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Q9SJU4 (ALFC1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable fructose-bisphosphate aldolase 1, chloroplastic

EC=4.1.2.13
Gene names
Name:FBA1
Ordered Locus Names:At2g21330
ORF Names:F3K23.9
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subcellular location

Plastidchloroplastplastoglobule Ref.4 Ref.5.

Post-translational modification

Can be trimethylated at Lys-395 by LSMT-L, but the trimethylation has no effect in vitro on the kinetic properties of the enzyme. Ref.6

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SJU4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1010Chloroplast Potential
Chain11 – 399389Probable fructose-bisphosphate aldolase 1, chloroplastic
PRO_0000286526

Sites

Active site2261Proton acceptor By similarity
Active site2681Schiff-base intermediate with dihydroxyacetone-P By similarity
Binding site961Substrate By similarity
Binding site1861Substrate By similarity
Site3991Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Amino acid modifications

Modified residue2161Phosphoserine By similarity
Modified residue3951N6,N6,N6-trimethyllysine Ref.6

Experimental info

Sequence conflict2361G → S in AAK59548. Ref.3
Sequence conflict3151E → G in AAK59548. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 26CC71FA3C571B53

FASTA39942,931
        10         20         30         40         50         60 
MASSTATMLK ASPVKSDWVK GQSLLLRQPS SVSAIRSHVA PSALTVRAAS AYADELVKTA 

        70         80         90        100        110        120 
KTIASPGHGI MAMDESNATC GKRLASIGLE NTEANRQAYR TLLVSAPGLG QYISGAILFE 

       130        140        150        160        170        180 
ETLYQSTTDG KKMVDVLVEQ NIVPGIKVDK GLVPLVGSYD ESWCQGLDGL ASRTAAYYQQ 

       190        200        210        220        230        240 
GARFAKWRTV VSIPNGPSAL AVKEAAWGLA RYAAISQDSG LVPIVEPEIM LDGEHGIDRT 

       250        260        270        280        290        300 
YDVAEKVWAE VFFYLAQNNV MFEGILLKPS MVTPGAEATD RATPEQVASY TLKLLRNRIP 

       310        320        330        340        350        360 
PAVPGIMFLS GGQSELEATL NLNAMNQAPN PWHVSFSYAR ALQNTCLKTW GGKEENVKAA 

       370        380        390 
QDILLARAKA NSLAQLGKYT GEGESEEAKE GMFVKGYTY 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles."
Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C.
J. Biol. Chem. 281:11225-11234(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Col-2.
[5]"Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes."
Ytterberg A.J., Peltier J.-B., van Wijk K.J.
Plant Physiol. 140:984-997(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
[6]"Characterization of chloroplastic fructose 1,6-bisphosphate aldolases as lysine-methylated proteins in plants."
Mininno M., Brugiere S., Pautre V., Gilgen A., Ma S., Ferro M., Tardif M., Alban C., Ravanel S.
J. Biol. Chem. 287:21034-21044(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT LYS-395.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006841 Genomic DNA. Translation: AAD23681.2.
CP002685 Genomic DNA. Translation: AEC07160.1.
AF419589 mRNA. Translation: AAL31921.1.
AF428408 mRNA. Translation: AAL16176.1.
AY035043 mRNA. Translation: AAK59548.1.
AY049282 mRNA. Translation: AAK83624.1.
AY049286 mRNA. Translation: AAK83628.1.
AY062582 mRNA. Translation: AAL32660.1.
AY090291 mRNA. Translation: AAL90952.1.
AY128380 mRNA. Translation: AAM91583.1.
AY128784 mRNA. Translation: AAM91184.1.
AY142633 mRNA. Translation: AAN13091.1.
BT000106 mRNA. Translation: AAN15425.1.
BT002415 mRNA. Translation: AAO00775.1.
PIRA84600.
RefSeqNP_565508.1. NM_127705.3.
UniGeneAt.21716.
At.49175.

3D structure databases

ProteinModelPortalQ9SJU4.
SMRQ9SJU4. Positions 53-381.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid2025. 1 interaction.

2D gel databases

World-2DPAGE0003:Q9SJU4.

Proteomic databases

PaxDbQ9SJU4.
PRIDEQ9SJU4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G21330.1; AT2G21330.1; AT2G21330. [Q9SJU4-1]
GeneID816672.
KEGGath:AT2G21330.

Organism-specific databases

TAIRAT2G21330.

Phylogenomic databases

eggNOGCOG3588.
HOGENOMHOG000220876.
InParanoidQ9SJU4.
KOK01623.
OMAWAETFKY.
PhylomeDBQ9SJU4.
ProtClustDBPLN02227.

Enzyme and pathway databases

BioCycARA:AT2G21330-MONOMER.
ARA:GQT-2337-MONOMER.
ARA:GQT-2338-MONOMER.
UniPathwayUPA00109; UER00183.

Gene expression databases

ArrayExpressQ9SJU4.
GenevestigatorQ9SJU4.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERPTHR11627. PTHR11627. 1 hit.
PfamPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALFC1_ARATH
AccessionPrimary (citable) accession number: Q9SJU4
Secondary accession number(s): Q2V473, Q93WF5, Q94C97
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names