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Protein

Probable glutamyl-tRNA reductase 3, chloroplastic

Gene

HEMA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).By similarity

Catalytic activityi

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (AXX17_At1g09980), Glutamyl-tRNA reductase 2, chloroplastic (HEMA2), Glutamyl-tRNA reductase 1, chloroplastic (HEMA1), Glutamyl-tRNA reductase (AXX17_At2g27450), Glutamyl-tRNA reductase (AXX17_At1g52270), Probable glutamyl-tRNA reductase 3, chloroplastic (HEMA3)
  2. Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic (GSA1), Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic (GSA2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei130NucleophileBy similarity1
Sitei179Important for activityBy similarity1
Binding sitei189SubstrateBy similarity1
Binding sitei200SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi269 – 274NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT2G31250-MONOMER.
UniPathwayiUPA00251; UER00316.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamyl-tRNA reductase 3, chloroplastic (EC:1.2.1.70)
Gene namesi
Name:HEMA3
Ordered Locus Names:At2g31250
ORF Names:F16D14.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G31250.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52ChloroplastSequence analysisAdd BLAST52
ChainiPRO_000042267153 – 524Probable glutamyl-tRNA reductase 3, chloroplasticAdd BLAST472

Proteomic databases

PaxDbiQ9SJX1.
PRIDEiQ9SJX1.

PTM databases

iPTMnetiQ9SJX1.

Expressioni

Gene expression databases

GenevisibleiQ9SJX1. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G31250.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SJX1.
SMRiQ9SJX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 132Substrate bindingBy similarity4
Regioni194 – 196Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glutamyl-tRNA reductase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0373. LUCA.
HOGENOMiHOG000109651.
InParanoidiQ9SJX1.
KOiK02492.
OMAiSSAMMLV.
OrthoDBiEOG093609MY.
PhylomeDBiQ9SJX1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00087. Glu_tRNA_reductase. 1 hit.
InterProiIPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR016040. NAD(P)-bd_dom.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsiTIGR01035. hemA. 1 hit.
PROSITEiPS00747. GLUTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SJX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSNASVVL SPNLETSSSW YHHNPSSSLD LIRIHTLPMN KMTRRGLIQR
60 70 80 90 100
VRCETSPSSD SSPLDKLKNS PAIDRYTRER SSIVVIGLSF HTSPLEMRER
110 120 130 140 150
LAIPEAEWPL AITQLCALNH IEEAAVLSTC NRIEIYVSAL SRYRGVKEVT
160 170 180 190 200
EWMSKRSGIP VSDICQHRFL LYNKDATQHL FQVSAGLESL VIGENQIQSQ
210 220 230 240 250
VRKAEQVVKQ EGFGRIISTL FEKANKAGKR VRAQTNIASG AVSVSSAAVE
260 270 280 290 300
LALTKLPGSV SSAMMLVIGA GEMGKRIIEH LVAKGCTKMV VMNRSEDKVA
310 320 330 340 350
AIRKEMQSGV EIIYKPLDEI LACAAEANVI FTSTSSETPL FLKEHVEILP
360 370 380 390 400
PCPADYARLF VDISVPRNVG SCVAELDSAR VYNVDDLKEV VAANKEDRAR
410 420 430 440 450
KSMEALPIIR EETIEFEGWR DSLQTFPTIR KLRSKTERIR AECVEKLISK
460 470 480 490 500
HGNGMDKKTR EAVEKQTRII VNNILDYPMK HLRYDGTGSS KLRETLENMQ
510 520
AVNRIYELDG ELLEEKIREK KDKK
Length:524
Mass (Da):58,691
Last modified:May 1, 2000 - v1
Checksum:iE1DBE6D164EE1957
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006593 Genomic DNA. Translation: AAD20670.1.
CP002685 Genomic DNA. Translation: AEC08514.1.
PIRiD84718.
RefSeqiNP_180683.1. NM_128681.3.
UniGeneiAt.38245.

Genome annotation databases

EnsemblPlantsiAT2G31250.1; AT2G31250.1; AT2G31250.
GeneIDi817682.
GrameneiAT2G31250.1; AT2G31250.1; AT2G31250.
KEGGiath:AT2G31250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006593 Genomic DNA. Translation: AAD20670.1.
CP002685 Genomic DNA. Translation: AEC08514.1.
PIRiD84718.
RefSeqiNP_180683.1. NM_128681.3.
UniGeneiAt.38245.

3D structure databases

ProteinModelPortaliQ9SJX1.
SMRiQ9SJX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G31250.1.

PTM databases

iPTMnetiQ9SJX1.

Proteomic databases

PaxDbiQ9SJX1.
PRIDEiQ9SJX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G31250.1; AT2G31250.1; AT2G31250.
GeneIDi817682.
GrameneiAT2G31250.1; AT2G31250.1; AT2G31250.
KEGGiath:AT2G31250.

Organism-specific databases

TAIRiAT2G31250.

Phylogenomic databases

eggNOGiCOG0373. LUCA.
HOGENOMiHOG000109651.
InParanoidiQ9SJX1.
KOiK02492.
OMAiSSAMMLV.
OrthoDBiEOG093609MY.
PhylomeDBiQ9SJX1.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00316.
UPA00668.
BioCyciARA:AT2G31250-MONOMER.

Miscellaneous databases

PROiQ9SJX1.

Gene expression databases

GenevisibleiQ9SJX1. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00087. Glu_tRNA_reductase. 1 hit.
InterProiIPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR016040. NAD(P)-bd_dom.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsiTIGR01035. hemA. 1 hit.
PROSITEiPS00747. GLUTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM13_ARATH
AccessioniPrimary (citable) accession number: Q9SJX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.