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Protein

Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1

Gene

SOBIR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Promotes the activation of plant defense and cell death. Functions as an inhibitor/regulator of abscission, probably by regulating membrane trafficking during abscission.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei377ATPPROSITE-ProRule annotation1
Active sitei489Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi353 – 361ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB
  • protein tyrosine kinase activity Source: UniProtKB
  • transmembrane receptor protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • positive regulation of cell death Source: TAIR
  • positive regulation of defense response Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1 (EC:2.7.10.1, EC:2.7.11.1)
Alternative name(s):
Protein EVERSHED
Protein SUPPRESSOR OF BIR1-1
Gene namesi
Name:SOBIR1
Synonyms:EVR
Ordered Locus Names:At2g31880
ORF Names:F20M17.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G31880.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 284ExtracellularSequence analysisAdd BLAST253
Transmembranei285 – 305HelicalSequence analysisAdd BLAST21
Topological domaini306 – 641CytoplasmicSequence analysisAdd BLAST336

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Suppresses BIR1 (bir1-1) disruption phenotype. When associated with AGD5/NEV disruption, premature shedding of floral organs and enlarge abscission zones.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi129V → M in sobir1-8; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi329S → N in sobir1-4; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi356G → R in sobir1-7; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi377K → E: Loss of kinase activity. 1 Publication1
Mutagenesisi407E → K in evr-2; loss of kinase activity. 1 Publication1
Mutagenesisi417R → W in sobir1-9; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi455N → D in sobir1-10; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi557G → R in sobir1-5; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi575E → K in sobir1-2; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1
Mutagenesisi626R → K in sobir1-6; suppresses BIR1 (bir1-1) disruption phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000040335532 – 641Leucine-rich repeat receptor-like serine/threonine/tyrosine-protein kinase SOBIR1Add BLAST610

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi154N-linked (GlcNAc...)Sequence analysis1
Glycosylationi186N-linked (GlcNAc...)Sequence analysis1
Glycosylationi256N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Autophosphorylated on Ser, Thr and Tyr residues.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SKB2.
PRIDEiQ9SKB2.

PTM databases

iPTMnetiQ9SKB2.
SwissPalmiQ9SKB2.

Expressioni

Tissue specificityi

Mostly present in leaves and flowers, with increasing expression in older flowers.1 Publication

Developmental stagei

Expressed in floral organ abscission zones (AZs) prior to cell separation and subsequent shedding. Also present within the style of developing fruits, at the bases of cauline leaves, and in the stems of the first rosette leaves.1 Publication

Gene expression databases

GenevisibleiQ9SKB2. AT.

Interactioni

Subunit structurei

Interacts with CST.1 Publication

Protein-protein interaction databases

BioGridi3093. 31 interactors.
STRINGi3702.AT2G31880.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SKB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati112 – 133LRR 1Add BLAST22
Repeati136 – 159LRR 2Add BLAST24
Repeati160 – 182LRR 3Add BLAST23
Repeati183 – 205LRR 4Add BLAST23
Repeati207 – 228LRR 5Add BLAST22
Domaini347 – 641Protein kinasePROSITE-ProRule annotationAdd BLAST295

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi261 – 283Lys-richAdd BLAST23

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 5 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IH4C. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000071072.
InParanoidiQ9SKB2.
OMAiQHTDEMS.
OrthoDBiEOG09360DPL.
PhylomeDBiQ9SKB2.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13855. LRR_8. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SKB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPTGSANL FLRPLILAVL SFLLLSSFVS SVEWLDIDSS DLKALQVIET
60 70 80 90 100
ELGVNSQRSS ASDVNPCGRR GVFCERRHSA TTGEYVLRVT RLVYRSRSLT
110 120 130 140 150
GTISPVIGML SELKELTLSN NQLVNAVPVD ILSCKQLEVL DLRKNRFSGQ
160 170 180 190 200
IPGNFSSLSR LRILDLSSNK LSGNLNFLKN LRNLENLSVA NNLFSGKIPE
210 220 230 240 250
QIVSFHNLRF FDFSGNRYLE GPAPVMSSIK LQTSPHQTRH ILAETPTSSP
260 270 280 290 300
TNKPNNSTTS KAPKGAPKPG KLKKKKKKSK KKKVAAWILG FVVGAIGGTI
310 320 330 340 350
SGFVFSVLFK LIIQAIRGSE KPPGPSIFSP LIKKAEDLAF LENEEALASL
360 370 380 390 400
EIIGRGGCGE VFKAELPGSN GKIIAVKKVI QPPKDADELT DEDSKFLNKK
410 420 430 440 450
MRQIRSEINT VGHIRHRNLL PLLAHVSRPE CHYLVYEYME KGSLQDILTD
460 470 480 490 500
VQAGNQELMW PARHKIALGI AAGLEYLHMD HNPRIIHRDL KPANVLLDDD
510 520 530 540 550
MEARISDFGL AKAMPDAVTH ITTSHVAGTV GYIAPEFYQT HKFTDKCDIY
560 570 580 590 600
SFGVILGILV IGKLPSDEFF QHTDEMSLIK WMRNIITSEN PSLAIDPKLM
610 620 630 640
DQGFDEQMLL VLKIACYCTL DDPKQRPNSK DVRTMLSQIK H
Length:641
Mass (Da):71,111
Last modified:May 1, 2000 - v1
Checksum:iC061139D4B52681C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112E → V in AAL25569 (PubMed:14593172).Curated1
Sequence conflicti490L → S in AAL25569 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006533 Genomic DNA. Translation: AAD32284.1.
CP002685 Genomic DNA. Translation: AEC08599.1.
AF370596 mRNA. Translation: AAK43915.1.
AY058153 mRNA. Translation: AAL25569.1.
FJ708707 mRNA. Translation: ACN59302.1.
PIRiC84726.
RefSeqiNP_180747.1. NM_128746.3.
UniGeneiAt.13945.
At.75587.

Genome annotation databases

EnsemblPlantsiAT2G31880.1; AT2G31880.1; AT2G31880.
GeneIDi817746.
GrameneiAT2G31880.1; AT2G31880.1; AT2G31880.
KEGGiath:AT2G31880.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006533 Genomic DNA. Translation: AAD32284.1.
CP002685 Genomic DNA. Translation: AEC08599.1.
AF370596 mRNA. Translation: AAK43915.1.
AY058153 mRNA. Translation: AAL25569.1.
FJ708707 mRNA. Translation: ACN59302.1.
PIRiC84726.
RefSeqiNP_180747.1. NM_128746.3.
UniGeneiAt.13945.
At.75587.

3D structure databases

ProteinModelPortaliQ9SKB2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3093. 31 interactors.
STRINGi3702.AT2G31880.1.

PTM databases

iPTMnetiQ9SKB2.
SwissPalmiQ9SKB2.

Proteomic databases

PaxDbiQ9SKB2.
PRIDEiQ9SKB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G31880.1; AT2G31880.1; AT2G31880.
GeneIDi817746.
GrameneiAT2G31880.1; AT2G31880.1; AT2G31880.
KEGGiath:AT2G31880.

Organism-specific databases

TAIRiAT2G31880.

Phylogenomic databases

eggNOGiENOG410IH4C. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000071072.
InParanoidiQ9SKB2.
OMAiQHTDEMS.
OrthoDBiEOG09360DPL.
PhylomeDBiQ9SKB2.

Miscellaneous databases

PROiQ9SKB2.

Gene expression databases

GenevisibleiQ9SKB2. AT.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13855. LRR_8. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSBIR1_ARATH
AccessioniPrimary (citable) accession number: Q9SKB2
Secondary accession number(s): Q93Z40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.