Reviewed, UniProtKB/Swiss-Prot Q9SJU4 (ALFC1_ARATH)

Names and origin

Protein names

Recommended name:
    Probable fructose-bisphosphate aldolase 1, chloroplastic
    EC=4.1.2.13

Gene names

Name:	FBA1
Ordered Locus Names:	At2g21330
ORF Names:	F3K23.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subcellular location	Plastid › chloroplast › plastoglobule Ref.3 Ref.4.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast envelope Inferred from direct assay. Source: TAIR chloroplast stroma Ref.3 Inferred from direct assay. Source: TAIR cytosolic ribosome Inferred from direct assay. Source: TAIR plastoglobule Ref.3 Ref.4 Inferred from direct assay. Source: TAIR thylakoid lumen Inferred from direct assay. Source: TAIR
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SJU4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 10	10	Chloroplast Potential
	Chain	11 – 399	389	Probable fructose-bisphosphate aldolase 1, chloroplastic		PRO_0000286526
Sites
	Active site	226	1	Proton acceptor By similarity
	Active site	268	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	186	1	Substrate By similarity
	Site	399	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity
Experimental info
	Sequence conflict	236	1	G → S in AAK59548. Ref.2
	Sequence conflict	315	1	E → G in AAK59548. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 26CC71FA3C571B53	FASTA	399	42,931
	10 20 30 40 50 60 MASSTATMLK ASPVKSDWVK GQSLLLRQPS SVSAIRSHVA PSALTVRAAS AYADELVKTA 70 80 90 100 110 120 KTIASPGHGI MAMDESNATC GKRLASIGLE NTEANRQAYR TLLVSAPGLG QYISGAILFE 130 140 150 160 170 180 ETLYQSTTDG KKMVDVLVEQ NIVPGIKVDK GLVPLVGSYD ESWCQGLDGL ASRTAAYYQQ 190 200 210 220 230 240 GARFAKWRTV VSIPNGPSAL AVKEAAWGLA RYAAISQDSG LVPIVEPEIM LDGEHGIDRT 250 260 270 280 290 300 YDVAEKVWAE VFFYLAQNNV MFEGILLKPS MVTPGAEATD RATPEQVASY TLKLLRNRIP 310 320 330 340 350 360 PAVPGIMFLS GGQSELEATL NLNAMNQAPN PWHVSFSYAR ALQNTCLKTW GGKEENVKAA 370 380 390 QDILLARAKA NSLAQLGKYT GEGESEEAKE GMFVKGYTY

References

[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles." Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C. J. Biol. Chem. 281:11225-11234(2006) [PubMed: 16414959] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes." Ytterberg A.J., Peltier J.-B., van Wijk K.J. Plant Physiol. 140:984-997(2006) [PubMed: 16461379] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006841 Genomic DNA. Translation: AAD23681.2. AF419589 mRNA. Translation: AAL31921.1. AF428408 mRNA. Translation: AAL16176.1. AY035043 mRNA. Translation: AAK59548.1. AY049282 mRNA. Translation: AAK83624.1. AY049286 mRNA. Translation: AAK83628.1. AY062582 mRNA. Translation: AAL32660.1. AY090291 mRNA. Translation: AAL90952.1. AY128380 mRNA. Translation: AAM91583.1. AY128784 mRNA. Translation: AAM91184.1. AY142633 mRNA. Translation: AAN13091.1. BT000106 mRNA. Translation: AAN15425.1. BT002415 mRNA. Translation: AAO00775.1.
IPI	IPI00549111.
PIR	A84600.
RefSeq	NP_565508.1.
UniGene	At.21716 Rra.14531
3D structure databases
HSSP	HSSP built from PDB template 1XDL based on UniProtKB P05062.
SMR	Q9SJU4. Positions 50-383.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SJU4.
Proteomic databases
PRIDE	Q9SJU4.
ProMEX	Q9SJU4.
Genome annotation databases
GeneID	816672.
GenomeReviews	Gene locus AT2G21330 in contig CT485783_GR.
NMPDR	fig|3702.1.peg.9156.
Organism-specific databases
TAIR	At2g21330.
Phylogenomic databases
eggNOG	KOG1557.
HOGENOM	HBG559178.
InParanoid	Q9SJU4.
OMA	SVPGIMF.
PhylomeDB	Q9SJU4.
Enzyme and pathway databases
BRENDA	4.1.2.13. 302.
Gene expression databases
Genevestigator	Q9SJU4.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALFC1_ARATH

Accession

Primary (citable) accession number: Q9SJU4
Secondary accession number(s): Q2V473, Q93WF5, Q94C97

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)