Reviewed, UniProtKB/Swiss-Prot Q9SKP6 (TPIC_ARATH)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase, chloroplastic
      Short name=TIM
      Short name=Triose-phosphate isomerase
    EC=5.3.1.1

Gene names

Name:	TIM
Ordered Locus Names:	At2g21170
ORF Names:	F26H11.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Miscellaneous	In plants, there are two types of TPIS, cytosolic and plastid.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast envelope Inferred from direct assay. Source: TAIR chloroplast stroma Inferred from direct assay. Source: TAIR mitochondrion Inferred from direct assay. Source: TAIR thylakoid Inferred from direct assay. Source: TAIR
Molecular function	protein binding Inferred from physical interaction. Source: IntAct triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449224,EBI-449157

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SKP6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 60	60	Chloroplast By similarity
	Chain	61 – 315	255	Triosephosphate isomerase, chloroplastic		PRO_0000035649
Sites
	Active site	155	1	Electrophile By similarity
	Active site	225	1	Proton acceptor By similarity
	Binding site	71	1	Substrate By similarity
	Binding site	73	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: BD580F9B37FA0269	FASTA	315	33,346
	10 20 30 40 50 60 MAATSLTAPP SFSGLRRISP KLDAAAVSSH QSFFHRVNSS TRLVSSSSSS HRSPRGVVAM 70 80 90 100 110 120 AGSGKFFVGG NWKCNGTKDS IAKLISDLNS ATLEADVDVV VSPPFVYIDQ VKSSLTDRID 130 140 150 160 170 180 ISGQNSWVGK GGAFTGEISV EQLKDLGCKW VILGHSERRH VIGEKDEFIG KKAAYALSEG 190 200 210 220 230 240 LGVIACIGEK LEEREAGKTF DVCFAQLKAF ADAVPSWDNI VVAYEPVWAI GTGKVASPQQ 250 260 270 280 290 300 AQEVHVAVRG WLKKNVSEEV ASKTRIIYGG SVNGGNSAEL AKEEDIDGFL VGGASLKGPE 310 FATIVNSVTS KKVAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and regulation of nuclear genes encoding chloroplast and cytosolic triosephosphate isomerase in Arabidopsis thaliana." Shih M.-C. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC006264 Genomic DNA. Translation: AAD29799.1. AF247559 mRNA. Translation: AAF70259.1. AF378898 mRNA. Translation: AAK55701.1. AY052748 mRNA. Translation: AAK96462.1. AY087893 mRNA. Translation: AAM65444.1.
IPI	IPI00524641.
PIR	A84598.
RefSeq	NP_179713.1.
UniGene	At.24668 At.31909
3D structure databases
HSSP	HSSP built from PDB template 1TPH based on UniProtKB P00940.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SKP6. 1 interaction.
Proteomic databases
PRIDE	Q9SKP6.
ProMEX	Q9SKP6.
Genome annotation databases
GeneID	816652.
GenomeReviews	Gene locus AT2G21170 in contig CT485783_GR.
NMPDR	fig|3702.1.peg.9136.
Organism-specific databases
TAIR	At2g21170.
Phylogenomic databases
OMA	Q9SKP6. AGSGKFF.
Enzyme and pathway databases
BRENDA	5.3.1.1. 302.
Gene expression databases
ArrayExpress	Q9SKP6.
GermOnline	AT2G21170. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIC_ARATH

Accession

Primary (citable) accession number: Q9SKP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)