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Q9SJN0

- ABI5_ARATH

UniProt

Q9SJN0 - ABI5_ARATH

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Protein

Protein ABSCISIC ACID-INSENSITIVE 5

Gene

ABI5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence.9 Publications

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: UniProtKB-KW
  2. negative regulation of seed germination Source: TAIR
  3. positive regulation of transcription, DNA-templated Source: TAIR
  4. regulation of transcription, DNA-templated Source: TAIR
  5. response to abscisic acid Source: TAIR
  6. response to chitin Source: TAIR
  7. response to gibberellin Source: TAIR
  8. response to salt stress Source: TAIR
  9. response to water deprivation Source: TAIR
  10. seed development Source: TAIR
  11. seed germination Source: TAIR
  12. sugar mediated signaling pathway Source: TAIR
  13. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Abscisic acid signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ABSCISIC ACID-INSENSITIVE 5
Alternative name(s):
Dc3 promoter-binding factor 1
Short name:
AtDPBF1
Protein GROWTH-INSENSITIVITY TO ABA 1
bZIP transcription factor 39
Short name:
AtbZIP39
Gene namesi
Name:ABI5
Synonyms:BZIP39, DPBF1, GIA1, NEM1
Ordered Locus Names:At2g36270
ORF Names:F2H17.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G36270.

Subcellular locationi

Nucleus 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Exhibits abscisic acid (ABA) insensitivity.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi391 – 3911K → R: Loss of sumoylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442Protein ABSCISIC ACID-INSENSITIVE 5PRO_0000369605Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421PhosphoserineSequence Analysis
Modified residuei64 – 641PhosphoserineBy similarity
Modified residuei145 – 1451PhosphoserineBy similarity
Modified residuei201 – 2011PhosphothreonineSequence Analysis
Cross-linki391 – 391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modificationi

Phosphorylated by SRK2D and SRK2I in vitro.4 Publications
Ubiquitinated. AFP1, KEG and RPN10 mediate its proteasome-dependent degradation. Its stability or degradation plays a central role in abscisic acid response. Sumoylated at Lys-391 by SIZ1. Sumoylation protects ABI5 from proteasome degradation, attenuating ABA signaling and sensitivity to ABA.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ9SJN0.

Expressioni

Tissue specificityi

Predominantly expressed in seeds.2 Publications

Developmental stagei

Expressed in embryo during the latest stages of seed maturation.1 Publication

Inductioni

Up-regulated by drought, salt, abscisic acid (ABA) and glucose or 2-deoxy-glucose (2DG). Autoregulated. Positively regulated by the light-signaling component HY5.6 Publications

Gene expression databases

GenevestigatoriQ9SJN0.

Interactioni

Subunit structurei

DNA-binding homodimer. DNA-binding heterodimer with AREB3/DPBF3 or EEL/DPBF4. Interacts with ABI3, KEG, the mediator subunit MED25, and the AFP proteins AFP1, AFP2, AFP3 and AFP4.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KEGQ9FY482EBI-1778690,EBI-1955729

Protein-protein interaction databases

BioGridi3543. 47 interactions.
DIPiDIP-40551N.
IntActiQ9SJN0. 7 interactions.
STRINGi3702.AT2G36270.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SJN0.
SMRiQ9SJN0. Positions 350-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini355 – 41864bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni357 – 37620Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni383 – 40422Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi76 – 10934Asn-richAdd
BLAST
Compositional biasi250 – 34293Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the bZIP family. ABI5 subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG251869.
HOGENOMiHOG000237278.
InParanoidiQ9SJN0.
KOiK14432.
OMAiCENGKNF.
PhylomeDBiQ9SJN0.

Family and domain databases

InterProiIPR004827. bZIP.
[Graphical view]
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SJN0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTRETKLTS EREVESSMAQ ARHNGGGGGE NHPFTSLGRQ SSIYSLTLDE
60 70 80 90 100
FQHALCENGK NFGSMNMDEF LVSIWNAEEN NNNQQQAAAA AGSHSVPANH
110 120 130 140 150
NGFNNNNNNG GEGGVGVFSG GSRGNEDANN KRGIANESSL PRQGSLTLPA
160 170 180 190 200
PLCRKTVDEV WSEIHRGGGS GNGGDSNGRS SSSNGQNNAQ NGGETAARQP
210 220 230 240 250
TFGEMTLEDF LVKAGVVREH PTNPKPNPNP NQNQNPSSVI PAAAQQQLYG
260 270 280 290 300
VFQGTGDPSF PGQAMGVGDP SGYAKRTGGG GYQQAPPVQA GVCYGGGVGF
310 320 330 340 350
GAGGQQMGMV GPLSPVSSDG LGHGQVDNIG GQYGVDMGGL RGRKRVVDGP
360 370 380 390 400
VEKVVERRQR RMIKNRESAA RSRARKQAYT VELEAELNQL KEENAQLKHA
410 420 430 440
LAELERKRKQ QYFESLKSRA QPKLPKSNGR LRTLMRNPSC PL
Length:442
Mass (Da):47,007
Last modified:May 1, 2000 - v1
Checksum:iF879F51B99A99140
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF334206 mRNA. Translation: AAK19599.1.
AC006921 Genomic DNA. Translation: AAD21438.1.
CP002685 Genomic DNA. Translation: AEC09226.1.
BT026517 mRNA. Translation: ABH04624.1.
PIRiG84778.
RefSeqiNP_565840.1. NM_129185.3.
UniGeneiAt.14114.

Genome annotation databases

EnsemblPlantsiAT2G36270.1; AT2G36270.1; AT2G36270.
GeneIDi818199.
KEGGiath:AT2G36270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF334206 mRNA. Translation: AAK19599.1 .
AC006921 Genomic DNA. Translation: AAD21438.1 .
CP002685 Genomic DNA. Translation: AEC09226.1 .
BT026517 mRNA. Translation: ABH04624.1 .
PIRi G84778.
RefSeqi NP_565840.1. NM_129185.3.
UniGenei At.14114.

3D structure databases

ProteinModelPortali Q9SJN0.
SMRi Q9SJN0. Positions 350-406.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 3543. 47 interactions.
DIPi DIP-40551N.
IntActi Q9SJN0. 7 interactions.
STRINGi 3702.AT2G36270.1-P.

Proteomic databases

PRIDEi Q9SJN0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G36270.1 ; AT2G36270.1 ; AT2G36270 .
GeneIDi 818199.
KEGGi ath:AT2G36270.

Organism-specific databases

TAIRi AT2G36270.

Phylogenomic databases

eggNOGi NOG251869.
HOGENOMi HOG000237278.
InParanoidi Q9SJN0.
KOi K14432.
OMAi CENGKNF.
PhylomeDBi Q9SJN0.

Gene expression databases

Genevestigatori Q9SJN0.

Family and domain databases

InterProi IPR004827. bZIP.
[Graphical view ]
Pfami PF00170. bZIP_1. 1 hit.
[Graphical view ]
SMARTi SM00338. BRLZ. 1 hit.
[Graphical view ]
PROSITEi PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor."
    Finkelstein R.R., Lynch T.J.
    Plant Cell 12:599-609(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
  2. "Arabidopsis ABI5 subfamily members have distinct DNA-binding and transcriptional activities."
    Kim S.Y., Ma J., Perret P., Li Z., Thomas T.L.
    Plant Physiol. 130:688-697(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY, DNA-BINDING, HETERODIMERIZATION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Arabidopsis ORF Clones."
    Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana."
    Lopez-Molina L., Chua N.H.
    Plant Cell Physiol. 41:541-547(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Physical interactions between ABA response loci of Arabidopsis."
    Nakamura S., Lynch T.J., Finkelstein R.R.
    Plant J. 26:627-635(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ABI3, HOMODIMERIZATION.
  8. "A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis."
    Lopez-Molina L., Mongrand S., Chua N.-H.
    Proc. Natl. Acad. Sci. U.S.A. 98:4782-4787(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, PHOSPHORYLATION.
  9. "The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis."
    Bensmihen S., Rippa S., Lambert G., Jublot D., Pautot V., Granier F., Giraudat J., Parcy F.
    Plant Cell 14:1391-1403(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  10. Cited for: FUNCTION, DNA-BINDING.
  11. "ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination."
    Lopez-Molina L., Mongrand S., McLachlin D.T., Chait B.T., Chua N.-H.
    Plant J. 32:317-328(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING, PHOSPHORYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
  12. "Regulation and role of the Arabidopsis abscisic acid-insensitive 5 gene in abscisic acid, sugar, and stress response."
    Brocard I.M., Lynch T.J., Finkelstein R.R.
    Plant Physiol. 129:1533-1543(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  13. "Mitogen-activated protein kinase signaling in postgermination arrest of development by abscisic acid."
    Lu C., Han M.-H., Guevara-Garcia A., Fedoroff N.V.
    Proc. Natl. Acad. Sci. U.S.A. 99:15812-15817(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  14. Cited for: GENE FAMILY, NOMENCLATURE.
  15. "AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation."
    Lopez-Molina L., Mongrand S., Kinoshita N., Chua N.-H.
    Genes Dev. 17:410-418(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN DEGRADATION, INTERACTION WITH AFP1.
  16. "The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling."
    Smalle J., Kurepa J., Yang P., Emborg T.J., Babiychuk E., Kushnir S., Vierstra R.D.
    Plant Cell 15:965-980(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN DEGRADATION.
  17. "Three genes that affect sugar sensing (abscisic acid insensitive 4, abscisic acid insensitive 5, and constitutive triple response 1) are differentially regulated by glucose in Arabidopsis."
    Arroyo A., Bossi F., Finkelstein R.R., Leon P.
    Plant Physiol. 133:231-242(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  18. "Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence."
    Pourtau N., Mares M., Purdy S., Quentin N., Rueel A., Wingler A.
    Planta 219:765-772(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  19. "Redundant and distinct functions of the ABA response loci ABA-INSENSITIVE(ABI)5 and ABRE-BINDING FACTOR (ABF)3."
    Finkelstein R.R., Gampala S.S., Lynch T.J., Thomas T.L., Rock C.D.
    Plant Mol. Biol. 59:253-267(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ABI3.
  20. "KEEP ON GOING, a RING E3 ligase essential for Arabidopsis growth and development, is involved in abscisic acid signaling."
    Stone S.L., Williams L.A., Farmer L.M., Vierstra R.D., Callis J.
    Plant Cell 18:3415-3428(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN DEGRADATION, INTERACTION WITH KEG.
  21. "Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis."
    Nakashima K., Fujita Y., Katsura K., Maruyama K., Narusaka Y., Seki M., Shinozaki K., Yamaguchi-Shinozaki K.
    Plant Mol. Biol. 60:51-68(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  22. "Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis."
    Fujii H., Verslues P.E., Zhu J.-K.
    Plant Cell 19:485-494(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY SRK2D AND SRK2I.
  23. "A small plant-specific protein family of ABI five binding proteins (AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings."
    Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R.
    Plant Mol. Biol. 67:643-658(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AFP1; AFP2; AFP3 AND AFP4.
  24. "Integration of light and abscisic acid signaling during seed germination and early seedling development."
    Chen H., Zhang J., Neff M.M., Hong S.-W., Zhang H., Deng X.-W., Xiong L.
    Proc. Natl. Acad. Sci. U.S.A. 105:4495-4500(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  25. "Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling."
    Miura K., Lee J., Jin J.B., Yoo C.Y., Miura T., Hasegawa P.M.
    Proc. Natl. Acad. Sci. U.S.A. 106:5418-5423(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION AT LYS-391, MUTAGENESIS OF LYS-391.
  26. "The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors."
    Chen R., Jiang H., Li L., Zhai Q., Qi L., Zhou W., Liu X., Li H., Zheng W., Sun J., Li C.
    Plant Cell 24:2898-2916(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED25.

Entry informationi

Entry nameiABI5_ARATH
AccessioniPrimary (citable) accession number: Q9SJN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3