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Q9SJM0 (CALSA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Callose synthase 10
EC=2.4.1.34
Alternative name(s):
1,3-beta-glucan synthase
Protein CHORUS
Protein GLUCAN SYNTHASE-LIKE 8
Gene names
Name:CALS10
Synonyms:GSL8
Ordered Locus Names:At2g36850
ORF Names:T1J8.3
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length1904 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in sporophytic and gametophytic development. Required for normal plant development and for the proper accumulation of callose at cell plates, cll walls and plasmodesmata. During pollen formation, required for the entry of microspores into mitosis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals. Required for proper cell division and tissue patterning throughout plant organs, including stomatal patterning. Ref.1 Ref.7

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Developmental stage

Expressed throughout pollen development with a at mature pollen stage. Ref.7

Disruption phenotype

Seedling lethality with severe dwarfism. Plants develop collapsed and inviable pollen grains. Ref.1 Ref.7

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Contains 3 HAT repeats.

Contains 4 LRR (leucine-rich) repeats.

Sequence caution

The sequence AAD31571.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19041904Callose synthase 10
PRO_0000334582

Regions

Transmembrane491 – 51121Helical; Potential
Transmembrane532 – 55221Helical; Potential
Transmembrane562 – 58221Helical; Potential
Transmembrane594 – 61421Helical; Potential
Transmembrane661 – 68121Helical; Potential
Transmembrane722 – 74221Helical; Potential
Transmembrane1474 – 149421Helical; Potential
Transmembrane1529 – 154921Helical; Potential
Transmembrane1554 – 157421Helical; Potential
Transmembrane1621 – 164121Helical; Potential
Transmembrane1644 – 166421Helical; Potential
Transmembrane1747 – 176721Helical; Potential
Transmembrane1783 – 180321Helical; Potential
Transmembrane1811 – 183121Helical; Potential
Transmembrane1853 – 187321Helical; Potential
Repeat100 – 13233HAT 1
Repeat678 – 70124LRR 1
Repeat751 – 77424LRR 2
Repeat925 – 94824LRR 3
Repeat1074 – 110734HAT 2
Repeat1159 – 118123LRR 4
Repeat1659 – 169133HAT 3

Experimental info

Sequence conflict1830 – 18312TW → YG in BAF01442. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9SJM0 [UniParc].

Last modified January 25, 2012. Version 5.
Checksum: 7FC337410084EFCF

FASTA1,904218,378
        10         20         30         40         50         60 
MARVYSNWDR LVRATLRREQ LRNTGQGHER VSSGLAGAVP PSLGRATNID AILQAADEIQ 

        70         80         90        100        110        120 
SEDPSVARIL CEQAYSMAQN LDPNSDGRGV LQFKTGLMSV IKQKLAKRDG ASIDRDRDIE 

       130        140        150        160        170        180 
RLWEFYKLYK RRHRVDDIQK EEQKWRESGT TFSSNVGEIL KMRKVFATLR ALIEVLEVLS 

       190        200        210        220        230        240 
RDADPNGVGR SIRDELGRIK KADATLSAEL TPYNIVPLEA QSMTNAIGVF PEVRGAVQAI 

       250        260        270        280        290        300 
RYTEHFPRLP VDFEISGQRD ADMFDLLEYI FGFQRDNVRN QREHLVLTLS NAQSQLSIPG 

       310        320        330        340        350        360 
QNDPKIDENA VNEVFLKVLD NYIKWCKYLR IRVVYNKLEA IDRDRKLFLV SLYFLIWGEA 

       370        380        390        400        410        420 
ANVRFLPECI CYIFHNMAKE LDAKLDHGEA VRADSCLTGT DTGSVSFLER IICPIYETIS 

       430        440        450        460        470        480 
AETVRNNGGK AAHSEWRNYD DFNEYFWTPA CFELSWPMKT ESRFLSKPKG RKRTAKSSFV 

       490        500        510        520        530        540 
EHRTYLHLFR SFIRLWIFMF IMFQSLTIIA FRNEHLNIET FKILLSAGPT YAIMNFIECL 

       550        560        570        580        590        600 
LDVVLMYGAY SMARGMAISR LVIRFLWWGL GSAFVVYYYV KVLDERNKPN QNEFFFHLYI 

       610        620        630        640        650        660 
LVLGCYAAVR LIFGLLVKLP ACHALSEMSD QSFFQFFKWI YQERYFVGRG LFENLSDYCR 

       670        680        690        700        710        720 
YVAFWLVVLA SKFTFAYFLQ IKPLVKPTNT IIHLPPFQYS WHDIVSKSND HALTIVSLWA 

       730        740        750        760        770        780 
PVLAIYLMDI HIWYTLLSAI IGGVMGAKAR LGEIRTIEMV HKRFESFPEA FAQNLVSPVV 

       790        800        810        820        830        840 
KRVPLGQHAS QDGQDMNKAY AAMFSPFWNE IIKSLREEDY LSNREMDLLS IPSNTGSLRL 

       850        860        870        880        890        900 
VQWPLFLLCS KILVAIDLAM ECKETQEVLW RQICDDEYMA YAVQECYYSV EKILNSMVND 

       910        920        930        940        950        960 
EGRRWVERIF LEISNSIEQG SLAITLNLKK LQLVVSRFTA LTGLLIRNET PDLAKGAAKA 

       970        980        990       1000       1010       1020 
MFDFYEVVTH DLLSHDLREQ LDTWNILARA RNEGRLFSRI AWPRDPEIIE QVKRLHLLLT 

      1030       1040       1050       1060       1070       1080 
VKDAAANVPK NLEARRRLEF FTNSLFMDMP QARPVAEMVP FSVFTPYYSE TVLYSSSELR 

      1090       1100       1110       1120       1130       1140 
SENEDGISIL FYLQKIFPDE WENFLERIGR SESTGDADLQ ASSTDALELR FWVSYRGQTL 

      1150       1160       1170       1180       1190       1200 
ARTVRGMMYY RRALMLQSFL ERRGLGVDDA SLTNMPRGFE SSIEARAQAD LKFTYVVSCQ 

      1210       1220       1230       1240       1250       1260 
IYGQQKQQKK PEATDIGLLL QRYEALRVAF IHSEDVGNGD GGSGGKKEFY SKLVKADIHG 

      1270       1280       1290       1300       1310       1320 
KDEEIYSIKL PGDPKLGEGK PENQNHAIVF TRGEAIQTID MNQDNYLEEA IKMRNLLEEF 

      1330       1340       1350       1360       1370       1380 
HGKHGIRRPT ILGVREHVFT GSVSSLAWFM SNQETSFVTL GQRVLAYPLK VRMHYGHPDV 

      1390       1400       1410       1420       1430       1440 
FDRIFHITRG GISKASRVIN ISEDIYAGFN STLRQGNITH HEYIQVGKGR DVGLNQIALF 

      1450       1460       1470       1480       1490       1500 
EGKVAGGNGE QVLSRDVYRI GQLFDFFRMM SFYFTTVGFY VCTMMTVLTV YVFLYGRVYL 

      1510       1520       1530       1540       1550       1560 
AFSGADRAIS RVAKLSGNTA LDAALNAQFL VQIGIFTAVP MVMGFILELG LLKAIFSFIT 

      1570       1580       1590       1600       1610       1620 
MQFQLCSVFF TFSLGTRTHY FGRTILHGGA KYRATGRGFV VQHIKFADNY RLYSRSHFVK 

      1630       1640       1650       1660       1670       1680 
AFEVALLLII YIAYGYTDGG ASSFVLLTIS SWFLVISWLF APYIFNPSGF EWQKTVEDFE 

      1690       1700       1710       1720       1730       1740 
DWVSWLMYKG GVGVKGELSW ESWWEEEQAH IQTLRGRILE TILSLRFFMF QYGIVYKLDL 

      1750       1760       1770       1780       1790       1800 
TRKNTSLALY GYSWVVLVVI VFLFKLFWYS PRKSSNILLA LRFLQGVASI TFIALIVVAI 

      1810       1820       1830       1840       1850       1860 
AMTDLSIPDM FACVLGFIPT GWALLSLAIT WKQVLRVLGL WETVREFGRI YDAAMGMLIF 

      1870       1880       1890       1900 
SPIALLSWFP FISTFQSRLL FNQAFSRGLE ISIILAGNRA NVET

« Hide

References

« Hide 'large scale' references
[1]"Dysregulation of cell-to-cell connectivity and stomatal patterning by loss-of-function mutation in Arabidopsis CHORUS (GLUCAN SYNTHASE-LIKE 8)."
Guseman J.M., Lee J.S., Bogenschutz N.L., Peterson K.M., Virata R.E., Xie B., Kanaoka M.M., Hong Z., Torii K.U.
Development 137:1731-1741(2010) [PubMed: 20430748] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1830-1904.
Strain: cv. Columbia.
[5]"A cell plate-specific callose synthase and its interaction with phragmoplastin."
Hong Z., Delauney A.J., Verma D.P.S.
Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract]
Cited for: GENE FAMILY AND NOMENCLATURE.
[6]"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract]
Cited for: NOMENCLATURE.
[7]"Dual function of Arabidopsis glucan synthase-like genes GSL8 and GSL10 in male gametophyte development and plant growth."
Toeller A., Brownfield L., Neu C., Twell D., Schulze-Lefert P.
Plant J. 54:911-923(2008) [PubMed: 18315544] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		GQ373182 mRNA. Translation: ACV04899.1.
AC006922 Genomic DNA. Translation: AAD31571.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09306.1.
AK229594 mRNA. Translation: BAF01442.1.
IPIIPI00532489.
IPI01020464.
PIRE84785.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ9SJM0.

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Proteomic databases

PRIDEQ9SJM0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G36850.1; AT2G36850.1; AT2G36850.
GenomeReviewsGene locus AT2G36850 in contig CT485783_GR.

Organism-specific databases

TAIRAt2g36850.

Phylogenomic databases

eggNOGKOG0916.
GeneTreeEPGT00070000028111.
HOGENOMHBG316390.
PhylomeDBQ9SJM0.
ProtClustDBCLSN2704898.

Gene expression databases

ArrayExpressQ9SJM0.
GenevestigatorQ9SJM0.

Family and domain databases

InterProIPR003440. Glyco_trans_48.
[Graphical view]
PfamPF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCALSA_ARATH
AccessionPrimary (citable) accession number: Q9SJM0
Secondary accession number(s): C8C9X2, Q0WN58
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 25, 2012
Last modified: January 25, 2012
This is version 59 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents