Q9SKN2 (DRB2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Double-stranded RNA-binding protein 2

Alternative name(s):
dsRNA-binding protein 2
Short name=AtDRB2

Gene names

Name:	DRB2
Ordered Locus Names:	At2g28380
ORF Names:	T1B3.10

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds double-stranded RNA. May be involved in RNA-mediated silencing. Ref.4
Subunit structure	Heterodimer with DRB1 or DRB5. Interacts with DCL1 and DCL5. Ref.4
Subcellular location	Cytoplasm Ref.4.
Disruption phenotype	Flat, serrated, blue-green and ovoid leaves. Ref.5
Sequence similarities	Contains 2 DRBM (double-stranded RNA-binding) domains.

Ontologies

Keywords
Biological process	RNA-mediated gene silencing
Cellular component	Cytoplasm
Domain	Repeat
Ligand	RNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gene silencing by RNA Inferred from electronic annotation. Source: UniProtKB-KW rRNA catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay Ref.4. Source: TAIR
Molecular function	double-stranded RNA binding Inferred from direct assay Ref.4. Source: TAIR protein binding Inferred from physical interaction Ref.4. Source: UniProtKB ribonuclease III activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Double-stranded RNA-binding protein 2		PRO_0000404653
Regions
	Domain	1 – 70	70	DRBM 1
	Domain	87 – 155	69	DRBM 2
	Compositional bias	304 – 310	7	Poly-Pro

Sequences

	Sequence		Length	Mass (Da)
	Q9SKN2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 72DE2AC3C567C16C	FASTA	434	47,440
	10 20 30 40 50 60 MYKNQLQELA QRSCFNLPSY TCIREGPDHA PRFKATVNFN GEIFESPQYC STLRQAEHSA 70 80 90 100 110 120 AEVALNALSN RGPSHSLAAR ILDETGVYKN LLQEIAQRVG APLPRYTTFR SGLGHQPVFT 130 140 150 160 170 180 GTVELAGITF TGDPAKNKKQ AEKNAAMAAW SSLKQLAKET SSSMPEPENI DELEQVIIAR 190 200 210 220 230 240 ALINYRIKEN IGTGSSSSAP VPFAKKFFMQ NLRPTSPQPS PATTSRILPF ICPKQPSRSS 250 260 270 280 290 300 RSSLAATSGI DRIMAAALES RSYQRPQQRF ANPPYVPMRQ FRSQCHGMAP PVTIRTAVPV 310 320 330 340 350 360 FSAPPMPPPP CTNNTQLPSS VYVPSLMRTA PPVRIAPPVT IRTAVPVFAS APPVRIRTAV 370 380 390 400 410 420 KPTVEAGETR ISSVQEKESI PVLPDSLEIG VEGSTITITD CEKTASKETE RAEFKDSSKG 430 EPETARERLE NLKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana." Hiraguri A., Itoh R., Kondo N., Nomura Y., Aizawa D., Murai Y., Koiwa H., Seki M., Shinozaki K., Fukuhara T. Plant Mol. Biol. 57:173-188(2005) [PubMed: 15821876] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, INTERACTION WITH DCL1; DCL5; DRB1 AND DRB5.
[5]	"The roles of plant dsRNA-binding proteins in RNAi-like pathways." Curtin S.J., Watson J.M., Smith N.A., Eamens A.L., Blanchard C.L., Waterhouse P.M. FEBS Lett. 582:2753-2760(2008) [PubMed: 18625233] [Abstract] Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006283 Genomic DNA. Translation: AAD20688.1. CP002685 Genomic DNA. Translation: AEC08114.1. AY034976 mRNA. Translation: AAK59481.1. AY062965 mRNA. Translation: AAL33811.1.
IPI	IPI00547657.
PIR	B84684.
RefSeq	NP_565672.1. NM_128398.3.
UniGene	At.13732.
3D structure databases
ProteinModelPortal	Q9SKN2.
SMR	Q9SKN2. Positions 1-70, 86-154.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SKN2. 8 interactions.
STRING	Q9SKN2.
Proteomic databases
PRIDE	Q9SKN2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G28380.1; AT2G28380.1; AT2G28380.
GeneID	817386.
GenomeReviews	Gene locus AT2G28380 in contig CT485783_GR.
KEGG	ath:AT2G28380.
NMPDR	fig|3702.1.peg.9889.
Organism-specific databases
TAIR	At2g28380.
Phylogenomic databases
GeneTree	EPGT00050000001224.
HOGENOM	HBG602072.
InParanoid	Q9SKN2.
OMA	FTGDPAK.
PhylomeDB	Q9SKN2.
ProtClustDB	CLSN2688512.
Gene expression databases
ArrayExpress	Q9SKN2.
Genevestigator	Q9SKN2.
Family and domain databases
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like. IPR011907. RNase_III_bac. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 2 hits.
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 2 hits. [Graphical view]
SMART	SM00358. DSRM. 2 hits. [Graphical view]
PROSITE	PS50137. DS_RBD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DRB2_ARATH

Accession

Primary (citable) accession number: Q9SKN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program