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Protein

Sec-independent protein translocase protein TATC, chloroplastic

Gene

TATC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across the thylakoid membrane. Involved in delta pH-dependent protein transport required for chloroplast development, especially thylakoid membrane formation. TATC and TATB mediate precursor recognition, whereas TATA facilitates translocation.1 Publication

GO - Molecular functioni

  • proton motive force dependent protein transmembrane transporter activity Source: TAIR

GO - Biological processi

  • double fertilization forming a zygote and endosperm Source: TAIR
  • protein transmembrane transport Source: GOC
  • thylakoid membrane organization Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Protein family/group databases

TCDBi2.A.64.2.1. the twin arginine targeting (tat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sec-independent protein translocase protein TATC, chloroplastic
Alternative name(s):
Protein ALBINO AND PALE GREEN 2
Protein TWIN-ARGININE TRANSLOCATION C
Protein UNFERTILIZED EMBRYO SAC 3
Gene namesi
Name:TATC
Synonyms:APG2, UNE3
Ordered Locus Names:At2g01110
ORF Names:F23H14.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01110.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 12799StromalSequence analysisAdd
BLAST
Transmembranei128 – 14821HelicalSequence analysisAdd
BLAST
Topological domaini149 – 17931LumenalSequence analysisAdd
BLAST
Transmembranei180 – 20021HelicalSequence analysisAdd
BLAST
Topological domaini201 – 2099StromalSequence analysis
Transmembranei210 – 23021HelicalSequence analysisAdd
BLAST
Topological domaini231 – 25727LumenalSequence analysisAdd
BLAST
Transmembranei258 – 27821HelicalSequence analysisAdd
BLAST
Topological domaini279 – 28911StromalSequence analysisAdd
BLAST
Transmembranei290 – 31021HelicalSequence analysisAdd
BLAST
Topological domaini311 – 3144LumenalSequence analysis
Transmembranei315 – 33521HelicalSequence analysisAdd
BLAST
Topological domaini336 – 3405StromalSequence analysis

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • integral component of thylakoid membrane Source: TAIR
  • receptor complex Source: TAIR
  • TAT protein transport complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

Seedling lethality when homozygous. When grown on agar medium, mutant seedlings have an albino phenotype and contain plastid lacking internal membrane structures.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 340Sec-independent protein translocase protein TATC, chloroplasticPRO_0000419910
Transit peptidei1 – 2828ChloroplastSequence analysisAdd
BLAST
Transit peptidei29 – ?ThylakoidSequence analysis

Proteomic databases

PaxDbiQ9SJV5.
PRIDEiQ9SJV5.

Expressioni

Gene expression databases

GenevisibleiQ9SJV5. AT.

Interactioni

Subunit structurei

In thylakoid membranes, TATC and TATB form a large receptor complex, containing about eight TATC-TATB pairs, which binds the precursor protein. Twin arginine signal peptide promotes pH-triggered docking of TATA oligomers to TATC-TATB receptor complex, inducing a conformational switch of TATA that results in activation of the translocase. TATA dissociates from TATC-TATB upon completion of translocation.1 Publication

Protein-protein interaction databases

STRINGi3702.AT2G01110.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SJV5.
SMRiQ9SJV5. Positions 115-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TatC family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIPA. Eukaryota.
COG0805. LUCA.
HOGENOMiHOG000245380.
InParanoidiQ9SJV5.
KOiK03118.
OMAiVEQAWSI.
PhylomeDBiQ9SJV5.

Family and domain databases

HAMAPiMF_00902. TatC.
InterProiIPR019820. Sec-indep_translocase_CS.
IPR002033. TatC.
[Graphical view]
PfamiPF00902. TatC. 1 hit.
[Graphical view]
PRINTSiPR01840. TATCFAMILY.
TIGRFAMsiTIGR00945. tatC. 1 hit.
PROSITEiPS01218. TATC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SJV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTSTSSAL IHHFRLTTRN LGSPTKQRCP YAVTFCNSWR EAGLRYSVTQ
60 70 80 90 100
RRSKGFGPVS ALNDDDSPTE TTPGVGSAVE DRPPDSSEDR SSSVYEFLYP
110 120 130 140 150
RKEELPDDKE MTIFDHLEEL RERIFVSVLA VGAAILGCFA FSKDLIVFLE
160 170 180 190 200
APVKTQGVRF LQLAPGEFFF TTLKVSGYCG LLLGSPVILY EIIAFVLPGL
210 220 230 240 250
TRAERRFLGP IVFGSSLLFY AGLAFSYWVL TPAALNFFVN YAEGVVESLW
260 270 280 290 300
SIDQYFEFVL VLMFSTGLSF QVPVIQLLLG QVGVVSGDQM LSIWRYVVVG
310 320 330 340
AVVAAAVVTP STDPVTQMLL ATPLLGLYLG GAWMVKLTGR
Length:340
Mass (Da):37,367
Last modified:June 1, 2002 - v2
Checksum:i1429C265BFEDB281
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB054096 mRNA. Translation: BAB62074.1.
AF145045 mRNA. Translation: AAD33946.1.
AC006837 Genomic DNA. Translation: AAF18659.2.
CP002685 Genomic DNA. Translation: AEC05402.1.
AF360172 mRNA. Translation: AAK25882.1.
AY056347 mRNA. Translation: AAL07196.1.
AY084784 mRNA. Translation: AAM61351.1.
PIRiT44583.
RefSeqiNP_565252.1. NM_126173.3.
UniGeneiAt.11297.

Genome annotation databases

EnsemblPlantsiAT2G01110.1; AT2G01110.1; AT2G01110.
GeneIDi814640.
GrameneiAT2G01110.1; AT2G01110.1; AT2G01110.
KEGGiath:AT2G01110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB054096 mRNA. Translation: BAB62074.1.
AF145045 mRNA. Translation: AAD33946.1.
AC006837 Genomic DNA. Translation: AAF18659.2.
CP002685 Genomic DNA. Translation: AEC05402.1.
AF360172 mRNA. Translation: AAK25882.1.
AY056347 mRNA. Translation: AAL07196.1.
AY084784 mRNA. Translation: AAM61351.1.
PIRiT44583.
RefSeqiNP_565252.1. NM_126173.3.
UniGeneiAt.11297.

3D structure databases

ProteinModelPortaliQ9SJV5.
SMRiQ9SJV5. Positions 115-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G01110.1.

Protein family/group databases

TCDBi2.A.64.2.1. the twin arginine targeting (tat) family.

Proteomic databases

PaxDbiQ9SJV5.
PRIDEiQ9SJV5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01110.1; AT2G01110.1; AT2G01110.
GeneIDi814640.
GrameneiAT2G01110.1; AT2G01110.1; AT2G01110.
KEGGiath:AT2G01110.

Organism-specific databases

TAIRiAT2G01110.

Phylogenomic databases

eggNOGiENOG410IIPA. Eukaryota.
COG0805. LUCA.
HOGENOMiHOG000245380.
InParanoidiQ9SJV5.
KOiK03118.
OMAiVEQAWSI.
PhylomeDBiQ9SJV5.

Miscellaneous databases

PROiQ9SJV5.

Gene expression databases

GenevisibleiQ9SJV5. AT.

Family and domain databases

HAMAPiMF_00902. TatC.
InterProiIPR019820. Sec-indep_translocase_CS.
IPR002033. TatC.
[Graphical view]
PfamiPF00902. TatC. 1 hit.
[Graphical view]
PRINTSiPR01840. TATCFAMILY.
TIGRFAMsiTIGR00945. tatC. 1 hit.
PROSITEiPS01218. TATC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An essential role of a TatC homologue of a Delta pH- dependent protein transporter in thylakoid membrane formation during chloroplast development in Arabidopsis thaliana."
    Motohashi R., Nagata N., Ito T., Takahashi S., Hobo T., Yoshida S., Shinozaki K.
    Proc. Natl. Acad. Sci. U.S.A. 98:10499-10504(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  2. "An Arabidopsis homolog of TatC/YCF43."
    Summer E.J., McCaffery M.W., Cline K.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Tat subunit stoichiometry in Arabidopsis thaliana challenges the proposed function of TatA as the translocation pore."
    Jakob M., Kaiser S., Gutensohn M., Hanner P., Kloesgen R.B.
    Biochim. Biophys. Acta 1793:388-394(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiTATC_ARATH
AccessioniPrimary (citable) accession number: Q9SJV5
Secondary accession number(s): Q9XFI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: June 1, 2002
Last modified: February 17, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

According to PubMed:18930082, TATA is detectable only in minor amounts in Arabidopsis chloroplasts.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.