Q9SL76 (P2C19_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein

Including the following 2 domains:

1. Probable protein phosphatase 2C 19
   Short name=AtPP2C19
   EC=3.1.3.16
2. Probable inactive cyclic nucleotide-dependent protein kinase At2g20050

Gene names

Ordered Locus Names:	At2g20050
ORF Names:	T2G17.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1094 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Domain	The protein kinase domain is predicted to be catalytically inactive.
Sequence similarities	In the N-terminal section; belongs to the PP2C family. In the C-terminal section; belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 2 cyclic nucleotide-binding domains. Contains 1 PP2C-like domain. Contains 1 protein kinase domain.
Sequence caution	The sequence AAD24391.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g20040 and At2g20050. The sequence AAD24392.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g20040 and At2g20050. The sequence AAY27059.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cAMP-dependent protein kinase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cAMP-dependent protein kinase regulator activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SL76-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1094	1094	Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein		PRO_0000367949
Regions
	Domain	136 – 378	243	PP2C-like
	Domain	785 – 1038	254	Protein kinase
	Nucleotide binding	491 – 616	126	cNMP 1
	Nucleotide binding	617 – 758	142	cNMP 2
	Nucleotide binding	791 – 799	9	ATP By similarity
Sites
	Metal binding	148	1	Manganese 1 By similarity
	Metal binding	148	1	Manganese 2 By similarity
	Metal binding	149	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	344	1	Manganese 2 By similarity
	Metal binding	388	1	Manganese 2 By similarity
	Binding site	811	1	ATP By similarity
Amino acid modifications
	Modified residue	758	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 14, 2009. Version 2. Checksum: 89C6347D846297AC	FASTA	1,094	121,389
	10 20 30 40 50 60 MGCAYSKTCI GQICATKENS IRQTHQQAPS RGGTRATAAA AAVEEDNPVF NFSSDAVDDV 70 80 90 100 110 120 DNDEIHQLGL SRDQEWGITR LSRVSSQFLP PDGSRVVKVP SCNYELRCSF LSQRGYYPDA 130 140 150 160 170 180 LDKANQDSFA IHTPFGSNSD DHFFGVFDGH GEFGAQCSQF VKRRLCENLL RHGRFRVDPA 190 200 210 220 230 240 EACNSAFLTT NSQLHADLVD DSMSGTTAIT VMVRGRTIYV ANAGDSRAVL AEKRDGDLVA 250 260 270 280 290 300 VDLSIDQTPF RPDELERVKL CGARVLTLDQ IEGLKNPDVQ CWGTEEDDDG DPPRLWVPNG 310 320 330 340 350 360 MYPGTAFTRS IGDSIAETIG VVANPEIAVV ELTPDNPFFV VASDGVFEFI SSQTVVDMVA 370 380 390 400 410 420 KHKDPRDACA AIVAESYRLW LQYETRTDDI TIIVVHIDGL KDDAPRQLSS TGTQLQPPIP 430 440 450 460 470 480 QVVELTGSES PSTFGWNSKN QRVRHDLSRA RIRAIENSLE NGHAWVPPSP AHRKTWEEEA 490 500 510 520 530 540 HIERVLRDHF LFRKLTDSQC QVLLDCMQRL EANPGDIVVK QGGEGDCFYV VGSGEFEVLA 550 560 570 580 590 600 TQDGKNGEVP RILQRYTAEK QSSFGELALM HNKPLQASVR AVDHGTLWAL KREDFRGILM 610 620 630 640 650 660 SEFSNLASLK LLRSVDLLSR LTILQLSHVA ESLSEACFSD GQTIVTKDQK LQGLYVIQKG 670 680 690 700 710 720 RVKISFCTEV LESQNVSSLT TGITNEYDNL EIGTEVSIEK HEGSYFGEWA LLGELKDSLS 730 740 750 760 770 780 VVAVGEVVCV VLTKENFESA VGPLTNISDD GPKTRHSSFE LSKESAKVTD TTALAKATLA 790 800 810 820 830 840 DLEWTTCLST TDCSEIGLVH LKDKENLLSL KRFSKQKVKK LGKEAQVLKE RNLMKNVIKP 850 860 870 880 890 900 SAIVPEILCT CVDQTFAAIL LNTTLACPIS SLLHSPLDES SVRFITGSLV SAIEDIHKNE 910 920 930 940 950 960 ILFRGSSPEL LMLDQSGYLQ IVDFRFAKKL SGERTFTICG NADYLAPEIV QGKGHGYAAD 970 980 990 1000 1010 1020 WWALGVLIYY MLEGEMPFGS WRESELDTFQ KIAKGQLTFP RVLSSEAEDL ITKLLEVDEN 1030 1040 1050 1060 1070 1080 LRFGSQGGPE SIKKHPWFNG LKWEAISNRE FQVPQEIISR IHHHLENDNV LPLETSKSLD 1090 TTEDQDAQNW LEEW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Involvement of a putative cyclic nucleotide dependent hybrid phosphatase 2C/protein kinase gene in stress responses in higher plants." Van Ingelgem C., Azmi A., Inze D., Van Onckelen H., Roef L. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Plants have cyclic nucleotide dependent kinases." Pascaud F., Corratge C., Thibaud J.-B., Lacombe B. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 424-1090. Strain: cv. Columbia.
[6]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R. Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 743-1090. Strain: cv. Columbia.
[7]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-758, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU101468 mRNA. Translation: ABU68673.1. EU101469 mRNA. Translation: ABU68674.1. EU591510 mRNA. Translation: ACF05481.1. AC006081 Genomic DNA. Translation: AAD24391.1. Sequence problems. AC006081 Genomic DNA. Translation: AAD24392.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC06958.1. AK230102 mRNA. Translation: BAF01921.1. BT022072 mRNA. Translation: AAY27059.1. Different initiation. BT023477 mRNA. Translation: AAY57316.1.
IPI	IPI00895636.
PIR	D84584.
RefSeq	NP_179595.5. NM_127563.5.
UniGene	At.47727.
3D structure databases
ProteinModelPortal	Q9SL76.
SMR	Q9SL76. Positions 123-394, 472-756, 777-1093.
ModBase	Search...
Proteomic databases
PaxDb	Q9SL76.
PRIDE	Q9SL76.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G20050.1; AT2G20050.1; AT2G20050.
GeneID	816524.
KEGG	ath:AT2G20050.
Organism-specific databases
TAIR	At2g20050.
Phylogenomic databases
eggNOG	COG0664.
HOGENOM	HOG000030238.
InParanoid	Q0WLT6.
KO	K04345.
OMA	AESYRLW.
PhylomeDB	Q9SL76.
ProtClustDB	CLSN2692644.
Gene expression databases
ArrayExpress	Q9SL76.
Genevestigator	Q9SL76.
Family and domain databases
Gene3D	2.60.120.10. 2 hits. 3.60.40.10. 1 hit.
InterPro	IPR002373. cAMP/cGMP_kin. IPR018490. cNMP-bd-like. IPR000595. cNMP-bd_dom. IPR011009. Kinase-like_dom. IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR000719. Prot_kinase_cat_dom. IPR015655. Protein_Pase_2C. IPR014710. RmlC-like_jellyroll. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00027. cNMP_binding. 2 hits. PF00069. Pkinase. 1 hit. PF00481. PP2C. 1 hit. [Graphical view]
PRINTS	PR00103. CAMPKINASE.
SMART	SM00100. cNMP. 2 hits. SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF51206. cNMP_binding. 2 hits. SSF56112. Kinase_like. 1 hit. SSF81606. PP2C-related. 1 hit.
PROSITE	PS00888. CNMP_BINDING_1. False negative. PS00889. CNMP_BINDING_2. False negative. PS50042. CNMP_BINDING_3. 2 hits. PS01032. PP2C. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C19_ARATH

Accession

Primary (citable) accession number: Q9SL76
Secondary accession number(s): B5KQ16 , B5KQ17, Q0WLT6, Q9SL77

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 14, 2009
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program