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Protein

Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1

Gene

SOBIR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Acting as a counterplayer of BIR1, promotes the activation of plant defense and cell death (PubMed:19616764). Component of the RLP23-SOBIR1-BAK1 complex that mediates NLP-triggered immunity (PubMed:27251392). Functions as an inhibitor/regulator of abscission, probably by regulating membrane trafficking during abscission (PubMed:20081191).3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei377ATPPROSITE-ProRule annotation1
Active sitei489Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi353 – 361ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB
  • protein tyrosine kinase activity Source: UniProtKB
  • transmembrane receptor protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • positive regulation of cell death Source: TAIR
  • positive regulation of defense response Source: TAIR

Keywordsi

Molecular functionKinase, Receptor, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
Biological processPlant defense
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1 (EC:2.7.10.1, EC:2.7.11.1)
Alternative name(s):
Protein EVERSHED
Protein SUPPRESSOR OF BIR1-1
Gene namesi
Name:SOBIR1
Synonyms:EVR
Ordered Locus Names:At2g31880
ORF Names:F20M17.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G31880
TAIRilocus:2045228 AT2G31880

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 284ExtracellularSequence analysisAdd BLAST253
Transmembranei285 – 305HelicalSequence analysisAdd BLAST21
Topological domaini306 – 641CytoplasmicSequence analysisAdd BLAST336

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Suppresses BIR1 (bir1-1) disruption phenotype. When associated with AGD5/NEV disruption, premature shedding of floral organs and enlarge abscission zones.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi129V → M in sobir1-8; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi329S → N in sobir1-4; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi356G → R in sobir1-7; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi377K → E: Loss of kinase activity. 1 Publication1
Mutagenesisi407E → K in evr-2; loss of kinase activity. 1 Publication1
Mutagenesisi417R → W in sobir1-9; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi455N → D in sobir1-10; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi557G → R in sobir1-5; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi575E → K in sobir1-2; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi626R → K in sobir1-6; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000040335532 – 641Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1Add BLAST610

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi154N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi186N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi256N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Autophosphorylated on Ser, Thr and Tyr residues.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SKB2
PRIDEiQ9SKB2

PTM databases

iPTMnetiQ9SKB2
SwissPalmiQ9SKB2

Expressioni

Tissue specificityi

Mostly present in leaves and flowers, with increasing expression in older flowers.1 Publication

Developmental stagei

Expressed in floral organ abscission zones (AZs) prior to cell separation and subsequent shedding. Also present within the style of developing fruits, at the bases of cauline leaves, and in the stems of the first rosette leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ9SKB2 baseline and differential
GenevisibleiQ9SKB2 AT

Interactioni

Subunit structurei

Interacts with CST (PubMed:21628627). Interacts with RLP23 (PubMed:24525519, PubMed:27251392). Component of a trimeric complex composed of RLP23, SOBIR1 and BAK1. BAK1 is recruited into a pre-formed RLP23-SOBIR1 complex in a ligand-dependent manner (PubMed:27251392).3 Publications

Protein-protein interaction databases

BioGridi3093, 31 interactors
STRINGi3702.AT2G31880.1

Structurei

3D structure databases

ProteinModelPortaliQ9SKB2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati112 – 133LRR 1Add BLAST22
Repeati136 – 159LRR 2Add BLAST24
Repeati160 – 182LRR 3Add BLAST23
Repeati183 – 205LRR 4Add BLAST23
Repeati207 – 228LRR 5Add BLAST22
Domaini347 – 641Protein kinasePROSITE-ProRule annotationAdd BLAST295

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi261 – 283Lys-richAdd BLAST23

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IH4C Eukaryota
COG0515 LUCA
HOGENOMiHOG000071072
InParanoidiQ9SKB2
OMAiVSSSTEW
OrthoDBiEOG09360DPL
PhylomeDBiQ9SKB2

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR001611 Leu-rich_rpt
IPR032675 LRR_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF13855 LRR_8, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 4 hits
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SKB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPTGSANL FLRPLILAVL SFLLLSSFVS SVEWLDIDSS DLKALQVIET
60 70 80 90 100
ELGVNSQRSS ASDVNPCGRR GVFCERRHSA TTGEYVLRVT RLVYRSRSLT
110 120 130 140 150
GTISPVIGML SELKELTLSN NQLVNAVPVD ILSCKQLEVL DLRKNRFSGQ
160 170 180 190 200
IPGNFSSLSR LRILDLSSNK LSGNLNFLKN LRNLENLSVA NNLFSGKIPE
210 220 230 240 250
QIVSFHNLRF FDFSGNRYLE GPAPVMSSIK LQTSPHQTRH ILAETPTSSP
260 270 280 290 300
TNKPNNSTTS KAPKGAPKPG KLKKKKKKSK KKKVAAWILG FVVGAIGGTI
310 320 330 340 350
SGFVFSVLFK LIIQAIRGSE KPPGPSIFSP LIKKAEDLAF LENEEALASL
360 370 380 390 400
EIIGRGGCGE VFKAELPGSN GKIIAVKKVI QPPKDADELT DEDSKFLNKK
410 420 430 440 450
MRQIRSEINT VGHIRHRNLL PLLAHVSRPE CHYLVYEYME KGSLQDILTD
460 470 480 490 500
VQAGNQELMW PARHKIALGI AAGLEYLHMD HNPRIIHRDL KPANVLLDDD
510 520 530 540 550
MEARISDFGL AKAMPDAVTH ITTSHVAGTV GYIAPEFYQT HKFTDKCDIY
560 570 580 590 600
SFGVILGILV IGKLPSDEFF QHTDEMSLIK WMRNIITSEN PSLAIDPKLM
610 620 630 640
DQGFDEQMLL VLKIACYCTL DDPKQRPNSK DVRTMLSQIK H
Length:641
Mass (Da):71,111
Last modified:May 1, 2000 - v1
Checksum:iC061139D4B52681C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112E → V in AAL25569 (PubMed:14593172).Curated1
Sequence conflicti490L → S in AAL25569 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006533 Genomic DNA Translation: AAD32284.1
CP002685 Genomic DNA Translation: AEC08599.1
AF370596 mRNA Translation: AAK43915.1
AY058153 mRNA Translation: AAL25569.1
FJ708707 mRNA Translation: ACN59302.1
PIRiC84726
RefSeqiNP_180747.1, NM_128746.3
UniGeneiAt.13945
At.75587

Genome annotation databases

EnsemblPlantsiAT2G31880.1; AT2G31880.1; AT2G31880
GeneIDi817746
GrameneiAT2G31880.1; AT2G31880.1; AT2G31880
KEGGiath:AT2G31880

Similar proteinsi

Entry informationi

Entry nameiSBIR1_ARATH
AccessioniPrimary (citable) accession number: Q9SKB2
Secondary accession number(s): Q93Z40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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