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Q9SL76

- P2C19_ARATH

UniProt

Q9SL76 - P2C19_ARATH

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Protein

Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein

Gene
At2g20050/At2g20040, T2G17.15/T2G17.16
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi148 – 1481Manganese 1 By similarity
Metal bindingi148 – 1481Manganese 2 By similarity
Metal bindingi149 – 1491Manganese 1; via carbonyl oxygen By similarity
Metal bindingi344 – 3441Manganese 2 By similarity
Metal bindingi388 – 3881Manganese 2 By similarity
Binding sitei811 – 8111ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi491 – 616126cNMP 1Add
BLAST
Nucleotide bindingi617 – 758142cNMP 2Add
BLAST
Nucleotide bindingi791 – 7999ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cAMP-dependent protein kinase regulator activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
  5. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. protein dephosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G20050-MONOMER.
ARA:GQT-1126-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein
Including the following 2 domains:
Probable protein phosphatase 2C 19 (EC:3.1.3.16)
Short name:
AtPP2C19
Probable inactive cyclic nucleotide-dependent protein kinase At2g20050
Gene namesi
Ordered Locus Names:At2g20050/At2g20040
ORF Names:T2G17.15/T2G17.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G20050.

Subcellular locationi

GO - Cellular componenti

  1. cAMP-dependent protein kinase complex Source: InterPro
  2. plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10941094Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing proteinPRO_0000367949Add
BLAST

Proteomic databases

PaxDbiQ9SL76.
PRIDEiQ9SL76.

Expressioni

Gene expression databases

ArrayExpressiQ9SL76.
GenevestigatoriQ9SL76.

Structurei

3D structure databases

ProteinModelPortaliQ9SL76.
SMRiQ9SL76. Positions 123-394, 472-756, 777-1093.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini136 – 378243PP2C-likeAdd
BLAST
Domaini785 – 1038254Protein kinaseAdd
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

In the N-terminal section; belongs to the PP2C family.
In the C-terminal section; belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 PP2C-like domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000030238.
InParanoidiQ0WLT6.
OMAiPAHRKTW.
PhylomeDBiQ9SL76.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
3.60.40.10. 1 hit.
InterProiIPR002373. cAMP/cGMP_kin.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR000719. Prot_kinase_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
PRINTSiPR00103. CAMPKINASE.
SMARTiSM00100. cNMP. 2 hits.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 2 hits.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS01032. PP2C. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SL76-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGCAYSKTCI GQICATKENS IRQTHQQAPS RGGTRATAAA AAVEEDNPVF     50
NFSSDAVDDV DNDEIHQLGL SRDQEWGITR LSRVSSQFLP PDGSRVVKVP 100
SCNYELRCSF LSQRGYYPDA LDKANQDSFA IHTPFGSNSD DHFFGVFDGH 150
GEFGAQCSQF VKRRLCENLL RHGRFRVDPA EACNSAFLTT NSQLHADLVD 200
DSMSGTTAIT VMVRGRTIYV ANAGDSRAVL AEKRDGDLVA VDLSIDQTPF 250
RPDELERVKL CGARVLTLDQ IEGLKNPDVQ CWGTEEDDDG DPPRLWVPNG 300
MYPGTAFTRS IGDSIAETIG VVANPEIAVV ELTPDNPFFV VASDGVFEFI 350
SSQTVVDMVA KHKDPRDACA AIVAESYRLW LQYETRTDDI TIIVVHIDGL 400
KDDAPRQLSS TGTQLQPPIP QVVELTGSES PSTFGWNSKN QRVRHDLSRA 450
RIRAIENSLE NGHAWVPPSP AHRKTWEEEA HIERVLRDHF LFRKLTDSQC 500
QVLLDCMQRL EANPGDIVVK QGGEGDCFYV VGSGEFEVLA TQDGKNGEVP 550
RILQRYTAEK QSSFGELALM HNKPLQASVR AVDHGTLWAL KREDFRGILM 600
SEFSNLASLK LLRSVDLLSR LTILQLSHVA ESLSEACFSD GQTIVTKDQK 650
LQGLYVIQKG RVKISFCTEV LESQNVSSLT TGITNEYDNL EIGTEVSIEK 700
HEGSYFGEWA LLGELKDSLS VVAVGEVVCV VLTKENFESA VGPLTNISDD 750
GPKTRHSSFE LSKESAKVTD TTALAKATLA DLEWTTCLST TDCSEIGLVH 800
LKDKENLLSL KRFSKQKVKK LGKEAQVLKE RNLMKNVIKP SAIVPEILCT 850
CVDQTFAAIL LNTTLACPIS SLLHSPLDES SVRFITGSLV SAIEDIHKNE 900
ILFRGSSPEL LMLDQSGYLQ IVDFRFAKKL SGERTFTICG NADYLAPEIV 950
QGKGHGYAAD WWALGVLIYY MLEGEMPFGS WRESELDTFQ KIAKGQLTFP 1000
RVLSSEAEDL ITKLLEVDEN LRFGSQGGPE SIKKHPWFNG LKWEAISNRE 1050
FQVPQEIISR IHHHLENDNV LPLETSKSLD TTEDQDAQNW LEEW 1094
Length:1,094
Mass (Da):121,389
Last modified:April 14, 2009 - v2
Checksum:i89C6347D846297AC
GO

Sequence cautioni

The sequence AAY27059.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAD24391.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g20040 and At2g20050.
The sequence AAD24392.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g20040 and At2g20050.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU101468 mRNA. Translation: ABU68673.1.
EU101469 mRNA. Translation: ABU68674.1.
EU591510 mRNA. Translation: ACF05481.1.
AC006081 Genomic DNA. Translation: AAD24391.1. Sequence problems.
AC006081 Genomic DNA. Translation: AAD24392.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06958.1.
AK230102 mRNA. Translation: BAF01921.1.
BT022072 mRNA. Translation: AAY27059.1. Different initiation.
BT023477 mRNA. Translation: AAY57316.1.
PIRiD84584.
RefSeqiNP_179595.5. NM_127563.5. [Q9SL76-1]
UniGeneiAt.47727.

Genome annotation databases

EnsemblPlantsiAT2G20050.1; AT2G20050.1; AT2G20050. [Q9SL76-1]
GeneIDi816524.
KEGGiath:AT2G20050.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU101468 mRNA. Translation: ABU68673.1 .
EU101469 mRNA. Translation: ABU68674.1 .
EU591510 mRNA. Translation: ACF05481.1 .
AC006081 Genomic DNA. Translation: AAD24391.1 . Sequence problems.
AC006081 Genomic DNA. Translation: AAD24392.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC06958.1 .
AK230102 mRNA. Translation: BAF01921.1 .
BT022072 mRNA. Translation: AAY27059.1 . Different initiation.
BT023477 mRNA. Translation: AAY57316.1 .
PIRi D84584.
RefSeqi NP_179595.5. NM_127563.5. [Q9SL76-1 ]
UniGenei At.47727.

3D structure databases

ProteinModelPortali Q9SL76.
SMRi Q9SL76. Positions 123-394, 472-756, 777-1093.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9SL76.
PRIDEi Q9SL76.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G20050.1 ; AT2G20050.1 ; AT2G20050 . [Q9SL76-1 ]
GeneIDi 816524.
KEGGi ath:AT2G20050.

Organism-specific databases

TAIRi AT2G20050.

Phylogenomic databases

eggNOGi COG0664.
HOGENOMi HOG000030238.
InParanoidi Q0WLT6.
OMAi PAHRKTW.
PhylomeDBi Q9SL76.

Enzyme and pathway databases

BioCyci ARA:AT2G20050-MONOMER.
ARA:GQT-1126-MONOMER.

Gene expression databases

ArrayExpressi Q9SL76.
Genevestigatori Q9SL76.

Family and domain databases

Gene3Di 2.60.120.10. 2 hits.
3.60.40.10. 1 hit.
InterProi IPR002373. cAMP/cGMP_kin.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR000719. Prot_kinase_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view ]
PRINTSi PR00103. CAMPKINASE.
SMARTi SM00100. cNMP. 2 hits.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 2 hits.
PROSITEi PS50042. CNMP_BINDING_3. 2 hits.
PS01032. PP2C. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Involvement of a putative cyclic nucleotide dependent hybrid phosphatase 2C/protein kinase gene in stress responses in higher plants."
    Van Ingelgem C., Azmi A., Inze D., Van Onckelen H., Roef L.
    Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Plants have cyclic nucleotide dependent kinases."
    Pascaud F., Corratge C., Thibaud J.-B., Lacombe B.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 424-1090.
    Strain: cv. Columbia.
  6. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 743-1090.
    Strain: cv. Columbia.
  7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C19_ARATH
AccessioniPrimary (citable) accession number: Q9SL76
Secondary accession number(s): B5KQ16
, B5KQ17, Q0WLT6, Q9SL77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: June 11, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi