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Q9SKX2 (PME16_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable pectinesterase/pectinesterase inhibitor 16

Including the following 2 domains:

  1. Pectinesterase inhibitor 16
    Alternative name(s):
    Pectin methylesterase inhibitor 16
  2. Pectinesterase 16
    Short name=PE 16
    EC=3.1.1.11
    Alternative name(s):
    AtPMEpcrD
    Pectin methylesterase 16
    Short name=AtPME16
Gene names
Name:PME16
Synonyms:ARATH16
Ordered Locus Names:At2g43050
ORF Names:MFL8.9
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length518 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.

Catalytic activity

Pectin + n H2O = n methanol + pectate.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.

Subcellular location

Secreted › cell wall By similarity.

Tissue specificity

Expressed in siliques and floral stems. Ref.4

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Sequence similarities

In the N-terminal section; belongs to the PMEI family.

In the C-terminal section; belongs to the pectinesterase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 518485Probable pectinesterase/pectinesterase inhibitor 16
PRO_0000371673

Regions

Region30 – 172143Pectinesterase inhibitor 16
Region213 – 502290Pectinesterase 16
Compositional bias34 – 5623Ser-rich

Sites

Active site3421Proton donor; for pectinesterase activity By similarity
Active site3631Nucleophile; for pectinesterase activity By similarity
Binding site2891Substrate; for pectinesterase activity By similarity
Binding site3191Substrate; for pectinesterase activity By similarity
Binding site4221Substrate; for pectinesterase activity By similarity
Binding site4241Substrate; for pectinesterase activity By similarity
Site3411Transition state stabilizer By similarity

Amino acid modifications

Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation1611N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9SKX2 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: C5E7A903F0786604

FASTA51856,589
        10         20         30         40         50         60 
MASSSSISNH KIPNTLMFLV IVNFLYLIQT NSAVSISSNS NSHFSRFSRH RSSPSSKTKQ 

        70         80         90        100        110        120 
GFLATVQESM NHALLARSLA FNLTLSHRTV QTHTFDPIHD CLELLDDTLD MLSRIHADND 

       130        140        150        160        170        180 
EEDVHTWLSA ALTNQDTCEQ SLQEKSESYK HGLAMDFVAR NLTGLLTSSL DLFVSVKSKH 

       190        200        210        220        230        240 
RKLLSKQEYF PTFVPSSEQR RLLEAPVEEL NVDAVVAPDG SGTHKTIGEA LLSTSLASSG 

       250        260        270        280        290        300 
GRTKIYLKAG TYHENINIPT KQKNVMLVGD GKGKTVIVGS RSNRGGWTTY KTATVAAMGE 

       310        320        330        340        350        360 
GFIARDMTFV NNAGPKSEQA VALRVGADKS VVHRCSVEGY QDSLYTHSKR QFYRETDITG 

       370        380        390        400        410        420 
TVDFIFGNSA VVFQSCNIAA RKPLPGQRNF VTAQGRSNPG QNTGIAIQNC RITAESMTYL 

       430        440        450        460        470        480 
GRPWKEYSRT VVMQSFIGGS IHPSGWSPWS GGFGLKSLFY GEYGNSGPGS SVSGRVKWSG 

       490        500        510 
CHPSLTVTEA EKFTVASFID GNIWLPSTGV SFDPGLVN

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Arabidopsis ORF clones."
Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Characterization of the pectin methylesterase-like gene AtPME3: a new member of a gene family comprising at least 12 genes in Arabidopsis thaliana."
Micheli F., Holliger C., Goldberg R., Richard L.
Gene 220:13-20(1998) [PubMed: 9767082] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Pectin methylesterases: sequence-structural features and phylogenetic relationships."
Markovic O., Janecek S.
Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC006224 Genomic DNA. Translation: AAD22126.1.
CP002685 Genomic DNA. Translation: AEC10203.1.
BT023433 mRNA. Translation: AAY56424.1.
IPIIPI00533491.
PIRD84861.
RefSeqNP_181833.1. NM_129866.4.
UniGeneAt.43649.
At.67744.
At.74784.
At.74860.

3D structure databases

HSSPHSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ProteinModelPortalQ9SKX2.
SMRQ9SKX2. Positions 210-517.
ModBaseSearch...

Proteomic databases

PRIDEQ9SKX2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G43050.1; AT2G43050.1; AT2G43050.
GeneID818907.
GenomeReviewsGene locus AT2G43050 in contig CT485783_GR.
KEGGath:AT2G43050.
NMPDRfig|3702.1.peg.11455.

Organism-specific databases

GeneFarm435. 8.
TAIRAt2g43050.

Phylogenomic databases

eggNOGCOG4677.
GeneTreeEPGT00070000027901.
HOGENOMHBG747179.
InParanoidQ9SKX2.
OMATYHENIN.
PhylomeDBQ9SKX2.
ProtClustDBPLN02170.

Gene expression databases

ArrayExpressQ9SKX2.
GenevestigatorQ9SKX2.

Family and domain databases

InterProIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR018040. Pectinesterase_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
G3DSA:1.20.140.40. Pectinesterase_inhib. 1 hit.
PfamPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMSSF51126. Pectin_lyas_like. 1 hit.
SSF101148. Pectinesterase_inhib. 1 hit.
PROSITEPS00800. PECTINESTERASE_1. False negative.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePME16_ARATH
AccessionPrimary (citable) accession number: Q9SKX2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: December 14, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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