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Reviewed, UniProtKB/Swiss-Prot Q9SL03 (CALS2_ARATH)

Last modified June 16, 2009. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Callose synthase 2
    EC=2.4.1.34
Alternative name(s):
    1,3-beta-glucan synthase
    Protein GLUCAN SYNTHASE-LIKE 3
Gene names
Name: CALS2
Synonyms: GSL3
Ordered Locus Names: At2g31960
ORF Names: F22D22.29
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length1951 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Involved in callose synthesis at the forming cell plate during cytokinesis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals By similarity.

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Sequence caution

The sequence AAD15408.1 differs from that shown. Reason: Erroneous gene model prediction.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19511951Callose synthase 2
PRO_0000334574

Regions

Topological domain1 – 481481Cytoplasmic Potential
Transmembrane482 – 50221 Potential
Topological domain503 – 5053Extracellular Potential
Transmembrane506 – 52621 Potential
Topological domain527 – 55226Cytoplasmic Potential
Transmembrane553 – 57321 Potential
Topological domain574 – 59825Extracellular Potential
Transmembrane599 – 61921 Potential
Topological domain620 – 65536Cytoplasmic Potential
Transmembrane656 – 67621 Potential
Topological domain677 – 71438Extracellular Potential
Transmembrane715 – 73521 Potential
Topological domain736 – 1512777Cytoplasmic Potential
Transmembrane1513 – 153321 Potential
Topological domain1534 – 156128Extracellular Potential
Transmembrane1562 – 158221 Potential
Topological domain1583 – 159210Cytoplasmic Potential
Transmembrane1593 – 161321 Potential
Topological domain1614 – 165643Extracellular Potential
Transmembrane1657 – 167721 Potential
Topological domain1678 – 16836Cytoplasmic Potential
Transmembrane1684 – 170421 Potential
Topological domain1705 – 175652Extracellular Potential
Transmembrane1757 – 177721 Potential
Topological domain1778 – 178811Cytoplasmic Potential
Transmembrane1789 – 180921 Potential
Topological domain1810 – 182819Extracellular Potential
Transmembrane1829 – 184921 Potential
Topological domain1850 – 18523Cytoplasmic Potential
Transmembrane1853 – 187321 Potential
Topological domain1874 – 189522Extracellular Potential
Transmembrane1896 – 191621 Potential
Topological domain1917 – 195135Cytoplasmic Potential
Compositional bias7 – 126Poly-Pro

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Sequences

Sequence LengthMass (Da)Tools
Q9SL03-1 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: A0600B39B390DB56

FASTA1,951226,148
        10         20         30         40         50         60 
MAQRKGPDPP PPQRRILRTQ TAGNLGEAML DSEVVPSSLV EIAPILRVAN EVEASNPRVA 

        70         80         90        100        110        120 
YLCRFYAFEK AHRLDPTSSG RGVRQFKTAL LQRLERENET TLAGRQKSDA REMQSFYQHY 

       130        140        150        160        170        180 
YKKYIQALQN AADKADRAQL TKAYQTAAVL FEVLKAVNQT EDVEVADEIL EAHTKVEEKS 

       190        200        210        220        230        240 
QIYVPYNILP LDPDSQNQAI MRFPEIQATV SALRNTRGLP WPAGHKKKLD EDMLDWLQTM 

       250        260        270        280        290        300 
FGFQKDNVSN QREHLILLLA NVHIRQFPRP EQQPRLDDRA LTIVMKKLFK NYKKWCKYLG 

       310        320        330        340        350        360 
RKSSLWLPTI QQEVQQRKLL YMGLYLLIWG EAANLRFLPE CLCYIYHHMA FELYGMLAGS 

       370        380        390        400        410        420 
VSPMTGEHVK PAYGGEDEAF LQKVVTPIYK TIAKEAKRSR GGKSKHSEWR NYDDLNEYFW 

       430        440        450        460        470        480 
SIRCFRLGWP MRADADFFCQ TAEELRLDRS ENKPKTGDRW MGKVNFVEIR SFWHIFRSFD 

       490        500        510        520        530        540 
RMWSFYILSL QAMIIIAWNG SGKLSGIFQG DVFLKVLSIF ITAAILKLAQ AVLDIALSWK 

       550        560        570        580        590        600 
SRHSMSFHVK LRFIFKAVAA AIWVVLMPLT YAYSWKTPSG FAETIKNWFG GHQNSSPSFF 

       610        620        630        640        650        660 
IIVILIYLSP NMLSTLLFAF PFIRRYLERS DYKIVMLMMW WSQPRLYIGR GMHESALSLF 

       670        680        690        700        710        720 
KYTMFWVVLL ISKLAFSFYA EQIKPLVKPT KDIMRVHISV YRWHEFFPHA KSNMGVVIAL 

       730        740        750        760        770        780 
WSPVILVYFM DTQIWYAIVS TLVGGLNGAF RRLGEIRTLG MLRSRFQSLP EAFNACLVPN 

       790        800        810        820        830        840 
EKSETPKKKG IMATFTRKFD QVPSSKDKEA ARFAQMWNKI ISSFREEDLI SDREMELLLV 

       850        860        870        880        890        900 
PYWADRDLDL IRWPPFLLAS KIPIALDMAK DSNGKDRELT KRLSVDSYMT CAVRECYASF 

       910        920        930        940        950        960 
KNLINFLVVG EREGQVINEI FSRIDEHIEK ETLIKDLNLS ALPDLYGQFV RLIEYLMENR 

       970        980        990       1000       1010       1020 
EEDKDQIVIV LLNMLEVVTR DIMDEEVPSM LESTHNGTYV KYDVMTPLHQ QRKYFSQLRF 

      1030       1040       1050       1060       1070       1080 
PVYSQTEAWK EKIKRLHLLL TVKESAMDVP SNLEARRRLT FFSNSLFMEM PDAPKIRNML 

      1090       1100       1110       1120       1130       1140 
SFSVLTPYYS EDVLFSIFGL EKQNEDGVSI LFYLQKIFPD EWTNFLERVK CGSEEELRAR 

      1150       1160       1170       1180       1190       1200 
EELEEELRLW ASYRGQTLTK TVRGMMYYRK ALELQAFLDM AKDEELMKGY KALELTSEDA 

      1210       1220       1230       1240       1250       1260 
SKSGTSLWAQ CQALADMKFT FVVSCQQYSV QKRSGDQRAK DILRLMTTYP SLRVAYIDEV 

      1270       1280       1290       1300       1310       1320 
EQTHKESYKG ADEKIYYSAL VKAAPQTKSM DSSESVQTLD QVIYRIKLPG PAILGEGKPE 

      1330       1340       1350       1360       1370       1380 
NQNHSIIFTR GEGLQTIDMN QDNYMEEAFK MRNLLQEFLV KHGGVRTPTI LGLREHIFTG 

      1390       1400       1410       1420       1430       1440 
SVSSLAWFMS NQENSFVTIG QRVLASPLKV RFHYGHPDVF DRLFHLTRGG VCKASKVINL 

      1450       1460       1470       1480       1490       1500 
SEDIFAGFNS TLREGNVTHH EYIQVGKGRD VGLNQISMFE AKIANGNGEQ TLSRDLYRLG 

      1510       1520       1530       1540       1550       1560 
HRFDFFRMLS CYFTTIGFYF STMLTVLTVY VFLYGRLYLV LSGLEEGLSN QKAFRSNMPL 

      1570       1580       1590       1600       1610       1620 
QAALASQSFV QIGFLMALPM MMEIGLERGF HNALIDFVLM QLQLASVFFT FQLGTKTHYY 

      1630       1640       1650       1660       1670       1680 
GRTLFHGGAE YRGTGRGFVV FHAKFAENYR FYSRSHFVKG IELMILLLVY QIFGHAYRGV 

      1690       1700       1710       1720       1730       1740 
VTYILITVSI WFMVVTWLFA PFLFNPSGFE WQKIVDDWTD WNKWIYNRGG IGVPPEKSWE 

      1750       1760       1770       1780       1790       1800 
SWWEKEIGHL RHSGKRGIIL EIVLALRFFI FQYGLVYQLS TFKQENQSLW IYGASWFVIL 

      1810       1820       1830       1840       1850       1860 
FILLIVKGLG VGRQRFSTNF QLLFRIIKGF VFLTFLGLLI TFLALRFLTP KDIFLCMLAF 

      1870       1880       1890       1900       1910       1920 
MPTGWGMLLI AQACKPLIQR LGFWSSVRTL ARGYEILMGL LLFTPVAFLA WFPFVSEFQT 

      1930       1940       1950 
RMLFNQAFSR GLQISRILGG QRKDRSSKNK E

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References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed: 11910074] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1205-1951.
Strain: cv. Columbia.
[3]"A cell plate-specific callose synthase and its interaction with phragmoplastin."
Hong Z., Delauney A.J., Verma D.P.S.
Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract]
Cited for: GENE FAMILY AND NOMENCLATURE.
[4]"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract]
Cited for: NOMENCLATURE.

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Cross-references

Sequence databases

AC006223 Genomic DNA. Translation: AAD15408.1. Sequence problems.
AK117353 mRNA. Translation: BAC42023.1. Different initiation.
IPIIPI00521194.
PIRC84727.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Proteomic databases

PRIDEQ9SL03.

Genome annotation databases

GenomeReviewsGene locus AT2G31960 in contig CT485783_GR.

Organism-specific databases

TAIRAt2g31960.

Family and domain databases

InterProIPR003440. Glyco_trans_48.
[Graphical view]
PfamPF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCALS2_ARATH
AccessionPrimary (citable) accession number: Q9SL03
Secondary accession number(s): Q8GYW2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: June 16, 2009
This is version 34 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents