C19_ARATH

UniProt

Q9SL76 - P2C19_ARATH

Protein

Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein

Gene

At2g20050/At2g20040

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (14 Apr 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi148 – 1481Manganese 1By similarity
    Metal bindingi148 – 1481Manganese 2By similarity
    Metal bindingi149 – 1491Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi344 – 3441Manganese 2By similarity
    Metal bindingi388 – 3881Manganese 2By similarity
    Binding sitei811 – 8111ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi491 – 616126cNMP 1Add
    BLAST
    Nucleotide bindingi617 – 758142cNMP 2Add
    BLAST
    Nucleotide bindingi791 – 7999ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cAMP-dependent protein kinase regulator activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. protein serine/threonine kinase activity Source: UniProtKB-KW
    5. protein serine/threonine phosphatase activity Source: InterPro

    GO - Biological processi

    1. protein dephosphorylation Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G20050-MONOMER.
    ARA:GQT-1126-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein
    Including the following 2 domains:
    Probable protein phosphatase 2C 19 (EC:3.1.3.16)
    Short name:
    AtPP2C19
    Probable inactive cyclic nucleotide-dependent protein kinase At2g20050
    Gene namesi
    Ordered Locus Names:At2g20050/At2g20040
    ORF Names:T2G17.15/T2G17.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G20050.

    Subcellular locationi

    GO - Cellular componenti

    1. cAMP-dependent protein kinase complex Source: InterPro
    2. plasma membrane Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10941094Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing proteinPRO_0000367949Add
    BLAST

    Proteomic databases

    PaxDbiQ9SL76.
    PRIDEiQ9SL76.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9SL76.
    GenevestigatoriQ9SL76.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SL76.
    SMRiQ9SL76. Positions 123-394, 472-756, 777-1093.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini136 – 378243PP2C-likeAdd
    BLAST
    Domaini785 – 1038254Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The protein kinase domain is predicted to be catalytically inactive.

    Sequence similaritiesi

    In the N-terminal section; belongs to the PP2C family.Curated
    In the C-terminal section; belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.Curated
    Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
    Contains 1 PP2C-like domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0664.
    HOGENOMiHOG000030238.
    InParanoidiQ0WLT6.
    OMAiPAHRKTW.
    PhylomeDBiQ9SL76.

    Family and domain databases

    Gene3Di2.60.120.10. 2 hits.
    3.60.40.10. 1 hit.
    InterProiIPR002373. cAMP/cGMP_kin.
    IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR011009. Kinase-like_dom.
    IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR000719. Prot_kinase_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 2 hits.
    PF00069. Pkinase. 1 hit.
    PF00481. PP2C. 1 hit.
    [Graphical view]
    PRINTSiPR00103. CAMPKINASE.
    SMARTiSM00100. cNMP. 2 hits.
    SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 2 hits.
    SSF56112. SSF56112. 1 hit.
    SSF81606. SSF81606. 2 hits.
    PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
    PS01032. PP2C. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9SL76-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGCAYSKTCI GQICATKENS IRQTHQQAPS RGGTRATAAA AAVEEDNPVF     50
    NFSSDAVDDV DNDEIHQLGL SRDQEWGITR LSRVSSQFLP PDGSRVVKVP 100
    SCNYELRCSF LSQRGYYPDA LDKANQDSFA IHTPFGSNSD DHFFGVFDGH 150
    GEFGAQCSQF VKRRLCENLL RHGRFRVDPA EACNSAFLTT NSQLHADLVD 200
    DSMSGTTAIT VMVRGRTIYV ANAGDSRAVL AEKRDGDLVA VDLSIDQTPF 250
    RPDELERVKL CGARVLTLDQ IEGLKNPDVQ CWGTEEDDDG DPPRLWVPNG 300
    MYPGTAFTRS IGDSIAETIG VVANPEIAVV ELTPDNPFFV VASDGVFEFI 350
    SSQTVVDMVA KHKDPRDACA AIVAESYRLW LQYETRTDDI TIIVVHIDGL 400
    KDDAPRQLSS TGTQLQPPIP QVVELTGSES PSTFGWNSKN QRVRHDLSRA 450
    RIRAIENSLE NGHAWVPPSP AHRKTWEEEA HIERVLRDHF LFRKLTDSQC 500
    QVLLDCMQRL EANPGDIVVK QGGEGDCFYV VGSGEFEVLA TQDGKNGEVP 550
    RILQRYTAEK QSSFGELALM HNKPLQASVR AVDHGTLWAL KREDFRGILM 600
    SEFSNLASLK LLRSVDLLSR LTILQLSHVA ESLSEACFSD GQTIVTKDQK 650
    LQGLYVIQKG RVKISFCTEV LESQNVSSLT TGITNEYDNL EIGTEVSIEK 700
    HEGSYFGEWA LLGELKDSLS VVAVGEVVCV VLTKENFESA VGPLTNISDD 750
    GPKTRHSSFE LSKESAKVTD TTALAKATLA DLEWTTCLST TDCSEIGLVH 800
    LKDKENLLSL KRFSKQKVKK LGKEAQVLKE RNLMKNVIKP SAIVPEILCT 850
    CVDQTFAAIL LNTTLACPIS SLLHSPLDES SVRFITGSLV SAIEDIHKNE 900
    ILFRGSSPEL LMLDQSGYLQ IVDFRFAKKL SGERTFTICG NADYLAPEIV 950
    QGKGHGYAAD WWALGVLIYY MLEGEMPFGS WRESELDTFQ KIAKGQLTFP 1000
    RVLSSEAEDL ITKLLEVDEN LRFGSQGGPE SIKKHPWFNG LKWEAISNRE 1050
    FQVPQEIISR IHHHLENDNV LPLETSKSLD TTEDQDAQNW LEEW 1094
    Length:1,094
    Mass (Da):121,389
    Last modified:April 14, 2009 - v2
    Checksum:i89C6347D846297AC
    GO

    Sequence cautioni

    The sequence AAY27059.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAD24391.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g20040 and At2g20050.
    The sequence AAD24392.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g20040 and At2g20050.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU101468 mRNA. Translation: ABU68673.1.
    EU101469 mRNA. Translation: ABU68674.1.
    EU591510 mRNA. Translation: ACF05481.1.
    AC006081 Genomic DNA. Translation: AAD24391.1. Sequence problems.
    AC006081 Genomic DNA. Translation: AAD24392.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC06958.1.
    AK230102 mRNA. Translation: BAF01921.1.
    BT022072 mRNA. Translation: AAY27059.1. Different initiation.
    BT023477 mRNA. Translation: AAY57316.1.
    PIRiD84584.
    RefSeqiNP_179595.5. NM_127563.5. [Q9SL76-1]
    UniGeneiAt.47727.

    Genome annotation databases

    EnsemblPlantsiAT2G20050.1; AT2G20050.1; AT2G20050. [Q9SL76-1]
    GeneIDi816524.
    KEGGiath:AT2G20050.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU101468 mRNA. Translation: ABU68673.1 .
    EU101469 mRNA. Translation: ABU68674.1 .
    EU591510 mRNA. Translation: ACF05481.1 .
    AC006081 Genomic DNA. Translation: AAD24391.1 . Sequence problems.
    AC006081 Genomic DNA. Translation: AAD24392.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC06958.1 .
    AK230102 mRNA. Translation: BAF01921.1 .
    BT022072 mRNA. Translation: AAY27059.1 . Different initiation.
    BT023477 mRNA. Translation: AAY57316.1 .
    PIRi D84584.
    RefSeqi NP_179595.5. NM_127563.5. [Q9SL76-1 ]
    UniGenei At.47727.

    3D structure databases

    ProteinModelPortali Q9SL76.
    SMRi Q9SL76. Positions 123-394, 472-756, 777-1093.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9SL76.
    PRIDEi Q9SL76.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G20050.1 ; AT2G20050.1 ; AT2G20050 . [Q9SL76-1 ]
    GeneIDi 816524.
    KEGGi ath:AT2G20050.

    Organism-specific databases

    TAIRi AT2G20050.

    Phylogenomic databases

    eggNOGi COG0664.
    HOGENOMi HOG000030238.
    InParanoidi Q0WLT6.
    OMAi PAHRKTW.
    PhylomeDBi Q9SL76.

    Enzyme and pathway databases

    BioCyci ARA:AT2G20050-MONOMER.
    ARA:GQT-1126-MONOMER.

    Gene expression databases

    ArrayExpressi Q9SL76.
    Genevestigatori Q9SL76.

    Family and domain databases

    Gene3Di 2.60.120.10. 2 hits.
    3.60.40.10. 1 hit.
    InterProi IPR002373. cAMP/cGMP_kin.
    IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR011009. Kinase-like_dom.
    IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR000719. Prot_kinase_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 2 hits.
    PF00069. Pkinase. 1 hit.
    PF00481. PP2C. 1 hit.
    [Graphical view ]
    PRINTSi PR00103. CAMPKINASE.
    SMARTi SM00100. cNMP. 2 hits.
    SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 2 hits.
    SSF56112. SSF56112. 1 hit.
    SSF81606. SSF81606. 2 hits.
    PROSITEi PS50042. CNMP_BINDING_3. 2 hits.
    PS01032. PP2C. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Involvement of a putative cyclic nucleotide dependent hybrid phosphatase 2C/protein kinase gene in stress responses in higher plants."
      Van Ingelgem C., Azmi A., Inze D., Van Onckelen H., Roef L.
      Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Plants have cyclic nucleotide dependent kinases."
      Pascaud F., Corratge C., Thibaud J.-B., Lacombe B.
      Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 424-1090.
      Strain: cv. Columbia.
    6. "Arabidopsis ORF clones."
      Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 743-1090.
      Strain: cv. Columbia.
    7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiP2C19_ARATH
    AccessioniPrimary (citable) accession number: Q9SL76
    Secondary accession number(s): B5KQ16
    , B5KQ17, Q0WLT6, Q9SL77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 14, 2009
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3