Type II inositol 1,4,5-trisphosphate 5-phosphatase 14 - Arabidopsis thaliana (Mouse-ear cress)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Type II inositol 1,4,5-trisphosphate 5-phosphatase 14
Short name=At5PTase14
EC=3.1.3.36

Gene names

Name:	5PTASE14
Ordered Locus Names:	At2g31830
ORF Names:	F20M17.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1144 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has phosphatase activity toward PtdIns(4,5)P2, PtdIns(3,4,5)P3 and Ins(1,4,5)P3. Ref.1
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H₂O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Cofactor	Magnesium. Ref.1
Tissue specificity	Expressed in young seedlings and flowers. Ref.1
Induction	Slightly reduced by dark treatment. Ref.1
Sequence similarities	Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. Contains 5 WD repeats.
Biophysicochemical properties	Kinetic parameters: K_M=101 µM for PtdIns(4,5) Ref.1 K_M=211 µM for PtdIns(3,4,5)P3 K_M=395 µM for Ins(1,4,5)P3

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidylinositol phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SKB7-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1144

1144

Type II inositol 1,4,5-trisphosphate 5-phosphatase 14

PRO_0000359744

Regions

Repeat

158 – 196

39

WD 1

Repeat

216 – 255

40

WD 2

Repeat

269 – 307

39

WD 3

Repeat

445 – 483

39

WD 4

Repeat

524 – 561

38

WD 5

Compositional bias

758 – 763

6

Poly-Ala

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 414BBDDD0DAB7249
Show »

FASTA

1,144

125,708

        10         20         30         40         50         60 
MDSVIIEPDE REALASLVPA HPLPPRKTHS YVEQCEQKPH HPIRKYSLDE GSRSVTSDSE 

        70         80         90        100        110        120 
AVYFDSSDGE FSTEGVAIVD GRTSGERGNG EECGFVTPPS KPASQGGGND GGREDDIESL 

       130        140        150        160        170        180 
PEFIGAGGGL DVFKVPVRAA VNPGRPPCLE LRPHPLRETQ TGKFLRNIAC TESQLWAGQE 

       190        200        210        220        230        240 
NGVRFWNLEE AYEVGCGLGG QVRRGDEDTA PFHESVPTSP ALCLLVDHGN RLVWTGHKDG 

       250        260        270        280        290        300 
KIRAWKMNQP NTTTADDSKP FKERLSWQAH RGPVNYIVIS SYGDMWSCSD GGVIKIWTLD 

       310        320        330        340        350        360 
SLEKSLVLKL EEKHMAALLV ERSGIDLRSQ VTVNGTCSIS SSDVKFLLVD TVKAKVWAVQ 

       370        380        390        400        410        420 
HLSFSLWDAQ NKELLKVFNI DGQVENRVDM PPTQGQQVED TKAKFFSAPK KEKSQGFLQR 

       430        440        450        460        470        480 
SRHAIMGAAG AVRRAATRSA GAFAEDTRKV EAIAIAADGS IWTGSMNGVI AQWDGNGSRL 

       490        500        510        520        530        540 
REVNHHQQAV LCFCTFGDRI YVGYSSGYIQ VLDLGGKLIA SWVSHNEPVI KLAAGGGFIF 

       550        560        570        580        590        600 
SLATHGGVRG WYVTSPGPLD SLIRTELSQK EMAYARQDSV KILIGTWNVG EGRASRGALV 

       610        620        630        640        650        660 
SWLGSAVSDV GIVAIGLQEV DMGAGFLAMS TAKETVGVEG SAVGQWWLDA IGNALDERNT 

       670        680        690        700        710        720 
FERMGSRQLA GLLISLWVRK SIRTHVGDLD VAAVPCGFGR AIGNKGGVGL RIRVYDRIMC 

       730        740        750        760        770        780 
FVNCHLAAHL EAVTRRNADF NHIYRSMVFS KGQSVYTAAA AGASTSAQAL KNNPNTNNST 

       790        800        810        820        830        840 
EEEKSHLASA DLVAFFGDFN YRLFGITYDE ARDFISHRSF DWLREKDQLR QEMNEGKVFQ 

       850        860        870        880        890        900 
GMREALITFP PTYKFEKNKP GLGGYDSGEK KRIPAWCDRV IYRDNQSISY TECSLKCPVV 

       910        920        930        940        950        960 
SSTIMYEACM DVTESDHKPV RCKLHANIAH TDKSVRRQEL GKIVKSNEKL RAMFEELKSV 

       970        980        990       1000       1010       1020 
PETSVSTNNI LLHSQDTFIF TIRNTSNSSR AIFNIVCKGQ TLVREDGEEP DNHSRGTFGL 

      1030       1040       1050       1060       1070       1080 
PRWLEVSPGA GIIKPDASLQ VKVHHEDSHN SEEFIDGIQQ NSLSEESSDK EVTLIIIVQG 

      1090       1100       1110       1120       1130       1140 
SCSTRTISHS IKVRHCSSAA KSLSLVHSKT TTMTKNLEGS TRYQTDANRG GSTRHRTDDS 


TRRG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and biochemical characterization of three WD-repeat-domain-containing inositol polyphosphate 5-phosphatases in Arabidopsis thaliana." Zhong R., Ye Z.-H. Plant Cell Physiol. 45:1720-1728(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY761189 mRNA. Translation: AAV87316.1. AY761193 Genomic DNA. Translation: AAV87320.1. AC006533 Genomic DNA. Translation: AAD32289.1. CP002685 Genomic DNA. Translation: AEC08590.1.
IPI	IPI00525979.
PIR	F84725.
RefSeq	NP_180742.1. NM_128741.2.
UniGene	At.51207.
3D structure databases
HSSP	HSSP built from PDB template 1I9Z based on UniProtKB O43001.
ProteinModelPortal	Q9SKB7.
SMR	Q9SKB7. Positions 270-310, 443-556, 578-920.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G31830.1-P.
Proteomic databases
PaxDb	Q9SKB7.
PRIDE	Q9SKB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G31830.1; AT2G31830.1; AT2G31830.
GeneID	817740.
KEGG	ath:AT2G31830.
Organism-specific databases
GeneFarm	3136. 267.
TAIR	At2g31830.
Phylogenomic databases
eggNOG	COG5411.
HOGENOM	HOG000242921.
InParanoid	Q9SKB7.
PhylomeDB	Q9SKB7.
ProtClustDB	CLSN2683268.
Enzyme and pathway databases
BRENDA	3.1.3.36. 399.
Gene expression databases
ArrayExpress	Q9SKB7.
Genevestigator	Q9SKB7.
Family and domain databases
Gene3D	2.130.10.10. 2 hits.
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SMART	SM00128. IPPc. 1 hit. SM00320. WD40. 4 hits. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IP5PE_ARATH

Accession

Primary (citable) accession number: Q9SKB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents