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Protein

Protein ABSCISIC ACID-INSENSITIVE 5

Gene

ABI5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence.9 Publications

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • negative regulation of seed germination Source: TAIR
  • positive regulation of transcription, DNA-templated Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to chitin Source: TAIR
  • response to gibberellin Source: TAIR
  • response to salt stress Source: TAIR
  • response to water deprivation Source: TAIR
  • seed development Source: TAIR
  • seed germination Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Abscisic acid signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ABSCISIC ACID-INSENSITIVE 5
Alternative name(s):
Dc3 promoter-binding factor 1
Short name:
AtDPBF1
Protein GROWTH-INSENSITIVITY TO ABA 1
bZIP transcription factor 39
Short name:
AtbZIP39
Gene namesi
Name:ABI5
Synonyms:BZIP39, DPBF1, GIA1, NEM1
Ordered Locus Names:At2g36270
ORF Names:F2H17.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G36270.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Exhibits abscisic acid (ABA) insensitivity.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi391K → R: Loss of sumoylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003696051 – 442Protein ABSCISIC ACID-INSENSITIVE 5Add BLAST442

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42PhosphoserineSequence analysis1
Modified residuei64PhosphoserineBy similarity1
Modified residuei145PhosphoserineBy similarity1
Modified residuei201PhosphothreonineSequence analysis1
Cross-linki391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Post-translational modificationi

Phosphorylated by SRK2D and SRK2I in vitro.4 Publications
Ubiquitinated. AFP1, KEG and RPN10 mediate its proteasome-dependent degradation. Its stability or degradation plays a central role in abscisic acid response. Sumoylated at Lys-391 by SIZ1. Sumoylation protects ABI5 from proteasome degradation, attenuating ABA signaling and sensitivity to ABA.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9SJN0.

PTM databases

iPTMnetiQ9SJN0.

Expressioni

Tissue specificityi

Predominantly expressed in seeds.2 Publications

Developmental stagei

Expressed in embryo during the latest stages of seed maturation.1 Publication

Inductioni

Up-regulated by drought, salt, abscisic acid (ABA) and glucose or 2-deoxy-glucose (2DG). Autoregulated. Positively regulated by the light-signaling component HY5.6 Publications

Gene expression databases

GenevisibleiQ9SJN0. AT.

Interactioni

Subunit structurei

DNA-binding homodimer. DNA-binding heterodimer with AREB3/DPBF3 or EEL/DPBF4. Interacts with ABI3, KEG, the mediator subunit MED25, and the AFP proteins AFP1, AFP2, AFP3 and AFP4.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KEGQ9FY482EBI-1778690,EBI-1955729

Protein-protein interaction databases

BioGridi3543. 56 interactors.
DIPiDIP-40551N.
IntActiQ9SJN0. 7 interactors.
STRINGi3702.AT2G36270.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SJN0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini355 – 418bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni357 – 376Basic motifPROSITE-ProRule annotationAdd BLAST20
Regioni383 – 404Leucine-zipperPROSITE-ProRule annotationAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi76 – 109Asn-richAdd BLAST34
Compositional biasi250 – 342Gly-richAdd BLAST93

Sequence similaritiesi

Belongs to the bZIP family. ABI5 subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IHGD. Eukaryota.
ENOG4110YI4. LUCA.
HOGENOMiHOG000237278.
InParanoidiQ9SJN0.
KOiK14432.
OMAiHSVPANH.
OrthoDBiEOG09360F7U.
PhylomeDBiQ9SJN0.

Family and domain databases

InterProiIPR029803. ABI5.
IPR004827. bZIP.
[Graphical view]
PANTHERiPTHR22952:SF217. PTHR22952:SF217. 3 hits.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SJN0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTRETKLTS EREVESSMAQ ARHNGGGGGE NHPFTSLGRQ SSIYSLTLDE
60 70 80 90 100
FQHALCENGK NFGSMNMDEF LVSIWNAEEN NNNQQQAAAA AGSHSVPANH
110 120 130 140 150
NGFNNNNNNG GEGGVGVFSG GSRGNEDANN KRGIANESSL PRQGSLTLPA
160 170 180 190 200
PLCRKTVDEV WSEIHRGGGS GNGGDSNGRS SSSNGQNNAQ NGGETAARQP
210 220 230 240 250
TFGEMTLEDF LVKAGVVREH PTNPKPNPNP NQNQNPSSVI PAAAQQQLYG
260 270 280 290 300
VFQGTGDPSF PGQAMGVGDP SGYAKRTGGG GYQQAPPVQA GVCYGGGVGF
310 320 330 340 350
GAGGQQMGMV GPLSPVSSDG LGHGQVDNIG GQYGVDMGGL RGRKRVVDGP
360 370 380 390 400
VEKVVERRQR RMIKNRESAA RSRARKQAYT VELEAELNQL KEENAQLKHA
410 420 430 440
LAELERKRKQ QYFESLKSRA QPKLPKSNGR LRTLMRNPSC PL
Length:442
Mass (Da):47,007
Last modified:May 1, 2000 - v1
Checksum:iF879F51B99A99140
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334206 mRNA. Translation: AAK19599.1.
AC006921 Genomic DNA. Translation: AAD21438.1.
CP002685 Genomic DNA. Translation: AEC09226.1.
BT026517 mRNA. Translation: ABH04624.1.
PIRiG84778.
RefSeqiNP_565840.1. NM_129185.4.
UniGeneiAt.14114.

Genome annotation databases

EnsemblPlantsiAT2G36270.1; AT2G36270.1; AT2G36270.
GeneIDi818199.
GrameneiAT2G36270.1; AT2G36270.1; AT2G36270.
KEGGiath:AT2G36270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334206 mRNA. Translation: AAK19599.1.
AC006921 Genomic DNA. Translation: AAD21438.1.
CP002685 Genomic DNA. Translation: AEC09226.1.
BT026517 mRNA. Translation: ABH04624.1.
PIRiG84778.
RefSeqiNP_565840.1. NM_129185.4.
UniGeneiAt.14114.

3D structure databases

ProteinModelPortaliQ9SJN0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3543. 56 interactors.
DIPiDIP-40551N.
IntActiQ9SJN0. 7 interactors.
STRINGi3702.AT2G36270.1.

PTM databases

iPTMnetiQ9SJN0.

Proteomic databases

PaxDbiQ9SJN0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G36270.1; AT2G36270.1; AT2G36270.
GeneIDi818199.
GrameneiAT2G36270.1; AT2G36270.1; AT2G36270.
KEGGiath:AT2G36270.

Organism-specific databases

TAIRiAT2G36270.

Phylogenomic databases

eggNOGiENOG410IHGD. Eukaryota.
ENOG4110YI4. LUCA.
HOGENOMiHOG000237278.
InParanoidiQ9SJN0.
KOiK14432.
OMAiHSVPANH.
OrthoDBiEOG09360F7U.
PhylomeDBiQ9SJN0.

Miscellaneous databases

PROiQ9SJN0.

Gene expression databases

GenevisibleiQ9SJN0. AT.

Family and domain databases

InterProiIPR029803. ABI5.
IPR004827. bZIP.
[Graphical view]
PANTHERiPTHR22952:SF217. PTHR22952:SF217. 3 hits.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiABI5_ARATH
AccessioniPrimary (citable) accession number: Q9SJN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.