Reviewed, UniProtKB/Swiss-Prot Q9SKK0 (EBF1_ARATH)

Names and origin

Protein names

Recommended name:
    EIN3-binding F-box protein 1
Alternative name(s):
    F-box/LRR-repeat protein 6

Gene names

Name:	EBF1
Synonyms:	FBL6
Ordered Locus Names:	At2g25490
ORF Names:	F13B15.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of SCF(EBF1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including EIN3 and EIL1). Regulator of the ethylene signaling cascade by modulating the stability of EIN3 and EIL1 proteins. Confers insensitivity to ethylene. Ref.1
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1, SKP1A/ASK1, SKP1B/ASK2, ASK11, ASK12, ASK13, ASK18, EIN3, and EIL1. Ref.1
Subcellular location	Nucleus. Ref.1
Tissue specificity	Ubiquitous. Ref.1
Induction	EIN3-dependent induction by ethylene. Ref.1
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ethylene signaling pathway Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ethylene mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of ethylene mediated signaling pathway Ref.1 Traceable author statement. Source: TAIR ubiquitin-dependent protein catabolic process Traceable author statement. Source: TAIR
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: TAIR
Molecular function	protein binding Ref.1 Ref.7 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AT4g34210	O49484	2	EBI-401198,EBI-401185
CUL1	Q94AH6	1	EBI-401198,EBI-532411
EIN3	O24606	3	EBI-401198,EBI-593576
SKP1A	Q39255	3	EBI-401198,EBI-532357
SKP1B	Q9FHW7	3	EBI-401198,EBI-604076
SRK2A	P43291	1	EBI-401198,EBI-401164

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	EIN3-binding F-box protein 1		PRO_0000272245
Regions
	Domain	61 – 109	49	F-box

Sequences

	Sequence		Length	Mass (Da)
	Q9SKK0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 805CD1A3D441F392	FASTA	628	66,589
	10 20 30 40 50 60 MSQIFSFAGE NDFYRRGAIY PNPKDASLLL SLGSFADVYF PPSKRSRVVA PTIFSAFEKK 70 80 90 100 110 120 PVSIDVLPDE CLFEIFRRLS GPQERSACAF VSKQWLTLVS SIRQKEIDVP SKITEDGDDC 130 140 150 160 170 180 EGCLSRSLDG KKATDVRLAA IAVGTAGRGG LGKLSIRGSN SAKVSDLGLR SIGRSCPSLG 190 200 210 220 230 240 SLSLWNVSTI TDNGLLEIAE GCAQLEKLEL NRCSTITDKG LVAIAKSCPN LTELTLEACS 250 260 270 280 290 300 RIGDEGLLAI ARSCSKLKSV SIKNCPLVRD QGIASLLSNT TCSLAKLKLQ MLNVTDVSLA 310 320 330 340 350 360 VVGHYGLSIT DLVLAGLSHV SEKGFWVMGN GVGLQKLNSL TITACQGVTD MGLESVGKGC 370 380 390 400 410 420 PNMKKAIISK SPLLSDNGLV SFAKASLSLE SLQLEECHRV TQFGFFGSLL NCGEKLKAFS 430 440 450 460 470 480 LVNCLSIRDL TTGLPASSHC SALRSLSIRN CPGFGDANLA AIGKLCPQLE DIDLCGLKGI 490 500 510 520 530 540 TESGFLHLIQ SSLVKINFSG CSNLTDRVIS AITARNGWTL EVLNIDGCSN ITDASLVSIA 550 560 570 580 590 600 ANCQILSDLD ISKCAISDSG IQALASSDKL KLQILSVAGC SMVTDKSLPA IVGLGSTLLG 610 620 LNLQQCRSIS NSTVDFLVER LYKCDILS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"EIN3-dependent regulation of plant ethylene hormone signaling by two Arabidopsis F box proteins: EBF1 and EBF2." Potuschak T., Lechner E., Parmentier Y., Yanagisawa S., Grava S., Koncz C., Genschik P. Cell 115:679-689(2003) [PubMed: 14675533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH CUL1; SKP1A/ASK1; SKP1B/ASK2; EIN3 AND EIL1.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-628. Strain: cv. Columbia. Tissue: Rosette leaf.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 245-384. Strain: cv. Columbia.
[6]	"F-box proteins in Arabidopsis." Xiao W., Jang J.-C. Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis." Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D. Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002) [PubMed: 12169662] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK11; ASK13 AND ASK18.
[8]	"Expression and interaction analysis of Arabidopsis Skp1-related genes." Takahashi N., Kuroda H., Kuromori T., Hirayama T., Seki M., Shinozaki K., Shimada H., Matsui M. Plant Cell Physiol. 45:83-91(2004) [PubMed: 14749489] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK11 AND ASK12.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ609238 Genomic RNA. Translation: CAE75864.1. AC006300 Genomic DNA. Translation: AAD20708.1. AY072205 mRNA. Translation: AAL60026.1. AY091333 mRNA. Translation: AAM14272.1. AK317560 mRNA. Translation: BAH20224.1. AK229995 mRNA. Translation: BAF01819.1.
IPI	IPI00530647.
PIR	A84649.
RefSeq	NP_565597.1.
UniGene	At.24288 At.67740
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SKK0. 10 interactions.
Genome annotation databases
GeneID	817087.
GenomeReviews	Gene locus AT2G25490 in contig CT485783_GR.
KEGG	ath:AT2G25490.
NMPDR	fig|3702.1.peg.9563.
Organism-specific databases
TAIR	At2g25490.
Phylogenomic databases
OMA	Q9SKK0. EIANGCH.
Gene expression databases
ArrayExpress	Q9SKK0.
Family and domain databases
InterPro	IPR001810. F-box. IPR006553. Leu-rich_rpt_Cys-con_subtyp. [Graphical view]
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. SM00367. LRR_CC. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EBF1_ARATH

Accession

Primary (citable) accession number: Q9SKK0
Secondary accession number(s): B9DHK7, Q0WM37

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)