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Protein

EIN3-binding F-box protein 1

Gene

EBF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of SCF(EBF1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including EIN3 and EIL1). Regulator of the ethylene signaling cascade by modulating the stability of EIN3 and EIL1 proteins. Confers insensitivity to ethylene.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • negative regulation of ethylene-activated signaling pathway Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • response to ethylene Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Ethylene signaling pathway, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
EIN3-binding F-box protein 1
Alternative name(s):
F-box/LRR-repeat protein 6
Gene namesi
Name:EBF1
Synonyms:FBL6
Ordered Locus Names:At2g25490
ORF Names:F13B15.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G25490.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002722451 – 628EIN3-binding F-box protein 1Add BLAST628

Proteomic databases

PaxDbiQ9SKK0.
PRIDEiQ9SKK0.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Inductioni

EIN3-dependent induction by ethylene.1 Publication

Gene expression databases

GenevisibleiQ9SKK0. AT.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1, SKP1A/ASK1, SKP1B/ASK2, ASK11, ASK12, ASK13, ASK18, EIN3, and EIL1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ASK11O494843EBI-401198,EBI-401185
EIN3O246063EBI-401198,EBI-593576
SKP1AQ392554EBI-401198,EBI-532357
SKP1BQ9FHW75EBI-401198,EBI-604076
SRK2AP432912EBI-401198,EBI-401164

Protein-protein interaction databases

BioGridi2439. 14 interactors.
DIPiDIP-31329N.
IntActiQ9SKK0. 13 interactors.
STRINGi3702.AT2G25490.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SKK0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 109F-boxAdd BLAST49

Domaini

The F-box is necessary for the interaction with ASK proteins.By similarity

Sequence similaritiesi

Contains 1 F-box domain.Curated

Phylogenomic databases

eggNOGiENOG410IT20. Eukaryota.
ENOG410XPA4. LUCA.
HOGENOMiHOG000238885.
InParanoidiQ9SKK0.
KOiK14515.
OMAiAHAKQIN.
OrthoDBiEOG093604ZD.
PhylomeDBiQ9SKK0.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF13516. LRR_6. 3 hits.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 13 hits.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SKK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQIFSFAGE NDFYRRGAIY PNPKDASLLL SLGSFADVYF PPSKRSRVVA
60 70 80 90 100
PTIFSAFEKK PVSIDVLPDE CLFEIFRRLS GPQERSACAF VSKQWLTLVS
110 120 130 140 150
SIRQKEIDVP SKITEDGDDC EGCLSRSLDG KKATDVRLAA IAVGTAGRGG
160 170 180 190 200
LGKLSIRGSN SAKVSDLGLR SIGRSCPSLG SLSLWNVSTI TDNGLLEIAE
210 220 230 240 250
GCAQLEKLEL NRCSTITDKG LVAIAKSCPN LTELTLEACS RIGDEGLLAI
260 270 280 290 300
ARSCSKLKSV SIKNCPLVRD QGIASLLSNT TCSLAKLKLQ MLNVTDVSLA
310 320 330 340 350
VVGHYGLSIT DLVLAGLSHV SEKGFWVMGN GVGLQKLNSL TITACQGVTD
360 370 380 390 400
MGLESVGKGC PNMKKAIISK SPLLSDNGLV SFAKASLSLE SLQLEECHRV
410 420 430 440 450
TQFGFFGSLL NCGEKLKAFS LVNCLSIRDL TTGLPASSHC SALRSLSIRN
460 470 480 490 500
CPGFGDANLA AIGKLCPQLE DIDLCGLKGI TESGFLHLIQ SSLVKINFSG
510 520 530 540 550
CSNLTDRVIS AITARNGWTL EVLNIDGCSN ITDASLVSIA ANCQILSDLD
560 570 580 590 600
ISKCAISDSG IQALASSDKL KLQILSVAGC SMVTDKSLPA IVGLGSTLLG
610 620
LNLQQCRSIS NSTVDFLVER LYKCDILS
Length:628
Mass (Da):66,589
Last modified:May 1, 2000 - v1
Checksum:i805CD1A3D441F392
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ609238 Genomic RNA. Translation: CAE75864.1.
AC006300 Genomic DNA. Translation: AAD20708.1.
CP002685 Genomic DNA. Translation: AEC07708.1.
AY072205 mRNA. Translation: AAL60026.1.
AY091333 mRNA. Translation: AAM14272.1.
AK317560 mRNA. Translation: BAH20224.1.
AK229995 mRNA. Translation: BAF01819.1.
PIRiA84649.
RefSeqiNP_565597.1. NM_128106.4.
UniGeneiAt.24288.
At.67740.

Genome annotation databases

EnsemblPlantsiAT2G25490.1; AT2G25490.1; AT2G25490.
GeneIDi817087.
GrameneiAT2G25490.1; AT2G25490.1; AT2G25490.
KEGGiath:AT2G25490.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ609238 Genomic RNA. Translation: CAE75864.1.
AC006300 Genomic DNA. Translation: AAD20708.1.
CP002685 Genomic DNA. Translation: AEC07708.1.
AY072205 mRNA. Translation: AAL60026.1.
AY091333 mRNA. Translation: AAM14272.1.
AK317560 mRNA. Translation: BAH20224.1.
AK229995 mRNA. Translation: BAF01819.1.
PIRiA84649.
RefSeqiNP_565597.1. NM_128106.4.
UniGeneiAt.24288.
At.67740.

3D structure databases

ProteinModelPortaliQ9SKK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2439. 14 interactors.
DIPiDIP-31329N.
IntActiQ9SKK0. 13 interactors.
STRINGi3702.AT2G25490.1.

Proteomic databases

PaxDbiQ9SKK0.
PRIDEiQ9SKK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G25490.1; AT2G25490.1; AT2G25490.
GeneIDi817087.
GrameneiAT2G25490.1; AT2G25490.1; AT2G25490.
KEGGiath:AT2G25490.

Organism-specific databases

TAIRiAT2G25490.

Phylogenomic databases

eggNOGiENOG410IT20. Eukaryota.
ENOG410XPA4. LUCA.
HOGENOMiHOG000238885.
InParanoidiQ9SKK0.
KOiK14515.
OMAiAHAKQIN.
OrthoDBiEOG093604ZD.
PhylomeDBiQ9SKK0.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9SKK0.

Gene expression databases

GenevisibleiQ9SKK0. AT.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF13516. LRR_6. 3 hits.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 13 hits.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEBF1_ARATH
AccessioniPrimary (citable) accession number: Q9SKK0
Secondary accession number(s): B9DHK7, Q0WM37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.