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Protein

Probable fructose-bisphosphate aldolase 1, chloroplastic

Gene

FBA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway:iglycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC), Glucose-6-phosphate isomerase, cytosolic (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase 3 (PFK3), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), ATP-dependent 6-phosphofructokinase 2 (PFK2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase 7 (PFK7), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2)
  4. Fructose-bisphosphate aldolase (M3E9.40), Fructose-bisphosphate aldolase (F17C15_110), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA2), Probable fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (F8J2_100), Fructose-bisphosphate aldolase (F17C15.11), Fructose-bisphosphate aldolase, cytoplasmic isozyme (At4g26520), Probable fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (At3g52930), Probable fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At5g03690), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (At5g03690)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961SubstrateBy similarity
Binding sitei186 – 1861SubstrateBy similarity
Active sitei226 – 2261Proton acceptorBy similarity
Active sitei268 – 2681Schiff-base intermediate with dihydroxyacetone-PBy similarity
Sitei399 – 3991Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

  • glycolytic process Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciARA:AT2G21330-MONOMER.
ARA:GQT-2337-MONOMER.
ARA:GQT-2338-MONOMER.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable fructose-bisphosphate aldolase 1, chloroplastic (EC:4.1.2.13)
Gene namesi
Name:FBA1
Ordered Locus Names:At2g21330
ORF Names:F3K23.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G21330.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • cytosolic ribosome Source: TAIR
  • plastoglobule Source: TAIR
  • thylakoid Source: TAIR
  • thylakoid lumen Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848Chloroplast1 PublicationAdd
BLAST
Chaini49 – 399351Probable fructose-bisphosphate aldolase 1, chloroplasticPRO_0000286526Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161PhosphoserineBy similarity
Modified residuei395 – 3951N6,N6,N6-trimethyllysine1 Publication

Post-translational modificationi

Can be trimethylated at Lys-395 by LSMT-L, but the trimethylation has no effect in vitro on the kinetic properties of the enzyme.

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9SJU4.
PRIDEiQ9SJU4.

2D gel databases

World-2DPAGE0003:Q9SJU4.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SJU4. baseline and differential.

Interactioni

Protein-protein interaction databases

BioGridi2025. 1 interaction.
STRINGi3702.AT2G21330.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SJU4.
SMRiQ9SJU4. Positions 53-381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3588.
HOGENOMiHOG000220876.
InParanoidiQ9SJU4.
OMAiHGIDRTY.
PhylomeDBiQ9SJU4.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SJU4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSTATMLK ASPVKSDWVK GQSLLLRQPS SVSAIRSHVA PSALTVRAAS
60 70 80 90 100
AYADELVKTA KTIASPGHGI MAMDESNATC GKRLASIGLE NTEANRQAYR
110 120 130 140 150
TLLVSAPGLG QYISGAILFE ETLYQSTTDG KKMVDVLVEQ NIVPGIKVDK
160 170 180 190 200
GLVPLVGSYD ESWCQGLDGL ASRTAAYYQQ GARFAKWRTV VSIPNGPSAL
210 220 230 240 250
AVKEAAWGLA RYAAISQDSG LVPIVEPEIM LDGEHGIDRT YDVAEKVWAE
260 270 280 290 300
VFFYLAQNNV MFEGILLKPS MVTPGAEATD RATPEQVASY TLKLLRNRIP
310 320 330 340 350
PAVPGIMFLS GGQSELEATL NLNAMNQAPN PWHVSFSYAR ALQNTCLKTW
360 370 380 390
GGKEENVKAA QDILLARAKA NSLAQLGKYT GEGESEEAKE GMFVKGYTY
Length:399
Mass (Da):42,931
Last modified:June 1, 2002 - v2
Checksum:i26CC71FA3C571B53
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti236 – 2361G → S in AAK59548 (PubMed:14593172).Curated
Sequence conflicti315 – 3151E → G in AAK59548 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006841 Genomic DNA. Translation: AAD23681.2.
CP002685 Genomic DNA. Translation: AEC07160.1.
AF419589 mRNA. Translation: AAL31921.1.
AF428408 mRNA. Translation: AAL16176.1.
AY035043 mRNA. Translation: AAK59548.1.
AY049282 mRNA. Translation: AAK83624.1.
AY049286 mRNA. Translation: AAK83628.1.
AY062582 mRNA. Translation: AAL32660.1.
AY090291 mRNA. Translation: AAL90952.1.
AY128380 mRNA. Translation: AAM91583.1.
AY128784 mRNA. Translation: AAM91184.1.
AY142633 mRNA. Translation: AAN13091.1.
BT000106 mRNA. Translation: AAN15425.1.
BT002415 mRNA. Translation: AAO00775.1.
PIRiA84600.
RefSeqiNP_565508.1. NM_127705.3. [Q9SJU4-1]
UniGeneiAt.21716.
At.49175.

Genome annotation databases

EnsemblPlantsiAT2G21330.1; AT2G21330.1; AT2G21330. [Q9SJU4-1]
GeneIDi816672.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006841 Genomic DNA. Translation: AAD23681.2.
CP002685 Genomic DNA. Translation: AEC07160.1.
AF419589 mRNA. Translation: AAL31921.1.
AF428408 mRNA. Translation: AAL16176.1.
AY035043 mRNA. Translation: AAK59548.1.
AY049282 mRNA. Translation: AAK83624.1.
AY049286 mRNA. Translation: AAK83628.1.
AY062582 mRNA. Translation: AAL32660.1.
AY090291 mRNA. Translation: AAL90952.1.
AY128380 mRNA. Translation: AAM91583.1.
AY128784 mRNA. Translation: AAM91184.1.
AY142633 mRNA. Translation: AAN13091.1.
BT000106 mRNA. Translation: AAN15425.1.
BT002415 mRNA. Translation: AAO00775.1.
PIRiA84600.
RefSeqiNP_565508.1. NM_127705.3. [Q9SJU4-1]
UniGeneiAt.21716.
At.49175.

3D structure databases

ProteinModelPortaliQ9SJU4.
SMRiQ9SJU4. Positions 53-381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2025. 1 interaction.
STRINGi3702.AT2G21330.1.

2D gel databases

World-2DPAGE0003:Q9SJU4.

Proteomic databases

PaxDbiQ9SJU4.
PRIDEiQ9SJU4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G21330.1; AT2G21330.1; AT2G21330. [Q9SJU4-1]
GeneIDi816672.

Organism-specific databases

TAIRiAT2G21330.

Phylogenomic databases

eggNOGiCOG3588.
HOGENOMiHOG000220876.
InParanoidiQ9SJU4.
OMAiHGIDRTY.
PhylomeDBiQ9SJU4.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciARA:AT2G21330-MONOMER.
ARA:GQT-2337-MONOMER.
ARA:GQT-2338-MONOMER.

Miscellaneous databases

PROiQ9SJU4.

Gene expression databases

ExpressionAtlasiQ9SJU4. baseline and differential.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. Cited for: PROTEIN SEQUENCE OF 49-60, SUBCELLULAR LOCATION.
  5. "Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles."
    Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C.
    J. Biol. Chem. 281:11225-11234(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Col-2.
  6. "Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes."
    Ytterberg A.J., Peltier J.-B., van Wijk K.J.
    Plant Physiol. 140:984-997(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  7. "Characterization of chloroplastic fructose 1,6-bisphosphate aldolases as lysine-methylated proteins in plants."
    Mininno M., Brugiere S., Pautre V., Gilgen A., Ma S., Ferro M., Tardif M., Alban C., Ravanel S.
    J. Biol. Chem. 287:21034-21044(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT LYS-395.

Entry informationi

Entry nameiALFC1_ARATH
AccessioniPrimary (citable) accession number: Q9SJU4
Secondary accession number(s): Q2V473, Q93WF5, Q94C97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2002
Last modified: July 22, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.