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Q9SL76 (P2C19_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein

Including the following 2 domains:

  1. Probable protein phosphatase 2C 19
    Short name=AtPP2C19
    EC=3.1.3.16
  2. Probable inactive cyclic nucleotide-dependent protein kinase At2g20050
Gene names
Ordered Locus Names:At2g20050/At2g20040
ORF Names:T2G17.15/T2G17.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1094 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Domain

The protein kinase domain is predicted to be catalytically inactive.

Sequence similarities

In the N-terminal section; belongs to the PP2C family.

In the C-terminal section; belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Contains 2 cyclic nucleotide-binding domains.

Contains 1 PP2C-like domain.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAD24391.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g20040 and At2g20050.

The sequence AAD24392.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g20040 and At2g20050.

The sequence AAY27059.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SL76-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10941094Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein
PRO_0000367949

Regions

Domain136 – 378243PP2C-like
Domain785 – 1038254Protein kinase
Nucleotide binding491 – 616126cNMP 1
Nucleotide binding617 – 758142cNMP 2
Nucleotide binding791 – 7999ATP By similarity

Sites

Metal binding1481Manganese 1 By similarity
Metal binding1481Manganese 2 By similarity
Metal binding1491Manganese 1; via carbonyl oxygen By similarity
Metal binding3441Manganese 2 By similarity
Metal binding3881Manganese 2 By similarity
Binding site8111ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 14, 2009. Version 2.
Checksum: 89C6347D846297AC

FASTA1,094121,389
        10         20         30         40         50         60 
MGCAYSKTCI GQICATKENS IRQTHQQAPS RGGTRATAAA AAVEEDNPVF NFSSDAVDDV 

        70         80         90        100        110        120 
DNDEIHQLGL SRDQEWGITR LSRVSSQFLP PDGSRVVKVP SCNYELRCSF LSQRGYYPDA 

       130        140        150        160        170        180 
LDKANQDSFA IHTPFGSNSD DHFFGVFDGH GEFGAQCSQF VKRRLCENLL RHGRFRVDPA 

       190        200        210        220        230        240 
EACNSAFLTT NSQLHADLVD DSMSGTTAIT VMVRGRTIYV ANAGDSRAVL AEKRDGDLVA 

       250        260        270        280        290        300 
VDLSIDQTPF RPDELERVKL CGARVLTLDQ IEGLKNPDVQ CWGTEEDDDG DPPRLWVPNG 

       310        320        330        340        350        360 
MYPGTAFTRS IGDSIAETIG VVANPEIAVV ELTPDNPFFV VASDGVFEFI SSQTVVDMVA 

       370        380        390        400        410        420 
KHKDPRDACA AIVAESYRLW LQYETRTDDI TIIVVHIDGL KDDAPRQLSS TGTQLQPPIP 

       430        440        450        460        470        480 
QVVELTGSES PSTFGWNSKN QRVRHDLSRA RIRAIENSLE NGHAWVPPSP AHRKTWEEEA 

       490        500        510        520        530        540 
HIERVLRDHF LFRKLTDSQC QVLLDCMQRL EANPGDIVVK QGGEGDCFYV VGSGEFEVLA 

       550        560        570        580        590        600 
TQDGKNGEVP RILQRYTAEK QSSFGELALM HNKPLQASVR AVDHGTLWAL KREDFRGILM 

       610        620        630        640        650        660 
SEFSNLASLK LLRSVDLLSR LTILQLSHVA ESLSEACFSD GQTIVTKDQK LQGLYVIQKG 

       670        680        690        700        710        720 
RVKISFCTEV LESQNVSSLT TGITNEYDNL EIGTEVSIEK HEGSYFGEWA LLGELKDSLS 

       730        740        750        760        770        780 
VVAVGEVVCV VLTKENFESA VGPLTNISDD GPKTRHSSFE LSKESAKVTD TTALAKATLA 

       790        800        810        820        830        840 
DLEWTTCLST TDCSEIGLVH LKDKENLLSL KRFSKQKVKK LGKEAQVLKE RNLMKNVIKP 

       850        860        870        880        890        900 
SAIVPEILCT CVDQTFAAIL LNTTLACPIS SLLHSPLDES SVRFITGSLV SAIEDIHKNE 

       910        920        930        940        950        960 
ILFRGSSPEL LMLDQSGYLQ IVDFRFAKKL SGERTFTICG NADYLAPEIV QGKGHGYAAD 

       970        980        990       1000       1010       1020 
WWALGVLIYY MLEGEMPFGS WRESELDTFQ KIAKGQLTFP RVLSSEAEDL ITKLLEVDEN 

      1030       1040       1050       1060       1070       1080 
LRFGSQGGPE SIKKHPWFNG LKWEAISNRE FQVPQEIISR IHHHLENDNV LPLETSKSLD 

      1090 
TTEDQDAQNW LEEW 

« Hide

References

« Hide 'large scale' references
[1]"Involvement of a putative cyclic nucleotide dependent hybrid phosphatase 2C/protein kinase gene in stress responses in higher plants."
Van Ingelgem C., Azmi A., Inze D., Van Onckelen H., Roef L.
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Plants have cyclic nucleotide dependent kinases."
Pascaud F., Corratge C., Thibaud J.-B., Lacombe B.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 424-1090.
Strain: cv. Columbia.
[6]"Arabidopsis ORF clones."
Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 743-1090.
Strain: cv. Columbia.
[7]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU101468 mRNA. Translation: ABU68673.1.
EU101469 mRNA. Translation: ABU68674.1.
EU591510 mRNA. Translation: ACF05481.1.
AC006081 Genomic DNA. Translation: AAD24391.1. Sequence problems.
AC006081 Genomic DNA. Translation: AAD24392.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06958.1.
AK230102 mRNA. Translation: BAF01921.1.
BT022072 mRNA. Translation: AAY27059.1. Different initiation.
BT023477 mRNA. Translation: AAY57316.1.
PIRD84584.
RefSeqNP_179595.5. NM_127563.5.
UniGeneAt.47727.

3D structure databases

ProteinModelPortalQ9SL76.
SMRQ9SL76. Positions 123-394, 472-756, 775-1070.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9SL76.
PRIDEQ9SL76.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G20050.1; AT2G20050.1; AT2G20050. [Q9SL76-1]
GeneID816524.
KEGGath:AT2G20050.

Organism-specific databases

TAIRAT2G20050.

Phylogenomic databases

eggNOGCOG0664.
HOGENOMHOG000030238.
InParanoidQ0WLT6.
OMACFYVVGS.
PhylomeDBQ9SL76.
ProtClustDBCLSN2692644.

Enzyme and pathway databases

BioCycARA:AT2G20050-MONOMER.
ARA:GQT-1126-MONOMER.

Gene expression databases

ArrayExpressQ9SL76.
GenevestigatorQ9SL76.

Family and domain databases

Gene3D2.60.120.10. 2 hits.
3.60.40.10. 1 hit.
InterProIPR002373. cAMP/cGMP_kin.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR000719. Prot_kinase_dom.
IPR015655. Protein_Pase_2C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
PRINTSPR00103. CAMPKINASE.
SMARTSM00100. cNMP. 2 hits.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 2 hits.
PROSITEPS50042. CNMP_BINDING_3. 2 hits.
PS01032. PP2C. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP2C19_ARATH
AccessionPrimary (citable) accession number: Q9SL76
Secondary accession number(s): B5KQ16 expand/collapse secondary AC list , B5KQ17, Q0WLT6, Q9SL77
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: April 16, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names