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Q9SJN0 (ABI5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein ABSCISIC ACID-INSENSITIVE 5
Alternative name(s):
Dc3 promoter-binding factor 1
Short name=AtDPBF1
Protein GROWTH-INSENSITIVITY TO ABA 1
bZIP transcription factor 39
Short name=AtbZIP39
Gene names
Name:ABI5
Synonyms:BZIP39, DPBF1, GIA1, NEM1
Ordered Locus Names:At2g36270
ORF Names:F2H17.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.18 Ref.19 Ref.21

Subunit structure

DNA-binding homodimer. DNA-binding heterodimer with AREB3/DPBF3 or EEL/DPBF4. Interacts with ABI3, KEG, the mediator subunit MED25, and the AFP proteins AFP1, AFP2, AFP3 and AFP4. Ref.2 Ref.7 Ref.15 Ref.19 Ref.20 Ref.23 Ref.26

Subcellular location

Nucleus Ref.11.

Tissue specificity

Predominantly expressed in seeds. Ref.1 Ref.2

Developmental stage

Expressed in embryo during the latest stages of seed maturation. Ref.9

Induction

Up-regulated by drought, salt, abscisic acid (ABA) and glucose or 2-deoxy-glucose (2DG). Autoregulated. Positively regulated by the light-signaling component HY5. Ref.2 Ref.8 Ref.12 Ref.17 Ref.21 Ref.24

Post-translational modification

Phosphorylated by SRK2D and SRK2I in vitro. Ref.8 Ref.11 Ref.13 Ref.22

Ubiquitinated. AFP1, KEG and RPN10 mediate its proteasome-dependent degradation. Its stability or degradation plays a central role in abscisic acid response. Sumoylated at Lys-391 by SIZ1. Sumoylation protects ABI5 from proteasome degradation, attenuating ABA signaling and sensitivity to ABA. Ref.15 Ref.16 Ref.20

Disruption phenotype

Exhibits abscisic acid (ABA) insensitivity. Ref.6

Sequence similarities

Belongs to the bZIP family. ABI5 subfamily.

Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
   Biological processAbscisic acid signaling pathway
Transcription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
   PTMIsopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processabscisic acid-activated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of seed germination

Inferred from mutant phenotype PubMed 18941053. Source: TAIR

positive regulation of transcription, DNA-templated

Inferred from direct assay Ref.21PubMed 19207209. Source: TAIR

regulation of transcription, DNA-templated

Inferred from mutant phenotype Ref.1. Source: TAIR

response to abscisic acid

Inferred from mutant phenotype Ref.8. Source: TAIR

response to chitin

Inferred from expression pattern PubMed 17722694. Source: TAIR

response to gibberellin

Inferred from expression pattern PubMed 18941053. Source: TAIR

response to salt stress

Inferred from expression pattern Ref.8. Source: TAIR

response to water deprivation

Inferred from expression pattern Ref.8. Source: TAIR

seed development

Inferred from mutant phenotype Ref.1. Source: TAIR

seed germination

Inferred from expression pattern PubMed 18941053. Source: TAIR

sugar mediated signaling pathway

Traceable author statement PubMed 12663220. Source: TAIR

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 15642716. Source: TAIR

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 20525848. Source: UniProtKB

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from sequence or structural similarity PubMed 11118137Ref.14. Source: TAIR

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

KEGQ9FY482EBI-1778690,EBI-1955729

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 442442Protein ABSCISIC ACID-INSENSITIVE 5
PRO_0000369605

Regions

Domain355 – 41864bZIP
Region357 – 37620Basic motif By similarity
Region383 – 40422Leucine-zipper By similarity
Compositional bias76 – 10934Asn-rich
Compositional bias250 – 34293Gly-rich

Amino acid modifications

Modified residue421Phosphoserine Potential
Modified residue641Phosphoserine By similarity
Modified residue1451Phosphoserine By similarity
Modified residue2011Phosphothreonine Potential
Cross-link391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.25

Experimental info

Mutagenesis3911K → R: Loss of sumoylation. Ref.25

Sequences

Sequence LengthMass (Da)Tools
Q9SJN0 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: F879F51B99A99140

FASTA44247,007
        10         20         30         40         50         60 
MVTRETKLTS EREVESSMAQ ARHNGGGGGE NHPFTSLGRQ SSIYSLTLDE FQHALCENGK 

        70         80         90        100        110        120 
NFGSMNMDEF LVSIWNAEEN NNNQQQAAAA AGSHSVPANH NGFNNNNNNG GEGGVGVFSG 

       130        140        150        160        170        180 
GSRGNEDANN KRGIANESSL PRQGSLTLPA PLCRKTVDEV WSEIHRGGGS GNGGDSNGRS 

       190        200        210        220        230        240 
SSSNGQNNAQ NGGETAARQP TFGEMTLEDF LVKAGVVREH PTNPKPNPNP NQNQNPSSVI 

       250        260        270        280        290        300 
PAAAQQQLYG VFQGTGDPSF PGQAMGVGDP SGYAKRTGGG GYQQAPPVQA GVCYGGGVGF 

       310        320        330        340        350        360 
GAGGQQMGMV GPLSPVSSDG LGHGQVDNIG GQYGVDMGGL RGRKRVVDGP VEKVVERRQR 

       370        380        390        400        410        420 
RMIKNRESAA RSRARKQAYT VELEAELNQL KEENAQLKHA LAELERKRKQ QYFESLKSRA 

       430        440 
QPKLPKSNGR LRTLMRNPSC PL 

« Hide

References

« Hide 'large scale' references
[1]"The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor."
Finkelstein R.R., Lynch T.J.
Plant Cell 12:599-609(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[2]"Arabidopsis ABI5 subfamily members have distinct DNA-binding and transcriptional activities."
Kim S.Y., Ma J., Perret P., Li Z., Thomas T.L.
Plant Physiol. 130:688-697(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY, DNA-BINDING, HETERODIMERIZATION.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Arabidopsis ORF Clones."
Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana."
Lopez-Molina L., Chua N.H.
Plant Cell Physiol. 41:541-547(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[7]"Physical interactions between ABA response loci of Arabidopsis."
Nakamura S., Lynch T.J., Finkelstein R.R.
Plant J. 26:627-635(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ABI3, HOMODIMERIZATION.
[8]"A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis."
Lopez-Molina L., Mongrand S., Chua N.-H.
Proc. Natl. Acad. Sci. U.S.A. 98:4782-4787(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, PHOSPHORYLATION.
[9]"The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis."
Bensmihen S., Rippa S., Lambert G., Jublot D., Pautot V., Granier F., Giraudat J., Parcy F.
Plant Cell 14:1391-1403(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[10]"Regulation of Arabidopsis thaliana Em genes: role of ABI5."
Carles C., Bies-Etheve N., Aspart L., Leon-Kloosterziel K.M., Koornneef M., Echeverria M., Delseny M.
Plant J. 30:373-383(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING.
[11]"ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination."
Lopez-Molina L., Mongrand S., McLachlin D.T., Chait B.T., Chua N.-H.
Plant J. 32:317-328(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING, PHOSPHORYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
[12]"Regulation and role of the Arabidopsis abscisic acid-insensitive 5 gene in abscisic acid, sugar, and stress response."
Brocard I.M., Lynch T.J., Finkelstein R.R.
Plant Physiol. 129:1533-1543(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[13]"Mitogen-activated protein kinase signaling in postgermination arrest of development by abscisic acid."
Lu C., Han M.-H., Guevara-Garcia A., Fedoroff N.V.
Proc. Natl. Acad. Sci. U.S.A. 99:15812-15817(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION.
[14]"bZIP transcription factors in Arabidopsis."
Jakoby M., Weisshaar B., Droege-Laser W., Vicente-Carbajosa J., Tiedemann J., Kroj T., Parcy F.
Trends Plant Sci. 7:106-111(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[15]"AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation."
Lopez-Molina L., Mongrand S., Kinoshita N., Chua N.-H.
Genes Dev. 17:410-418(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN DEGRADATION, INTERACTION WITH AFP1.
[16]"The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling."
Smalle J., Kurepa J., Yang P., Emborg T.J., Babiychuk E., Kushnir S., Vierstra R.D.
Plant Cell 15:965-980(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN DEGRADATION.
[17]"Three genes that affect sugar sensing (abscisic acid insensitive 4, abscisic acid insensitive 5, and constitutive triple response 1) are differentially regulated by glucose in Arabidopsis."
Arroyo A., Bossi F., Finkelstein R.R., Leon P.
Plant Physiol. 133:231-242(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[18]"Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence."
Pourtau N., Mares M., Purdy S., Quentin N., Rueel A., Wingler A.
Planta 219:765-772(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[19]"Redundant and distinct functions of the ABA response loci ABA-INSENSITIVE(ABI)5 and ABRE-BINDING FACTOR (ABF)3."
Finkelstein R.R., Gampala S.S., Lynch T.J., Thomas T.L., Rock C.D.
Plant Mol. Biol. 59:253-267(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ABI3.
[20]"KEEP ON GOING, a RING E3 ligase essential for Arabidopsis growth and development, is involved in abscisic acid signaling."
Stone S.L., Williams L.A., Farmer L.M., Vierstra R.D., Callis J.
Plant Cell 18:3415-3428(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN DEGRADATION, INTERACTION WITH KEG.
[21]"Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis."
Nakashima K., Fujita Y., Katsura K., Maruyama K., Narusaka Y., Seki M., Shinozaki K., Yamaguchi-Shinozaki K.
Plant Mol. Biol. 60:51-68(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[22]"Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis."
Fujii H., Verslues P.E., Zhu J.-K.
Plant Cell 19:485-494(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY SRK2D AND SRK2I.
[23]"A small plant-specific protein family of ABI five binding proteins (AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings."
Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R.
Plant Mol. Biol. 67:643-658(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AFP1; AFP2; AFP3 AND AFP4.
[24]"Integration of light and abscisic acid signaling during seed germination and early seedling development."
Chen H., Zhang J., Neff M.M., Hong S.-W., Zhang H., Deng X.-W., Xiong L.
Proc. Natl. Acad. Sci. U.S.A. 105:4495-4500(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[25]"Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling."
Miura K., Lee J., Jin J.B., Yoo C.Y., Miura T., Hasegawa P.M.
Proc. Natl. Acad. Sci. U.S.A. 106:5418-5423(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUMOYLATION AT LYS-391, MUTAGENESIS OF LYS-391.
[26]"The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors."
Chen R., Jiang H., Li L., Zhai Q., Qi L., Zhou W., Liu X., Li H., Zheng W., Sun J., Li C.
Plant Cell 24:2898-2916(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MED25.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF334206 mRNA. Translation: AAK19599.1.
AC006921 Genomic DNA. Translation: AAD21438.1.
CP002685 Genomic DNA. Translation: AEC09226.1.
BT026517 mRNA. Translation: ABH04624.1.
PIRG84778.
RefSeqNP_565840.1. NM_129185.3.
UniGeneAt.14114.

3D structure databases

ProteinModelPortalQ9SJN0.
SMRQ9SJN0. Positions 360-405.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid3543. 20 interactions.
DIPDIP-40551N.
IntActQ9SJN0. 7 interactions.
STRING3702.AT2G36270.1-P.

Proteomic databases

PRIDEQ9SJN0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G36270.1; AT2G36270.1; AT2G36270.
GeneID818199.
KEGGath:AT2G36270.

Organism-specific databases

TAIRAT2G36270.

Phylogenomic databases

eggNOGNOG251869.
HOGENOMHOG000237278.
InParanoidQ9SJN0.
KOK14432.
OMACENGKNF.
PhylomeDBQ9SJN0.

Gene expression databases

GenevestigatorQ9SJN0.

Family and domain databases

InterProIPR004827. bZIP.
[Graphical view]
PfamPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameABI5_ARATH
AccessionPrimary (citable) accession number: Q9SJN0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names