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Q9SKN2 (DRB2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Double-stranded RNA-binding protein 2
Alternative name(s):
dsRNA-binding protein 2
Short name=AtDRB2
Gene names
Name:DRB2
Ordered Locus Names:At2g28380
ORF Names:T1B3.10
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds double-stranded RNA. May be involved in RNA-mediated silencing. Ref.4

Subunit structure

Heterodimer with DRB1 or DRB5. Interacts with DCL1 and DCL5. Ref.4

Subcellular location

Cytoplasm Ref.4.

Disruption phenotype

Flat, serrated, blue-green and ovoid leaves. Ref.5

Sequence similarities

Contains 2 DRBM (double-stranded RNA-binding) domains.

Ontologies

Keywords
   Biological processRNA-mediated gene silencing
   Cellular componentCytoplasm
   DomainRepeat
   LigandRNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processgene silencing by RNA

Inferred from electronic annotation. Source: UniProtKB-KW

rRNA catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from direct assay Ref.4. Source: TAIR

   Molecular functiondouble-stranded RNA binding

Inferred from direct assay Ref.4. Source: TAIR

protein binding

Inferred from physical interaction Ref.4. Source: UniProtKB

ribonuclease III activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434Double-stranded RNA-binding protein 2
PRO_0000404653

Regions

Domain1 – 7070DRBM 1
Domain87 – 15569DRBM 2
Compositional bias304 – 3107Poly-Pro

Sequences

Sequence LengthMass (Da)Tools
Q9SKN2 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 72DE2AC3C567C16C

FASTA43447,440
        10         20         30         40         50         60 
MYKNQLQELA QRSCFNLPSY TCIREGPDHA PRFKATVNFN GEIFESPQYC STLRQAEHSA 

        70         80         90        100        110        120 
AEVALNALSN RGPSHSLAAR ILDETGVYKN LLQEIAQRVG APLPRYTTFR SGLGHQPVFT 

       130        140        150        160        170        180 
GTVELAGITF TGDPAKNKKQ AEKNAAMAAW SSLKQLAKET SSSMPEPENI DELEQVIIAR 

       190        200        210        220        230        240 
ALINYRIKEN IGTGSSSSAP VPFAKKFFMQ NLRPTSPQPS PATTSRILPF ICPKQPSRSS 

       250        260        270        280        290        300 
RSSLAATSGI DRIMAAALES RSYQRPQQRF ANPPYVPMRQ FRSQCHGMAP PVTIRTAVPV 

       310        320        330        340        350        360 
FSAPPMPPPP CTNNTQLPSS VYVPSLMRTA PPVRIAPPVT IRTAVPVFAS APPVRIRTAV 

       370        380        390        400        410        420 
KPTVEAGETR ISSVQEKESI PVLPDSLEIG VEGSTITITD CEKTASKETE RAEFKDSSKG 

       430 
EPETARERLE NLKI 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana."
Hiraguri A., Itoh R., Kondo N., Nomura Y., Aizawa D., Murai Y., Koiwa H., Seki M., Shinozaki K., Fukuhara T.
Plant Mol. Biol. 57:173-188(2005) [PubMed: 15821876] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, INTERACTION WITH DCL1; DCL5; DRB1 AND DRB5.
[5]"The roles of plant dsRNA-binding proteins in RNAi-like pathways."
Curtin S.J., Watson J.M., Smith N.A., Eamens A.L., Blanchard C.L., Waterhouse P.M.
FEBS Lett. 582:2753-2760(2008) [PubMed: 18625233] [Abstract]
Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006283 Genomic DNA. Translation: AAD20688.1.
CP002685 Genomic DNA. Translation: AEC08114.1.
AY034976 mRNA. Translation: AAK59481.1.
AY062965 mRNA. Translation: AAL33811.1.
IPIIPI00547657.
PIRB84684.
RefSeqNP_565672.1. NM_128398.3.
UniGeneAt.13732.

3D structure databases

ProteinModelPortalQ9SKN2.
SMRQ9SKN2. Positions 1-70, 86-154.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9SKN2. 8 interactions.
STRINGQ9SKN2.

Proteomic databases

PRIDEQ9SKN2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G28380.1; AT2G28380.1; AT2G28380.
GeneID817386.
GenomeReviewsGene locus AT2G28380 in contig CT485783_GR.
KEGGath:AT2G28380.
NMPDRfig|3702.1.peg.9889.

Organism-specific databases

TAIRAt2g28380.

Phylogenomic databases

GeneTreeEPGT00050000001224.
HOGENOMHBG602072.
InParanoidQ9SKN2.
OMAFTGDPAK.
PhylomeDBQ9SKN2.
ProtClustDBCLSN2688512.

Gene expression databases

ArrayExpressQ9SKN2.
GenevestigatorQ9SKN2.

Family and domain databases

InterProIPR001159. Ds-RNA-bd.
IPR014720. dsRNA-bd-like.
IPR011907. RNase_III_bac.
[Graphical view]
Gene3DG3DSA:3.30.160.20. dsRNA-bd-like. 2 hits.
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 2 hits.
[Graphical view]
SMARTSM00358. DSRM. 2 hits.
[Graphical view]
PROSITEPS50137. DS_RBD. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDRB2_ARATH
AccessionPrimary (citable) accession number: Q9SKN2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: May 1, 2000
Last modified: December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families