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Q9SL76

- P2C19_ARATH

UniProt

Q9SL76 - P2C19_ARATH

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Protein

Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein

Gene

At2g20050/At2g20040

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi148 – 1481Manganese 1By similarity
Metal bindingi148 – 1481Manganese 2By similarity
Metal bindingi149 – 1491Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi344 – 3441Manganese 2By similarity
Metal bindingi388 – 3881Manganese 2By similarity
Binding sitei811 – 8111ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi491 – 616126cNMP 1Add
BLAST
Nucleotide bindingi617 – 758142cNMP 2Add
BLAST
Nucleotide bindingi791 – 7999ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cAMP-dependent protein kinase regulator activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
  5. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. protein dephosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G20050-MONOMER.
ARA:GQT-1126-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein
Including the following 2 domains:
Probable protein phosphatase 2C 19 (EC:3.1.3.16)
Short name:
AtPP2C19
Probable inactive cyclic nucleotide-dependent protein kinase At2g20050
Gene namesi
Ordered Locus Names:At2g20050/At2g20040
ORF Names:T2G17.15/T2G17.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G20050.

Subcellular locationi

GO - Cellular componenti

  1. cAMP-dependent protein kinase complex Source: InterPro
  2. plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10941094Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing proteinPRO_0000367949Add
BLAST

Proteomic databases

PaxDbiQ9SL76.
PRIDEiQ9SL76.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SL76. baseline and differential.
GenevestigatoriQ9SL76.

Structurei

3D structure databases

ProteinModelPortaliQ9SL76.
SMRiQ9SL76. Positions 123-394, 472-756, 777-1082.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini136 – 378243PP2C-likeAdd
BLAST
Domaini785 – 1038254Protein kinasePROSITE-ProRule annotationAdd
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

In the N-terminal section; belongs to the PP2C family.Curated
In the C-terminal section; belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.Curated
Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 PP2C-like domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000030238.
InParanoidiQ9SL76.
OMAiPAHRKTW.
PhylomeDBiQ9SL76.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
3.60.40.10. 1 hit.
InterProiIPR002373. cAMP/cGMP_kin.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR000719. Prot_kinase_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
PRINTSiPR00103. CAMPKINASE.
SMARTiSM00100. cNMP. 2 hits.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 2 hits.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS01032. PP2C. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SL76-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCAYSKTCI GQICATKENS IRQTHQQAPS RGGTRATAAA AAVEEDNPVF
60 70 80 90 100
NFSSDAVDDV DNDEIHQLGL SRDQEWGITR LSRVSSQFLP PDGSRVVKVP
110 120 130 140 150
SCNYELRCSF LSQRGYYPDA LDKANQDSFA IHTPFGSNSD DHFFGVFDGH
160 170 180 190 200
GEFGAQCSQF VKRRLCENLL RHGRFRVDPA EACNSAFLTT NSQLHADLVD
210 220 230 240 250
DSMSGTTAIT VMVRGRTIYV ANAGDSRAVL AEKRDGDLVA VDLSIDQTPF
260 270 280 290 300
RPDELERVKL CGARVLTLDQ IEGLKNPDVQ CWGTEEDDDG DPPRLWVPNG
310 320 330 340 350
MYPGTAFTRS IGDSIAETIG VVANPEIAVV ELTPDNPFFV VASDGVFEFI
360 370 380 390 400
SSQTVVDMVA KHKDPRDACA AIVAESYRLW LQYETRTDDI TIIVVHIDGL
410 420 430 440 450
KDDAPRQLSS TGTQLQPPIP QVVELTGSES PSTFGWNSKN QRVRHDLSRA
460 470 480 490 500
RIRAIENSLE NGHAWVPPSP AHRKTWEEEA HIERVLRDHF LFRKLTDSQC
510 520 530 540 550
QVLLDCMQRL EANPGDIVVK QGGEGDCFYV VGSGEFEVLA TQDGKNGEVP
560 570 580 590 600
RILQRYTAEK QSSFGELALM HNKPLQASVR AVDHGTLWAL KREDFRGILM
610 620 630 640 650
SEFSNLASLK LLRSVDLLSR LTILQLSHVA ESLSEACFSD GQTIVTKDQK
660 670 680 690 700
LQGLYVIQKG RVKISFCTEV LESQNVSSLT TGITNEYDNL EIGTEVSIEK
710 720 730 740 750
HEGSYFGEWA LLGELKDSLS VVAVGEVVCV VLTKENFESA VGPLTNISDD
760 770 780 790 800
GPKTRHSSFE LSKESAKVTD TTALAKATLA DLEWTTCLST TDCSEIGLVH
810 820 830 840 850
LKDKENLLSL KRFSKQKVKK LGKEAQVLKE RNLMKNVIKP SAIVPEILCT
860 870 880 890 900
CVDQTFAAIL LNTTLACPIS SLLHSPLDES SVRFITGSLV SAIEDIHKNE
910 920 930 940 950
ILFRGSSPEL LMLDQSGYLQ IVDFRFAKKL SGERTFTICG NADYLAPEIV
960 970 980 990 1000
QGKGHGYAAD WWALGVLIYY MLEGEMPFGS WRESELDTFQ KIAKGQLTFP
1010 1020 1030 1040 1050
RVLSSEAEDL ITKLLEVDEN LRFGSQGGPE SIKKHPWFNG LKWEAISNRE
1060 1070 1080 1090
FQVPQEIISR IHHHLENDNV LPLETSKSLD TTEDQDAQNW LEEW
Length:1,094
Mass (Da):121,389
Last modified:April 14, 2009 - v2
Checksum:i89C6347D846297AC
GO

Sequence cautioni

The sequence AAD24391.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g20040 and At2g20050.Curated
The sequence AAD24392.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At2g20040 and At2g20050.Curated
The sequence AAY27059.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU101468 mRNA. Translation: ABU68673.1.
EU101469 mRNA. Translation: ABU68674.1.
EU591510 mRNA. Translation: ACF05481.1.
AC006081 Genomic DNA. Translation: AAD24391.1. Sequence problems.
AC006081 Genomic DNA. Translation: AAD24392.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06958.1.
AK230102 mRNA. Translation: BAF01921.1.
BT022072 mRNA. Translation: AAY27059.1. Different initiation.
BT023477 mRNA. Translation: AAY57316.1.
PIRiD84584.
RefSeqiNP_179595.5. NM_127563.5. [Q9SL76-1]
UniGeneiAt.47727.

Genome annotation databases

EnsemblPlantsiAT2G20050.1; AT2G20050.1; AT2G20050. [Q9SL76-1]
GeneIDi816524.
KEGGiath:AT2G20050.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU101468 mRNA. Translation: ABU68673.1 .
EU101469 mRNA. Translation: ABU68674.1 .
EU591510 mRNA. Translation: ACF05481.1 .
AC006081 Genomic DNA. Translation: AAD24391.1 . Sequence problems.
AC006081 Genomic DNA. Translation: AAD24392.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC06958.1 .
AK230102 mRNA. Translation: BAF01921.1 .
BT022072 mRNA. Translation: AAY27059.1 . Different initiation.
BT023477 mRNA. Translation: AAY57316.1 .
PIRi D84584.
RefSeqi NP_179595.5. NM_127563.5. [Q9SL76-1 ]
UniGenei At.47727.

3D structure databases

ProteinModelPortali Q9SL76.
SMRi Q9SL76. Positions 123-394, 472-756, 777-1082.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9SL76.
PRIDEi Q9SL76.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G20050.1 ; AT2G20050.1 ; AT2G20050 . [Q9SL76-1 ]
GeneIDi 816524.
KEGGi ath:AT2G20050.

Organism-specific databases

TAIRi AT2G20050.

Phylogenomic databases

eggNOGi COG0664.
HOGENOMi HOG000030238.
InParanoidi Q9SL76.
OMAi PAHRKTW.
PhylomeDBi Q9SL76.

Enzyme and pathway databases

BioCyci ARA:AT2G20050-MONOMER.
ARA:GQT-1126-MONOMER.

Gene expression databases

ExpressionAtlasi Q9SL76. baseline and differential.
Genevestigatori Q9SL76.

Family and domain databases

Gene3Di 2.60.120.10. 2 hits.
3.60.40.10. 1 hit.
InterProi IPR002373. cAMP/cGMP_kin.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR000719. Prot_kinase_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view ]
PRINTSi PR00103. CAMPKINASE.
SMARTi SM00100. cNMP. 2 hits.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 2 hits.
PROSITEi PS50042. CNMP_BINDING_3. 2 hits.
PS01032. PP2C. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Involvement of a putative cyclic nucleotide dependent hybrid phosphatase 2C/protein kinase gene in stress responses in higher plants."
    Van Ingelgem C., Azmi A., Inze D., Van Onckelen H., Roef L.
    Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Plants have cyclic nucleotide dependent kinases."
    Pascaud F., Corratge C., Thibaud J.-B., Lacombe B.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 424-1090.
    Strain: cv. Columbia.
  6. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 743-1090.
    Strain: cv. Columbia.
  7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C19_ARATH
AccessioniPrimary (citable) accession number: Q9SL76
Secondary accession number(s): B5KQ16
, B5KQ17, Q0WLT6, Q9SL77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: November 26, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3