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Q9SL03 (CALS2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Callose synthase 2
EC=2.4.1.34
Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 3
Gene names
Name:CALS2
Synonyms:GSL3
Ordered Locus Names:At2g31960
ORF Names:F22D22.29
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length1950 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in callose synthesis at the forming cell plate during cytokinesis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals By similarity.

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Sequence caution

The sequence AAD15408.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAC42023.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19501950Callose synthase 2
PRO_0000334574

Regions

Topological domain1 – 481481Cytoplasmic Potential
Transmembrane482 – 50221Helical; Potential
Topological domain503 – 5053Extracellular Potential
Transmembrane506 – 52621Helical; Potential
Topological domain527 – 55226Cytoplasmic Potential
Transmembrane553 – 57321Helical; Potential
Topological domain574 – 59825Extracellular Potential
Transmembrane599 – 61921Helical; Potential
Topological domain620 – 65536Cytoplasmic Potential
Transmembrane656 – 67621Helical; Potential
Topological domain677 – 71337Extracellular Potential
Transmembrane714 – 73421Helical; Potential
Topological domain735 – 1511777Cytoplasmic Potential
Transmembrane1512 – 153221Helical; Potential
Topological domain1533 – 156028Extracellular Potential
Transmembrane1561 – 158121Helical; Potential
Topological domain1582 – 159110Cytoplasmic Potential
Transmembrane1592 – 161221Helical; Potential
Topological domain1613 – 165543Extracellular Potential
Transmembrane1656 – 167621Helical; Potential
Topological domain1677 – 16826Cytoplasmic Potential
Transmembrane1683 – 170321Helical; Potential
Topological domain1704 – 175552Extracellular Potential
Transmembrane1756 – 177621Helical; Potential
Topological domain1777 – 178711Cytoplasmic Potential
Transmembrane1788 – 180821Helical; Potential
Topological domain1809 – 182719Extracellular Potential
Transmembrane1828 – 184821Helical; Potential
Topological domain1849 – 18513Cytoplasmic Potential
Transmembrane1852 – 187221Helical; Potential
Topological domain1873 – 189422Extracellular Potential
Transmembrane1895 – 191521Helical; Potential
Topological domain1916 – 195035Cytoplasmic Potential
Compositional bias7 – 126Poly-Pro

Sequences

Sequence LengthMass (Da)Tools
Q9SL03 [UniParc].

Last modified November 16, 2011. Version 3.
Checksum: 29E48783AF58EB26

FASTA1,950226,019
        10         20         30         40         50         60 
MAQRKGPDPP PPQRRILRTQ TAGNLGEAML DSEVVPSSLV EIAPILRVAN EVEASNPRVA 

        70         80         90        100        110        120 
YLCRFYAFEK AHRLDPTSSG RGVRQFKTAL LQRLERENET TLAGRQKSDA REMQSFYQHY 

       130        140        150        160        170        180 
YKKYIQALQN AADKADRAQL TKAYQTAAVL FEVLKAVNQT EDVEVADEIL EAHTKVEEKS 

       190        200        210        220        230        240 
QIYVPYNILP LDPDSQNQAI MRFPEIQATV SALRNTRGLP WPAGHKKKLD EDMLDWLQTM 

       250        260        270        280        290        300 
FGFQKDNVSN QREHLILLLA NVHIRQFPRP EQQPRLDDRA LTIVMKKLFK NYKKWCKYLG 

       310        320        330        340        350        360 
RKSSLWLPTI QQEVQQRKLL YMGLYLLIWG EAANLRFLPE CLCYIYHHMA FELYGMLAGS 

       370        380        390        400        410        420 
VSPMTGEHVK PAYGGEDEAF LQKVVTPIYK TIAKEAKRSR GGKSKHSEWR NYDDLNEYFW 

       430        440        450        460        470        480 
SIRCFRLGWP MRADADFFCQ TAEELRLDRS ENKPKTGDRW MGKVNFVEIR SFWHIFRSFD 

       490        500        510        520        530        540 
RMWSFYILSL QAMIIIAWNG SGKLSGIFQG DVFLKVLSIF ITAAILKLAQ AVLDIALSWK 

       550        560        570        580        590        600 
SRHSMSFHVK LRFIFKAVAA AIWVVLMPLT YAYSWKTPSG FAETIKNWFG GHQNSSPSFF 

       610        620        630        640        650        660 
IIVILIYLSP NMLSTLLFAF PFIRRYLERS DYKIVMLMMW WSQPRLYIGR GMHESALSLF 

       670        680        690        700        710        720 
KYTMFWVVLL ISKLAFSFYA EIKPLVKPTK DIMRVHISVY RWHEFFPHAK SNMGVVIALW 

       730        740        750        760        770        780 
SPVILVYFMD TQIWYAIVST LVGGLNGAFR RLGEIRTLGM LRSRFQSLPE AFNACLVPNE 

       790        800        810        820        830        840 
KSETPKKKGI MATFTRKFDQ VPSSKDKEAA RFAQMWNKII SSFREEDLIS DREMELLLVP 

       850        860        870        880        890        900 
YWADRDLDLI RWPPFLLASK IPIALDMAKD SNGKDRELTK RLSVDSYMTC AVRECYASFK 

       910        920        930        940        950        960 
NLINFLVVGE REGQVINEIF SRIDEHIEKE TLIKDLNLSA LPDLYGQFVR LIEYLMENRE 

       970        980        990       1000       1010       1020 
EDKDQIVIVL LNMLEVVTRD IMDEEVPSML ESTHNGTYVK YDVMTPLHQQ RKYFSQLRFP 

      1030       1040       1050       1060       1070       1080 
VYSQTEAWKE KIKRLHLLLT VKESAMDVPS NLEARRRLTF FSNSLFMEMP DAPKIRNMLS 

      1090       1100       1110       1120       1130       1140 
FSVLTPYYSE DVLFSIFGLE KQNEDGVSIL FYLQKIFPDE WTNFLERVKC GSEEELRARE 

      1150       1160       1170       1180       1190       1200 
ELEEELRLWA SYRGQTLTKT VRGMMYYRKA LELQAFLDMA KDEELMKGYK ALELTSEDAS 

      1210       1220       1230       1240       1250       1260 
KSGTSLWAQC QALADMKFTF VVSCQQYSVQ KRSGDQRAKD ILRLMTTYPS LRVAYIDEVE 

      1270       1280       1290       1300       1310       1320 
QTHKESYKGA DEKIYYSALV KAAPQTKSMD SSESVQTLDQ VIYRIKLPGP AILGEGKPEN 

      1330       1340       1350       1360       1370       1380 
QNHSIIFTRG EGLQTIDMNQ DNYMEEAFKM RNLLQEFLVK HGGVRTPTIL GLREHIFTGS 

      1390       1400       1410       1420       1430       1440 
VSSLAWFMSN QENSFVTIGQ RVLASPLKVR FHYGHPDVFD RLFHLTRGGV CKASKVINLS 

      1450       1460       1470       1480       1490       1500 
EDIFAGFNST LREGNVTHHE YIQVGKGRDV GLNQISMFEA KIANGNGEQT LSRDLYRLGH 

      1510       1520       1530       1540       1550       1560 
RFDFFRMLSC YFTTIGFYFS TMLTVLTVYV FLYGRLYLVL SGLEEGLSNQ KAFRSNMPLQ 

      1570       1580       1590       1600       1610       1620 
AALASQSFVQ IGFLMALPMM MEIGLERGFH NALIDFVLMQ LQLASVFFTF QLGTKTHYYG 

      1630       1640       1650       1660       1670       1680 
RTLFHGGAEY RGTGRGFVVF HAKFAENYRF YSRSHFVKGI ELMILLLVYQ IFGHAYRGVV 

      1690       1700       1710       1720       1730       1740 
TYILITVSIW FMVVTWLFAP FLFNPSGFEW QKIVDDWTDW NKWIYNRGGI GVPPEKSWES 

      1750       1760       1770       1780       1790       1800 
WWEKEIGHLR HSGKRGIILE IVLALRFFIF QYGLVYQLST FKQENQSLWI YGASWFVILF 

      1810       1820       1830       1840       1850       1860 
ILLIVKGLGV GRQRFSTNFQ LLFRIIKGFV FLTFLGLLIT FLALRFLTPK DIFLCMLAFM 

      1870       1880       1890       1900       1910       1920 
PTGWGMLLIA QACKPLIQRL GFWSSVRTLA RGYEILMGLL LFTPVAFLAW FPFVSEFQTR 

      1930       1940       1950 
MLFNQAFSRG LQISRILGGQ RKDRSSKNKE

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed: 11910074] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1204-1950.
Strain: cv. Columbia.
[4]"A cell plate-specific callose synthase and its interaction with phragmoplastin."
Hong Z., Delauney A.J., Verma D.P.S.
Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract]
Cited for: GENE FAMILY AND NOMENCLATURE.
[5]"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC006223 Genomic DNA. Translation: AAD15408.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08612.1.
CP002685 Genomic DNA. Translation: AEC08613.1.
AK117353 mRNA. Translation: BAC42023.1. Different initiation.
IPIIPI01020376.
PIRC84727.
RefSeqNP_001189653.1. NM_001202724.1.
NP_850178.2. NM_179847.2.
UniGeneAt.38137.

3D structure databases

ProteinModelPortalQ9SL03.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9SL03.

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Proteomic databases

PRIDEQ9SL03.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID817755.
GenomeReviewsGene locus AT2G31960 in contig CT485783_GR.

Organism-specific databases

TAIRAt2g31960.

Phylogenomic databases

eggNOGKOG0916.
GeneTreeEPGT00070000028111.
HOGENOMHBG316390.
PhylomeDBQ9SL03.

Gene expression databases

GenevestigatorQ9SL03.

Family and domain databases

InterProIPR006745. DUF605.
IPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1_N.
[Graphical view]
Gene3DG3DSA:1.25.40.270. G3DSA:1.25.40.270. 1 hit.
PfamPF04652. DUF605. 1 hit.
PF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCALS2_ARATH
AccessionPrimary (citable) accession number: Q9SL03
Secondary accession number(s): F4IRV4, Q8GYW2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 16, 2011
Last modified: January 25, 2012
This is version 52 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents