Reviewed,
UniProtKB/Swiss-Prot Q9SKB7 (IP5PE_ARATH)
Last modified
February 9, 2010.
Version 67.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Type II inositol-1,4,5-trisphosphate 5-phosphatase 14 Short name=At5PTase14 EC=3.1.3.36 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 1144 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Has phosphatase activity toward PtdIns(4,5)P2, PtdIns(3,4,5)P3 and Ins(1,4,5)P3. Ref.1 |
| Catalytic activity | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate. |
| Cofactor | Magnesium. Ref.1 |
| Tissue specificity | Expressed in young seedlings and flowers. Ref.1 |
| Induction | Slightly reduced by dark treatment. Ref.1 |
| Sequence similarities | Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family. Contains 5 WD repeats. |
| Biophysicochemical properties | Kinetic parameters: KM=101 µM for PtdIns(4,5) KM=211 µM for PtdIns(3,4,5)P3 KM=395 µM for Ins(1,4,5)P3 |
Ontologies
| Keywords | |
|---|---|
| Domain | Repeat WD repeat |
| Ligand | Magnesium Metal-binding |
| Molecular function | Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Molecular function | magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol-4,5-bisphosphate 5-phosphatase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1144 | 1144 | Type II inositol-1,4,5-trisphosphate 5-phosphatase 14 | PRO_0000359744 | |||||
Regions | |||||||||
| Repeat | 158 – 196 | 39 | WD 1 | ||||||
| Repeat | 216 – 255 | 40 | WD 2 | ||||||
| Repeat | 269 – 307 | 39 | WD 3 | ||||||
| Repeat | 445 – 483 | 39 | WD 4 | ||||||
| Repeat | 524 – 561 | 38 | WD 5 | ||||||
| Compositional bias | 758 – 763 | 6 | Poly-Ala | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular and biochemical characterization of three WD-repeat-domain-containing inositol polyphosphate 5-phosphatases in Arabidopsis thaliana." Zhong R., Ye Z.-H. Plant Cell Physiol. 45:1720-1728(2004) [PubMed: 15574849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C.Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY761189 mRNA. Translation: AAV87316.1. AY761193 Genomic DNA. Translation: AAV87320.1. AC006533 Genomic DNA. Translation: AAD32289.1. |
| IPI | IPI00525979. |
| PIR | F84725. |
| RefSeq | NP_180742.1. |
| UniGene | At.51207 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1I9Z based on UniProtKB O43001. |
| SMR | Q9SKB7. Positions 175-302, 451-551, 557-949. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9SKB7. |
Proteomic databases | |
| PRIDE | Q9SKB7. |
Genome annotation databases | |
| GeneID | 817740. |
| GenomeReviews | Gene locus AT2G31830 in contig CT485783_GR. |
| KEGG | ath:AT2G31830. |
| NMPDR | fig|3702.1.peg.10244. |
Organism-specific databases | |
| GeneFarm | 3136. 267. |
| TAIR | At2g31830. |
Phylogenomic databases | |
| eggNOG | KOG0565. |
| HOGENOM | HBG319261. |
| InParanoid | Q9SKB7. |
| PhylomeDB | Q9SKB7. |
Enzyme and pathway databases | |
| BRENDA | 3.1.3.36. 302. |
Gene expression databases | |
| ArrayExpress | Q9SKB7. |
| Genevestigator | Q9SKB7. |
Family and domain databases | |
| InterPro | IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. IPR011044. Quino_amine_DH_bsu. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. [Graphical view] |
| Gene3D | G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit. |
| Pfam | PF03372. Exo_endo_phos. 1 hit. [Graphical view] |
| SMART | SM00128. IPPc. 1 hit. SM00320. WD40. 4 hits. [Graphical view] |
| PROSITE | PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | IP5PE_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9SKB7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


