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Protein

EIN3-binding F-box protein 1

Gene

EBF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of SCF(EBF1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including EIN3 and EIL1). Regulator of the ethylene signaling cascade by modulating the stability of EIN3 and EIL1 proteins. Confers insensitivity to ethylene.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • negative regulation of ethylene-activated signaling pathway Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • response to ethylene Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Biological processEthylene signaling pathway, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
EIN3-binding F-box protein 1
Alternative name(s):
F-box/LRR-repeat protein 6
Gene namesi
Name:EBF1
Synonyms:FBL6
Ordered Locus Names:At2g25490
ORF Names:F13B15.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G25490
TAIRilocus:2040105 AT2G25490

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002722451 – 628EIN3-binding F-box protein 1Add BLAST628

Proteomic databases

PaxDbiQ9SKK0

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Inductioni

EIN3-dependent induction by ethylene.1 Publication

Gene expression databases

ExpressionAtlasiQ9SKK0 baseline and differential
GenevisibleiQ9SKK0 AT

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CUL1, SKP1A/ASK1, SKP1B/ASK2, ASK11, ASK12, ASK13, ASK18, EIN3, and EIL1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi2439, 14 interactors
DIPiDIP-31329N
IntActiQ9SKK0, 13 interactors
STRINGi3702.AT2G25490.1

Structurei

3D structure databases

ProteinModelPortaliQ9SKK0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 109F-boxAdd BLAST49

Domaini

The F-box is necessary for the interaction with ASK proteins.By similarity

Phylogenomic databases

eggNOGiENOG410IT20 Eukaryota
ENOG410XPA4 LUCA
HOGENOMiHOG000238885
InParanoidiQ9SKK0
KOiK14515
OMAiCEGCLSR
OrthoDBiEOG093604ZD
PhylomeDBiQ9SKK0

Family and domain databases

Gene3Di3.80.10.10, 4 hits
InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR001611 Leu-rich_rpt
IPR006553 Leu-rich_rpt_Cys-con_subtyp
IPR032675 LRR_dom_sf
PfamiView protein in Pfam
PF12937 F-box-like, 1 hit
PF13516 LRR_6, 3 hits
SMARTiView protein in SMART
SM00256 FBOX, 1 hit
SM00367 LRR_CC, 13 hits
SUPFAMiSSF81383 SSF81383, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SKK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQIFSFAGE NDFYRRGAIY PNPKDASLLL SLGSFADVYF PPSKRSRVVA
60 70 80 90 100
PTIFSAFEKK PVSIDVLPDE CLFEIFRRLS GPQERSACAF VSKQWLTLVS
110 120 130 140 150
SIRQKEIDVP SKITEDGDDC EGCLSRSLDG KKATDVRLAA IAVGTAGRGG
160 170 180 190 200
LGKLSIRGSN SAKVSDLGLR SIGRSCPSLG SLSLWNVSTI TDNGLLEIAE
210 220 230 240 250
GCAQLEKLEL NRCSTITDKG LVAIAKSCPN LTELTLEACS RIGDEGLLAI
260 270 280 290 300
ARSCSKLKSV SIKNCPLVRD QGIASLLSNT TCSLAKLKLQ MLNVTDVSLA
310 320 330 340 350
VVGHYGLSIT DLVLAGLSHV SEKGFWVMGN GVGLQKLNSL TITACQGVTD
360 370 380 390 400
MGLESVGKGC PNMKKAIISK SPLLSDNGLV SFAKASLSLE SLQLEECHRV
410 420 430 440 450
TQFGFFGSLL NCGEKLKAFS LVNCLSIRDL TTGLPASSHC SALRSLSIRN
460 470 480 490 500
CPGFGDANLA AIGKLCPQLE DIDLCGLKGI TESGFLHLIQ SSLVKINFSG
510 520 530 540 550
CSNLTDRVIS AITARNGWTL EVLNIDGCSN ITDASLVSIA ANCQILSDLD
560 570 580 590 600
ISKCAISDSG IQALASSDKL KLQILSVAGC SMVTDKSLPA IVGLGSTLLG
610 620
LNLQQCRSIS NSTVDFLVER LYKCDILS
Length:628
Mass (Da):66,589
Last modified:May 1, 2000 - v1
Checksum:i805CD1A3D441F392
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ609238 Genomic RNA Translation: CAE75864.1
AC006300 Genomic DNA Translation: AAD20708.1
CP002685 Genomic DNA Translation: AEC07708.1
AY072205 mRNA Translation: AAL60026.1
AY091333 mRNA Translation: AAM14272.1
AK317560 mRNA Translation: BAH20224.1
AK229995 mRNA Translation: BAF01819.1
PIRiA84649
RefSeqiNP_565597.1, NM_128106.4
UniGeneiAt.24288
At.67740

Genome annotation databases

EnsemblPlantsiAT2G25490.1; AT2G25490.1; AT2G25490
GeneIDi817087
GrameneiAT2G25490.1; AT2G25490.1; AT2G25490
KEGGiath:AT2G25490

Similar proteinsi

Entry informationi

Entry nameiEBF1_ARATH
AccessioniPrimary (citable) accession number: Q9SKK0
Secondary accession number(s): B9DHK7, Q0WM37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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