Reviewed, UniProtKB/Swiss-Prot Q9SKB7 (IP5PE_ARATH)

Names and origin

Protein names

Recommended name:
    Type II inositol-1,4,5-trisphosphate 5-phosphatase 14
      Short name=At5PTase14
    EC=3.1.3.36

Gene names

Name:	5PTASE14
Ordered Locus Names:	At2g31830
ORF Names:	F20M17.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1144 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has phosphatase activity toward PtdIns(4,5)P2, PtdIns(3,4,5)P3 and Ins(1,4,5)P3. Ref.1
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Cofactor	Magnesium. Ref.1
Tissue specificity	Expressed in young seedlings and flowers. Ref.1
Induction	Slightly reduced by dark treatment. Ref.1
Sequence similarities	Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family. Contains 5 WD repeats.
Biophysicochemical properties	Kinetic parameters: KM=101 µM for PtdIns(4,5) KM=211 µM for PtdIns(3,4,5)P3 KM=395 µM for Ins(1,4,5)P3

Ontologies

Keywords
Domain	Repeat WD repeat
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1144	1144	Type II inositol-1,4,5-trisphosphate 5-phosphatase 14		PRO_0000359744
Regions
	Repeat	158 – 196	39	WD 1
	Repeat	216 – 255	40	WD 2
	Repeat	269 – 307	39	WD 3
	Repeat	445 – 483	39	WD 4
	Repeat	524 – 561	38	WD 5
	Compositional bias	758 – 763	6	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	Q9SKB7-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 414BBDDD0DAB7249	FASTA	1,144	125,708
	10 20 30 40 50 60 MDSVIIEPDE REALASLVPA HPLPPRKTHS YVEQCEQKPH HPIRKYSLDE GSRSVTSDSE 70 80 90 100 110 120 AVYFDSSDGE FSTEGVAIVD GRTSGERGNG EECGFVTPPS KPASQGGGND GGREDDIESL 130 140 150 160 170 180 PEFIGAGGGL DVFKVPVRAA VNPGRPPCLE LRPHPLRETQ TGKFLRNIAC TESQLWAGQE 190 200 210 220 230 240 NGVRFWNLEE AYEVGCGLGG QVRRGDEDTA PFHESVPTSP ALCLLVDHGN RLVWTGHKDG 250 260 270 280 290 300 KIRAWKMNQP NTTTADDSKP FKERLSWQAH RGPVNYIVIS SYGDMWSCSD GGVIKIWTLD 310 320 330 340 350 360 SLEKSLVLKL EEKHMAALLV ERSGIDLRSQ VTVNGTCSIS SSDVKFLLVD TVKAKVWAVQ 370 380 390 400 410 420 HLSFSLWDAQ NKELLKVFNI DGQVENRVDM PPTQGQQVED TKAKFFSAPK KEKSQGFLQR 430 440 450 460 470 480 SRHAIMGAAG AVRRAATRSA GAFAEDTRKV EAIAIAADGS IWTGSMNGVI AQWDGNGSRL 490 500 510 520 530 540 REVNHHQQAV LCFCTFGDRI YVGYSSGYIQ VLDLGGKLIA SWVSHNEPVI KLAAGGGFIF 550 560 570 580 590 600 SLATHGGVRG WYVTSPGPLD SLIRTELSQK EMAYARQDSV KILIGTWNVG EGRASRGALV 610 620 630 640 650 660 SWLGSAVSDV GIVAIGLQEV DMGAGFLAMS TAKETVGVEG SAVGQWWLDA IGNALDERNT 670 680 690 700 710 720 FERMGSRQLA GLLISLWVRK SIRTHVGDLD VAAVPCGFGR AIGNKGGVGL RIRVYDRIMC 730 740 750 760 770 780 FVNCHLAAHL EAVTRRNADF NHIYRSMVFS KGQSVYTAAA AGASTSAQAL KNNPNTNNST 790 800 810 820 830 840 EEEKSHLASA DLVAFFGDFN YRLFGITYDE ARDFISHRSF DWLREKDQLR QEMNEGKVFQ 850 860 870 880 890 900 GMREALITFP PTYKFEKNKP GLGGYDSGEK KRIPAWCDRV IYRDNQSISY TECSLKCPVV 910 920 930 940 950 960 SSTIMYEACM DVTESDHKPV RCKLHANIAH TDKSVRRQEL GKIVKSNEKL RAMFEELKSV 970 980 990 1000 1010 1020 PETSVSTNNI LLHSQDTFIF TIRNTSNSSR AIFNIVCKGQ TLVREDGEEP DNHSRGTFGL 1030 1040 1050 1060 1070 1080 PRWLEVSPGA GIIKPDASLQ VKVHHEDSHN SEEFIDGIQQ NSLSEESSDK EVTLIIIVQG 1090 1100 1110 1120 1130 1140 SCSTRTISHS IKVRHCSSAA KSLSLVHSKT TTMTKNLEGS TRYQTDANRG GSTRHRTDDS TRRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and biochemical characterization of three WD-repeat-domain-containing inositol polyphosphate 5-phosphatases in Arabidopsis thaliana." Zhong R., Ye Z.-H. Plant Cell Physiol. 45:1720-1728(2004) [PubMed: 15574849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY761189 mRNA. Translation: AAV87316.1. AY761193 Genomic DNA. Translation: AAV87320.1. AC006533 Genomic DNA. Translation: AAD32289.1.
IPI	IPI00525979.
PIR	F84725.
RefSeq	NP_180742.1.
UniGene	At.51207
3D structure databases
HSSP	HSSP built from PDB template 1I9Z based on UniProtKB O43001.
SMR	Q9SKB7. Positions 175-302, 451-551, 557-949.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SKB7.
Proteomic databases
PRIDE	Q9SKB7.
Genome annotation databases
GeneID	817740.
GenomeReviews	Gene locus AT2G31830 in contig CT485783_GR.
KEGG	ath:AT2G31830.
NMPDR	fig|3702.1.peg.10244.
Organism-specific databases
GeneFarm	3136. 267.
TAIR	At2g31830.
Phylogenomic databases
eggNOG	KOG0565.
HOGENOM	HBG319261.
InParanoid	Q9SKB7.
PhylomeDB	Q9SKB7.
Enzyme and pathway databases
BRENDA	3.1.3.36. 302.
Gene expression databases
ArrayExpress	Q9SKB7.
Genevestigator	Q9SKB7.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. IPR011044. Quino_amine_DH_bsu. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SMART	SM00128. IPPc. 1 hit. SM00320. WD40. 4 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IP5PE_ARATH

Accession

Primary (citable) accession number: Q9SKB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)