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Protein

Probable glutamyl-tRNA reductase 3, chloroplastic

Gene

HEMA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).By similarity

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA (By similarity).By similarity

Catalytic activityi

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (AXX17_At1g09980), Glutamyl-tRNA reductase 2, chloroplastic (HEMA2), Glutamyl-tRNA reductase 1, chloroplastic (HEMA1), Glutamyl-tRNA reductase (AXX17_At2g27450), Glutamyl-tRNA reductase (AXX17_At1g52270), Probable glutamyl-tRNA reductase 3, chloroplastic (HEMA3)
  2. Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic (GSA1), Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic (GSA2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei130NucleophileBy similarity1
Sitei179Important for activityBy similarity1
Binding sitei189SubstrateBy similarity1
Binding sitei200SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi269 – 274NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processChlorophyll biosynthesis, Porphyrin biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciARA:AT2G31250-MONOMER
UniPathwayiUPA00251; UER00316
UPA00668

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamyl-tRNA reductase 3, chloroplastic (EC:1.2.1.70)
Gene namesi
Name:HEMA3
Ordered Locus Names:At2g31250
ORF Names:F16D14.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G31250
TAIRilocus:2042516 AT2G31250

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52ChloroplastSequence analysisAdd BLAST52
ChainiPRO_000042267153 – 524Probable glutamyl-tRNA reductase 3, chloroplasticAdd BLAST472

Proteomic databases

PaxDbiQ9SJX1
PRIDEiQ9SJX1

PTM databases

iPTMnetiQ9SJX1

Expressioni

Gene expression databases

ExpressionAtlasiQ9SJX1 baseline and differential
GenevisibleiQ9SJX1 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G31250.1

Structurei

3D structure databases

ProteinModelPortaliQ9SJX1
SMRiQ9SJX1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 132Substrate bindingBy similarity4
Regioni194 – 196Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glutamyl-tRNA reductase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0373 LUCA
HOGENOMiHOG000109651
InParanoidiQ9SJX1
KOiK02492
OMAiHTAPLEM
OrthoDBiEOG093609MY
PhylomeDBiQ9SJX1

Family and domain databases

Gene3Di3.30.460.30, 1 hit
HAMAPiMF_00087 Glu_tRNA_reductase, 1 hit
InterProiView protein in InterPro
IPR000343 4pyrrol_synth_GluRdtase
IPR015896 4pyrrol_synth_GluRdtase_dimer
IPR015895 4pyrrol_synth_GluRdtase_N
IPR018214 GluRdtase_CS
IPR036453 GluRdtase_dimer_dom_sf
IPR036343 GluRdtase_N_sf
IPR036291 NAD(P)-bd_dom_sf
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF00745 GlutR_dimer, 1 hit
PF05201 GlutR_N, 1 hit
PF01488 Shikimate_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF69075 SSF69075, 1 hit
SSF69742 SSF69742, 1 hit
TIGRFAMsiTIGR01035 hemA, 1 hit
PROSITEiView protein in PROSITE
PS00747 GLUTR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SJX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSNASVVL SPNLETSSSW YHHNPSSSLD LIRIHTLPMN KMTRRGLIQR
60 70 80 90 100
VRCETSPSSD SSPLDKLKNS PAIDRYTRER SSIVVIGLSF HTSPLEMRER
110 120 130 140 150
LAIPEAEWPL AITQLCALNH IEEAAVLSTC NRIEIYVSAL SRYRGVKEVT
160 170 180 190 200
EWMSKRSGIP VSDICQHRFL LYNKDATQHL FQVSAGLESL VIGENQIQSQ
210 220 230 240 250
VRKAEQVVKQ EGFGRIISTL FEKANKAGKR VRAQTNIASG AVSVSSAAVE
260 270 280 290 300
LALTKLPGSV SSAMMLVIGA GEMGKRIIEH LVAKGCTKMV VMNRSEDKVA
310 320 330 340 350
AIRKEMQSGV EIIYKPLDEI LACAAEANVI FTSTSSETPL FLKEHVEILP
360 370 380 390 400
PCPADYARLF VDISVPRNVG SCVAELDSAR VYNVDDLKEV VAANKEDRAR
410 420 430 440 450
KSMEALPIIR EETIEFEGWR DSLQTFPTIR KLRSKTERIR AECVEKLISK
460 470 480 490 500
HGNGMDKKTR EAVEKQTRII VNNILDYPMK HLRYDGTGSS KLRETLENMQ
510 520
AVNRIYELDG ELLEEKIREK KDKK
Length:524
Mass (Da):58,691
Last modified:May 1, 2000 - v1
Checksum:iE1DBE6D164EE1957
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006593 Genomic DNA Translation: AAD20670.1
CP002685 Genomic DNA Translation: AEC08514.1
PIRiD84718
RefSeqiNP_180683.1, NM_128681.3
UniGeneiAt.38245

Genome annotation databases

EnsemblPlantsiAT2G31250.1; AT2G31250.1; AT2G31250
GeneIDi817682
GrameneiAT2G31250.1; AT2G31250.1; AT2G31250
KEGGiath:AT2G31250

Similar proteinsi

Entry informationi

Entry nameiHEM13_ARATH
AccessioniPrimary (citable) accession number: Q9SJX1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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