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Q9SJN0

- ABI5_ARATH

UniProt

Q9SJN0 - ABI5_ARATH

Protein

Protein ABSCISIC ACID-INSENSITIVE 5

Gene

ABI5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence.9 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. sequence-specific DNA binding Source: InterPro
    3. sequence-specific DNA binding transcription factor activity Source: TAIR

    GO - Biological processi

    1. abscisic acid-activated signaling pathway Source: UniProtKB-KW
    2. negative regulation of seed germination Source: TAIR
    3. positive regulation of transcription, DNA-templated Source: TAIR
    4. regulation of transcription, DNA-templated Source: TAIR
    5. response to abscisic acid Source: TAIR
    6. response to chitin Source: TAIR
    7. response to gibberellin Source: TAIR
    8. response to salt stress Source: TAIR
    9. response to water deprivation Source: TAIR
    10. seed development Source: TAIR
    11. seed germination Source: TAIR
    12. sugar mediated signaling pathway Source: TAIR
    13. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Abscisic acid signaling pathway, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein ABSCISIC ACID-INSENSITIVE 5
    Alternative name(s):
    Dc3 promoter-binding factor 1
    Short name:
    AtDPBF1
    Protein GROWTH-INSENSITIVITY TO ABA 1
    bZIP transcription factor 39
    Short name:
    AtbZIP39
    Gene namesi
    Name:ABI5
    Synonyms:BZIP39, DPBF1, GIA1, NEM1
    Ordered Locus Names:At2g36270
    ORF Names:F2H17.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G36270.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Exhibits abscisic acid (ABA) insensitivity.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi391 – 3911K → R: Loss of sumoylation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 442442Protein ABSCISIC ACID-INSENSITIVE 5PRO_0000369605Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei42 – 421PhosphoserineSequence Analysis
    Modified residuei64 – 641PhosphoserineBy similarity
    Modified residuei145 – 1451PhosphoserineBy similarity
    Modified residuei201 – 2011PhosphothreonineSequence Analysis
    Cross-linki391 – 391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

    Post-translational modificationi

    Phosphorylated by SRK2D and SRK2I in vitro.4 Publications
    Ubiquitinated. AFP1, KEG and RPN10 mediate its proteasome-dependent degradation. Its stability or degradation plays a central role in abscisic acid response. Sumoylated at Lys-391 by SIZ1. Sumoylation protects ABI5 from proteasome degradation, attenuating ABA signaling and sensitivity to ABA.1 Publication

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ9SJN0.

    Expressioni

    Tissue specificityi

    Predominantly expressed in seeds.2 Publications

    Developmental stagei

    Expressed in embryo during the latest stages of seed maturation.1 Publication

    Inductioni

    Up-regulated by drought, salt, abscisic acid (ABA) and glucose or 2-deoxy-glucose (2DG). Autoregulated. Positively regulated by the light-signaling component HY5.6 Publications

    Gene expression databases

    GenevestigatoriQ9SJN0.

    Interactioni

    Subunit structurei

    DNA-binding homodimer. DNA-binding heterodimer with AREB3/DPBF3 or EEL/DPBF4. Interacts with ABI3, KEG, the mediator subunit MED25, and the AFP proteins AFP1, AFP2, AFP3 and AFP4.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    KEGQ9FY482EBI-1778690,EBI-1955729

    Protein-protein interaction databases

    BioGridi3543. 47 interactions.
    DIPiDIP-40551N.
    IntActiQ9SJN0. 7 interactions.
    STRINGi3702.AT2G36270.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SJN0.
    SMRiQ9SJN0. Positions 360-405.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini355 – 41864bZIPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni357 – 37620Basic motifPROSITE-ProRule annotationAdd
    BLAST
    Regioni383 – 40422Leucine-zipperPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi76 – 10934Asn-richAdd
    BLAST
    Compositional biasi250 – 34293Gly-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bZIP family. ABI5 subfamily.Curated
    Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG251869.
    HOGENOMiHOG000237278.
    InParanoidiQ9SJN0.
    KOiK14432.
    OMAiCENGKNF.
    PhylomeDBiQ9SJN0.

    Family and domain databases

    InterProiIPR004827. bZIP.
    [Graphical view]
    PfamiPF00170. bZIP_1. 1 hit.
    [Graphical view]
    SMARTiSM00338. BRLZ. 1 hit.
    [Graphical view]
    PROSITEiPS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SJN0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTRETKLTS EREVESSMAQ ARHNGGGGGE NHPFTSLGRQ SSIYSLTLDE    50
    FQHALCENGK NFGSMNMDEF LVSIWNAEEN NNNQQQAAAA AGSHSVPANH 100
    NGFNNNNNNG GEGGVGVFSG GSRGNEDANN KRGIANESSL PRQGSLTLPA 150
    PLCRKTVDEV WSEIHRGGGS GNGGDSNGRS SSSNGQNNAQ NGGETAARQP 200
    TFGEMTLEDF LVKAGVVREH PTNPKPNPNP NQNQNPSSVI PAAAQQQLYG 250
    VFQGTGDPSF PGQAMGVGDP SGYAKRTGGG GYQQAPPVQA GVCYGGGVGF 300
    GAGGQQMGMV GPLSPVSSDG LGHGQVDNIG GQYGVDMGGL RGRKRVVDGP 350
    VEKVVERRQR RMIKNRESAA RSRARKQAYT VELEAELNQL KEENAQLKHA 400
    LAELERKRKQ QYFESLKSRA QPKLPKSNGR LRTLMRNPSC PL 442
    Length:442
    Mass (Da):47,007
    Last modified:May 1, 2000 - v1
    Checksum:iF879F51B99A99140
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF334206 mRNA. Translation: AAK19599.1.
    AC006921 Genomic DNA. Translation: AAD21438.1.
    CP002685 Genomic DNA. Translation: AEC09226.1.
    BT026517 mRNA. Translation: ABH04624.1.
    PIRiG84778.
    RefSeqiNP_565840.1. NM_129185.3.
    UniGeneiAt.14114.

    Genome annotation databases

    EnsemblPlantsiAT2G36270.1; AT2G36270.1; AT2G36270.
    GeneIDi818199.
    KEGGiath:AT2G36270.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF334206 mRNA. Translation: AAK19599.1 .
    AC006921 Genomic DNA. Translation: AAD21438.1 .
    CP002685 Genomic DNA. Translation: AEC09226.1 .
    BT026517 mRNA. Translation: ABH04624.1 .
    PIRi G84778.
    RefSeqi NP_565840.1. NM_129185.3.
    UniGenei At.14114.

    3D structure databases

    ProteinModelPortali Q9SJN0.
    SMRi Q9SJN0. Positions 360-405.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 3543. 47 interactions.
    DIPi DIP-40551N.
    IntActi Q9SJN0. 7 interactions.
    STRINGi 3702.AT2G36270.1-P.

    Proteomic databases

    PRIDEi Q9SJN0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G36270.1 ; AT2G36270.1 ; AT2G36270 .
    GeneIDi 818199.
    KEGGi ath:AT2G36270.

    Organism-specific databases

    TAIRi AT2G36270.

    Phylogenomic databases

    eggNOGi NOG251869.
    HOGENOMi HOG000237278.
    InParanoidi Q9SJN0.
    KOi K14432.
    OMAi CENGKNF.
    PhylomeDBi Q9SJN0.

    Gene expression databases

    Genevestigatori Q9SJN0.

    Family and domain databases

    InterProi IPR004827. bZIP.
    [Graphical view ]
    Pfami PF00170. bZIP_1. 1 hit.
    [Graphical view ]
    SMARTi SM00338. BRLZ. 1 hit.
    [Graphical view ]
    PROSITEi PS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor."
      Finkelstein R.R., Lynch T.J.
      Plant Cell 12:599-609(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    2. "Arabidopsis ABI5 subfamily members have distinct DNA-binding and transcriptional activities."
      Kim S.Y., Ma J., Perret P., Li Z., Thomas T.L.
      Plant Physiol. 130:688-697(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY, DNA-BINDING, HETERODIMERIZATION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Arabidopsis ORF Clones."
      Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana."
      Lopez-Molina L., Chua N.H.
      Plant Cell Physiol. 41:541-547(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    7. "Physical interactions between ABA response loci of Arabidopsis."
      Nakamura S., Lynch T.J., Finkelstein R.R.
      Plant J. 26:627-635(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ABI3, HOMODIMERIZATION.
    8. "A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis."
      Lopez-Molina L., Mongrand S., Chua N.-H.
      Proc. Natl. Acad. Sci. U.S.A. 98:4782-4787(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, PHOSPHORYLATION.
    9. "The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis."
      Bensmihen S., Rippa S., Lambert G., Jublot D., Pautot V., Granier F., Giraudat J., Parcy F.
      Plant Cell 14:1391-1403(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.
    10. Cited for: FUNCTION, DNA-BINDING.
    11. "ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination."
      Lopez-Molina L., Mongrand S., McLachlin D.T., Chait B.T., Chua N.-H.
      Plant J. 32:317-328(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING, PHOSPHORYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
    12. "Regulation and role of the Arabidopsis abscisic acid-insensitive 5 gene in abscisic acid, sugar, and stress response."
      Brocard I.M., Lynch T.J., Finkelstein R.R.
      Plant Physiol. 129:1533-1543(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    13. "Mitogen-activated protein kinase signaling in postgermination arrest of development by abscisic acid."
      Lu C., Han M.-H., Guevara-Garcia A., Fedoroff N.V.
      Proc. Natl. Acad. Sci. U.S.A. 99:15812-15817(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION.
    14. Cited for: GENE FAMILY, NOMENCLATURE.
    15. "AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation."
      Lopez-Molina L., Mongrand S., Kinoshita N., Chua N.-H.
      Genes Dev. 17:410-418(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN DEGRADATION, INTERACTION WITH AFP1.
    16. "The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling."
      Smalle J., Kurepa J., Yang P., Emborg T.J., Babiychuk E., Kushnir S., Vierstra R.D.
      Plant Cell 15:965-980(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN DEGRADATION.
    17. "Three genes that affect sugar sensing (abscisic acid insensitive 4, abscisic acid insensitive 5, and constitutive triple response 1) are differentially regulated by glucose in Arabidopsis."
      Arroyo A., Bossi F., Finkelstein R.R., Leon P.
      Plant Physiol. 133:231-242(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    18. "Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence."
      Pourtau N., Mares M., Purdy S., Quentin N., Rueel A., Wingler A.
      Planta 219:765-772(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    19. "Redundant and distinct functions of the ABA response loci ABA-INSENSITIVE(ABI)5 and ABRE-BINDING FACTOR (ABF)3."
      Finkelstein R.R., Gampala S.S., Lynch T.J., Thomas T.L., Rock C.D.
      Plant Mol. Biol. 59:253-267(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ABI3.
    20. "KEEP ON GOING, a RING E3 ligase essential for Arabidopsis growth and development, is involved in abscisic acid signaling."
      Stone S.L., Williams L.A., Farmer L.M., Vierstra R.D., Callis J.
      Plant Cell 18:3415-3428(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN DEGRADATION, INTERACTION WITH KEG.
    21. "Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis."
      Nakashima K., Fujita Y., Katsura K., Maruyama K., Narusaka Y., Seki M., Shinozaki K., Yamaguchi-Shinozaki K.
      Plant Mol. Biol. 60:51-68(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    22. "Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis."
      Fujii H., Verslues P.E., Zhu J.-K.
      Plant Cell 19:485-494(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY SRK2D AND SRK2I.
    23. "A small plant-specific protein family of ABI five binding proteins (AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings."
      Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R.
      Plant Mol. Biol. 67:643-658(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AFP1; AFP2; AFP3 AND AFP4.
    24. "Integration of light and abscisic acid signaling during seed germination and early seedling development."
      Chen H., Zhang J., Neff M.M., Hong S.-W., Zhang H., Deng X.-W., Xiong L.
      Proc. Natl. Acad. Sci. U.S.A. 105:4495-4500(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    25. "Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling."
      Miura K., Lee J., Jin J.B., Yoo C.Y., Miura T., Hasegawa P.M.
      Proc. Natl. Acad. Sci. U.S.A. 106:5418-5423(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUMOYLATION AT LYS-391, MUTAGENESIS OF LYS-391.
    26. "The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors."
      Chen R., Jiang H., Li L., Zhai Q., Qi L., Zhou W., Liu X., Li H., Zheng W., Sun J., Li C.
      Plant Cell 24:2898-2916(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED25.

    Entry informationi

    Entry nameiABI5_ARATH
    AccessioniPrimary (citable) accession number: Q9SJN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3