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Reviewed, UniProtKB/Swiss-Prot Q9SJU4 (ALFC1_ARATH)

Last modified February 9, 2010. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Probable fructose-bisphosphate aldolase 1, chloroplastic
    EC=4.1.2.13
Gene names
Name: FBA1
Ordered Locus Names: At2g21330
ORF Names: F3K23.9
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subcellular location

Plastidchloroplastplastoglobule Ref.3 Ref.4.

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

response to cadmium ion

Inferred from expression pattern. Source: TAIR

   Cellular componentapoplast

Inferred from direct assay. Source: TAIR

chloroplast envelope

Inferred from direct assay. Source: TAIR

chloroplast stroma Ref.3

Inferred from direct assay. Source: TAIR

cytosolic ribosome

Inferred from direct assay. Source: TAIR

plastoglobule Ref.3 Ref.4

Inferred from direct assay. Source: TAIR

thylakoid lumen

Inferred from direct assay. Source: TAIR

   Molecular functionfructose-bisphosphate aldolase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SJU4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1010Chloroplast Potential
Chain11 – 399389Probable fructose-bisphosphate aldolase 1, chloroplastic
PRO_0000286526

Sites

Active site2261Proton acceptor By similarity
Active site2681Schiff-base intermediate with dihydroxyacetone-P By similarity
Binding site961Substrate By similarity
Binding site1861Substrate By similarity
Site3991Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Experimental info

Sequence conflict2361G → S in AAK59548. Ref.2
Sequence conflict3151E → G in AAK59548. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 26CC71FA3C571B53

FASTA39942,931
        10         20         30         40         50         60 
MASSTATMLK ASPVKSDWVK GQSLLLRQPS SVSAIRSHVA PSALTVRAAS AYADELVKTA 

        70         80         90        100        110        120 
KTIASPGHGI MAMDESNATC GKRLASIGLE NTEANRQAYR TLLVSAPGLG QYISGAILFE 

       130        140        150        160        170        180 
ETLYQSTTDG KKMVDVLVEQ NIVPGIKVDK GLVPLVGSYD ESWCQGLDGL ASRTAAYYQQ 

       190        200        210        220        230        240 
GARFAKWRTV VSIPNGPSAL AVKEAAWGLA RYAAISQDSG LVPIVEPEIM LDGEHGIDRT 

       250        260        270        280        290        300 
YDVAEKVWAE VFFYLAQNNV MFEGILLKPS MVTPGAEATD RATPEQVASY TLKLLRNRIP 

       310        320        330        340        350        360 
PAVPGIMFLS GGQSELEATL NLNAMNQAPN PWHVSFSYAR ALQNTCLKTW GGKEENVKAA 

       370        380        390 
QDILLARAKA NSLAQLGKYT GEGESEEAKE GMFVKGYTY 

« Hide

References

[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[3]"Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles."
Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C.
J. Biol. Chem. 281:11225-11234(2006) [PubMed: 16414959] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]"Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes."
Ytterberg A.J., Peltier J.-B., van Wijk K.J.
Plant Physiol. 140:984-997(2006) [PubMed: 16461379] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006841 Genomic DNA. Translation: AAD23681.2.
AF419589 mRNA. Translation: AAL31921.1.
AF428408 mRNA. Translation: AAL16176.1.
AY035043 mRNA. Translation: AAK59548.1.
AY049282 mRNA. Translation: AAK83624.1.
AY049286 mRNA. Translation: AAK83628.1.
AY062582 mRNA. Translation: AAL32660.1.
AY090291 mRNA. Translation: AAL90952.1.
AY128380 mRNA. Translation: AAM91583.1.
AY128784 mRNA. Translation: AAM91184.1.
AY142633 mRNA. Translation: AAN13091.1.
BT000106 mRNA. Translation: AAN15425.1.
BT002415 mRNA. Translation: AAO00775.1.
IPIIPI00549111.
PIRA84600.
RefSeqNP_565508.1.
UniGeneAt.21716
Rra.14531

3D structure databases

HSSPHSSP built from PDB template 1XDL based on UniProtKB P05062.
SMRQ9SJU4. Positions 50-383.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9SJU4.

Proteomic databases

PRIDEQ9SJU4.
ProMEXQ9SJU4.

Genome annotation databases

GeneID816672.
GenomeReviewsGene locus AT2G21330 in contig CT485783_GR.
NMPDRfig|3702.1.peg.9156.

Organism-specific databases

TAIRAt2g21330.

Phylogenomic databases

eggNOGKOG1557.
HOGENOMHBG559178.
InParanoidQ9SJU4.
OMASVPGIMF.
PhylomeDBQ9SJU4.

Enzyme and pathway databases

BRENDA4.1.2.13. 302.

Gene expression databases

GenevestigatorQ9SJU4.

Family and domain databases

InterProIPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHERPTHR11627. Aldolase_I. 1 hit.
PfamPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALFC1_ARATH
AccessionPrimary (citable) accession number: Q9SJU4
Secondary accession number(s): Q2V473, Q93WF5, Q94C97
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2002
Last modified: February 9, 2010
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents