Reviewed, UniProtKB/Swiss-Prot Q9SKB3 (PARG1_ARATH)

Names and origin

Protein names

Recommended name:
    Poly(ADP-ribose) glycohydrolase 1
    EC=3.2.1.143

Gene names

Name:	PARG1
Synonyms:	TEJ
Ordered Locus Names:	At2g31870, At2g31875
ORF Names:	F20M17.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase By similarity. Involved in establishing period length of the circadian oscillator. May regulate post-translational poly(ADP-ribosyl)ation of an oscillator component.
Catalytic activity	Hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
Sequence similarities	Belongs to the poly(ADP-ribose) glycohydrolase family.

Ontologies

Keywords
Biological process	Biological rhythms
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	poly(ADP-ribose) glycohydrolase activity Ref.1 Inferred from mutant phenotype. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SKB3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	Poly(ADP-ribose) glycohydrolase 1		PRO_0000288791
Experimental info
	Mutagenesis	262	1	G → E in tej; loss of function. Ref.1
	Sequence conflict	170	1	V → I in BAD95273. Ref.3
	Sequence conflict	408	1	Missing in BAD95273. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: F1A79FDA157C3329	FASTA	548	62,170
	10 20 30 40 50 60 MENREDLNSI LPYLPLVIRS SSLYWPPRVV EALKAMSEGP SHSQVDSGEV LRQAIFDMRR 70 80 90 100 110 120 SLSFSTLEPS ASNGYAFLFD ELIDEKESKR WFDEIIPALA SLLLQFPSLL EVHFQNADNI 130 140 150 160 170 180 VSGIKTGLRL LNSQQAGIVF LSQELIGALL ACSFFCLFPD DNRGAKHLPV INFDHLFASL 190 200 210 220 230 240 YISYSQSQES KIRCIMHYFE RFCSCVPIGI VSFERKITAA PDADFWSKSD VSLCAFKVHS 250 260 270 280 290 300 FGLIEDQPDN ALEVDFANKY LGGGSLSRGC VQEEIRFMIN PELIAGMLFL PRMDDNEAIE 310 320 330 340 350 360 IVGAERFSCY TGYASSFRFA GEYIDKKAMD PFKRRRTRIV AIDALCTPKM RHFKDICLLR 370 380 390 400 410 420 EINKALCGFL NCSKAWEHQN IFMDEGDNEI QLVRNGRDSG LLRTETTASH RTPLNDVEMN 430 440 450 460 470 480 REKPANNLIR DFYVEGVDNE DHEDDGVATG NWGCGVFGGD PELKATIQWL AASQTRRPFI 490 500 510 520 530 540 SYYTFGVEAL RNLDQVTKWI LSHKWTVGDL WNMMLEYSAQ RLYKQTSVGF FSWLLPSLAT TNKAIQPP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"tej defines a role for poly(ADP-ribosyl)ation in establishing period length of the arabidopsis circadian oscillator." Panda S., Poirier G.G., Kay S.A. Dev. Cell 3:51-61(2002) [PubMed: 12110167] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF GLY-262, FUNCTION. Strain: cv. C24.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF394690 mRNA. Translation: AAK72256.1. AC006533 Genomic DNA. Translation: AAD32285.2. AK222165 mRNA. Translation: BAD95273.1.
IPI	IPI00548019.
PIR	B84726.
RefSeq	NP_565730.1.
UniGene	At.26160
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	817745.
GenomeReviews	Gene locus AT2G31870 in contig CT485783_GR.
NMPDR	fig|3702.1.peg.10250.
Organism-specific databases
TAIR	At2g31870.
Phylogenomic databases
OMA	CLLREIN.
Enzyme and pathway databases
BRENDA	3.2.1.143. 302.
Gene expression databases
Genevestigator	Q9SKB3.
Family and domain databases
InterPro	IPR007724. Poly_GlycHdrlase. [Graphical view]
PANTHER	PTHR12837. Poly_glchydro. 1 hit.
Pfam	PF05028. PARG_cat. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PARG1_ARATH

Accession

Primary (citable) accession number: Q9SKB3
Secondary accession number(s): Q56W82, Q94ET7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)