Reviewed, UniProtKB/Swiss-Prot Q9SJM0 (CALSA_ARATH)

Names and origin

Protein names

Recommended name:
    Callose synthase 10
    EC=2.4.1.34
Alternative name(s):
    1,3-beta-glucan synthase
    Protein GLUCAN SYNTHASE-LIKE 8

Gene names

Name:	CALS10
Synonyms:	GSL8
Ordered Locus Names:	At2g36850
ORF Names:	T1J8.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1675 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in sporophytic and gametophytic development. Required for normal plant development. During pollen formation, required for the entry of microspores into mitosis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals. Ref.5
Catalytic activity	UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Developmental stage	Expressed throughout pollen development with a at mature pollen stage. Ref.5
Disruption phenotype	Plants develop collapsed and inviable pollen grains. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 48 family. Contains 3 HAT repeats. Contains 3 LRR (leucine-rich) repeats.
Sequence caution	The sequence AAD31571.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Leucine-rich repeat Repeat Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	1,3-beta-glucan biosynthetic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	1,3-beta-glucan synthase complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,3-beta-glucan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1675	1675	Callose synthase 10		PRO_0000334582
Regions
	Transmembrane	425 – 445	21	Potential
	Transmembrane	458 – 478	21	Potential
	Transmembrane	498 – 518	21	Potential
	Transmembrane	1265 – 1285	21	Potential
	Transmembrane	1320 – 1340	21	Potential
	Transmembrane	1345 – 1365	21	Potential
	Transmembrane	1412 – 1432	21	Potential
	Transmembrane	1435 – 1455	21	Potential
	Transmembrane	1509 – 1529	21	Potential
	Transmembrane	1549 – 1569	21	Potential
	Transmembrane	1582 – 1602	21	Potential
	Transmembrane	1624 – 1644	21	Potential
	Repeat	24 – 56	33	HAT 1
	Repeat	527 – 550	24	LRR 1
	Repeat	737 – 760	24	LRR 2
	Repeat	886 – 919	34	HAT 2
	Repeat	971 – 993	23	LRR 3
	Repeat	1450 – 1482	33	HAT 3
Experimental info
	Sequence conflict	1601 – 1602	2	TW → YG in BAF01442. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9SJM0-1 [UniParc]. Last modified July 22, 2008. Version 3. Checksum: AEFFC61260678902	FASTA	1,675	191,238
	10 20 30 40 50 60 MAQNLDPNSD GRGVLQFKTG LMSVIKQKLA KRDGASIDRD RDIERLWEFY KLYKRRHRVD 70 80 90 100 110 120 DIQKEEQKWR ESGTTFSSNV GEILKMRKVF ATLRALIEVL EVLSRDADPN GVGRSIRDEL 130 140 150 160 170 180 GRIKKADATL SAELTPYNIV PLEAQSMTNA IGVFPEVRGA VQAIRYTEHF PRLPVDFEIS 190 200 210 220 230 240 GQRDADMFDL LEYIFGFQRD NVRNQREHLV LTLSNAQSQL SIPGQNDPKI DENAVNEVFL 250 260 270 280 290 300 KVLDNYIKWC KYLRIRVVYN KLEAIDRDRK LFLVSLYFLI WGEAANVRFL PECICYIFHN 310 320 330 340 350 360 MAKELDAKLD HGEAVRADSC LTGTDTGSVS FLERIICPIY ETISAETVRN NGGKAAHSEW 370 380 390 400 410 420 RNYDDFNEYF WTPACFELSW PMKTESRFLS KPKGRKRSLT IIAFRNEHLN IETFKILLSA 430 440 450 460 470 480 GPTYAIMNFI ECLLDVVLMY GAYSMARGMA ISRLVIRYVA FWLVVLASKF TFAYFLQARS 490 500 510 520 530 540 FYSSNDHALT IVSLWAPVLA IYLMDIHIWY TLLSAIIGGV MGAKARLGEI RTIEMVHKRF 550 560 570 580 590 600 ESFPEAFAQN LVSPVVKRVP LGQHASQVSD KFKLSVLSLD VLIDILALLP YIDGQDMNKA 610 620 630 640 650 660 YAAMFSPFWN EIIKSLREED YLSNREMDLL SIPSNTGSLR LVQWPLFLLC SKILVAIDLA 670 680 690 700 710 720 MECKETQEVL WRQICDDEYM AYAVQECYYS VEKILNSMVN DEGRRWYSIS VCLNLNVCLI 730 740 750 760 770 780 CEDHISNSIE QGSLAITLNL KKLQLVVSRF TALTGLLIRN ETPDLAKGAA KAMFDFYEVV 790 800 810 820 830 840 THDLLSHDLR EQLDTWNILA RARNEGRLFS RIAWPRDPEI IEQVKRLHLL LTVKDAAANV 850 860 870 880 890 900 PKNLEARRRL EFFTNSLFMD MPQARPVAEM VPFSVFTPYY SETVLYSSSE LRSENEDGIS 910 920 930 940 950 960 ILFYLQKIFP DEWENFLERI GRSESTGDAD LQASSTDALE LRFWVSYRGQ TLARTVRGMM 970 980 990 1000 1010 1020 YYRRALMLQS FLERRGLGVD DASLTNMPRG FESSIEARAQ ADLKFTYVVS CQIYGQQKQQ 1030 1040 1050 1060 1070 1080 KKPEATDIGL LLQRYEALRV AFIHSEDVGN GDGGSGGKKE FYSKLVKADI HGKDEEIYSI 1090 1100 1110 1120 1130 1140 KLPGDPKLGE GKPENQNHAI VFTRGEAIQT IDMNQDNYLE EAIKMRNLLE EFHGKHGIRR 1150 1160 1170 1180 1190 1200 PTILGVREHV FTGSVSSLAW FMSNQETSFV TLGQRVLAYP LKVRMHYGHP DVFDRIFHIT 1210 1220 1230 1240 1250 1260 RGGISKASRV INISEDIYAG RDVGLNQIAL FEGKVAGGNG EQVLSRDVYR IGQLFDFFRM 1270 1280 1290 1300 1310 1320 MSFYFTTVGF YVCTMMTVLT VYVFLYGRVY LAFSGADRAI SRVAKLSGNT ALDAALNAQF 1330 1340 1350 1360 1370 1380 LVQIGIFTAV PMVMGFILEL GLLKAIFSFI TMQFQLCSVF FTFSLGTRTH YFGRTILHGG 1390 1400 1410 1420 1430 1440 AKYRATGRGF VVQHIKFADN YRLYSRSHFV KAFEVALLLI IYIAYGYTDG GASSFVLLTI 1450 1460 1470 1480 1490 1500 SSWFLVISWL FAPYIFNPSG FEWQKTVEDF EDWVSWLMYK GGVGVKGELS WESWWEEEQA 1510 1520 1530 1540 1550 1560 HIQTLRGRIL ETILSLRFFM FQYGIVYKLD LTRKNTSLAL YGYSWVVLVV IVFLFKGVAS 1570 1580 1590 1600 1610 1620 ITFIALIVVA IAMTDLSIPD MFACVLGFIP TGWALLSLAI TWKQVLRVLG LWETVREFGR 1630 1640 1650 1660 1670 IYDAAMGMLI FSPIALLSWF PFISTFQSRL LFNQAFSRGL EISIILAGNR ANVET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1601-1675. Strain: cv. Columbia.
[3]	"A cell plate-specific callose synthase and its interaction with phragmoplastin." Hong Z., Delauney A.J., Verma D.P.S. Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract] Cited for: GENE FAMILY AND NOMENCLATURE.
[4]	"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility." Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E. Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract] Cited for: NOMENCLATURE.
[5]	"Dual function of Arabidopsis glucan synthase-like genes GSL8 and GSL10 in male gametophyte development and plant growth." Toeller A., Brownfield L., Neu C., Twell D., Schulze-Lefert P. Plant J. 54:911-923(2008) [PubMed: 18315544] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC006922 Genomic DNA. Translation: AAD31571.1. Sequence problems. AK229594 mRNA. Translation: BAF01442.1.
IPI	IPI00532489.
PIR	E84785.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT48. Glycosyltransferase Family 48.
Proteomic databases
PRIDE	Q9SJM0.
Genome annotation databases
GenomeReviews	Gene locus AT2G36850 in contig CT485783_GR.
Organism-specific databases
TAIR	At2g36850.
Gene expression databases
ArrayExpress	Q9SJM0.
Family and domain databases
InterPro	IPR001810. F-box. IPR003440. Glyco_trans_48. [Graphical view]
Pfam	PF00646. F-box. 1 hit. PF02364. Glucan_synthase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALSA_ARATH

Accession

Primary (citable) accession number: Q9SJM0
Secondary accession number(s): Q0WN58

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 22, 2008
Last modified:	June 16, 2009
This is version 41 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)