Reviewed, UniProtKB/Swiss-Prot Q9SKJ5 (CESAA_ARATH)

Names and origin

Protein names

Recommended name:
    Probable cellulose synthase A catalytic subunit 10 [UDP-forming]
      Short name=AtCesA10
      Short name=AtCesA13
    EC=2.4.1.12

Gene names

Name:	CESA10
Synonyms:	CESA13
Ordered Locus Names:	At2g25540
ORF Names:	F13B15.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1065 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation By similarity.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Glycan metabolism; plant cellulose biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed in young plants, stems, flowers and inflorescences. Ref.3 Ref.4
Developmental stage	Not found in embryos. Ref.3
Sequence similarities	Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell membrane Membrane
Domain	Coiled coil Transmembrane Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1065	1065	Probable cellulose synthase A catalytic subunit 10 [UDP-forming]		PRO_0000166376
Regions
	Topological domain	1 – 257	257	Cytoplasmic Potential
	Transmembrane	258 – 278	21	Potential
	Topological domain	279 – 286	8	Extracellular Potential
	Transmembrane	287 – 307	21	Potential
	Topological domain	308 – 850	543	Cytoplasmic Potential
	Transmembrane	851 – 871	21	Potential
	Topological domain	872 – 876	5	Extracellular Potential
	Transmembrane	877 – 897	21	Potential
	Topological domain	898 – 912	15	Cytoplasmic Potential
	Transmembrane	913 – 933	21	Potential
	Topological domain	934 – 963	30	Extracellular Potential
	Transmembrane	964 – 984	21	Potential
	Topological domain	985 – 995	11	Cytoplasmic Potential
	Transmembrane	996 – 1016	21	Potential
	Topological domain	1017 – 1025	9	Extracellular Potential
	Transmembrane	1026 – 1046	21	Potential
	Topological domain	1047 – 1065	19	Cytoplasmic Potential
	Zinc finger	33 – 79	47	RING-type; degenerate
	Coiled coil	436 – 463	28	Potential
Sites
	Active site	382	1	Potential
	Active site	767	1	Potential
	Metal binding	33	1	Zinc 1 By similarity
	Metal binding	36	1	Zinc 1 By similarity
	Metal binding	52	1	Zinc 2 By similarity
	Metal binding	55	1	Zinc 2 By similarity
	Metal binding	60	1	Zinc 1 By similarity
	Metal binding	63	1	Zinc 1 By similarity
	Metal binding	75	1	Zinc 2 By similarity
	Metal binding	78	1	Zinc 2 By similarity
	Binding site	548	1	Substrate Potential
	Binding site	550	1	Substrate Potential
Amino acid modifications
	Glycosylation	940	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9SKJ5-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F2AE36C9492A6076	FASTA	1,065	120,617
	10 20 30 40 50 60 MVAGSYRRYE FVRNRDDSDD GLKPLKDLNG QICQICGDDV GLTKTGNVFV ACNECGFPLC 70 80 90 100 110 120 QSCYEYERKD GSQCCPQCKA RFRRHNGSPR VEVDEKEDDV NDIENEFDYT QGNNKARLPH 130 140 150 160 170 180 RAEEFSSSSR HEESLPVSLL THGHPVSGEI PTPDRNATLS PCIDPQLPGI YQLLLLPVRI 190 200 210 220 230 240 LDPSKDLNSY GLVNVDWKKR IQGWKLKQDK NMIHMTGKYH EGKGGEFEGT GSNGDELQMV 250 260 270 280 290 300 DDARLPMSRV VHFPSARMTP YRIVIVLRLI ILGVFLHYRT THPVKDAYAL WLTSVICEIW 310 320 330 340 350 360 FAFSWLLDQF PKWYPINRET FLDRLALRYD RDGEPSQLAP VDVFVSTVDP MKEPPLVTAN 370 380 390 400 410 420 TVLSILAVDY PVDKVACYVS DDGSAMLTFE ALSETAEFSK KWVPFCKKFN IEPRAPEFYF 430 440 450 460 470 480 SQKIDYLKDK IQPSFVKERR AMKREYEEFK VRINILVAKA QKIPEDGWTM EDGTSWPGNN 490 500 510 520 530 540 PRDHPGMIQV FLGHSGGLDT DGNELPRLIY VSREKRPGFQ HHKKAGAMNA LIRVSAVLTN 550 560 570 580 590 600 GAYLLNVDCD HYFNNSKAIK EAMCFMMDPA IGKKCCYVQF PQRFDGIDLH DRYANRNTVF 610 620 630 640 650 660 FDINLKGLDG IQGPVYVGTG CCFNRQALYG YDPVLTEEDL EPNIIVKSCF GSRKKGKSRK 670 680 690 700 710 720 IPNYEDNRSI KRSDSNVPLF NMEDIDEDVE GYEDEMSLLV SQKRLEKRFG QSPVFIAATF 730 740 750 760 770 780 MEQGGLPSTT NPLTLLKEAI HVISCGYEAK TDWGKEIGWI YGSVTEDILT GFKMHARGWI 790 800 810 820 830 840 SIYCVPSRPA FKGSAPINLS DRLNQVLRWA LGSIEILLSR HCPIWYGYNG RLKLLERIAY 850 860 870 880 890 900 INTIVYPITS IPLLAYCMLP AFCLITNTFI IPEISNLASL CFMLLFASIY ASAILELKWS 910 920 930 940 950 960 DVALEDWWRN EQFWVIGGTS AHLFAVFQGL LKVFAGIDTN FTVTSKASDE DGDFAELYVF 970 980 990 1000 1010 1020 KWTSLLIPPT TILLVNLVGI VAGVSYAINS GYQSWGPLMG KLLFAFWVVA HLYPFLKGLL 1030 1040 1050 1060 GRQNRTPTIV IVWSALLASI FSLLWVRINP FVSTTGVMSN SFMGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Higher plant cellulose synthases." Richmond T. Genome Biol. 1:REVIEWS3001.1-REVIEWS3001.6(2000) [PubMed: 11178255] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[3]	"Functional analysis of the cellulose synthase genes CesA1, CesA2, and CesA3 in Arabidopsis." Burn J.E., Hocart C.H., Birch R.J., Cork A.C., Williamson R.E. Plant Physiol. 129:797-807(2002) [PubMed: 12068120] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]	"Genetic complexity of cellulose synthase A gene function in Arabidopsis embryogenesis." Beeckman T., Przemeck G.K.H., Stamatiou G., Lau R., Terryn N., De Rycke R., Inze D., Berleth T. Plant Physiol. 130:1883-1893(2002) [PubMed: 12481071] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC006300 Genomic DNA. Translation: AAD20713.1.
IPI	IPI00530505.
PIR	F84649.
RefSeq	NP_180124.1.
UniGene	At.27491
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Proteomic databases
PRIDE	Q9SKJ5.
Genome annotation databases
GeneID	817092.
GenomeReviews	Gene locus AT2G25540 in contig CT485783_GR.
KEGG	ath:AT2G25540.
NMPDR	fig|3702.1.peg.9568.
Organism-specific databases
GeneFarm	5093. 484.
TAIR	At2g25540.
Enzyme and pathway databases
BRENDA	2.4.1.12. 302.
Gene expression databases
ArrayExpress	Q9SKJ5.
GermOnline	AT2G25540. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005150. Cellulose_synth. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF03552. Cellulose_synt. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CESAA_ARATH

Accession

Primary (citable) accession number: Q9SKJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)