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Protein

Mitochondrial uncoupling protein 5

Gene

PUMP5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. May be involved in protecting plant cells against oxidative stress damage. Recombinant PUMP5, reconstituted into liposomes, transports a wide range of dicarboxylic acids including malate, oxaloacetate and succinate as well as phosphate, sulfate and thiosulfate. However, it is unknown if these transports are of any biological significance in vivo.2 Publications

GO - Molecular functioni

  • dicarboxylic acid transmembrane transporter activity Source: TAIR
  • oxidative phosphorylation uncoupler activity Source: UniProtKB

GO - Biological processi

  • mitochondrial transport Source: TAIR
  • proton transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Stress response, Transport

Enzyme and pathway databases

ReactomeiR-ATH-1614517. Sulfide oxidation to sulfate.
R-ATH-428643. Organic anion transporters.
R-ATH-70263. Gluconeogenesis.

Protein family/group databases

TCDBi2.A.29.2.9. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial uncoupling protein 5
Short name:
AtPUMP5
Alternative name(s):
Mitochondrial dicarboxylate carrier 1
Gene namesi
Name:PUMP5
Synonyms:DIC1, UCP5
Ordered Locus Names:At2g22500
ORF Names:F14M13.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G22500.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 26Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei77 – 97Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei123 – 143Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei182 – 202Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei223 – 243Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei280 – 300Helical; Name=6Sequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004202591 – 313Mitochondrial uncoupling protein 5Add BLAST313

Proteomic databases

PaxDbiQ9SJY5.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and flowers.2 Publications

Inductioni

By cold stress and high light.2 Publications

Gene expression databases

GenevisibleiQ9SJY5. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G22500.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SJY5.
SMRiQ9SJY5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 108Solcar 1Add BLAST105
Repeati117 – 208Solcar 2Add BLAST92
Repeati217 – 307Solcar 3Add BLAST91

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0759. Eukaryota.
ENOG410XQHU. LUCA.
InParanoidiQ9SJY5.
KOiK15104.
OMAiETKTMPL.
OrthoDBiEOG09360KFV.
PhylomeDBiQ9SJY5.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SJY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLKGFAEGG IASIVAGCST HPLDLIKVRM QLQGESAPIQ TNLRPALAFQ
60 70 80 90 100
TSTTVNAPPL RVGVIGVGSR LIREEGMRAL FSGVSATVLR QTLYSTTRMG
110 120 130 140 150
LYDIIKGEWT DPETKTMPLM KKIGAGAIAG AIGAAVGNPA DVAMVRMQAD
160 170 180 190 200
GRLPLTDRRN YKSVLDAITQ MIRGEGVTSL WRGSSLTINR AMLVTSSQLA
210 220 230 240 250
SYDSVKETIL EKGLLKDGLG THVSASFAAG FVASVASNPV DVIKTRVMNM
260 270 280 290 300
KVVAGVAPPY KGAVDCALKT VKAEGIMSLY KGFIPTVSRQ APFTVVLFVT
310
LEQVKKLFKD YDF
Length:313
Mass (Da):33,357
Last modified:May 1, 2000 - v1
Checksum:i447903EAA3865E6C
GO

Sequence cautioni

The sequence BAF02125 differs from that shown. Reason: Frameshift at position 257.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130G → V in AAK44155 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM236862 mRNA. Translation: CAJ86454.1.
AC006592 Genomic DNA. Translation: AAD22351.1.
CP002685 Genomic DNA. Translation: AEC07315.1.
AF370340 mRNA. Translation: AAK44155.1.
AF370588 mRNA. Translation: AAK43907.1.
AY142648 mRNA. Translation: AAN13106.1.
AK230325 mRNA. Translation: BAF02125.1. Frameshift.
PIRiD84613.
RefSeqiNP_179836.1. NM_127816.3.
UniGeneiAt.20524.
At.34671.

Genome annotation databases

EnsemblPlantsiAT2G22500.1; AT2G22500.1; AT2G22500.
GeneIDi816783.
GrameneiAT2G22500.1; AT2G22500.1; AT2G22500.
KEGGiath:AT2G22500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM236862 mRNA. Translation: CAJ86454.1.
AC006592 Genomic DNA. Translation: AAD22351.1.
CP002685 Genomic DNA. Translation: AEC07315.1.
AF370340 mRNA. Translation: AAK44155.1.
AF370588 mRNA. Translation: AAK43907.1.
AY142648 mRNA. Translation: AAN13106.1.
AK230325 mRNA. Translation: BAF02125.1. Frameshift.
PIRiD84613.
RefSeqiNP_179836.1. NM_127816.3.
UniGeneiAt.20524.
At.34671.

3D structure databases

ProteinModelPortaliQ9SJY5.
SMRiQ9SJY5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G22500.1.

Protein family/group databases

TCDBi2.A.29.2.9. the mitochondrial carrier (mc) family.

Proteomic databases

PaxDbiQ9SJY5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G22500.1; AT2G22500.1; AT2G22500.
GeneIDi816783.
GrameneiAT2G22500.1; AT2G22500.1; AT2G22500.
KEGGiath:AT2G22500.

Organism-specific databases

TAIRiAT2G22500.

Phylogenomic databases

eggNOGiKOG0759. Eukaryota.
ENOG410XQHU. LUCA.
InParanoidiQ9SJY5.
KOiK15104.
OMAiETKTMPL.
OrthoDBiEOG09360KFV.
PhylomeDBiQ9SJY5.

Enzyme and pathway databases

ReactomeiR-ATH-1614517. Sulfide oxidation to sulfate.
R-ATH-428643. Organic anion transporters.
R-ATH-70263. Gluconeogenesis.

Miscellaneous databases

PROiQ9SJY5.

Gene expression databases

GenevisibleiQ9SJY5. AT.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUMP5_ARATH
AccessioniPrimary (citable) accession number: Q9SJY5
Secondary accession number(s): Q0WL82, Q94K32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.