Probable fructose-bisphosphate aldolase 1, chloroplastic precursor

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Q9SJU4 (ALFC1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable fructose-bisphosphate aldolase 1, chloroplastic
EC=4.1.2.13

Gene names

Name:	FBA1
Ordered Locus Names:	At2g21330
ORF Names:	F3K23.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subcellular location	Plastid › chloroplast › plastoglobule Ref.4 Ref.5.
Post-translational modification	Can be trimethylated at Lys-395 by LSMT-L, but the trimethylation has no effect in vitro on the kinetic properties of the enzyme. Ref.6
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway response to cadmium ion Inferred from expression pattern PubMed 16502469. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR chloroplast envelope Inferred from direct assay PubMed 12938931. Source: TAIR chloroplast thylakoid Inferred from direct assay PubMed 20061580. Source: TAIR cytosolic ribosome Inferred from direct assay PubMed 15821981. Source: TAIR plastoglobule Inferred from direct assay Ref.4 Ref.5. Source: TAIR thylakoid lumen Inferred from direct assay PubMed 11719511. Source: TAIR
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SJU4-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 10

Chloroplast Potential

Chain

11 – 399

389

Probable fructose-bisphosphate aldolase 1, chloroplastic

PRO_0000286526

Sites

Active site

226

Proton acceptor By similarity

Active site

268

Schiff-base intermediate with dihydroxyacetone-P By similarity

Binding site

Substrate By similarity

Binding site

186

Substrate By similarity

Site

399

Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Amino acid modifications

Modified residue

395

N6,N6,N6-trimethyllysine Ref.6

Experimental info

Sequence conflict

236

G → S in AAK59548. Ref.3

Sequence conflict

315

E → G in AAK59548. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 26CC71FA3C571B53
Show »

FASTA

399

42,931

        10         20         30         40         50         60 
MASSTATMLK ASPVKSDWVK GQSLLLRQPS SVSAIRSHVA PSALTVRAAS AYADELVKTA 

        70         80         90        100        110        120 
KTIASPGHGI MAMDESNATC GKRLASIGLE NTEANRQAYR TLLVSAPGLG QYISGAILFE 

       130        140        150        160        170        180 
ETLYQSTTDG KKMVDVLVEQ NIVPGIKVDK GLVPLVGSYD ESWCQGLDGL ASRTAAYYQQ 

       190        200        210        220        230        240 
GARFAKWRTV VSIPNGPSAL AVKEAAWGLA RYAAISQDSG LVPIVEPEIM LDGEHGIDRT 

       250        260        270        280        290        300 
YDVAEKVWAE VFFYLAQNNV MFEGILLKPS MVTPGAEATD RATPEQVASY TLKLLRNRIP 

       310        320        330        340        350        360 
PAVPGIMFLS GGQSELEATL NLNAMNQAPN PWHVSFSYAR ALQNTCLKTW GGKEENVKAA 

       370        380        390 
QDILLARAKA NSLAQLGKYT GEGESEEAKE GMFVKGYTY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles." Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C. J. Biol. Chem. 281:11225-11234(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: cv. Col-2.
[5]	"Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes." Ytterberg A.J., Peltier J.-B., van Wijk K.J. Plant Physiol. 140:984-997(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: cv. Columbia.
[6]	"Characterization of chloroplastic fructose 1,6-bisphosphate aldolases as lysine-methylated proteins in plants." Mininno M., Brugiere S., Pautre V., Gilgen A., Ma S., Ferro M., Tardif M., Alban C., Ravanel S. J. Biol. Chem. 287:21034-21044(2012) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT LYS-395.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006841 Genomic DNA. Translation: AAD23681.2. CP002685 Genomic DNA. Translation: AEC07160.1. AF419589 mRNA. Translation: AAL31921.1. AF428408 mRNA. Translation: AAL16176.1. AY035043 mRNA. Translation: AAK59548.1. AY049282 mRNA. Translation: AAK83624.1. AY049286 mRNA. Translation: AAK83628.1. AY062582 mRNA. Translation: AAL32660.1. AY090291 mRNA. Translation: AAL90952.1. AY128380 mRNA. Translation: AAM91583.1. AY128784 mRNA. Translation: AAM91184.1. AY142633 mRNA. Translation: AAN13091.1. BT000106 mRNA. Translation: AAN15425.1. BT002415 mRNA. Translation: AAO00775.1.
IPI	IPI00549111.
PIR	A84600.
RefSeq	NP_565508.1. NM_127705.3.
UniGene	At.21716. At.49175.
3D structure databases
ProteinModelPortal	Q9SJU4.
SMR	Q9SJU4. Positions 53-381.
ModBase	Search...
2D gel databases
World-2DPAGE	0003:Q9SJU4.
Proteomic databases
PaxDb	Q9SJU4.
PRIDE	Q9SJU4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G21330.1; AT2G21330.1; AT2G21330.
GeneID	816672.
KEGG	ath:AT2G21330.
Organism-specific databases
TAIR	At2g21330.
Phylogenomic databases
eggNOG	COG3588.
HOGENOM	HOG000220876.
InParanoid	Q9SJU4.
KO	K01623.
OMA	AEAKEGM.
PhylomeDB	Q9SJU4.
ProtClustDB	PLN02227.
Enzyme and pathway databases
UniPathway	UPA00109; UER00183.
Gene expression databases
ArrayExpress	Q9SJU4.
Genevestigator	Q9SJU4.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
PANTHER	PTHR11627. PTHR11627. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALFC1_ARATH

Accession

Primary (citable) accession number: Q9SJU4
Secondary accession number(s): Q2V473, Q93WF5, Q94C97

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents